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Experimental validation of a new morphing trailing edge system using Price-Pa?doussis wind tunnel tests 被引量:8
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作者 D.COMMUNIER R.M.BOTEZ T.WONG 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2019年第6期1353-1366,共14页
This paper presents the design and manufacturing of a new morphing wing system carried out at the Laboratory of Applied Research in Active Controls, Avionics and AeroServoElasticity(LARCASE) at the ETS in Montréa... This paper presents the design and manufacturing of a new morphing wing system carried out at the Laboratory of Applied Research in Active Controls, Avionics and AeroServoElasticity(LARCASE) at the ETS in Montréal. This first version of a morphing wing allows the deformation of its trailing edge, denote by Morphing Trailing Edge(MTE). In order to characterize the technical impact of this deformation, we compare its performance with that of a rigid aileron by testing in the LARCASE’s price-Pa?doussis subsonic wind tunnel. The first set of results shows that it is possible to replace an aileron by a MTE on a wing, as an improvement was observed for the MTE aerodynamic performances with respect to the aileron aerodynamic performances.The improvement consisted in the fact that the drag coefficient was smaller, and the lift-to-drag ratio was higher for the same lift coefficient. 展开更多
关键词 Aerodynamic PERFORMANCES AILERON MORPHING WING trailing edge WING TUNNEL tests
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Modeling and Experiment of a Morphing Wing Integrated with a Trailing Edge Control Actuation System 被引量:1
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作者 HE Yuanyuan GUO Shijun 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2012年第2期248-254,共7页
Morphing wing has attracted many research attention and effort in aircraft technology development because of its advantage in lift to draft ratio and flight performance.Morphing wing technology combines the lift and c... Morphing wing has attracted many research attention and effort in aircraft technology development because of its advantage in lift to draft ratio and flight performance.Morphing wing technology combines the lift and control surfaces into a seamless wing and integrates the primary structure together with the internal control system.It makes use of the wing aeroelastic deformation induced by the control surface to gain direct force control through desirable redistribution of aerodynamic forces.However some unknown mechanical parameters of the control system and complexity of the integrated structure become a main challenge for dynamic modeling of morphing wing.To solve the problem,a method of test data based modal sensitivity analysis is presented to improve the morphing wing FE model by evaluating the unknown parameters and identifying the modeling boundary conditions.An innovative seamless morphing wing with the structure integrated with a flexible trailing edge control system is presented for the investigation.An experimental model of actuation system driven by a servo motor for the morphing wing is designed