为了探究鸡肿瘤坏死因子α诱导蛋白8样1(TNF alpha induced protein 8 like 1,TNFAIP8L1)基因序列特征,以及叶酸(FA)和甲氨蝶呤(MTX)对TNFAIP8L1基因在鸡胚肝脏组织不同发育阶段表达的影响,试验将孵化至第7天的240枚琅琊鸡种蛋分为生理...为了探究鸡肿瘤坏死因子α诱导蛋白8样1(TNF alpha induced protein 8 like 1,TNFAIP8L1)基因序列特征,以及叶酸(FA)和甲氨蝶呤(MTX)对TNFAIP8L1基因在鸡胚肝脏组织不同发育阶段表达的影响,试验将孵化至第7天的240枚琅琊鸡种蛋分为生理盐水组(注射0.1 mL生理盐水)、FA组[注射0.1 mL FA溶液(90μg FA溶于0.1 mL生理盐水中)]、MTX组[注射0.1 mL MTX溶液(5μg MTX溶于0.1 mL生理盐水中)]、FA和MTX组[注射0.1 mL FA+MTX混合液(90μg FA+5μg MTX溶于0.1 mL生理盐水中)],每组4个重复,每个重复15枚,利用PCR技术克隆鸡TNFAIP8L1基因开放阅读框(ORF),并对其进行生物信息学分析,同时利用荧光定量PCR方法分析FA和MTX对TNFAIP8L1基因在鸡胚孵化至第10天(10E)、第13天(13E)、第16天(16E)、第19天(19E)和出壳第1天(1D)肝脏中的表达差异。结果表明:克隆获得琅琊鸡TNFAIP8L1基因序列长726 bp,其中ORF区序列长561 bp,编码186个氨基酸。氨基酸多序列比对显示琅琊鸡与绿头鸭的氨基酸序列一致性最高(为97.85%),与马的一致性最低(为64.52%)。鸡TNFAIP8L1蛋白是一种亲水稳定蛋白;二级结构预测以α-螺旋(为72.04%)和无规则卷曲(为23.66%)为主要结构,三级结构与5jxd.1模板相似,其序列同源性为62.37%。10E和16E时,FA组和MTX组TNFAIP8L1基因相对表达量均显著低于生理盐水组(P<0.05);19E和1D时,FA组和MTX组TNFAIP8L1基因相对表达量与生理盐水组差异不显著(P>0.05)。说明在鸡胚发育前期FA和MTX可能对TNFAIP8L1基因表达有抑制作用。展开更多
Noise-induced hearing loss is a worldwide public health issue that is characterized by temporary or permanent changes in hearing sensitivity.This condition is closely linked to inflammatory responses,and interventions...Noise-induced hearing loss is a worldwide public health issue that is characterized by temporary or permanent changes in hearing sensitivity.This condition is closely linked to inflammatory responses,and interventions targeting the inflammatory gene tumor necrosis factoralpha(TNFα)are known to mitigate cochlear noise damage.TNFα-induced proteins(TNFAIPs)are a family of translucent acidic proteins,and TNFAIP6 has a notable association with inflammatory responses.To date,there have been few reports on TNFAIP6 levels in the inner ear.To elucidate the precise mechanism,we generated transgenic mouse models with conditional knockout of Tnfaip6(Tnfaip6 cKO).Evaluation of hair cell morphology and function revealed no significant differences in hair cell numbers or ribbon synapses between Tnfaip6 cKO and wild-type mice.Moreover,there were no notable variations in hair cell numbers or hearing function in noisy environments.Our results indicate that Tnfaip6 does not have a substantial impact on the auditory system.展开更多
Background:There is growing evidence that the gene named tumor necrosis factorα–induced protein 6(TNFAIP6)has an important role in various tumors.However,a systematic pan-cancer analysis of TNFAIP6 is lacking.Here w...Background:There is growing evidence that the gene named tumor necrosis factorα–induced protein 6(TNFAIP6)has an important role in various tumors.However,a systematic pan-cancer analysis of TNFAIP6 is lacking.Here we aimed to analyze the expression of TNFAIP6 across multiple cancers and verify its expression during the progression of colon cancer.Methods:We performed a comprehensive bioinformatics analysis to examine the expression of TNFAIP6 across 27 tumor types.GEPIA2 was used to evaluate the effect of TNFAIP6 on clinical cancer prognosis.cBioportal was used to assess TNFAIP6 mutations.The correlation between TNFAIP6 and cancer immune infiltrates was explored using TIMER2.0.The CancerSEA database was used to perform functional analysis of TNFAIP6.Metascape