试验旨在探讨成年宁都黄鸡睾丸的蛋白质表达谱,筛选大小睾丸个体差异表达蛋白和功能通路,为繁殖性能选育提供基础数据。以6只22周龄宁都黄鸡的睾丸组织为研究对象,分为大睾丸组(H-TES)和小睾丸组(L-TES),采用串联质量标签(tandem mass t...试验旨在探讨成年宁都黄鸡睾丸的蛋白质表达谱,筛选大小睾丸个体差异表达蛋白和功能通路,为繁殖性能选育提供基础数据。以6只22周龄宁都黄鸡的睾丸组织为研究对象,分为大睾丸组(H-TES)和小睾丸组(L-TES),采用串联质量标签(tandem mass tags, TMT)技术对2组个体睾丸蛋白质进行差异表达、功能富集和基因互作分析,初步筛选候选蛋白质,分析这些蛋白质在6个个体的表达量与6个睾丸性状指标(左侧睾丸重、右侧睾丸重、总睾丸重、左侧睾丸指数、右侧睾丸指数、总睾丸指数)之间的相关性,分析候选蛋白质的功能。结果表明,宁都黄鸡睾丸中共鉴定到4 683个蛋白质,筛选到144个差异表达蛋白质。GO分析结果表明,差异蛋白质主要参与精子结构组成,具有尿蛋白-谷氨酸连接酶活性、胸腺嘧啶结合、脱氢抗坏血酸跨膜转运体活性等功能。差异蛋白质显著富集的KEGG通路为精氨酸和脯氨酸代谢、泛酸和辅酶A的生物合成、β-丙氨酸代谢等。蛋白质互作网络中有34个差异蛋白质,初步筛选出30个候选蛋白质。结合相关性分析和文献查阅,筛选出16个关键候选蛋白质,即SPAG6、TPPP2、ENKUR、ODF2、SAXO1、TCP11、DNALI1、CCDC63、TSSK3、TEKT2、TEKT3、ALDH2、DNAH5、CCDC40、CCDC39、DNAH10。结合关键候选蛋白质的功能,初步确定精氨酸和脯氨酸代谢、泛酸和辅酶A生物合成、β-丙氨酸代谢、紧密连接、间隙连接是睾丸性状调控关键KEGG通路。研究结果为地方鸡种睾丸质量性状选育提供了基础依据。展开更多
Objective The aim of the study was to screen key proteins involved in the treatment of human gastric cancer subcutaneous xenografts in nude mice by the traditional Chinese medicine formula Weichang’an and to explore ...Objective The aim of the study was to screen key proteins involved in the treatment of human gastric cancer subcutaneous xenografts in nude mice by the traditional Chinese medicine formula Weichang’an and to explore its mechanism of action in treating gastric cancer.Methods Sixteen 7-to 8-week-old female BALB/C nude mice were used to establish a human gastric cancer subcutaneous xenograft model by bilateral axillary injection of MKN45 cells.Mice with successfully established tumors were randomly divided into the Weichang’an group and the model group,with eight mice in each group.Mice in the Weichang’an group were orally administered 0.5mL of Weichang’an decoction,while mice in the model group were given 0.5 mL of normal saline by gavage once a day for 21 consecutive days.On day 28,the animals were sacrificed by cervical dislocation,and tumors were excised to measure tumor weight and assess the tumor suppression rate.Tandem mass tags(TMT)quantitative proteomics was used to analyze the tumor samples,identify differentially expressed proteins,and perform Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results Compared with the model group,the tumor weight in the Weichang’an group was significantly reduced(p<0.001),and the tumor suppression rate was 41.40%,indicating that Weichang’an can inhibit the growth of human gastric cancer subcutaneous xenografts in nude mice.TMT quantitative proteomics identified a total of 2,856 proteins,with 13 proteins showing downregulated expression and 25 proteins showing upregulated expression in the Weichang’an group.GO enrichment analysis revealed that differentially expressed proteins were mainly enriched in cellular components such as the cell membrane,extracellular matrix,and envelope,and participated in biological processes like negative regulation of cell adhesion hematopoiesis,and skeletal system development,with functions in cell adhesion molecule binding and molecular sensor activity.The KEGG pathway enrichment analysis identified the autophagy-lysosome pathway.Conclusion Weichang’an may exert its therapeutic effect on gastric cancer by regulating the expression of various proteins and modulating the autophagy-lysosome pathway.展开更多
