When the 2025 Intertextile Apparel Fabrics Exhibition(Autumn/Winter)was held in Shanghai,more than 3,700 top exhibitors from 26 countries and regions around the world participated.From September 2nd to 4th,the 2025&qu...When the 2025 Intertextile Apparel Fabrics Exhibition(Autumn/Winter)was held in Shanghai,more than 3,700 top exhibitors from 26 countries and regions around the world participated.From September 2nd to 4th,the 2025"Keqiao Selected"exhibition shone brightly at the event,showcasing the high-end quality of its products and the innovative strength of its regional brands.展开更多
Background:Vestibular migraine(VM)is a common disorder characterized by recurrent dizziness or vertigo,often aggravated by cold exposure.This study aimed to identify differentially expressed circular RNAs(circRNAs)in ...Background:Vestibular migraine(VM)is a common disorder characterized by recurrent dizziness or vertigo,often aggravated by cold exposure.This study aimed to identify differentially expressed circular RNAs(circRNAs)in cold-region VM and explore the underlying molecular mechanisms.Methods:Peripheral blood samples from long-term residents of Heilongjiang Province profiled by circRNA microarray,and differentially expressed circRNAs were validated by quantitative reverse transcription polymerase chain reaction(qRT-PCR).A competing endogenous RNA(ceRNA)network and enriched pathways were inferred by bioinformatics.A VM-like mouse model was established using nitroglycerin(NTG)and kainic acid(KA)and confirmed by behavioral testing and western blot.The hsa_circ_0003201/miR-31-5p/triggering receptor expressed on myeloid cells 2(TREM2)axis and related pathways were examined in clinical samples and in the trigeminal nucleus caudalis(TNC)and vestibular nuclei(VN)of mice using qRT-PCR,enzyme-linked immunosorbent assay(ELISA),and western blot.CircRNA microarray profiling also compared expression patterns between VM and migraine patients.Results:Hsa_circ_0003201 was significantly upregulated in cold-region VM patients.Bioinformatic analyses revealed that hsa_circ_0003201 may regulate the miR-31-5p/TREM2 axis and be associated with phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)signaling,pyruvate metabolism,and transient receptor potential(TRP)pathways.Clinical validation confirmed increased hsa_circ_0003201 and TREM2 and decreased miR-31-5p.VM-like mice exhibited central sensitization and vestibular dysfunction,with increased TREM2,decreased miR-31-5p,and PI3K/AKT activation in the TNC and VN.Comparative circRNA analysis between VM and migraine patients indicated distinct expression patterns.Conclusion:Hsa_circ_0003201 shows potential as a diagnostic biomarker for cold-region VM,and the hsa_circ_0003201/miR-31-5p/TREM2 axis may contribute to pathogenesis through PI3K/AKT signaling,pyruvate metabolism,and TRP-related pathways.展开更多
Before nationwide implementation of waste classification programs in rural areas,identifying suitable pilot regions is crucial.This study develops a comprehensive framework for assessing and selecting these pilot regi...Before nationwide implementation of waste classification programs in rural areas,identifying suitable pilot regions is crucial.This study develops a comprehensive framework for assessing and selecting these pilot regions.Data were collected through 654 valid questionnaires and interviews with respondents from rural areas in six provinces across China.Primary assessment criteria included disposal methods for kitchen waste,recyclables,hazardous waste,and residual waste,as well as farmers’willingness to separate hazardous waste when offered compensation and the impact of centralized waste treatment in villages.Secondary criteria focused on disposal methods for agrochemical packages and other hazardous waste.Using the weighted sum of squares method,the study evaluated the suitability of six regions as pilot areas for waste classification.The results identified villages in Jiangsu province and Hebei province as the most suitable pilot regions.Based on the findings,the study provides recommendations to address weaknesses in these regions to enhance the effectiveness of future trials and offers tailored suggestions for other regions to improve their waste classification initiatives.展开更多
Background Sperm cryopreservation is widely used in the cattle industry,as it allows for disassociating the localiza-tion of sires and the collection of semen from the timing of artificial insemination.While freeze-th...Background Sperm cryopreservation is widely used in the cattle industry,as it allows for disassociating the localiza-tion of sires and the collection of semen from the timing of artificial insemination.While freeze-thawing is known to impair sperm DNA integrity,whether the damage induced consists of single-(SSB)or double-strand breaks(DSB)has not been determined.In addition,no previous study has addressed if DNA breaks preferentially reside in specific genome regions such as those forming the toroid linker regions,or are rather spread throughout the regions linked to protamines.The main aim of the present work,therefore,was to elucidate the type and localization of the DNA damage generated by cryopreservation and to evaluate its impact on artificial insemination outcomes in cattle.Results The incidence of SSB and DSB was evaluated in 12 ejaculates before and after cryopreservation with the Comet assay,and the localization of the DNA breaks was assessed using pulsed-field gel electrophoresis(PFGE).Before cryopreservation,the incidence of SSB was 10.99%±4.62%and involved 20.56%±3.04%of sperm cells,whereas these figures significantly(P<0.0001)increased up to 34.11%±3.48%and 53.36%±11.00%in frozen-thawed sperm.In contrast,no significant differences in the incidence of DSB were observed(P>0.990)before and after cryopreservation(before:incidence of 13.91%±1.75%of sperm DNA affecting 56.04%±12.49%of sperm cells;after:incidence of 13.55%±1.55%of sperm DNA involving 53.36%±11.00%of sperm cells).Moreover,PFGE revealed that the percentage of sperm DNA fragments whose length was shorter than a toroid(<31.5 kb)was greater(P<0.0001)after(27.00%±4.26%)than before freeze-thawing(15.57%±4.53%).These differences indicated that the DNA breaks induced by cryopreservation affect the regions condensed in protamines,which are structured in toroids.On the other hand,in vivo fertility rates were associated to the incidence of SSB and DSB in frozen-thawed sperm(P=0.032 and P=0.005),but not with the size of the DNA fragments resulting from these breaks(P>0.05).Conclusion Cryopreservation of bovine sperm generates single-strand DNA breaks,which are mainly located in protamine-condensed toroidal regions.The incidence of DNA breaks in cryopreserved sperm has an impact on cat-tle fertility,regardless of the size of generated fragments.展开更多