was used to identify TNFAIP6-related gene enrichment pathways.Immunohistochemistry was performed to detect TNFAIP6 protein expression in the colon cancer.In addition,the Comparative Toxicogenomics Database was searched for known and possible antitumor drugs that may be associated with TNFAIP6.Results:We found that,in most of the cancers included in this analysis,TNFAIP6 was highly expressed,and there is a distinct relationship between TNFAIP6 expression and cancer prognosis.TNFAIP6 expression is associated with cancer-associated fibroblasts,neutrophils,and endothelial cells.TNFAIP6 and similar genes may also be involved in the PID_VEGF_VEGFR_pathway.Immunohistochemistry revealed an increasing trend of TNFAIP6 protein expression in normal,adenoma,and colon cancer tissues.Several known and possible antitumor drugs that may be associated with TNFAIP6 were identified in the Comparative Toxicogenomics Database.These results suggest that a number of drugsmay target TNFAIP6 during cancer treatment,including cisplatin,irinotecan,resveratrol,U 0126,NSC689534,genistein,NSC668394,oxaliplatin,plerixafor,topotecan,vincristine,flutamide,doxorubicin,MRK 003,folic acid,demecolcine,tunicamycin,zoledronic acid,and schizandrin B.Conclusions:TNFAIP6 may function as an oncogene in certain cancers.Furthermore,this study provides evidence that TNFAIP6 is an important factor in colon cancer progression.展开更多
目的研究肿瘤坏死因子α诱导蛋白3(tumor necrosis factorα-induced protein 3,TNFAIP3)在食管鳞癌中表达的临床病理意义及其对鳞癌预后的判断价值。方法免疫组织化学法检测100例随访食管鳞癌及80例癌旁组织中TNFAIP3蛋白的表达,并分...目的研究肿瘤坏死因子α诱导蛋白3(tumor necrosis factorα-induced protein 3,TNFAIP3)在食管鳞癌中表达的临床病理意义及其对鳞癌预后的判断价值。方法免疫组织化学法检测100例随访食管鳞癌及80例癌旁组织中TNFAIP3蛋白的表达,并分析了其与食管鳞癌临床病理特征及预后的关系。结果TNFAIP3在食管鳞癌中高表达阳性率为47.0%(47/100),显著高于癌旁组织中的高表达率15.0%(12/80),且与肿瘤的分化程度有关;生存分析显示高表达TNFAIP3的患者预后不良,单因素生存分析显示TNFAIP3的表达、肿瘤的浸润深度、淋巴结转移、临床分期与食管鳞癌预后密切相关,多因素生存分析显示TNFAIP3的表达、肿瘤的浸润深度、淋巴结转移、临床分期为食管鳞癌的独立预后预测因子。结论表达增高的TNFAIP3可能参与了食管鳞癌的发生发展,TNFAIP3的高表达可能成为食管鳞癌的独立预后因子。展开更多
文摘为了探究鸡肿瘤坏死因子α诱导蛋白8样1(TNF alpha induced protein 8 like 1,TNFAIP8L1)基因序列特征,以及叶酸(FA)和甲氨蝶呤(MTX)对TNFAIP8L1基因在鸡胚肝脏组织不同发育阶段表达的影响,试验将孵化至第7天的240枚琅琊鸡种蛋分为生理盐水组(注射0.1 mL生理盐水)、FA组[注射0.1 mL FA溶液(90μg FA溶于0.1 mL生理盐水中)]、MTX组[注射0.1 mL MTX溶液(5μg MTX溶于0.1 mL生理盐水中)]、FA和MTX组[注射0.1 mL FA+MTX混合液(90μg FA+5μg MTX溶于0.1 mL生理盐水中)],每组4个重复,每个重复15枚,利用PCR技术克隆鸡TNFAIP8L1基因开放阅读框(ORF),并对其进行生物信息学分析,同时利用荧光定量PCR方法分析FA和MTX对TNFAIP8L1基因在鸡胚孵化至第10天(10E)、第13天(13E)、第16天(16E)、第19天(19E)和出壳第1天(1D)肝脏中的表达差异。结果表明:克隆获得琅琊鸡TNFAIP8L1基因序列长726 bp,其中ORF区序列长561 bp,编码186个氨基酸。氨基酸多序列比对显示琅琊鸡与绿头鸭的氨基酸序列一致性最高(为97.85%),与马的一致性最低(为64.52%)。鸡TNFAIP8L1蛋白是一种亲水稳定蛋白;二级结构预测以α-螺旋(为72.04%)和无规则卷曲(为23.66%)为主要结构,三级结构与5jxd.1模板相似,其序列同源性为62.37%。10E和16E时,FA组和MTX组TNFAIP8L1基因相对表达量均显著低于生理盐水组(P<0.05);19E和1D时,FA组和MTX组TNFAIP8L1基因相对表达量与生理盐水组差异不显著(P>0.05)。说明在鸡胚发育前期FA和MTX可能对TNFAIP8L1基因表达有抑制作用。
基金supported by grants from the National Natural Science Foundation of China(82192862,82371157,82201303,and 82271173)the Natural Science Foundation of Jiangsu Province(BE2023653,BK20230025,and BK20200133)+1 种基金the China Postdoctoral Science Foundation(2020M681555)a Distinguished Young Scholar supported by the Medical Science and Technology Development Foundation,Nanjing Department of Health(JQX20003).