and established.By performing a vibration test and the proposed modal sensitivity analysis,the unknown torsional stiffness of the servo motor and the boundary condition of the actuation mechanism model is identified and evaluated.Comparing with the test data,the average error of the first four modal frequency of the improved FE model is reduced significantly to less than 4%.To further investigate the morphing wing modeling,a wing box and then a whole morphing wing model including the skin and integrated with the trailing edge actuation system are established and tested.By using the proposed method,the FE model is improved by relaxing the constraint between the skin and actuation mechanism.The results show that the average error of the first three modal frequency of the improved FE model is reduced to less than 6%.The research results demonstrate that the presented seamless morphing wing integrated with a flexible trailing edge control surface can improve aerodynamic characteristics.By using the test data based modal sensitivity analysis method,the unknown parameter and boundary condition of the actuation model can be determined to improve the FE model.The problem in dynamic modeling of high accuracy for a morphing wing can be solved in an effective manner. 展开更多
关键词 morphing wing seamless trailing edge control surface actuation system modeling and vibration test
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Flagstaff Urban Trails System and Its Inspiration for the Construction of China National Trails System 被引量:1
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作者 HU Haisheng ZHENG Yanping 《Journal of Landscape Research》 2014年第2期1-3,10,共4页
Flagstaff Urban Trails System(FUTS) is a city-wide network of non-motorized, shared-use pathways that are used by bicyclists, walkers, hikers, runners, and other users for both recreation and transportation. FUTS tota... Flagstaff Urban Trails System(FUTS) is a city-wide network of non-motorized, shared-use pathways that are used by bicyclists, walkers, hikers, runners, and other users for both recreation and transportation. FUTS totals 79.2 km in Flagstaff, the Flagstaff government approved the fi rst plan in 1988, and FUTS Trail Priority Evaluation 2011 outlined the evaluation criteria, trail rankings and ranking results The new FUTS Master Plan shows about 130-km planned trails, and creates an ultimate trail system about 210 km. FUTS management organization and Transportation Tax Program is the key to keep the successful works of FUTS. Status quo of China National Trails System(CNTS) is reviewed as: scenic areas have relatively sound trail systems, but there is no any standard system; green corridors within the urban and rural system become popular, but there is no connection system. The development of China National Trails System can be promoted in 3 aspects: establishing national trails system, planning and design trails comprehensively, stressing daily operation and management. 展开更多
关键词 trail Flagstaff Urban trails system(FUTS) China National trails system(CNTS)
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Exercise-based rehabilitation programmes for pulmonary hypertension:a systematic review and meta-analysis of randomized controlled trails
