为了探究鸡肿瘤坏死因子α诱导蛋白8样1(TNF alpha induced protein 8 like 1,TNFAIP8L1)基因序列特征,以及叶酸(FA)和甲氨蝶呤(MTX)对TNFAIP8L1基因在鸡胚肝脏组织不同发育阶段表达的影响,试验将孵化至第7天的240枚琅琊鸡种蛋分为生理...为了探究鸡肿瘤坏死因子α诱导蛋白8样1(TNF alpha induced protein 8 like 1,TNFAIP8L1)基因序列特征,以及叶酸(FA)和甲氨蝶呤(MTX)对TNFAIP8L1基因在鸡胚肝脏组织不同发育阶段表达的影响,试验将孵化至第7天的240枚琅琊鸡种蛋分为生理盐水组(注射0.1 mL生理盐水)、FA组[注射0.1 mL FA溶液(90μg FA溶于0.1 mL生理盐水中)]、MTX组[注射0.1 mL MTX溶液(5μg MTX溶于0.1 mL生理盐水中)]、FA和MTX组[注射0.1 mL FA+MTX混合液(90μg FA+5μg MTX溶于0.1 mL生理盐水中)],每组4个重复,每个重复15枚,利用PCR技术克隆鸡TNFAIP8L1基因开放阅读框(ORF),并对其进行生物信息学分析,同时利用荧光定量PCR方法分析FA和MTX对TNFAIP8L1基因在鸡胚孵化至第10天(10E)、第13天(13E)、第16天(16E)、第19天(19E)和出壳第1天(1D)肝脏中的表达差异。结果表明:克隆获得琅琊鸡TNFAIP8L1基因序列长726 bp,其中ORF区序列长561 bp,编码186个氨基酸。氨基酸多序列比对显示琅琊鸡与绿头鸭的氨基酸序列一致性最高(为97.85%),与马的一致性最低(为64.52%)。鸡TNFAIP8L1蛋白是一种亲水稳定蛋白;二级结构预测以α-螺旋(为72.04%)和无规则卷曲(为23.66%)为主要结构,三级结构与5jxd.1模板相似,其序列同源性为62.37%。10E和16E时,FA组和MTX组TNFAIP8L1基因相对表达量均显著低于生理盐水组(P<0.05);19E和1D时,FA组和MTX组TNFAIP8L1基因相对表达量与生理盐水组差异不显著(P>0.05)。说明在鸡胚发育前期FA和MTX可能对TNFAIP8L1基因表达有抑制作用。展开更多
AIM:To explore the effect of miR-184 and miR-205 on the proliferation and metastasis of conjunctival mucosa associated lymphoid tissue(MALT)lymphoma.METHODS:Tissue of tumor and adjacent normal control from 5 patients ...AIM:To explore the effect of miR-184 and miR-205 on the proliferation and metastasis of conjunctival mucosa associated lymphoid tissue(MALT)lymphoma.METHODS:Tissue of tumor and adjacent normal control from 5 patients with conjunctival MALT was included.RPMI8226 cell line was selected to verify the effect of mi RNAs in B cells.The function of micro RNA on the RPMI8226 cell apoptosis,migration and invasion was evaluated by apoptosis assay and Transwell assay.The m RNA and protein expression were examined by quantitative RT-PCR and Western blotting.The effect of micro RNA on regulation of downstream gene expression was evaluated by luciferase report assay.RESULTS:A decreased level of miR-184 and miR-205 was observed in MALT lymphoma tissue.Exogenous miR-184 and miR-205 analogues promoted apoptosis,and inhibited the survival,migration,and invasion of RPMI8226 cells.miR-184 and miR-205 inhibitor reversed the process.The RNA and protein level of Ras L10 B and TNFAIP8 were downregulated in MALT lymphoma tissue.The exogenous of miR-184 and miR-205 promoted the expression of Ras L10 B and TNFAIP8.Meanwhile,inhibition of miR-184 and miR-205 repressed the expression of target gene,Ras L10 B and TNFAIP8.CONCLUSION:miR-184 and miR-205 suppresses the tumorigenesis of conjunctival MALT lymphoma through regulating Ras L10 B and TNFAIP8.展开更多
肿瘤坏死因子α诱导蛋白-8[Tumor necrosis factor(TNF)alpha-induced protein 8,TNFAIP8/TIPE]家族是新近发现的与免疫调节及肿瘤生长密切相关的家族蛋白,是目前唯一已知的、脂类第二信使分子磷脂酰肌醇二磷酸(PIP2)和磷脂酰肌醇三磷酸...肿瘤坏死因子α诱导蛋白-8[Tumor necrosis factor(TNF)alpha-induced protein 8,TNFAIP8/TIPE]家族是新近发现的与免疫调节及肿瘤生长密切相关的家族蛋白,是目前唯一已知的、脂类第二信使分子磷脂酰肌醇二磷酸(PIP2)和磷脂酰肌醇三磷酸(PIP3)的转运蛋白。该家族包含四个成员:TNFAIP8/TIPE、TNFAIP8L1/TIPE1、TNFAIP8L2/TIPE2和TNFAIP8L3/TIPE3。先前的研究证明该家族的蛋白可以作为调节因子在肿瘤、炎症和细胞死亡中发挥重要作用,但越来越多的证据表明该家族蛋白还参与调节免疫,并作为第二信使参与自噬的发生。本文就近年来TIPE家族蛋白在肿瘤进展、炎症疾病的作用研究进展作一综述。展开更多
