本文于2025年07月发表于《Blood》杂志,发表后引起业内广泛关注,Blood发布述评文章(Thrombopoiesis comes full circle,Amelia A Rodolf,Owen J T McCarty,2025,10,23)。本文题录为:Wu H,Lu Y,Sun D,et al.circFUT8promotes proplatelet...本文于2025年07月发表于《Blood》杂志,发表后引起业内广泛关注,Blood发布述评文章(Thrombopoiesis comes full circle,Amelia A Rodolf,Owen J T McCarty,2025,10,23)。本文题录为:Wu H,Lu Y,Sun D,et al.circFUT8promotes proplatelet formation by interacting with IGF2BP2 and stabilizing TNS1 mRNA in megakaryocytes.Blood.2025;146(17):2119-2132.该研究首次揭示了环状RNA circFUT8促进血小板生成的重要作用,阐明了circFUT8通过与IGF2BP2相互作用促进巨核细胞中TNS1 mRNA的稳定,进而调控巨核细胞肌动蛋白骨架重排的作用机制。本研究揭示了血小板生成调控的新机制,拓宽了环形RNA的生物学功能,为血小板减少症提供了潜在的治疗靶点,也为血小板体外生成策略提供了新的理论依据。现在作者将再次通过佳文解读的方式来阐述这一研究。展开更多
目的:探讨miR-31-5p/张力蛋白1基因(tension protein 1,TNS1)分子轴对乳腺癌细胞生物学行为的影响及其放疗抵抗的分子机制。方法:收集2017年7月至2017年12月南阳市中心医院肿瘤放疗科收治的、经手术切除的21例乳腺癌患者癌及癌旁组织标...目的:探讨miR-31-5p/张力蛋白1基因(tension protein 1,TNS1)分子轴对乳腺癌细胞生物学行为的影响及其放疗抵抗的分子机制。方法:收集2017年7月至2017年12月南阳市中心医院肿瘤放疗科收治的、经手术切除的21例乳腺癌患者癌及癌旁组织标本,以及乳腺癌细胞系MCF-7、MDA-MB-231和SKBR-3,采用qPCR法检测癌组织和癌细胞系中miR-31-5p的表达水平。通过6 MV-X射线照射MCF-7细胞,构建放疗抵抗细胞株MCF-7R。随后,采用克隆形成实验、Transwell小室法和Annexin V/PI染色流式细胞术检测过表达/敲降miR-31-5p对MCF-7和MCF-7R细胞放疗敏感性的影响;用双荧光素酶报告基因验证miR-31-5p与TNS1的靶向关系。结果:乳腺癌组织、细胞系和MCF-7R细胞中miR-31-5p表达水平显著低于癌旁组织、人正常乳腺上皮细胞MCF-10A和MCF-7细胞(均P<0.01)。过表达miR-31-5p显著抑制MCF-7R细胞的侵袭并促进细胞凋亡(均P<0.01),沉默miR-31-5p在MCF-7细胞中结果相反。双荧光素酶报告基因法证实TNS1是miR-31-5p靶基因。过表达miR-31-5p通过靶向下调TNS1显著抑制MCF-7R细胞侵袭能力并促进细胞凋亡(均P<0.01),沉默miR-31-5p通过上调TNS1显著促进MCF-7细胞侵袭和抑制细胞凋亡(均P<0.01),从而上调MCF-7R对放射治疗的敏感性。结论:miR-31-5p/TNS1分子轴与乳腺癌放疗抵抗存在调控关系,且过表达miR-31-5p可逆转MCF-7R细胞对放疗抵抗作用。展开更多
pHNC3, a suicide plasmid encoding luxAB and scaB genes in Tn5,can be transfered into Rhizobium through tri-parental mating and thenthe Rhizobium is labelled by luxAB. Since the levansucrasc activitycoded by sacB gene ...pHNC3, a suicide plasmid encoding luxAB and scaB genes in Tn5,can be transfered into Rhizobium through tri-parental mating and thenthe Rhizobium is labelled by luxAB. Since the levansucrasc activitycoded by sacB gene can cause the sucrose sensitivity on Gram-negativebacteria,the sacB gene allowed direct selection for the deletion or curingof plasmide, because only strains which no longer contain an activesacB gene are able to grow on media containing sucrose. This negativeselecting model can be used for direct labelling the mega-plasmid ofRhizobium,or even getting Rhizobium deriyatives partial deleted or curedtheir mega-plasmids.展开更多
Organic green light emitting devices(LEDs) with multi-quantum well(MQW) structure were fabricated. Aromatic diamine(TPD) was used as hole-transporting layer and potential barrier layer; Tris(8-hydroxyquinoline) alumin...Organic green light emitting devices(LEDs) with multi-quantum well(MQW) structure were fabricated. Aromatic diamine(TPD) was used as hole-transporting layer and potential barrier layer; Tris(8-hydroxyquinoline) aluminum(Alq 3) was acted as electron-transporting emitter and MQW green emitter. Air-stable aluminum(Al) was used as electron-injection contact. The influence of the thickness of potential barrier layer and the number of quantum well on the electroluminescent(EL) efficiencies of the devices was investigated. The organic LEDs with two quantum wells showed enhanced EL efficiencies. Maximum external quantum efficiency and brightness were 1.04 % and 7 000 cd/m 2, respectively.展开更多
Age-related macular degeneration(AMD)poses a significant threat to the vision of the elderly population globally.Unfortunately,there is no effective treatment available for dry AMD.In this study,we utilized human reti...Age-related macular degeneration(AMD)poses a significant threat to the vision of the elderly population globally.Unfortunately,there is no effective treatment available for dry AMD.In this study,we utilized human retinal organoids(ROs)stimulated with sodium iodate to establish a model for dry AMD.We assessed the apoptosis of retinal organoid cells and conducted RNA sequencing to analyze molecular changes.Our findings indicate that metformin and the fetal hemoglobin(HbF)inducer TN1 could protect ROs from sodium iodate induced damage and restore retinal function in murine model.The administration of metformin and TN1 alleviated apoptosis in ROs and improved visual function.Studies of molecular mechanisms indicated that the protective effects of metformin and TN1 may be related to the HMOX1 gene,providing valuable insights for the development of new therapies for dry AMD via targeting HMOX1 and its downstream pathways.展开更多