文摘Noise-induced hearing loss is a worldwide public health issue that is characterized by temporary or permanent changes in hearing sensitivity.This condition is closely linked to inflammatory responses,and interventions targeting the inflammatory gene tumor necrosis factoralpha(TNFα)are known to mitigate cochlear noise damage.TNFα-induced proteins(TNFAIPs)are a family of translucent acidic proteins,and TNFAIP6 has a notable association with inflammatory responses.To date,there have been few reports on TNFAIP6 levels in the inner ear.To elucidate the precise mechanism,we generated transgenic mouse models with conditional knockout of Tnfaip6(Tnfaip6 cKO).Evaluation of hair cell morphology and function revealed no significant differences in hair cell numbers or ribbon synapses between Tnfaip6 cKO and wild-type mice.Moreover,there were no notable variations in hair cell numbers or hearing function in noisy environments.Our results indicate that Tnfaip6 does not have a substantial impact on the auditory system.
基金funded by the Natural Science Basic Research Pro-gram of Shaanxi(no.2023-JC-QN-0876)Key Research and Development Program of Shaanxi(no.2023-YBSF-447).
文摘Background:There is growing evidence that the gene named tumor necrosis factorα–induced protein 6(TNFAIP6)has an important role in various tumors.However,a systematic pan-cancer analysis of TNFAIP6 is lacking.Here we aimed to analyze the expression of TNFAIP6 across multiple cancers and verify its expression during the progression of colon cancer.Methods:We performed a comprehensive bioinformatics analysis to examine the expression of TNFAIP6 across 27 tumor types.GEPIA2 was used to evaluate the effect of TNFAIP6 on clinical cancer prognosis.cBioportal was used to assess TNFAIP6 mutations.The correlation between TNFAIP6 and cancer immune infiltrates was explored using TIMER2.0.The CancerSEA database was used to perform functional analysis of TNFAIP6.Metascape was used to identify TNFAIP6-related gene enrichment pathways.Immunohistochemistry was performed to detect TNFAIP6 protein expression in the colon cancer.In addition,the Comparative Toxicogenomics Database was searched for known and possible antitumor drugs that may be associated with TNFAIP6.Results:We found that,in most of the cancers included in this analysis,TNFAIP6 was highly expressed,and there is a distinct relationship between TNFAIP6 expression and cancer prognosis.TNFAIP6 expression is associated with cancer-associated fibroblasts,neutrophils,and endothelial cells.TNFAIP6 and similar genes may also be involved in the PID_VEGF_VEGFR_pathway.Immunohistochemistry revealed an increasing trend of TNFAIP6 protein expression in normal,adenoma,and colon cancer tissues.Several known and possible antitumor drugs that may be associated with TNFAIP6 were identified in the Comparative Toxicogenomics Database.These results suggest that a number of drugsmay target TNFAIP6 during cancer treatment,including cisplatin,irinotecan,resveratrol,U 0126,NSC689534,genistein,NSC668394,oxaliplatin,plerixafor,topotecan,vincristine,flutamide,doxorubicin,MRK 003,folic acid,demecolcine,tunicamycin,zoledronic acid,and schizandrin B.Conclusions:TNFAIP6 may function as an oncogene in certain cancers.Furthermore,this study provides evidence that TNFAIP6 is an important factor in colon cancer progression.
文摘目的研究肿瘤坏死因子α诱导蛋白3(tumor necrosis factorα-induced protein 3,TNFAIP3)在食管鳞癌中表达的临床病理意义及其对鳞癌预后的判断价值。方法免疫组织化学法检测100例随访食管鳞癌及80例癌旁组织中TNFAIP3蛋白的表达,并分析了其与食管鳞癌临床病理特征及预后的关系。结果TNFAIP3在食管鳞癌中高表达阳性率为47.0%(47/100),显著高于癌旁组织中的高表达率15.0%(12/80),且与肿瘤的分化程度有关;生存分析显示高表达TNFAIP3的患者预后不良,单因素生存分析显示TNFAIP3的表达、肿瘤的浸润深度、淋巴结转移、临床分期与食管鳞癌预后密切相关,多因素生存分析显示TNFAIP3的表达、肿瘤的浸润深度、淋巴结转移、临床分期为食管鳞癌的独立预后预测因子。结论表达增高的TNFAIP3可能参与了食管鳞癌的发生发展,TNFAIP3的高表达可能成为食管鳞癌的独立预后因子。
