C-terminal sequencing is important for characterized the primary structure of protein and polypeptides and it must do for quality control of recombination protein drugs.A method of MALDI-TOF-TOF-MS with in source deca...C-terminal sequencing is important for characterized the primary structure of protein and polypeptides and it must do for quality control of recombination protein drugs.A method of MALDI-TOF-TOF-MS with in source decay(ISD) to measure C-terminal sequence of protein was established.This method was applied successfully to measure C-terminal sequence of Neuregulin,TP-Ⅱ,ubiquitin,and myoglobin,respectively and 10,24,24 and 36 amino acid residue of C-terminal were measured.展开更多
目的初步建立冠心病患者与健康对照的血小板蛋白质表达图谱,并鉴定其差异表达的蛋白质。方法选取冠心病患者9例和健康志愿者11名作为研究对象,抽取外周血,分离血小板并提取蛋白质。采用二维凝胶电泳技术分离并建立血小板蛋白质表达图谱,...目的初步建立冠心病患者与健康对照的血小板蛋白质表达图谱,并鉴定其差异表达的蛋白质。方法选取冠心病患者9例和健康志愿者11名作为研究对象,抽取外周血,分离血小板并提取蛋白质。采用二维凝胶电泳技术分离并建立血小板蛋白质表达图谱,以Image Master 2D platinum 5.0软件进行图谱分析,选取差异点进行基质辅助激光解析/电离-飞行时间质谱(Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry,MALDI-TOF-TOF)分析,再采用Mascot软件搜索数据库鉴定差异表达蛋白质。结果 (1)初步建立了两组对象的血小板蛋白质表达图谱;(2)筛选出17个差异蛋白点,质谱成功鉴定7个:Plastin-2、Coronin-1A、Clusterin、Annexin A1、Chloride intracellular channel protein 1、Peroxiredoxin-6、Peroxiredoxin-2。结论血小板蛋白Plastin-2、Coronin-1A、Clusterin、Annexin A1、Peroxiredoxin-6、Peroxiredoxin-2在冠心病的发生发展中可能起到关键作用。展开更多
文摘C-terminal sequencing is important for characterized the primary structure of protein and polypeptides and it must do for quality control of recombination protein drugs.A method of MALDI-TOF-TOF-MS with in source decay(ISD) to measure C-terminal sequence of protein was established.This method was applied successfully to measure C-terminal sequence of Neuregulin,TP-Ⅱ,ubiquitin,and myoglobin,respectively and 10,24,24 and 36 amino acid residue of C-terminal were measured.
文摘目的初步建立冠心病患者与健康对照的血小板蛋白质表达图谱,并鉴定其差异表达的蛋白质。方法选取冠心病患者9例和健康志愿者11名作为研究对象,抽取外周血,分离血小板并提取蛋白质。采用二维凝胶电泳技术分离并建立血小板蛋白质表达图谱,以Image Master 2D platinum 5.0软件进行图谱分析,选取差异点进行基质辅助激光解析/电离-飞行时间质谱(Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry,MALDI-TOF-TOF)分析,再采用Mascot软件搜索数据库鉴定差异表达蛋白质。结果 (1)初步建立了两组对象的血小板蛋白质表达图谱;(2)筛选出17个差异蛋白点,质谱成功鉴定7个:Plastin-2、Coronin-1A、Clusterin、Annexin A1、Chloride intracellular channel protein 1、Peroxiredoxin-6、Peroxiredoxin-2。结论血小板蛋白Plastin-2、Coronin-1A、Clusterin、Annexin A1、Peroxiredoxin-6、Peroxiredoxin-2在冠心病的发生发展中可能起到关键作用。
