Articular cartilage has a limited self-healing capacity,leading to joint degeneration and osteoarthritis over time.Therefore,bioactive scaffolds are gaining attention as a promising approach to regenerating and repair...Articular cartilage has a limited self-healing capacity,leading to joint degeneration and osteoarthritis over time.Therefore,bioactive scaffolds are gaining attention as a promising approach to regenerating and repairing damaged articular cartilage through tissue engineering.In this study,we reported on a novel 3D bio-printed proteinaceous bioactive scaffolds combined with natural porcine cancellous bone dECM,tempo-oxidized cellulose nanofiber(TOCN),and alginate carriers for TGF-β1,FGF-18,and ADSCs to repair cartilage defects.The characterization results demonstrate that the 3D scaffolds are physically stable and facilitate a controlled dual release of TGF-β1 and FGF-18.Moreover,the key biological proteins within the bioactive scaffold actively interact with the biological systems to create a favorable microenvironment for cartilage regeneration.Importantly,the in vitro,in vivo,and in silico simulation showed that the scaffolds promote stem cell recruitment,migration,proliferation,and ECM deposition,and synergistic effects of TGF-β1/FGF-18 with the bioactive scaffolds significantly regulate stem cell chondrogenesis by activating the PI3K/AKT and TGFβ1/Smad4 signaling pathways.After implantation,the proteinaceous bioactive scaffold led to the regeneration of mechanically robust,full-thickness cartilage tissue that closely resembles native cartilage.Thus,these findings may provide a promising approach for regulating stem cell chondrogenesis and treating in situ cartilage regeneration.展开更多
Nanocellulose harvested from biomass has attractive properties that have promoted research on its practical applications.Herein,we investigated nanocellulose-based porous monoliths with oriented microchannels that can...Nanocellulose harvested from biomass has attractive properties that have promoted research on its practical applications.Herein,we investigated nanocellulose-based porous monoliths with oriented microchannels that can be fabricated via a unidirectional freezing method.In this method,water-dispersed cellulose nanofibers(CNFs)were immersed into a cold source at a controlled speed,followed by subsequent freeze-drying.The structure of porous cellulose monoliths mainly depends on two factors:the freezing conditions and properties of the dispersed CNFs.The former has been investigated previously.However,the effects of the latter remain unclear.In this study,CNF suspensions prepared by 2,2,6,6-tetramethylpiperidine-1-oxylmediated oxidation cellulose nanofibers(TOCNs)with different aspect ratios and concentrations were used.The effects of these variables on the resulting structure,including the pore shape,size,wall thickness,were examined.Based on the results,the impact of TOCNs on the structure of porous cellulose monoliths was investigated.Our findings suggested that depending on their structure,the porous cellulose monoliths exhibit different mechanical strengths and mass transport properties.In particular,porous cellulose monoliths synthesized from 5.1 wt.%short TOCNs exhibited a low density(55.9 mg∙cm^(−3)),high mechanical strength(8687 kPa),fast mass transport.展开更多
基金supported by the Basic Science Research Program through the National Research Foundation(NRF),funded by the Ministry of Education,Republic of Korea(2015R1A6A1A03032522)Technology Development Program(RS-2023-00281111)funded by the Ministry of SMEs and Startups(MSS,Korea)and partially funded by the Soonchunhyang University,Republic of Korea.
文摘Articular cartilage has a limited self-healing capacity,leading to joint degeneration and osteoarthritis over time.Therefore,bioactive scaffolds are gaining attention as a promising approach to regenerating and repairing damaged articular cartilage through tissue engineering.In this study,we reported on a novel 3D bio-printed proteinaceous bioactive scaffolds combined with natural porcine cancellous bone dECM,tempo-oxidized cellulose nanofiber(TOCN),and alginate carriers for TGF-β1,FGF-18,and ADSCs to repair cartilage defects.The characterization results demonstrate that the 3D scaffolds are physically stable and facilitate a controlled dual release of TGF-β1 and FGF-18.Moreover,the key biological proteins within the bioactive scaffold actively interact with the biological systems to create a favorable microenvironment for cartilage regeneration.Importantly,the in vitro,in vivo,and in silico simulation showed that the scaffolds promote stem cell recruitment,migration,proliferation,and ECM deposition,and synergistic effects of TGF-β1/FGF-18 with the bioactive scaffolds significantly regulate stem cell chondrogenesis by activating the PI3K/AKT and TGFβ1/Smad4 signaling pathways.After implantation,the proteinaceous bioactive scaffold led to the regeneration of mechanically robust,full-thickness cartilage tissue that closely resembles native cartilage.Thus,these findings may provide a promising approach for regulating stem cell chondrogenesis and treating in situ cartilage regeneration.
基金JSPS KAKENHI(No.22K18047)JST SICORP(No.JPMJSC2112)the New Energy and Industrial Technology Development Organization(NEDO)(No.JPNP20004)。
文摘Nanocellulose harvested from biomass has attractive properties that have promoted research on its practical applications.Herein,we investigated nanocellulose-based porous monoliths with oriented microchannels that can be fabricated via a unidirectional freezing method.In this method,water-dispersed cellulose nanofibers(CNFs)were immersed into a cold source at a controlled speed,followed by subsequent freeze-drying.The structure of porous cellulose monoliths mainly depends on two factors:the freezing conditions and properties of the dispersed CNFs.The former has been investigated previously.However,the effects of the latter remain unclear.In this study,CNF suspensions prepared by 2,2,6,6-tetramethylpiperidine-1-oxylmediated oxidation cellulose nanofibers(TOCNs)with different aspect ratios and concentrations were used.The effects of these variables on the resulting structure,including the pore shape,size,wall thickness,were examined.Based on the results,the impact of TOCNs on the structure of porous cellulose monoliths was investigated.Our findings suggested that depending on their structure,the porous cellulose monoliths exhibit different mechanical strengths and mass transport properties.In particular,porous cellulose monoliths synthesized from 5.1 wt.%short TOCNs exhibited a low density(55.9 mg∙cm^(−3)),high mechanical strength(8687 kPa),fast mass transport.