文摘本文于2025年07月发表于《Blood》杂志,发表后引起业内广泛关注,Blood发布述评文章(Thrombopoiesis comes full circle,Amelia A Rodolf,Owen J T McCarty,2025,10,23)。本文题录为:Wu H,Lu Y,Sun D,et al.circFUT8promotes proplatelet formation by interacting with IGF2BP2 and stabilizing TNS1 mRNA in megakaryocytes.Blood.2025;146(17):2119-2132.该研究首次揭示了环状RNA circFUT8促进血小板生成的重要作用,阐明了circFUT8通过与IGF2BP2相互作用促进巨核细胞中TNS1 mRNA的稳定,进而调控巨核细胞肌动蛋白骨架重排的作用机制。本研究揭示了血小板生成调控的新机制,拓宽了环形RNA的生物学功能,为血小板减少症提供了潜在的治疗靶点,也为血小板体外生成策略提供了新的理论依据。现在作者将再次通过佳文解读的方式来阐述这一研究。
文摘目的:探讨miR-31-5p/张力蛋白1基因(tension protein 1,TNS1)分子轴对乳腺癌细胞生物学行为的影响及其放疗抵抗的分子机制。方法:收集2017年7月至2017年12月南阳市中心医院肿瘤放疗科收治的、经手术切除的21例乳腺癌患者癌及癌旁组织标本,以及乳腺癌细胞系MCF-7、MDA-MB-231和SKBR-3,采用qPCR法检测癌组织和癌细胞系中miR-31-5p的表达水平。通过6 MV-X射线照射MCF-7细胞,构建放疗抵抗细胞株MCF-7R。随后,采用克隆形成实验、Transwell小室法和Annexin V/PI染色流式细胞术检测过表达/敲降miR-31-5p对MCF-7和MCF-7R细胞放疗敏感性的影响;用双荧光素酶报告基因验证miR-31-5p与TNS1的靶向关系。结果:乳腺癌组织、细胞系和MCF-7R细胞中miR-31-5p表达水平显著低于癌旁组织、人正常乳腺上皮细胞MCF-10A和MCF-7细胞(均P<0.01)。过表达miR-31-5p显著抑制MCF-7R细胞的侵袭并促进细胞凋亡(均P<0.01),沉默miR-31-5p在MCF-7细胞中结果相反。双荧光素酶报告基因法证实TNS1是miR-31-5p靶基因。过表达miR-31-5p通过靶向下调TNS1显著抑制MCF-7R细胞侵袭能力并促进细胞凋亡(均P<0.01),沉默miR-31-5p通过上调TNS1显著促进MCF-7细胞侵袭和抑制细胞凋亡(均P<0.01),从而上调MCF-7R对放射治疗的敏感性。结论:miR-31-5p/TNS1分子轴与乳腺癌放疗抵抗存在调控关系,且过表达miR-31-5p可逆转MCF-7R细胞对放疗抵抗作用。
文摘pHNC3, a suicide plasmid encoding luxAB and scaB genes in Tn5,can be transfered into Rhizobium through tri-parental mating and thenthe Rhizobium is labelled by luxAB. Since the levansucrasc activitycoded by sacB gene can cause the sucrose sensitivity on Gram-negativebacteria,the sacB gene allowed direct selection for the deletion or curingof plasmide, because only strains which no longer contain an activesacB gene are able to grow on media containing sucrose. This negativeselecting model can be used for direct labelling the mega-plasmid ofRhizobium,or even getting Rhizobium deriyatives partial deleted or curedtheir mega-plasmids.
文摘Organic green light emitting devices(LEDs) with multi-quantum well(MQW) structure were fabricated. Aromatic diamine(TPD) was used as hole-transporting layer and potential barrier layer; Tris(8-hydroxyquinoline) aluminum(Alq 3) was acted as electron-transporting emitter and MQW green emitter. Air-stable aluminum(Al) was used as electron-injection contact. The influence of the thickness of potential barrier layer and the number of quantum well on the electroluminescent(EL) efficiencies of the devices was investigated. The organic LEDs with two quantum wells showed enhanced EL efficiencies. Maximum external quantum efficiency and brightness were 1.04 % and 7 000 cd/m 2, respectively.
基金supported by the Beijing Natural Science Foundation(7242176 to S.W.)the Third Medical Center of Chinese PLA Research Fund(2024BJ-07 to S.W.).
文摘Age-related macular degeneration(AMD)poses a significant threat to the vision of the elderly population globally.Unfortunately,there is no effective treatment available for dry AMD.In this study,we utilized human retinal organoids(ROs)stimulated with sodium iodate to establish a model for dry AMD.We assessed the apoptosis of retinal organoid cells and conducted RNA sequencing to analyze molecular changes.Our findings indicate that metformin and the fetal hemoglobin(HbF)inducer TN1 could protect ROs from sodium iodate induced damage and restore retinal function in murine model.The administration of metformin and TN1 alleviated apoptosis in ROs and improved visual function.Studies of molecular mechanisms indicated that the protective effects of metformin and TN1 may be related to the HMOX1 gene,providing valuable insights for the development of new therapies for dry AMD via targeting HMOX1 and its downstream pathways.