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作者 Lu Yan Qingyun Yan Zhihong Liu 《中国循环杂志》 CSCD 北大核心 2018年第S01期167-168,共2页
Objective Individuals with pulmonary hypertension(PH)have reduced exercise capacity and quality of life.Exercise-based rehabilitation may have beneficial effects to improve the exercise capacity and quality of life in... Objective Individuals with pulmonary hypertension(PH)have reduced exercise capacity and quality of life.Exercise-based rehabilitation may have beneficial effects to improve the exercise capacity and quality of life in patients with PH.We aim to assess the efficacy and safety of exercised-based rehabilitation for people eith PH by performing a randomized controlled trails(RCTs)meta-analysis of exercised-based rehabilitation for people with PH by performing a randomized controlled trails meta-analysis. 展开更多
关键词 PULMONARY hypertension RANDOMIZED CONTROLLED trailS trailS META-ANALYSIS
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Construction of Trail Tourist Attractions Assessment Index System
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作者 LI Chaojun 《Journal of Landscape Research》 2017年第1期61-64,70,共5页
Based on the further analysis of trail's concept,trail tourist attractions was proposed as a new concept,and the 5-dimension(trail resource,trail operation,trail technology,trail management and trail economic inde... Based on the further analysis of trail's concept,trail tourist attractions was proposed as a new concept,and the 5-dimension(trail resource,trail operation,trail technology,trail management and trail economic index) theoretical assessment framework for trail tourist attractions was established. Trail route selection,trail pavement and trail management grade were taken for example to explain the specifications for these secondary indexes. The future research should expand the index system to cover all secondary indexes of the 5 dimensions,put the concept of trail tourist attractions into tourism planning practices,and provide examples for the construction of trail tourist attractions across the country. Proposal of the concept of trail tourist attractions and its assessment standards is an attempt and innovation in the development of scenic areas,and will possibly result in the new development rules of scenic areas. 展开更多
关键词 trail trail tourist attractions Conceptual model Assessment index
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携带TRAIL基因的P28启动子溶瘤腺病毒的抗肝癌研究
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作者 金晓婷 刘新垣 +2 位作者 陈若愚 李宁 章康健 《中国细胞生物学学报》 2025年第4期767-776,共10页
P28(也被称为PSMD10、P28^(GANK)或gankyrin)在肝癌中高表达(96.9%)而在正常细胞中不表达,因此利用P28启动子设计了具有肝癌靶向性的新型溶瘤病毒载体并在此基础上设计了携带肿瘤坏死因子(tumor necrosis factor,TNF)相关凋亡诱导配体(T... P28(也被称为PSMD10、P28^(GANK)或gankyrin)在肝癌中高表达(96.9%)而在正常细胞中不表达,因此利用P28启动子设计了具有肝癌靶向性的新型溶瘤病毒载体并在此基础上设计了携带肿瘤坏死因子(tumor necrosis factor,TNF)相关凋亡诱导配体(TNF related apoptosis inducing ligand,TRAIL)基因的溶瘤腺病毒(Onco^(Ad)-P28-E1A-?E1B-TRAIL)。通过Onco^(Ad)-P28-TRAIL感染肝癌细胞显示TRAIL蛋白可以在肝癌细胞中高效表达。结晶紫染色显示该病毒可以选择性靶向杀伤肝癌细胞,且10 MOI对人正常肝细胞没有明显毒性。体外细胞水平杀伤及凋亡研究显示Onco~(Ad)-P28-TRAIL对肝癌细胞Huh-7和Hep-3B均有抑制作用,且呈时间和剂量依赖性,可以显著促进肝癌细胞凋亡。皮下移植瘤CDX药效显示该溶瘤腺病毒可以促进肿瘤细胞的凋亡及免疫细胞的浸润从而显著抑制肝癌细胞皮下瘤的生长。这些结果提示溶瘤腺病毒Onco^(Ad)-P28-TRAIL能有效抑制肝癌的发展,是一种具有临床应用潜力的肝癌治疗候选药物。 展开更多