In many existing dictionaries,cropland is defined as land that is suitable for or used to grow crops.It has several synonyms,such as“farmland”,“arable land”,and“cultivated land”.However,in scientific literature,...In many existing dictionaries,cropland is defined as land that is suitable for or used to grow crops.It has several synonyms,such as“farmland”,“arable land”,and“cultivated land”.However,in scientific literature,the nuances of these terms are often overlooked.The inconsistent terminology usage could lead to ambiguity and confusion in research and policy discussions.In particular,it creates difficulties for newcomers and students when they search for precise information in the published literature.Hence,exploring the variations of terminology applications is important for the cropland-related research community.In this study,the differences in six cropland-related terminologies were explored through a review of 5,214 scientific articles,by employing the independence test,clustering approach,and correlation analysis.The results showed that disparities exist across disciplines.For example,biodiversity&conservation studies preferentially use“farmland”to highlight effects from human activities,while studies in geology and computer science use“cropland”.The term“cultivated land”tends to be used in geography research for clear geographical demarcation,while“arable land”is related to engineering studies.Moreover,further disparities based on the geographical affiliations of the authors were found.The correlation between China and“cultivated land”was reliable and a close link was found between“agricultural land”and the USA.The regional variations in cropland terminology can be influenced by multiple factors,including the degree of agricultural mechanization,colonial history,and migration patterns.This study reveals variations in cropland-related terminology across disciplines and regions.The results highlight the importance of standardizing cropland terminology to foster interdisciplinary research,improve data comparability,and support global agricultural and environmental policymaking.展开更多
Surface albedo,as one of the important properties of the underlying surface,has a significant impact on the surface energy balance in cold regions.However,due to the complexity of the factors affecting surface albedo,...Surface albedo,as one of the important properties of the underlying surface,has a significant impact on the surface energy balance in cold regions.However,due to the complexity of the factors affecting surface albedo,existing calculations still contain inaccuracies.Therefore,this study conducted surface albedo experiments on loess with different water contents and temperatures.By analyzing the surface albedo measurements of samples with varying temperature and water content levels,as well as the soil freezing characteristic curve(SFCC)and soil-water characteristic curve(SWCC)of loess,the study explores the influence of soil temperature and water content on the surface albedo of loess.The results indicate that both the temperature and water content of loess jointly affect surface albedo.During the process of albedo change,there exists a water content threshold that alters the trend of surface albedo.Soil temperature influences surface albedo by affecting the content of pore ice and liquid water within the soil.When the water content of loess is relatively low,the surface albedo decreases as the unfrozen water content decreases.However,this trend changes as the water content of loess increases.Additionally,a decrease in soil temperature lowers the moisture content threshold during the changes in surface albedo.This study provides a reference for exploring and determining the surface energy balance in cold regions under the background of warm and humid climates,as well as for establishing thermal calculation boundaries.展开更多
BACKGROUND Metabolomics sequencing technology was used to investigate the changes of intestinal flora and metabolites in gastric cancer patients in plateau areas.AIM To investigate changes in gut microbiota and their ...BACKGROUND Metabolomics sequencing technology was used to investigate the changes of intestinal flora and metabolites in gastric cancer patients in plateau areas.AIM To investigate changes in gut microbiota and their metabolites in patients with gastric cancer from plateau regions using untargeted metabolomic sequencing.METHODS Fresh morning fecal samples were collected from 30 gastric cancer patients diagnosed at a tertiary hospital in Qinghai Province and 30 healthy individuals(controls).Liquid chromatography-tandem mass spectrometry based untargeted metabolomic sequencing was used to analyze metabolite changes and predict metabolic function.RESULTS Metabolomic analysis identified 281 metabolites in samples from both groups.These metabolites were categorized into eight major classes,listed in descending order of abundance:Lipids and lipid-like molecules(35.443%);organic acids and derivatives(29.114%);organic oxygen compounds(15.19%);nucleosides,nucleotides,and analogs(13.924%);organoheterocyclic compounds(2.532%),amino acids and peptides(1.266%);benzenoids(1.266%);and fatty acids(1.266%).Compared with the control group,the top 10 metabolites elevated in the gastric cancer group included:Dethiobiotin,glycylproline,glycine,hydroxyisocaproic acid,tyramine,methionine sulfoxide,5-aminopentanoic acid,citrulline,betonicine,and formiminoglutamic acid and the top 10 decreased were:Cytidine,5'-methylthioadenosine,trehalose,melibiose,lotaustralin,adenosine,inosine,ribothymidine,raffinose,and galactinol.Functional prediction analysis revealed that these differential metabolites were primarily enriched in 12 metabolic pathways,including purine metabolism,cysteine and methionine metabolism,galactose metabolism,lysine degradation,glycine,serine,and threonine metabolism,biotin metabolism,pyrimidine metabolism,arginine and proline metabolism,histidine metabolism,primary bile acid biosynthesis,starch and sucrose metabolism,and tyrosine metabolism.CONCLUSION Significant differences in intestinal microbial metabolites and associated metabolic pathways were observed between gastric cancer patients and healthy controls residing in plateau regions.展开更多