TIPE(Tumor necrosis factor-alpha-induced protein 8-like)家族是新近报道的免疫和肿瘤调节因子。该家族拥有四个高度同源的成员:TNFAIP8(Tumor necrosis factor-α-induced protein 8),TIPE1(TNFAIP8L1),TIPE2(TNFAIP8L2)和TIPE3(TNF...TIPE(Tumor necrosis factor-alpha-induced protein 8-like)家族是新近报道的免疫和肿瘤调节因子。该家族拥有四个高度同源的成员:TNFAIP8(Tumor necrosis factor-α-induced protein 8),TIPE1(TNFAIP8L1),TIPE2(TNFAIP8L2)和TIPE3(TNFAIP8L3)。它们虽然结构相似,但在组织、器官上的表达不同,在生物学功能及疾病中的作用存在较大的差别。TNFAIP8具有抑制细菌感染与促肿瘤迁移的作用;TIPE2是一种免疫和炎症负调控因子,可抑制某些肿瘤的生长;TIPE1可诱导细胞凋亡具有抑瘤效应;TIPE3可特异性结合磷脂第二信使,促进肿瘤生成。随着研究的深入,TIPE家族在多种疾病的发生发展过程中表现出重要的调控作用,然而其具体生物活性与分子机制有待进一步的研究。展开更多
The TIPE （tumor necrosis factor-a-induced protein 8-like） family are newly described regulators of immunity and tumorigenesis consisting of four highly homologous mammalian proteins： TNFAIP8 （tumor necrosis factor...The TIPE （tumor necrosis factor-a-induced protein 8-like） family are newly described regulators of immunity and tumorigenesis consisting of four highly homologous mammalian proteins： TNFAIP8 （tumor necrosis factor-a-induced protein 8）, TIPE1 （TNFAIP8-1ike 1, or TNFAIP8L1）, TIPE2 （TNFAIP8L2） and TIPE3 （TNFAIP8L3）. They are the only known transfer proteins of the lipid secondary messengers PIP2 （phosphatidylinositol 4,5-bisphosphate） and PIP3 （phosphatidylinositol 3,4,5-trisphosphate）. Cell-surface receptors, such as G-protein-coupled receptors and receptor tyrosine kinases, regulate inflammation and cancer via several signaling pathways, including the nuclear factor （NF）-KB and phosphoinositide-3 kinase （PI3K） pathways, the latter of which is upstream of both Akt and STAT3 activation. An expression analysis in humans demonstrated that the TIPE family is dysregulated in cancer and inflammation, and studies both in mice and in vitro have demonstrated that this family of proteins plays a critical role in tumorigenesis and inflammatory responses. In this review, we summarize the current literature for all four family members, with a special focus on the phenotypic manifestations present in the various knockout murine strains, as well as the related cell signalinR that has been elucidated to date.展开更多
文摘为了探究鸡肿瘤坏死因子α诱导蛋白8样1(TNF alpha induced protein 8 like 1,TNFAIP8L1)基因序列特征,以及叶酸(FA)和甲氨蝶呤(MTX)对TNFAIP8L1基因在鸡胚肝脏组织不同发育阶段表达的影响,试验将孵化至第7天的240枚琅琊鸡种蛋分为生理盐水组(注射0.1 mL生理盐水)、FA组[注射0.1 mL FA溶液(90μg FA溶于0.1 mL生理盐水中)]、MTX组[注射0.1 mL MTX溶液(5μg MTX溶于0.1 mL生理盐水中)]、FA和MTX组[注射0.1 mL FA+MTX混合液(90μg FA+5μg MTX溶于0.1 mL生理盐水中)],每组4个重复,每个重复15枚,利用PCR技术克隆鸡TNFAIP8L1基因开放阅读框(ORF),并对其进行生物信息学分析,同时利用荧光定量PCR方法分析FA和MTX对TNFAIP8L1基因在鸡胚孵化至第10天(10E)、第13天(13E)、第16天(16E)、第19天(19E)和出壳第1天(1D)肝脏中的表达差异。结果表明:克隆获得琅琊鸡TNFAIP8L1基因序列长726 bp,其中ORF区序列长561 bp,编码186个氨基酸。氨基酸多序列比对显示琅琊鸡与绿头鸭的氨基酸序列一致性最高(为97.85%),与马的一致性最低(为64.52%)。鸡TNFAIP8L1蛋白是一种亲水稳定蛋白;二级结构预测以α-螺旋(为72.04%)和无规则卷曲(为23.66%)为主要结构,三级结构与5jxd.1模板相似,其序列同源性为62.37%。10E和16E时,FA组和MTX组TNFAIP8L1基因相对表达量均显著低于生理盐水组(P<0.05);19E和1D时,FA组和MTX组TNFAIP8L1基因相对表达量与生理盐水组差异不显著(P>0.05)。说明在鸡胚发育前期FA和MTX可能对TNFAIP8L1基因表达有抑制作用。