关键词 P28启动子 trail 溶瘤腺病毒 肝癌
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Interference mechanism of trailing edge flap shedding vortices with rotor wake and aerodynamic characteristics
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作者 Yang LIU Yongjie SHI +2 位作者 Aqib AZIZ Guohua XU Haifeng GAO 《Chinese Journal of Aeronautics》 2025年第1期325-339,共15页
A robust Reynolds-Averaged Navier-Stokes(RANS)based solver is established to predict the complex unsteady aerodynamic characteristics of the Active Flap Control(AFC)rotor.The complex motion with multiple degrees of fr... A robust Reynolds-Averaged Navier-Stokes(RANS)based solver is established to predict the complex unsteady aerodynamic characteristics of the Active Flap Control(AFC)rotor.The complex motion with multiple degrees of freedom of the Trailing Edge Flap(TEF)is analyzed by employing an inverse nested overset grid method.Simulation of non-rotational and rotational modes of blade motion are carried out to investigate the formation and development of TEF shedding vortex with high-frequency deflection of TEF.Moreover,the mechanism of TEF deflection interference with blade tip vortex and overall rotor aerodynamics is also explored.In nonrotational mode,two bundles of vortices form at the gap ends of TEF and the main blade and merge into a single TEF vortex.Dynamic deflection of the TEF significantly interferes with the blade tip vortex.The position of the blade tip vortex consistently changes,and its frequency is directly related to the frequency of TEF deflection.In rotational mode,the tip vortex forms a helical structure.The end vortices at the gap sides co-swirl and subsequently merge into the concentrated beam of tip vortices,causing fluctuations in the vorticity and axial position of the tip vortex under the rotor.This research concludes with the investigation on suppression of Blade Vortex Interaction(BVI),showing an increase in miss distance and reduction in the vorticity of tip vortex through TEF phase control at a particular control frequency.Through this mechanism,a designed TEF deflection law increases the miss distance by 34.7%and reduces vorticity by 11.9%at the target position,demonstrating the effectiveness of AFC in mitigating BVI. 展开更多
关键词 CFD ROTOR Aerodynamic trailing edge flap Vortex shedding BVI
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Vortex-Induced Vibration and Frequency Lock-In of an Elastically Suspended Hydrofoil with Blunt Trailing Edge
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作者 QIN Guangfei ZHANG Huaixin LI Date 《Journal of Shanghai Jiaotong university(Science)》 2025年第6期1289-1298,共10页