Branch length and branch diameter are important characteristics that determine wood quality and yield.Development of static branch length and diameter models by incorporating individual tree variables,site quality and...Branch length and branch diameter are important characteristics that determine wood quality and yield.Development of static branch length and diameter models by incorporating individual tree variables,site quality and competition have been widely studied,while the climate effect has rarely been reported.In this study,mixed-effects climate-sensitive branch length and diameter models were developed based on 228 sample trees of Larix kaempferi from three latitude regions in China(approximate 42°N in Liaoning Province,33°N in Gansu Province,and 30°N in Hubei Province).Results revealed that diameter at breast height,and crown ratio,sum of the basal areas of trees larger than the subject trees,dominant tree height,mean warmest month temperature,and summer precipitation substantially improved branch length model.Diameter at breast height,and crown ratio,ratio of the sum of DBH in sample plot to the subject tree,dominant tree height,mean warmest month temperature,and spring precipitation significantly improved branch diameter model.Compared with base model,mean square error reduction of mixed-effects branch length and diameter models were 32.9%and 44.1%,respectively.The relative contributions of covariates to branch length model were tree size(59.1%),site quality(25.7%),competition(13.5%),and climate(1.7%),and branch diameter model were tree size(57.0%),competition(21.9%),site quality(18.3%),and climate(2.8%).Relative contributions of covariates on branch length and diameter models from different latitude regions were different.Effects of competition on branch length model in Liaoning and Hubei were larger than climate,whereas climate in Gansu was larger than competition.As for branch diameter model,competition in Liaoning was larger than site quality,whereas site quality in Hubei and Gansu was larger than competition.The present study strengthened the importance of considering climate variables in developing branch length and diameter model.It is desirable to disentangle the different sources of variations in affecting branch length and diameter from different latitude regions to reduce the uncertainty in predicting branch characteristics under the condition of climate changing.展开更多
Economic development,food security,and ecological preservation are important issues encountered by karst re-gions.Faced with complex natural and social dynamics,we attempted to explore how interdependence within socio...Economic development,food security,and ecological preservation are important issues encountered by karst re-gions.Faced with complex natural and social dynamics,we attempted to explore how interdependence within socio-ecological system(SES)shaped sustainability in this region.A SES framework was constructed and three scenarios were predesigned:economic priority scenario,food security scenario,and ecological protection sce-nario.The System Dynamics model was used to simulate and forecast the evolution across various scenarios within the SES from 2005 to 2035.Through the Production-Possibility Frontiers in combined scenarios,trade-offpotential was identified and quantified.The results showed that the decoupling between social and ecological subsystems can be weaken in economic priority scenario,while coupling between them can be strengthen in food security scenario and ecological protection scenario.Within the SES,combined scenario analyses further suggest that the rocky desertification rate and the urban-rural income ratio exhibit the least trade-offpotential and inten-sity in combined economic priority scenario and ecological protection scenario,and the Soil Conservation and Food Supply demonstrate the least trade-offpotential and intensity in combined economic priority scenario and food security scenario.We can conclude the ecological engineering plays a significant role in alleviating trade-offs within the SES,but the effectiveness is limited.In light of intertwined socio-ecological challenges,combining ecological engineering with adaptive adjustments is a crucial strategy to enhance SES resilience and promote sustainable development in the South China Karst.展开更多
The ITMA ASIA+CITME,Singapore 2025 will be held from October 28-31,2025 at the Singapore EXPO.Co-organized by the European Committee of Textile Machinery Manufacturers(CEMATEX),the China Textile Machinery Association(...The ITMA ASIA+CITME,Singapore 2025 will be held from October 28-31,2025 at the Singapore EXPO.Co-organized by the European Committee of Textile Machinery Manufacturers(CEMATEX),the China Textile Machinery Association(CTMA),and the Sub-Council of Textile Industry,China Council for the Promotion of International Trade(CCPIT TEX),Centered on the theme"Leading Textile Technology Exhibition Driving Regional Growth",this event convenes global leading textile machinery manufacturers,technical experts,and industry leaders to jointly explore innovation and the future of the textile sector.展开更多
Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in th...Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in this area.Therefore,the purpose of this study was:(1)to assess the status of self-management capacity in individuals with CKD in cold regions of China and analyze the factors influencing it;(2)to identify strategies to improve CKD management in primary care settings in these regions;and(3)to understand patients'attitudes toward eHealth services.Methods:This was a regional,cross-sectional observational study.A questionnaire measuring CKD patients'self-management abilities was derived from the Perceived Kidney Disease Self-Management Scale,the Kidney Disease Behavioral Inventory(KDBI),and the Health Literacy Questionnaire.Data were collected from hospitalized CKD patients in Heilongjiang Province and analyzed using One-Way Analysis of Variance(ANOVA),Hierarchical Regression Analysis,and K-prototype cluster analysis.Results:A total of 957 participants were tested.Of these,70.64%had less than a bachelor's degree,and 56.27%had been diagnosed with hypertensive or diabetic nephropathy.The KDBI scale showed a lower overall score compared to the PKDSMS.Factors such as CKD stage 4(F=2.367,P=0.042),last year's medical expenses(F=3.974,P=0.004),and poor self-rated health(F=33.352,P<0.01)were found to influence scores on both scales.The health literacy questionnaire revealed significant differences(P<0.01)in health knowledge,except by sex.Additionally,healthcare expenditures and poor self-rated health were negatively associated with self-management capacity.Conclusion:This study provides valuable insights into the self-management challenges faced by CKD patients in cold regions of China.Despite some difficulties in improving self-management,patients showed positive attitudes toward enhancing CKD management services in primary care and developing digital management tools.These findings offer useful references and recommendations for future clinical practice and research in this field.展开更多
Permanently shadowed regions(PSRs)on the Moon are potential reservoirs for water ice,making them hot spots for future lunar exploration.The water ice in PSRs would cause distinctive changes in space weathering there,i...Permanently shadowed regions(PSRs)on the Moon are potential reservoirs for water ice,making them hot spots for future lunar exploration.The water ice in PSRs would cause distinctive changes in space weathering there,in particular reduction-oxidation processes that diff er from those in illuminated regions.To determine the characteristics of products formed during space weathering in PSRs,the lunar meteorite NWA 10203 with artifi cially added water was irradiated with a nanosecond laser to simulate a micro-meteorite bombardment of lunar soil containing water ice.The TEM results of the water-incorporated sample showed distinct amorphous rims that exhibited irregular thickness,poor stratifi cation,the appearance of bubbles,and a reduced number of npFe^(0).Additionally,EELS analysis showed the presence of ferric iron at the rim of the nanophase metallic iron particles(npFe^(0))in the amorphous rim with the involvement of water.The results suggest that water ice is another possible factor contributing to oxidation during micrometeorite bombardment on the lunar surface.In addition,it off ers a reference for a new space weathering model that incorporates water in PSRs,which could be widespread on asteroids with volatiles.展开更多
Fermented chili products are popular traditional fermented foods around the world.However,differences in microbial communities in fermented chilies from different regions and how they affect the flavor compounds in ch...Fermented chili products are popular traditional fermented foods around the world.However,differences in microbial communities in fermented chilies from different regions and how they affect the flavor compounds in chili fermentation have not been reported.In this study,the dynamics of flavor compounds and microbial communities in fermented chilies from Sichuan,Guizhou,and Hunan were systematically investigated by macro-genome sequencing,solid phase microextraction-gas chromatograph-mass spectrometry(SPME-GC-MS),electronic nose,and electronic tongue techniques.Simultaneously,the microbial metabolic mechanisms and the relationship between flavor compounds and microbiome were unraveled through staged and simulated fermentation analysis.The results showed that 53 chemical odorants,including alcohols,esters,aldehydes,and acids,were identified as chemical markers to differentiate the regional samples.A total of 12 microbial species,including Staphylococcus xylosus,unclassified Staphylococcus species,Weissella confusus,Lactococcus cremoris,Lactococcus garvieae,Lactiplantibacillus sakei,Pediciococcus propionicigenes,Pediciococcus idahonensis,Pediciococcus aciditolerans,Nocardioides antri,Debaryomyces hansenii,and Colletotrichum scovillei,were identified as microbial markers to differentiate the regional samples.Correlation analysis showed that Lactobacillaceae was associated with fruity,floral,spicy,and fatty aromas.The electronic nose and tongue analysis results showed that 9 flavor and 8 taste indicators significantly differed between regional samples(P<0.05).Additionally,flavor compounds and microbial diversity were robust under initial selection stress and showed higher diversity under metabolome-microbiome interactions.Importantly,simulated fermentation confirmed that metabolome-microbiome interactions drove the shift in microbial structure,metabolism,and flavor in regionally fermented chilies.These results provide insights into the succession of microbial communities and the formation of flavor compounds in chili fermentation,which may enable the future replication of fermented foods with the same flavor.展开更多
文摘When the 2025 Intertextile Apparel Fabrics Exhibition(Autumn/Winter)was held in Shanghai,more than 3,700 top exhibitors from 26 countries and regions around the world participated.From September 2nd to 4th,the 2025"Keqiao Selected"exhibition shone brightly at the event,showcasing the high-end quality of its products and the innovative strength of its regional brands.
基金supported by General Program of National Natural Science Foundation of China(No.82071549,82371483)Key Research&Development Program of Heilongjiang(No.2023ZX06C02)Youth Foundation of the first Affiliated Hospital of Harbin Medical University(No.2024YQ22).