文摘AIM:To explore the effect of miR-184 and miR-205 on the proliferation and metastasis of conjunctival mucosa associated lymphoid tissue(MALT)lymphoma.METHODS:Tissue of tumor and adjacent normal control from 5 patients with conjunctival MALT was included.RPMI8226 cell line was selected to verify the effect of mi RNAs in B cells.The function of micro RNA on the RPMI8226 cell apoptosis,migration and invasion was evaluated by apoptosis assay and Transwell assay.The m RNA and protein expression were examined by quantitative RT-PCR and Western blotting.The effect of micro RNA on regulation of downstream gene expression was evaluated by luciferase report assay.RESULTS:A decreased level of miR-184 and miR-205 was observed in MALT lymphoma tissue.Exogenous miR-184 and miR-205 analogues promoted apoptosis,and inhibited the survival,migration,and invasion of RPMI8226 cells.miR-184 and miR-205 inhibitor reversed the process.The RNA and protein level of Ras L10 B and TNFAIP8 were downregulated in MALT lymphoma tissue.The exogenous of miR-184 and miR-205 promoted the expression of Ras L10 B and TNFAIP8.Meanwhile,inhibition of miR-184 and miR-205 repressed the expression of target gene,Ras L10 B and TNFAIP8.CONCLUSION:miR-184 and miR-205 suppresses the tumorigenesis of conjunctival MALT lymphoma through regulating Ras L10 B and TNFAIP8.
文摘肿瘤坏死因子α诱导蛋白-8[Tumor necrosis factor(TNF)alpha-induced protein 8,TNFAIP8/TIPE]家族是新近发现的与免疫调节及肿瘤生长密切相关的家族蛋白,是目前唯一已知的、脂类第二信使分子磷脂酰肌醇二磷酸(PIP2)和磷脂酰肌醇三磷酸(PIP3)的转运蛋白。该家族包含四个成员:TNFAIP8/TIPE、TNFAIP8L1/TIPE1、TNFAIP8L2/TIPE2和TNFAIP8L3/TIPE3。先前的研究证明该家族的蛋白可以作为调节因子在肿瘤、炎症和细胞死亡中发挥重要作用,但越来越多的证据表明该家族蛋白还参与调节免疫,并作为第二信使参与自噬的发生。本文就近年来TIPE家族蛋白在肿瘤进展、炎症疾病的作用研究进展作一综述。
文摘TIPE(Tumor necrosis factor-alpha-induced protein 8-like)家族是新近报道的免疫和肿瘤调节因子。该家族拥有四个高度同源的成员:TNFAIP8(Tumor necrosis factor-α-induced protein 8),TIPE1(TNFAIP8L1),TIPE2(TNFAIP8L2)和TIPE3(TNFAIP8L3)。它们虽然结构相似,但在组织、器官上的表达不同,在生物学功能及疾病中的作用存在较大的差别。TNFAIP8具有抑制细菌感染与促肿瘤迁移的作用;TIPE2是一种免疫和炎症负调控因子,可抑制某些肿瘤的生长;TIPE1可诱导细胞凋亡具有抑瘤效应;TIPE3可特异性结合磷脂第二信使,促进肿瘤生成。随着研究的深入,TIPE家族在多种疾病的发生发展过程中表现出重要的调控作用,然而其具体生物活性与分子机制有待进一步的研究。
文摘The TIPE （tumor necrosis factor-a-induced protein 8-like） family are newly described regulators of immunity and tumorigenesis consisting of four highly homologous mammalian proteins： TNFAIP8 （tumor necrosis factor-a-induced protein 8）, TIPE1 （TNFAIP8-1ike 1, or TNFAIP8L1）, TIPE2 （TNFAIP8L2） and TIPE3 （TNFAIP8L3）. They are the only known transfer proteins of the lipid secondary messengers PIP2 （phosphatidylinositol 4,5-bisphosphate） and PIP3 （phosphatidylinositol 3,4,5-trisphosphate）. Cell-surface receptors, such as G-protein-coupled receptors and receptor tyrosine kinases, regulate inflammation and cancer via several signaling pathways, including the nuclear factor （NF）-KB and phosphoinositide-3 kinase （PI3K） pathways, the latter of which is upstream of both Akt and STAT3 activation. An expression analysis in humans demonstrated that the TIPE family is dysregulated in cancer and inflammation, and studies both in mice and in vitro have demonstrated that this family of proteins plays a critical role in tumorigenesis and inflammatory responses. In this review, we summarize the current literature for all four family members, with a special focus on the phenotypic manifestations present in the various knockout murine strains, as well as the related cell signalinR that has been elucidated to date.