Vortex-induced vibration of hydrofoils is concerned with structural safety and noise level in hydraulic machinery and marine engineering.The research on vibration characteristics under different operating conditions i... Vortex-induced vibration of hydrofoils is concerned with structural safety and noise level in hydraulic machinery and marine engineering.The research on vibration characteristics under different operating conditions is significant.In this study,numerical simulations are conducted to investigate the vortex-induced vibration responses of an elastically suspended hydrofoil with blunt trailing edge in pitch direction.The work studies the effects of four parameters,namely the structural natural frequency,mass ratio,initial attack angle,and Reynolds number on vibration characteristics,with special emphasis on frequency lock-in.Results indicate that as the structural natural frequency changes,the vibration amplitude may increase substantially within a certain frequency range,in which the vortex shedding frequency locks into the structural natural frequency,and frequency lock-in occurs.In addition,with increasing the mass ratio,the frequency range of lock-in becomes narrower,and both the upper and lower thresholds decrease.As the initial attack angle increases from 0◦to 6◦,the lock-in range gets reduced.Over the three Reynolds numbers(6×10^(5),9×10^(5),and 12×10^(5)),the lock-in range remains virtually unchanged.Moreover,for a certain structural natural frequency,modifying the mass ratio,initial attack angle,and Reynolds number could effectively suppress the vibration amplitude. 展开更多
关键词 vortex-induced vibration HYDROFOIL blunt trailing edge frequency lock-in structural natural frequency mass ratio initial attack angle Reynolds number
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Bioinspired Trailing Edge Serrations for Vertical Axis Wind Turbine Blades in Urban Environments: Performance Effects
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作者 Luis Santamaria Laura Suarez Fernandez +2 位作者 Manuel Garcia-Diaz Jose Gonzalez Perez Monica Galdo 《Journal of Bionic Engineering》 2025年第2期822-837,共16页
Biomimetics has recently emerged as an interesting approach to enhance renewable energy technologies.In this work,bioinspired Trailing Edge Serrations(TES)were evaluated on a typical Vertical Axis Wind Turbine(VAWT)ai... Biomimetics has recently emerged as an interesting approach to enhance renewable energy technologies.In this work,bioinspired Trailing Edge Serrations(TES)were evaluated on a typical Vertical Axis Wind Turbine(VAWT)airfoil,the DU06-W200.As noise reduction benefits of these mechanisms are already well-established,this study focuses on their impact on airfoil and VAWT performance.A saw-tooth geometry was chosen based on VAWT specifications and existing research,followed by a detailed assessment through wind tunnel tests using a newly developed aerodynamic balance.For a broad spectrum of attack angles and Reynolds numbers,lift,drag,and pitching moments were carefully measured.The results show that TES enhance the lift-to-drag ratio,especially in stalled conditions,and postpone stall at negative angles,expanding the effective performance range.A notable increase in pitching moment also is also observed,relevant for blade-strut joint design.Additionally,the impact on turbine performance was estimated using an analytical model,demonstrating excellent accuracy when compared against previous experimental results.TES offer a modest 2%improve-ment in peak performance,though they slightly narrow the optimal tip-speed ratio zone.Despite this,the potential noise reduction and performance gains make TES a valuable addition to VAWT designs,especially in urban settings. 展开更多