文摘Background:Vestibular migraine(VM)is a common disorder characterized by recurrent dizziness or vertigo,often aggravated by cold exposure.This study aimed to identify differentially expressed circular RNAs(circRNAs)in cold-region VM and explore the underlying molecular mechanisms.Methods:Peripheral blood samples from long-term residents of Heilongjiang Province profiled by circRNA microarray,and differentially expressed circRNAs were validated by quantitative reverse transcription polymerase chain reaction(qRT-PCR).A competing endogenous RNA(ceRNA)network and enriched pathways were inferred by bioinformatics.A VM-like mouse model was established using nitroglycerin(NTG)and kainic acid(KA)and confirmed by behavioral testing and western blot.The hsa_circ_0003201/miR-31-5p/triggering receptor expressed on myeloid cells 2(TREM2)axis and related pathways were examined in clinical samples and in the trigeminal nucleus caudalis(TNC)and vestibular nuclei(VN)of mice using qRT-PCR,enzyme-linked immunosorbent assay(ELISA),and western blot.CircRNA microarray profiling also compared expression patterns between VM and migraine patients.Results:Hsa_circ_0003201 was significantly upregulated in cold-region VM patients.Bioinformatic analyses revealed that hsa_circ_0003201 may regulate the miR-31-5p/TREM2 axis and be associated with phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)signaling,pyruvate metabolism,and transient receptor potential(TRP)pathways.Clinical validation confirmed increased hsa_circ_0003201 and TREM2 and decreased miR-31-5p.VM-like mice exhibited central sensitization and vestibular dysfunction,with increased TREM2,decreased miR-31-5p,and PI3K/AKT activation in the TNC and VN.Comparative circRNA analysis between VM and migraine patients indicated distinct expression patterns.Conclusion:Hsa_circ_0003201 shows potential as a diagnostic biomarker for cold-region VM,and the hsa_circ_0003201/miR-31-5p/TREM2 axis may contribute to pathogenesis through PI3K/AKT signaling,pyruvate metabolism,and TRP-related pathways.
基金supported by the Yunnan Provincial Education Department Scientific Research Fund Project(Grant No.2022J0525).
文摘Before nationwide implementation of waste classification programs in rural areas,identifying suitable pilot regions is crucial.This study develops a comprehensive framework for assessing and selecting these pilot regions.Data were collected through 654 valid questionnaires and interviews with respondents from rural areas in six provinces across China.Primary assessment criteria included disposal methods for kitchen waste,recyclables,hazardous waste,and residual waste,as well as farmers’willingness to separate hazardous waste when offered compensation and the impact of centralized waste treatment in villages.Secondary criteria focused on disposal methods for agrochemical packages and other hazardous waste.Using the weighted sum of squares method,the study evaluated the suitability of six regions as pilot areas for waste classification.The results identified villages in Jiangsu province and Hebei province as the most suitable pilot regions.Based on the findings,the study provides recommendations to address weaknesses in these regions to enhance the effectiveness of future trials and offers tailored suggestions for other regions to improve their waste classification initiatives.
基金Ministry of Science,Innovation and Universities,Spain(NextGeneration EU fundsMaría Zambrano Program 124/MTAI/22+2 种基金and PID2020-113320RB-I00)Agency for Management of University and Research Grants,Regional Government of Catalonia,Spain(2021-SGR-00900)Catalan Institution for Research and Advanced Studies(ICREA).
文摘Background Sperm cryopreservation is widely used in the cattle industry,as it allows for disassociating the localiza-tion of sires and the collection of semen from the timing of artificial insemination.While freeze-thawing is known to impair sperm DNA integrity,whether the damage induced consists of single-(SSB)or double-strand breaks(DSB)has not been determined.In addition,no previous study has addressed if DNA breaks preferentially reside in specific genome regions such as those forming the toroid linker regions,or are rather spread throughout the regions linked to protamines.The main aim of the present work,therefore,was to elucidate the type and localization of the DNA damage generated by cryopreservation and to evaluate its impact on artificial insemination outcomes in cattle.Results The incidence of SSB and DSB was evaluated in 12 ejaculates before and after cryopreservation with the Comet assay,and the localization of the DNA breaks was assessed using pulsed-field gel electrophoresis(PFGE).Before cryopreservation,the incidence of SSB was 10.99%±4.62%and involved 20.56%±3.04%of sperm cells,whereas these figures significantly(P<0.0001)increased up to 34.11%±3.48%and 53.36%±11.00%in frozen-thawed sperm.In contrast,no significant differences in the incidence of DSB were observed(P>0.990)before and after cryopreservation(before:incidence of 13.91%±1.75%of sperm DNA affecting 56.04%±12.49%of sperm cells;after:incidence of 13.55%±1.55%of sperm DNA involving 53.36%±11.00%of sperm cells).Moreover,PFGE revealed that the percentage of sperm DNA fragments whose length was shorter than a toroid(<31.5 kb)was greater(P<0.0001)after(27.00%±4.26%)than before freeze-thawing(15.57%±4.53%).These differences indicated that the DNA breaks induced by cryopreservation affect the regions condensed in protamines,which are structured in toroids.On the other hand,in vivo fertility rates were associated to the incidence of SSB and DSB in frozen-thawed sperm(P=0.032 and P=0.005),but not with the size of the DNA fragments resulting from these breaks(P>0.05).Conclusion Cryopreservation of bovine sperm generates single-strand DNA breaks,which are mainly located in protamine-condensed toroidal regions.The incidence of DNA breaks in cryopreserved sperm has an impact on cat-tle fertility,regardless of the size of generated fragments.