文摘试验旨在探讨成年宁都黄鸡睾丸的蛋白质表达谱,筛选大小睾丸个体差异表达蛋白和功能通路,为繁殖性能选育提供基础数据。以6只22周龄宁都黄鸡的睾丸组织为研究对象,分为大睾丸组(H-TES)和小睾丸组(L-TES),采用串联质量标签(tandem mass tags, TMT)技术对2组个体睾丸蛋白质进行差异表达、功能富集和基因互作分析,初步筛选候选蛋白质,分析这些蛋白质在6个个体的表达量与6个睾丸性状指标(左侧睾丸重、右侧睾丸重、总睾丸重、左侧睾丸指数、右侧睾丸指数、总睾丸指数)之间的相关性,分析候选蛋白质的功能。结果表明,宁都黄鸡睾丸中共鉴定到4 683个蛋白质,筛选到144个差异表达蛋白质。GO分析结果表明,差异蛋白质主要参与精子结构组成,具有尿蛋白-谷氨酸连接酶活性、胸腺嘧啶结合、脱氢抗坏血酸跨膜转运体活性等功能。差异蛋白质显著富集的KEGG通路为精氨酸和脯氨酸代谢、泛酸和辅酶A的生物合成、β-丙氨酸代谢等。蛋白质互作网络中有34个差异蛋白质,初步筛选出30个候选蛋白质。结合相关性分析和文献查阅,筛选出16个关键候选蛋白质,即SPAG6、TPPP2、ENKUR、ODF2、SAXO1、TCP11、DNALI1、CCDC63、TSSK3、TEKT2、TEKT3、ALDH2、DNAH5、CCDC40、CCDC39、DNAH10。结合关键候选蛋白质的功能,初步确定精氨酸和脯氨酸代谢、泛酸和辅酶A生物合成、β-丙氨酸代谢、紧密连接、间隙连接是睾丸性状调控关键KEGG通路。研究结果为地方鸡种睾丸质量性状选育提供了基础依据。
基金funded by the Special Research Project of Traditional Chinese Medicine in Henan Province(2022ZY2031).
文摘Objective The aim of the study was to screen key proteins involved in the treatment of human gastric cancer subcutaneous xenografts in nude mice by the traditional Chinese medicine formula Weichang’an and to explore its mechanism of action in treating gastric cancer.Methods Sixteen 7-to 8-week-old female BALB/C nude mice were used to establish a human gastric cancer subcutaneous xenograft model by bilateral axillary injection of MKN45 cells.Mice with successfully established tumors were randomly divided into the Weichang’an group and the model group,with eight mice in each group.Mice in the Weichang’an group were orally administered 0.5mL of Weichang’an decoction,while mice in the model group were given 0.5 mL of normal saline by gavage once a day for 21 consecutive days.On day 28,the animals were sacrificed by cervical dislocation,and tumors were excised to measure tumor weight and assess the tumor suppression rate.Tandem mass tags(TMT)quantitative proteomics was used to analyze the tumor samples,identify differentially expressed proteins,and perform Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results Compared with the model group,the tumor weight in the Weichang’an group was significantly reduced(p<0.001),and the tumor suppression rate was 41.40%,indicating that Weichang’an can inhibit the growth of human gastric cancer subcutaneous xenografts in nude mice.TMT quantitative proteomics identified a total of 2,856 proteins,with 13 proteins showing downregulated expression and 25 proteins showing upregulated expression in the Weichang’an group.GO enrichment analysis revealed that differentially expressed proteins were mainly enriched in cellular components such as the cell membrane,extracellular matrix,and envelope,and participated in biological processes like negative regulation of cell adhesion hematopoiesis,and skeletal system development,with functions in cell adhesion molecule binding and molecular sensor activity.The KEGG pathway enrichment analysis identified the autophagy-lysosome pathway.Conclusion Weichang’an may exert its therapeutic effect on gastric cancer by regulating the expression of various proteins and modulating the autophagy-lysosome pathway.