关键词 Biomimetics trailing edge serrations Passive flow control device Vertical axis wind turbine VAWT Wind tunnel DU06-W200
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“两高”刑事司法解释冲突的现象、成因及其消解
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作者 洪浩 郑旺佳 《武汉大学学报(哲学社会科学版)》 北大核心 2026年第1期150-160,共11页
“两高”刑事司法解释冲突的现象时有发生,不仅因解释形式的不同可能导致的效力冲突,也存在解释对象、内容、方法等层面的冲突。表明冲突现象已从解释规范及其结论的冲突逐渐演变为解释起草者等认知视角的冲突,其本质源于司法解释主体... “两高”刑事司法解释冲突的现象时有发生,不仅因解释形式的不同可能导致的效力冲突,也存在解释对象、内容、方法等层面的冲突。表明冲突现象已从解释规范及其结论的冲突逐渐演变为解释起草者等认知视角的冲突,其本质源于司法解释主体之间的职能差异。究其原因,现行“二元一级”解释体制的局限性,决定了解释主体之间存在较大观点分歧;刑事诉讼中公诉权与审判权的职能互动关系,使得“两高”基于诉讼目的和治理责任的不同以发布司法解释;另有司法解释冲突解决的原则性条款虚置、司法解释冲突协调机制长期阙如等。故而,需构建以联合解释为主、遵循审判解释效力优先的解释体制,统一司法解释的普遍适用性,明确“两高”司法解释的内部效力位阶;建立司法解释冲突的内外协调机制,“两高”共同制定司法解释制发的工作规范,并确立“两高”司法解释冲突的备案审查机制,以激活立法解释对司法解释冲突的纠偏功能,进而达到消解“两高”刑事司法解释冲突之目的。 展开更多
关键词 司法解释冲突 二元一级制 审判解释 检察解释 联合解释 监察体制改革
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Fas FasL TRAIL分子在尖锐湿疣皮损中的分布 被引量:19
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作者 谢红付 冯浩 +3 位作者 范学工 肖满兰 陈翔 施为 《中国皮肤性病学杂志》 CAS 北大核心 2003年第3期151-153,共3页
目的 探讨Fas、FasL和TRAIL介导的细胞凋亡在尖锐湿疣发病中的可能作用及意义。方法 用免疫组化方法检测了 30例尖锐湿疣患者皮损中Fas、FasL和TRAIL的表达。结果 所有尖锐湿疣标本 ( 10 0 % )Fas均阳性 ,2 3例 ( 76 .7% )FasL阳性 ,... 目的 探讨Fas、FasL和TRAIL介导的细胞凋亡在尖锐湿疣发病中的可能作用及意义。方法 用免疫组化方法检测了 30例尖锐湿疣患者皮损中Fas、FasL和TRAIL的表达。结果 所有尖锐湿疣标本 ( 10 0 % )Fas均阳性 ,2 3例 ( 76 .7% )FasL阳性 ,2 1例 ( 70 .0 % )TRAIL阳性 ,三者分布基本一致 ,正常表皮基本不表达。且FasL和TRAIL分子与真皮单一核细胞浸润呈显著负相关 (γ =-0 .5 0 6和γ =-0 .45 0 ,P均 <0 .0 5 )。结论 尖锐湿疣组织中细胞凋亡相关分子Fas、FasL和TRAIL表达异常 ,且Fas、TRAIL分子表达影响局部免疫反应 ,考虑凋亡失控可能参与尖锐湿疣发病 ,引起复发。 展开更多
关键词 FAS FASL trail 尖锐湿疣 皮损 细胞凋亡 人乳头瘤病毒
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TRAIL受体在肿瘤细胞系上的表达及意义 被引量:8
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作者 韩丽辉 孙汶生 +3 位作者 马春红 张利宁 曹英林 宋静 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2002年第2期128-130,共3页
目的  检测TNF相关凋亡诱导配体(TRAIL)的受体,在来源于血液系统、肝脏、肺脏和大肠的8个肿瘤细胞系中的表达,并探讨其意义。方法采用半定量RT-PCR,对TRAIL受体的表达进行半定量检测。结果 TRAIL凋亡... 目的  检测TNF相关凋亡诱导配体(TRAIL)的受体,在来源于血液系统、肝脏、肺脏和大肠的8个肿瘤细胞系中的表达,并探讨其意义。方法采用半定量RT-PCR,对TRAIL受体的表达进行半定量检测。结果 TRAIL凋亡通路中,能够诱导凋亡反应的死亡受体DR4和DR5,在所检测的肿瘤细胞系中都有表达,其中DR5在所有肿瘤细胞系中的表达水平均显著高于DR4(P<0.05)。而能够竞争性阻断TRAIL诱导的凋亡反应的诱骗受体DcR1和DcR2,在所有的肿瘤细胞中都呈低水平表达或不表达。结论 DR5可能在TRAIL诱导凋亡的通路中发挥最重要的作用。TRAIL死亡受体和诱骗受体在肿瘤细胞系中的表达具有差异性,这种差异性可在一定程度上解释不同细胞对TRAIL诱导凋亡的敏感度。 展开更多
关键词 肿瘤 RT-PCR trail trail受体
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重组可溶性TRAIL的表达与生物学活性 被引量:16
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作者 史娟 张锦春 +3 位作者 畅晓燕 刘士廉 刘彦信 郑德先 《中国生物工程杂志》 CAS CSCD 2003年第6期46-49,共4页
研究重组可溶性TRAIL的抗肿瘤生物学活性。采用基因分子生物学方法构建重组可溶性TRAIL的表达载体 ,建立大肠杆菌表达菌株 ,采用柱层析等方法获得纯化的重组可溶性TRAIL ;采用流式细胞术、细胞活性测定法和体内药效学实验分析重组可溶性... 研究重组可溶性TRAIL的抗肿瘤生物学活性。采用基因分子生物学方法构建重组可溶性TRAIL的表达载体 ,建立大肠杆菌表达菌株 ,采用柱层析等方法获得纯化的重组可溶性TRAIL ;采用流式细胞术、细胞活性测定法和体内药效学实验分析重组可溶性TRAIL杀伤肿瘤细胞的生物学活性。结果表明重组可溶性TRAIL在体外可诱导人白血病细胞和肝癌细胞凋亡 ,凋亡率达5 0 %以上。中枢神经系统的肿瘤细胞对重组可溶性TRAIL不敏感。重组可溶性TRAIL在体内能显著抑制人非小细胞肺癌细胞在小鼠体内生长 ,抑制率达 70 %以上。结论为研究制备的重组可溶性TRAIL能在体内外杀死多种肿瘤细胞 ,具有显著的临床应用前景。 展开更多