基金supported by the National Key Research and Development Program of China(2023YFD2300501)the National Natural Science Foundation of China(41921001 and 42401440)。
文摘In many existing dictionaries,cropland is defined as land that is suitable for or used to grow crops.It has several synonyms,such as“farmland”,“arable land”,and“cultivated land”.However,in scientific literature,the nuances of these terms are often overlooked.The inconsistent terminology usage could lead to ambiguity and confusion in research and policy discussions.In particular,it creates difficulties for newcomers and students when they search for precise information in the published literature.Hence,exploring the variations of terminology applications is important for the cropland-related research community.In this study,the differences in six cropland-related terminologies were explored through a review of 5,214 scientific articles,by employing the independence test,clustering approach,and correlation analysis.The results showed that disparities exist across disciplines.For example,biodiversity&conservation studies preferentially use“farmland”to highlight effects from human activities,while studies in geology and computer science use“cropland”.The term“cultivated land”tends to be used in geography research for clear geographical demarcation,while“arable land”is related to engineering studies.Moreover,further disparities based on the geographical affiliations of the authors were found.The correlation between China and“cultivated land”was reliable and a close link was found between“agricultural land”and the USA.The regional variations in cropland terminology can be influenced by multiple factors,including the degree of agricultural mechanization,colonial history,and migration patterns.This study reveals variations in cropland-related terminology across disciplines and regions.The results highlight the importance of standardizing cropland terminology to foster interdisciplinary research,improve data comparability,and support global agricultural and environmental policymaking.
基金supported by the National Natural Science Foundation of China(42261028)the Chinese Academy of Sciences“Light of West China”Program for Western Young Scholars(23JR6KA027)+3 种基金the Science Foundation for Distinguished Young Scholars of Gansu Province(24JRRA167)the Key Research and Development Program on Ecological Civilization Construction of Gansu Province(25YFFA012)Gansu Provincial Science and Technology Plan Fund Project(24CXGA063)Scientific Research Projects on Ecological and Environmental Protection in Heilongjiang Province in 2023(Grant No.:HST2023ZR005)。
文摘Surface albedo,as one of the important properties of the underlying surface,has a significant impact on the surface energy balance in cold regions.However,due to the complexity of the factors affecting surface albedo,existing calculations still contain inaccuracies.Therefore,this study conducted surface albedo experiments on loess with different water contents and temperatures.By analyzing the surface albedo measurements of samples with varying temperature and water content levels,as well as the soil freezing characteristic curve(SFCC)and soil-water characteristic curve(SWCC)of loess,the study explores the influence of soil temperature and water content on the surface albedo of loess.The results indicate that both the temperature and water content of loess jointly affect surface albedo.During the process of albedo change,there exists a water content threshold that alters the trend of surface albedo.Soil temperature influences surface albedo by affecting the content of pore ice and liquid water within the soil.When the water content of loess is relatively low,the surface albedo decreases as the unfrozen water content decreases.However,this trend changes as the water content of loess increases.Additionally,a decrease in soil temperature lowers the moisture content threshold during the changes in surface albedo.This study provides a reference for exploring and determining the surface energy balance in cold regions under the background of warm and humid climates,as well as for establishing thermal calculation boundaries.
基金Supported by Gansu Provincial Natural Science Foundation Project,No.23JRRA725Postgraduate Research Innovation Project of Northwest Minzu University in 2025,No.31920250001-382024 Qinghai Province's"Kunlun Talent High-end Innovative and Entrepreneurial Talent Project"Cultivates Top Talents Project,No.QHKLYC-GDCXCY-2024-155.
文摘BACKGROUND Metabolomics sequencing technology was used to investigate the changes of intestinal flora and metabolites in gastric cancer patients in plateau areas.AIM To investigate changes in gut microbiota and their metabolites in patients with gastric cancer from plateau regions using untargeted metabolomic sequencing.METHODS Fresh morning fecal samples were collected from 30 gastric cancer patients diagnosed at a tertiary hospital in Qinghai Province and 30 healthy individuals(controls).Liquid chromatography-tandem mass spectrometry based untargeted metabolomic sequencing was used to analyze metabolite changes and predict metabolic function.RESULTS Metabolomic analysis identified 281 metabolites in samples from both groups.These metabolites were categorized into eight major classes,listed in descending order of abundance:Lipids and lipid-like molecules(35.443%);organic acids and derivatives(29.114%);organic oxygen compounds(15.19%);nucleosides,nucleotides,and analogs(13.924%);organoheterocyclic compounds(2.532%),amino acids and peptides(1.266%);benzenoids(1.266%);and fatty acids(1.266%).Compared with the control group,the top 10 metabolites elevated in the gastric cancer group included:Dethiobiotin,glycylproline,glycine,hydroxyisocaproic acid,tyramine,methionine sulfoxide,5-aminopentanoic acid,citrulline,betonicine,and formiminoglutamic acid and the top 10 decreased were:Cytidine,5'-methylthioadenosine,trehalose,melibiose,lotaustralin,adenosine,inosine,ribothymidine,raffinose,and galactinol.Functional prediction analysis revealed that these differential metabolites were primarily enriched in 12 metabolic pathways,including purine metabolism,cysteine and methionine metabolism,galactose metabolism,lysine degradation,glycine,serine,and threonine metabolism,biotin metabolism,pyrimidine metabolism,arginine and proline metabolism,histidine metabolism,primary bile acid biosynthesis,starch and sucrose metabolism,and tyrosine metabolism.CONCLUSION Significant differences in intestinal microbial metabolites and associated metabolic pathways were observed between gastric cancer patients and healthy controls residing in plateau regions.
