The protection of aquatic ecosystems is of great importance to maintain the biological balance necessary to secure healthy foods therein imbedded. Toho Lake, located to the south east of Benin, is threatened by pollut...The protection of aquatic ecosystems is of great importance to maintain the biological balance necessary to secure healthy foods therein imbedded. Toho Lake, located to the south east of Benin, is threatened by pollution emanating from anthropogenic activities with the use of chemical fertilizers, effluent of domestic waste, particularly human and animal excrement with neighbourhood effluents. This study aimed at assessing some heavy metals in fresh fish (Oreochromis aureus) of Toho Lake in order to secure healthy food for the population and protect the ecosystem. Samples of water and fishes were collected and analyzed by HACH DR 3900 after treatment. Results revealed a mean oxygenation of water (4.95 mgo2/l), Biochemical Oxygen Demand (21.5 mgo2/l), Chemical oxygen Demand (149, 39 mg/l) with the nitrogen in Nitrate (NO3: 0.18 mg/l), ammonium nitrate (N-N: 0.47 mg/l), and phosphorous (2.06 mg/l) to be very high. The highest contents of heavy metals obtained in the fresh fish were cadmium (32.25 mg/kg), copper (115 mg/kg), lead (8.25 mg/kg), and zinc (90.75 mg/kg) and revealed that the fishes of Toho lake were polluted. The finding allowed us to conclude that the pollution of the lake and the fish carnage are due to anthropogenic pollution through chemicals spilling. Some protection methods of the lake and ecosystem are proposed in order to keep the fish safe and protect the well-being of the population.展开更多
Extended-spectrum <i>β</i>-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on ...Extended-spectrum <i>β</i>-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on the blaTEM, blaCTX-M and blaSHV genes in ESBL producing Enterobacteria, however, very few studies reported in the literature were related to blaCTX-M subgroup blaTOHO. TOHO enzymes were responsible for healthcare-associated infections in hospitals and in the community. In Burkina Faso, data related to these types of enzymes were scarce. The purpose of this study was to detect TOHO enzymes in <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> in order to know the prevalence of infections related to bacterial resistance due to TOHO enzymes at Saint Camille Hospital of Ouagadougou (Burkina Faso). The study was conducted firstly by microbiological identification of ESBLs-producing by <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> using API 20 E gallery;secondly the antibiogram was performed by the diffusion method and finally the molecular characterization was made by conventional PCR to search for the blaTOHO gene. The visualization of the specific bands was made using the ultraviolet lamp (Gene Flash) for the photography of the gels. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value < 0.05 was considered as significant. We obtained at all 39 strains constituted by 21 (53.8%) <i>Escherichia coli</i> and 18 (46.2%) <i>Klebsiella pneumoniae</i>. Molecular characterization showed the presence of the blaTOHO gene in 25 bacterial strains (64.1%). It was therefore established in this study the existence of blaTOHO gene at Saint Camille Hospital in Ouagadougou in Burkina Faso. Our study made it possible to know the distribution of the blaTOHO gene in <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i>.展开更多
文摘The protection of aquatic ecosystems is of great importance to maintain the biological balance necessary to secure healthy foods therein imbedded. Toho Lake, located to the south east of Benin, is threatened by pollution emanating from anthropogenic activities with the use of chemical fertilizers, effluent of domestic waste, particularly human and animal excrement with neighbourhood effluents. This study aimed at assessing some heavy metals in fresh fish (Oreochromis aureus) of Toho Lake in order to secure healthy food for the population and protect the ecosystem. Samples of water and fishes were collected and analyzed by HACH DR 3900 after treatment. Results revealed a mean oxygenation of water (4.95 mgo2/l), Biochemical Oxygen Demand (21.5 mgo2/l), Chemical oxygen Demand (149, 39 mg/l) with the nitrogen in Nitrate (NO3: 0.18 mg/l), ammonium nitrate (N-N: 0.47 mg/l), and phosphorous (2.06 mg/l) to be very high. The highest contents of heavy metals obtained in the fresh fish were cadmium (32.25 mg/kg), copper (115 mg/kg), lead (8.25 mg/kg), and zinc (90.75 mg/kg) and revealed that the fishes of Toho lake were polluted. The finding allowed us to conclude that the pollution of the lake and the fish carnage are due to anthropogenic pollution through chemicals spilling. Some protection methods of the lake and ecosystem are proposed in order to keep the fish safe and protect the well-being of the population.
文摘Extended-spectrum <i>β</i>-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on the blaTEM, blaCTX-M and blaSHV genes in ESBL producing Enterobacteria, however, very few studies reported in the literature were related to blaCTX-M subgroup blaTOHO. TOHO enzymes were responsible for healthcare-associated infections in hospitals and in the community. In Burkina Faso, data related to these types of enzymes were scarce. The purpose of this study was to detect TOHO enzymes in <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> in order to know the prevalence of infections related to bacterial resistance due to TOHO enzymes at Saint Camille Hospital of Ouagadougou (Burkina Faso). The study was conducted firstly by microbiological identification of ESBLs-producing by <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> using API 20 E gallery;secondly the antibiogram was performed by the diffusion method and finally the molecular characterization was made by conventional PCR to search for the blaTOHO gene. The visualization of the specific bands was made using the ultraviolet lamp (Gene Flash) for the photography of the gels. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value < 0.05 was considered as significant. We obtained at all 39 strains constituted by 21 (53.8%) <i>Escherichia coli</i> and 18 (46.2%) <i>Klebsiella pneumoniae</i>. Molecular characterization showed the presence of the blaTOHO gene in 25 bacterial strains (64.1%). It was therefore established in this study the existence of blaTOHO gene at Saint Camille Hospital in Ouagadougou in Burkina Faso. Our study made it possible to know the distribution of the blaTOHO gene in <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i>.