关键词 重组可溶性trail 表达 生物学活性 肿瘤细胞凋亡
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腺病毒载体携带人TRAIL基因治疗H446小细胞肺癌的体外实验研究 被引量:9
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作者 刘永靖 陈飞虎 +3 位作者 于奇 韩继彪 苏长青 钱其军 《中国药理学通报》 CAS CSCD 北大核心 2007年第6期755-759,共5页
目的评价携带人TRAIL基因的腺病毒载体对人小细胞肺癌细胞株H446的基因治疗功效。方法构建的携带人TRAIL基因的腺病毒Ad-hTRAIL,感染人小细胞肺癌细胞株H446以及人正常肝细胞株WRL-68,人正常成纤维细胞株MRC-5,通过四甲基偶氮唑蓝(MTT)... 目的评价携带人TRAIL基因的腺病毒载体对人小细胞肺癌细胞株H446的基因治疗功效。方法构建的携带人TRAIL基因的腺病毒Ad-hTRAIL,感染人小细胞肺癌细胞株H446以及人正常肝细胞株WRL-68,人正常成纤维细胞株MRC-5,通过四甲基偶氮唑蓝(MTT)比色法检测其杀伤肿瘤细胞的效能,流式细胞术(FCM)检测Ad-hTRAIL对细胞早期凋亡的影响,并进行RT-PCR、酶联免疫吸附试验(ELISA)检测TRAIL mRNA和TRAIL蛋白的表达量。结果携带人TRAIL基因的增殖缺陷型腺病毒Ad-hTRAIL对H446细胞的感染效率较高;感染72h后H446、WRL-68、MRC-5细胞培养上清中TRAIL的表达量分别为32.02、17.08、16.89μg.L-1,在MOI=1.0时,Ad-hTRAIL即可引起H446细胞明显凋亡;而在MOI=100时对正常细胞WRL-68、MRC-5也没有明显杀伤作用。结论Ad-hTRAIL能够强烈诱导小细胞肺癌细胞H446凋亡而对正常肝细胞及成纤维细胞无明显抑制作用,Ad-hTRAIL在小细胞肺癌的基因治疗方面有潜在的应用前景。 展开更多
关键词 小细胞肺癌 基因治疗 腺病毒 trail 凋亡
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TRAIL蛋白的表达、纯化和抗肿瘤活性 被引量:14
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作者 李小安 房殿春 +1 位作者 杨仕明 罗元辉 《第三军医大学学报》 CAS CSCD 北大核心 2001年第9期1058-1060,共3页
目的 利用大肠杆菌表达出TRAIL蛋白 ,并观察分离纯化后的TRAIL蛋白的抗肿瘤活性。方法 采用CaCl2 法将带有TRAIL(氨基酸 114 -2 81,含六聚组氨酸尾 )基因的pET d质粒转入大肠杆菌BL2 1(DE3)pLysS ,IPTG诱导蛋白表达 ;使用Ni NTA层析... 目的 利用大肠杆菌表达出TRAIL蛋白 ,并观察分离纯化后的TRAIL蛋白的抗肿瘤活性。方法 采用CaCl2 法将带有TRAIL(氨基酸 114 -2 81,含六聚组氨酸尾 )基因的pET d质粒转入大肠杆菌BL2 1(DE3)pLysS ,IPTG诱导蛋白表达 ;使用Ni NTA层析柱分离纯化蛋白 ,SDS PAGE和Westernblot法鉴定TRAIL蛋白的表达 ;MTT法检测TRAIL蛋白的抗肿瘤活性。结果 我们分离纯化出分子量为 2 0 .1× 10 3 的蛋白 ,Westernblot法证实为TRAIL蛋白 ,MTT法检测出其有较高的抗肿瘤活性 ,呈现出较好的时效和量效关系。结论 成功地利用大肠杆菌表达、Ni NTA层析柱分离纯化出具有抗肿瘤活性的TRAIL蛋白 。 展开更多
关键词 trail蛋白 大肠杆菌 抗肿瘤活性 药理作用 抗癌药
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人参皂苷Rg_3与TRAIL联合应用对大肠癌细胞株HCE8693作用的实验研究 被引量:11
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作者 何超 吴彤 +2 位作者 胡晓彤 梁建华 陈萍 《中国药学杂志》 EI CAS CSCD 北大核心 2004年第8期599-601,616,共4页
目的将人参提取成分20(R)-人参皂苷Rg3和肿瘤坏死因子(TNF)相关的凋亡诱导配体(TNF-related apoptosis-inducing ligand,TRAIL)基因,联合应用于人大肠癌细胞株HCE8693,来寻求传统中药与现代基因治疗对肿瘤治疗的新途径。方法将人参皂苷... 目的将人参提取成分20(R)-人参皂苷Rg3和肿瘤坏死因子(TNF)相关的凋亡诱导配体(TNF-related apoptosis-inducing ligand,TRAIL)基因,联合应用于人大肠癌细胞株HCE8693,来寻求传统中药与现代基因治疗对肿瘤治疗的新途径。方法将人参皂苷Rg3联合重组腺病毒载体介导的TRAIL基因(Ad/GT-TRAIL)作用于大肠癌细胞株HCE8693。通过MTT比色法与流式细胞仪研究分析其对HCE8693细胞的作用效果。结果 单独应用浓度为0.01%,0.017%,0.025%,0.05%的人参皂苷Rg3及2000MOI的Ad/GT-TRAIL对HCE8693细胞的生长抑制率分别为8.6%,8.6%,18.7%,21.9%和24.3%;凋亡诱导率分别为5.4%,7.6%,20.3%,20.9%和23.2%。联合应用后,对HCE8693细胞株的生长抑制率及凋亡率均有显著的增强作用,分别达17.8%,25.7%,37.3%,46.2%及18.9%,20.1%,46.2%,56.0%。人参皂苷Rg3对HCE8693的生长抑制及促凋亡作用存在浓度依赖现象。结论一定浓度的人参皂苷Rg3或TRAIL能有效抑制HCE8693的生长,人参皂苷Rg3对HCE8693的生长抑制及促凋亡作用有浓度依赖性。联合应用后,其对HCE8693的生长抑制作用及凋亡诱导作用均明显增强,其协同作用的机制值得进一步探索。 展开更多
关键词 人参皂苷RG3 trail 大肠癌 HCE8693细胞 实验 凋亡诱导配体 肿瘤坏死因子
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肿瘤靶向腺相关病毒携带干扰素β和TRAIL对A549肺癌移植瘤的增强治疗效应(英文) 被引量:6
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作者 王毅刚 何凌峰 +5 位作者 何国清 孔彦平 刘旭平 蔡海波 刘新垣 谭文松 《生物工程学报》 CAS CSCD 北大核心 2010年第6期780-788,共9页