基金funded by the National Key Research and Development Program of China(2023YFD2200801)National Natural Science Foundation of China(No.32471864)+1 种基金the Science&Technology Project for Excellent Young Scholars of Liaoning Province(2024JH3/10200036)Liao Ning Revitalization Talents Program(No.XLYC2403180).
文摘Branch length and branch diameter are important characteristics that determine wood quality and yield.Development of static branch length and diameter models by incorporating individual tree variables,site quality and competition have been widely studied,while the climate effect has rarely been reported.In this study,mixed-effects climate-sensitive branch length and diameter models were developed based on 228 sample trees of Larix kaempferi from three latitude regions in China(approximate 42°N in Liaoning Province,33°N in Gansu Province,and 30°N in Hubei Province).Results revealed that diameter at breast height,and crown ratio,sum of the basal areas of trees larger than the subject trees,dominant tree height,mean warmest month temperature,and summer precipitation substantially improved branch length model.Diameter at breast height,and crown ratio,ratio of the sum of DBH in sample plot to the subject tree,dominant tree height,mean warmest month temperature,and spring precipitation significantly improved branch diameter model.Compared with base model,mean square error reduction of mixed-effects branch length and diameter models were 32.9%and 44.1%,respectively.The relative contributions of covariates to branch length model were tree size(59.1%),site quality(25.7%),competition(13.5%),and climate(1.7%),and branch diameter model were tree size(57.0%),competition(21.9%),site quality(18.3%),and climate(2.8%).Relative contributions of covariates on branch length and diameter models from different latitude regions were different.Effects of competition on branch length model in Liaoning and Hubei were larger than climate,whereas climate in Gansu was larger than competition.As for branch diameter model,competition in Liaoning was larger than site quality,whereas site quality in Hubei and Gansu was larger than competition.The present study strengthened the importance of considering climate variables in developing branch length and diameter model.It is desirable to disentangle the different sources of variations in affecting branch length and diameter from different latitude regions to reduce the uncertainty in predicting branch characteristics under the condition of climate changing.
基金supported by the National Key Research and Develop-ment Program of China(Grant No.2022YFF1300701)the Sichuan Science and Technology Program(Grant No.2022JDJQ0015).
文摘Economic development,food security,and ecological preservation are important issues encountered by karst re-gions.Faced with complex natural and social dynamics,we attempted to explore how interdependence within socio-ecological system(SES)shaped sustainability in this region.A SES framework was constructed and three scenarios were predesigned:economic priority scenario,food security scenario,and ecological protection sce-nario.The System Dynamics model was used to simulate and forecast the evolution across various scenarios within the SES from 2005 to 2035.Through the Production-Possibility Frontiers in combined scenarios,trade-offpotential was identified and quantified.The results showed that the decoupling between social and ecological subsystems can be weaken in economic priority scenario,while coupling between them can be strengthen in food security scenario and ecological protection scenario.Within the SES,combined scenario analyses further suggest that the rocky desertification rate and the urban-rural income ratio exhibit the least trade-offpotential and inten-sity in combined economic priority scenario and ecological protection scenario,and the Soil Conservation and Food Supply demonstrate the least trade-offpotential and intensity in combined economic priority scenario and food security scenario.We can conclude the ecological engineering plays a significant role in alleviating trade-offs within the SES,but the effectiveness is limited.In light of intertwined socio-ecological challenges,combining ecological engineering with adaptive adjustments is a crucial strategy to enhance SES resilience and promote sustainable development in the South China Karst.
文摘The ITMA ASIA+CITME,Singapore 2025 will be held from October 28-31,2025 at the Singapore EXPO.Co-organized by the European Committee of Textile Machinery Manufacturers(CEMATEX),the China Textile Machinery Association(CTMA),and the Sub-Council of Textile Industry,China Council for the Promotion of International Trade(CCPIT TEX),Centered on the theme"Leading Textile Technology Exhibition Driving Regional Growth",this event convenes global leading textile machinery manufacturers,technical experts,and industry leaders to jointly explore innovation and the future of the textile sector.
基金supported by the Natural Science Foundation of China(7247042122)the Key Research and Development Project of Heilongjiang Province,China(2024ZX12C06).
文摘Objective:Effective Chronic Kidney Disease(CKD)management is particularly important in cold regions of China,where climate and lifestyle factors play significant roles.However,there is a lack of relevant studies in this area.Therefore,the purpose of this study was:(1)to assess the status of self-management capacity in individuals with CKD in cold regions of China and analyze the factors influencing it;(2)to identify strategies to improve CKD management in primary care settings in these regions;and(3)to understand patients'attitudes toward eHealth services.Methods:This was a regional,cross-sectional observational study.A questionnaire measuring CKD patients'self-management abilities was derived from the Perceived Kidney Disease Self-Management Scale,the Kidney Disease Behavioral Inventory(KDBI),and the Health Literacy Questionnaire.Data were collected from hospitalized CKD patients in Heilongjiang Province and analyzed using One-Way Analysis of Variance(ANOVA),Hierarchical Regression Analysis,and K-prototype cluster analysis.Results:A total of 957 participants were tested.Of these,70.64%had less than a bachelor's degree,and 56.27%had been diagnosed with hypertensive or diabetic nephropathy.The KDBI scale showed a lower overall score compared to the PKDSMS.Factors such as CKD stage 4(F=2.367,P=0.042),last year's medical expenses(F=3.974,P=0.004),and poor self-rated health(F=33.352,P<0.01)were found to influence scores on both scales.The health literacy questionnaire revealed significant differences(P<0.01)in health knowledge,except by sex.Additionally,healthcare expenditures and poor self-rated health were negatively associated with self-management capacity.Conclusion:This study provides valuable insights into the self-management challenges faced by CKD patients in cold regions of China.Despite some difficulties in improving self-management,patients showed positive attitudes toward enhancing CKD management services in primary care and developing digital management tools.These findings offer useful references and recommendations for future clinical practice and research in this field.
基金support from the Youth Innovation Promotion Association,Chinese Academy of Sciences(2020395)Strategic Priority Research Program of the Chinese Academy of Sciences grant XDB 41000000(Y.L.)+4 种基金National Natural Science Foundation of China(Nos.42273042 and 41931077)"From 0 to 1"Original Exploration Cultivation Project,Institute of Geochemistry,Chinese Academy of Sciences(DHSZZ2023-3)Guizhou Provincial Foundation for Excellent Scholars Program(No.GCC[2023]088)Guizhou Provincial Science and Technology Projects:QKHJCZK[2023]-General 473NSFC Young Scientist Fund(Nos.42303041 and 42403043)。
文摘Permanently shadowed regions(PSRs)on the Moon are potential reservoirs for water ice,making them hot spots for future lunar exploration.The water ice in PSRs would cause distinctive changes in space weathering there,in particular reduction-oxidation processes that diff er from those in illuminated regions.To determine the characteristics of products formed during space weathering in PSRs,the lunar meteorite NWA 10203 with artifi cially added water was irradiated with a nanosecond laser to simulate a micro-meteorite bombardment of lunar soil containing water ice.The TEM results of the water-incorporated sample showed distinct amorphous rims that exhibited irregular thickness,poor stratifi cation,the appearance of bubbles,and a reduced number of npFe^(0).Additionally,EELS analysis showed the presence of ferric iron at the rim of the nanophase metallic iron particles(npFe^(0))in the amorphous rim with the involvement of water.The results suggest that water ice is another possible factor contributing to oxidation during micrometeorite bombardment on the lunar surface.In addition,it off ers a reference for a new space weathering model that incorporates water in PSRs,which could be widespread on asteroids with volatiles.
基金supported by grant from the National Key Research and Development Program of China(2021YFC2101402)the National Natural Science Foundation of China(31972064,32302030)the China Postdoctoral Science Foundation(2023M731334)。
文摘Fermented chili products are popular traditional fermented foods around the world.However,differences in microbial communities in fermented chilies from different regions and how they affect the flavor compounds in chili fermentation have not been reported.In this study,the dynamics of flavor compounds and microbial communities in fermented chilies from Sichuan,Guizhou,and Hunan were systematically investigated by macro-genome sequencing,solid phase microextraction-gas chromatograph-mass spectrometry(SPME-GC-MS),electronic nose,and electronic tongue techniques.Simultaneously,the microbial metabolic mechanisms and the relationship between flavor compounds and microbiome were unraveled through staged and simulated fermentation analysis.The results showed that 53 chemical odorants,including alcohols,esters,aldehydes,and acids,were identified as chemical markers to differentiate the regional samples.A total of 12 microbial species,including Staphylococcus xylosus,unclassified Staphylococcus species,Weissella confusus,Lactococcus cremoris,Lactococcus garvieae,Lactiplantibacillus sakei,Pediciococcus propionicigenes,Pediciococcus idahonensis,Pediciococcus aciditolerans,Nocardioides antri,Debaryomyces hansenii,and Colletotrichum scovillei,were identified as microbial markers to differentiate the regional samples.Correlation analysis showed that Lactobacillaceae was associated with fruity,floral,spicy,and fatty aromas.The electronic nose and tongue analysis results showed that 9 flavor and 8 taste indicators significantly differed between regional samples(P<0.05).Additionally,flavor compounds and microbial diversity were robust under initial selection stress and showed higher diversity under metabolome-microbiome interactions.Importantly,simulated fermentation confirmed that metabolome-microbiome interactions drove the shift in microbial structure,metabolism,and flavor in regionally fermented chilies.These results provide insights into the succession of microbial communities and the formation of flavor compounds in chili fermentation,which may enable the future replication of fermented foods with the same flavor.