干扰素β(IFN-β)和肿瘤坏死因子相关凋亡诱导配体(TRAIL)是有效抗癌药物。腺相关病毒(AAV)为目前最有应用前景的基因转移载体之一。利用AAV携带IFN-β和TRAIL基因并置于hTERT启动子控制下分别构建成肿瘤靶向病毒AAV-hTERT-IFN-β和AAV-... 干扰素β(IFN-β)和肿瘤坏死因子相关凋亡诱导配体(TRAIL)是有效抗癌药物。腺相关病毒(AAV)为目前最有应用前景的基因转移载体之一。利用AAV携带IFN-β和TRAIL基因并置于hTERT启动子控制下分别构建成肿瘤靶向病毒AAV-hTERT-IFN-β和AAV-hTERT-TRAIL,且单个IFN-β或TRAIL基因治疗发挥了一定的抗癌效果。将AAV-hTERT-IFN-β和AAV-hTERT-TRAIL进行联合,旨在研究其对A549肺癌细胞体内外的生长抑制效应。ELISA法检测了AAV-hTERT-IFN-β感染A549细胞后分泌型IFN-β的表达;MTT法检测AAV-hTERT-IFN-β联合AAV-hTERT-TRAIL对肿瘤细胞的生长抑制作用;凋亡细胞染色和流式细胞仪分别检测了AAV-hTERT-IFN-β、AAV-hTERT-TRAIL及其联合对A549细胞的凋亡效应;进一步评价了联合AAV-hTERT-IFN-β和AAV-hTERT-TRAIL对A549裸鼠移植瘤的抑癌效果。结果显示,联合治疗优于任一单独治疗并且导致了增强的肿瘤细胞毒性和凋亡诱导效应。更进一步显示,联合AAV-hTERT-IFN-β和AAV-hTERT-TRAIL治疗发挥了重要的抑制裸鼠移植瘤效果甚至消除全部移植瘤,为探究IFN-β和TRAIL联合抗癌的分子机制奠定了基础。 展开更多
关键词 干扰素Β trail 腺相关病毒 肺癌 抗肿瘤效应
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肿瘤坏死因子相关的凋亡诱导配体(TRAIL)cDNA的克隆、表达和活性测定 被引量:7
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作者 侯登勇 颜真 +2 位作者 韩苇 赵宁 张英起 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2002年第1期27-31,共5页
肿瘤坏死因子相关的凋亡诱导配体 (TRAIL)能选择性诱导肿瘤细胞凋亡 .为利用基因工程技术获得重组TRAIL蛋白可溶性片段 (sTRAIL) ,设计 1对引物 .利用PCR技术特异性扩增出sTRAIL的cDNA ,克隆于质粒pGEM 3Zf( )的EcoRⅠ和PstⅠ位点 .经... 肿瘤坏死因子相关的凋亡诱导配体 (TRAIL)能选择性诱导肿瘤细胞凋亡 .为利用基因工程技术获得重组TRAIL蛋白可溶性片段 (sTRAIL) ,设计 1对引物 .利用PCR技术特异性扩增出sTRAIL的cDNA ,克隆于质粒pGEM 3Zf( )的EcoRⅠ和PstⅠ位点 .经测序证明序列正确后克隆于表达质粒pBV2 2 0的EcoRⅠ和PstⅠ位点 ,转化大肠杆菌DH5α .转化菌株经温度诱导 ,SDS PAGE检测和Western印迹鉴定 ,获得重组sTRAIL的高水平非融合表达菌株 .表达量占菌体总蛋白的 2 0 % .对其表达产物进行了初步纯化 ,SDS PAGE结果显示纯度可达 90 %以上 .用L92 9细胞测定其生物学活性表明 。 展开更多
关键词 trail 克隆 表达 诱导凋亡配体 TNF 肿瘤坏死因子 活性测定
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抑制JNK通路对TRAIL诱导胃癌细胞凋亡影响的研究 被引量:7
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作者 刘静 曲秀娟 +3 位作者 刘云鹏 徐玲 侯科佐 张晔 《中华肿瘤防治杂志》 CAS 2011年第7期481-484,共4页
目的:研究JNK信号传导通路对TRAIL诱导胃癌细胞凋亡的影响。方法:四甲基偶氮唑蓝(MTT)比色法测定细胞活力;蛋白质印迹法检测细胞内磷酸化JNK及总JNK的表达水平;流式细胞术碘化丙啶(PI)染色检测细胞凋亡及细胞周期分布。为研究JNK通路活... 目的:研究JNK信号传导通路对TRAIL诱导胃癌细胞凋亡的影响。方法:四甲基偶氮唑蓝(MTT)比色法测定细胞活力;蛋白质印迹法检测细胞内磷酸化JNK及总JNK的表达水平;流式细胞术碘化丙啶(PI)染色检测细胞凋亡及细胞周期分布。为研究JNK通路活性对TRAIL抗肿瘤作用的影响,将实验分为空白对照组、TRAIL单药组(100μg/L)、JNK抑制剂组(SP600125,20μmol/L)和联合用药组(SP600125+TRAIL)。结果:采用25、50、100和200μg/LTRAIL作用于MGC803细胞24 h,细胞活力仅轻度下降。进一步研究发现,TRAIL作用后细胞内磷酸化JNK水平明显升高,提示JNK信号通路被活化。同TRAIL单药组相比,联合用药组的细胞活力明显降低〔(53.5±3.2)%vs(88.3±1.1)%,P<0.05〕,细胞凋亡明显增加〔(21.3±5.1)%vs(5.7±0.1)%,P<0.05〕,G2/M期细胞比例明显升高〔(38.0±6.0)%vs(25.7±2.9)%,P<0.05〕。结论:抑制JNK通路能明显增强TRAIL对MGC803细胞的抗肿瘤作用,其机制可能与诱导细胞凋亡及G2/M期阻滞有关。 展开更多
关键词 胃肿瘤 JNK trail 细胞凋亡
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肾康丸对2型糖尿病大鼠肾脏TRAIL系统表达的影响 被引量:3
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作者 杨爱成 肖炜 +3 位作者 刘雅诗 魏连波 梁子安 马春成 《暨南大学学报(自然科学与医学版)》 CAS CSCD 北大核心 2013年第6期621-625,共5页
目的:观察肾康丸治疗对2型糖尿病(T2DM)大鼠肾组织TRAIL系统表达的影响,探讨其肾脏保护作用机制。方法:建立T2DM大鼠模型。成模后将大鼠随机分为2组:模型对照组(DM)、肾康丸组(DS);另设正常对照组(C)。分组干预,全自动生化仪检测高密度... 目的:观察肾康丸治疗对2型糖尿病(T2DM)大鼠肾组织TRAIL系统表达的影响,探讨其肾脏保护作用机制。方法:建立T2DM大鼠模型。成模后将大鼠随机分为2组:模型对照组(DM)、肾康丸组(DS);另设正常对照组(C)。分组干预,全自动生化仪检测高密度脂蛋白胆固醇(FBG)、总胆固醇(TC)及空腹血糖(FBG)(HDL-C);ELISA法检测尿微量白蛋白α1-MG(Uα1-MG)、24 h尿微量白蛋白(24h UmAlb),Q-RT-PCR法检测TRAIL、DR4、DcR2mRNA在肾组织表达,HE染色法观察大鼠肾组织病理变化。结果:随着实验时间的延长,DM组FBG及TC升高,HDL-C降低,而DS组FBG及TC降低,HDL-C升高;与C组相比,第4周、第8周DM组大鼠Uα1-MG、24h UmAlb均显著升高(P<0.01,P<0.01),肾组织DcR2 mRNA表达显著增强(P<0.01);而TRAIL、DR4 mRNA表达显著减弱(P<0.01),并且DM组大鼠出现了肾脏组织病理损害;DS组各指标均呈相反改变,肾脏病理损害减轻。结论:肾康丸通过影响TRAIL系统的表达,对T2DM大鼠具有显著的肾保护作用。 展开更多
关键词 肾康丸 2型糖尿病 糖尿病肾病 trail系统
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