Objective Hepatocyte nuclear factor 4-alpha(HNF4A)is a critical transcription factor in the liver and pancreas.Dysfunctions of HNF4A lead to maturity onset diabetes of the young 1(MODY1).Notably,MODY1 patients with HN...Objective Hepatocyte nuclear factor 4-alpha(HNF4A)is a critical transcription factor in the liver and pancreas.Dysfunctions of HNF4A lead to maturity onset diabetes of the young 1(MODY1).Notably,MODY1 patients with HNF4A pathogenic mutations exhibit decreased responses to arginine and reduced plasma triglyceride levels,but the mechanisms remain unclear.This study aims to investigate the potential target genes transcriptionally regulated by HNF4A and explore its role in these metabolic pathways.Methods A stable 293T cell line expressing the HNF1A reporter was overexpressed with HNF4A.RNA sequencing(RNA-seq)was performed to analyze transcriptional differences.Transcription factor binding site prediction was then conducted to identify HNF4A binding motifs in the promoter regions of relevant target genes.Results RNA-seq results revealed a significant upregulation of transmembrane 4 L six family member 5(TM4SF5)mRNA in HNF4A-overexpressing cells.Transcription factor binding predictions suggested the presence of five potential HNF4A binding motifs in the TM4SF5 promoter.Finally,we confirmed that the DR1 site in the-57 to-48 region of the TM4SF5 promoter is the key binding motif for HNF4A.Conclusion This study identified TM4SF5 as a target gene of HNF4A and determined the key binding motif involved in its regulation.Given the role of TM4SF5 as an arginine sensor in mTOR signaling activation and triglyceride secretion,which closely aligns with phenotypes observed in MODY1 patients,our findings provide novel insights into the possible mechanisms by which HNF4A regulates triglyceride secretion in the liver and arginine-stimulated insulin secretion in the pancreas.展开更多
目的探讨血清癌胚抗原相关细胞粘附分子1(carcino-embryonic antigen related cellular adhesion molecule 1,CEACAM1)、四次跨膜蛋白1(transmembrane 4 L six family member 1,TM4SF1)在乳腺癌组织中的表达及诊断价值。方法收集乳腺癌9...目的探讨血清癌胚抗原相关细胞粘附分子1(carcino-embryonic antigen related cellular adhesion molecule 1,CEACAM1)、四次跨膜蛋白1(transmembrane 4 L six family member 1,TM4SF1)在乳腺癌组织中的表达及诊断价值。方法收集乳腺癌96例。采用酶联免疫吸附试验检测血清CEACAM1、TM4SF1水平。统计血清CEACAM1、TM4SF1水平与临床病理参数的关系及诊断效能。结果乳腺癌血清CEACAM1、TM4SF1水平与患者年龄、肿瘤直径、病理类型、雌激素受体、孕激素受体均无关,P>0.05,与病理分化程度、TNM分期、转移情况均有关。血清CEACAM1、TM4SF1水平联合诊断效能均较单一诊断效能高,P<0.05。结论血清CEACAM1、TM4SF1水平与乳腺癌病理分化程度、TNM分期、转移情况均有关,可作为肿瘤诊断标志物,联合诊断效能更高。展开更多
目的:探讨有4个跨膜区的L6超家族成员1(transmembrane 4Lsix family member 1,TM4SF1)基因在上皮性卵巢癌(卵巢癌)组织中的表达及其临床价值。方法:用RT-PCR法检测TM4SF1基因在36例卵巢癌、15例卵巢良性肿瘤、13例正常卵巢组织中的表达...目的:探讨有4个跨膜区的L6超家族成员1(transmembrane 4Lsix family member 1,TM4SF1)基因在上皮性卵巢癌(卵巢癌)组织中的表达及其临床价值。方法:用RT-PCR法检测TM4SF1基因在36例卵巢癌、15例卵巢良性肿瘤、13例正常卵巢组织中的表达。结果:TM4SF1基因在卵巢癌中表达为(0.173±0.054)、良性肿瘤为(0.134±0.015)、正常卵巢组织为(0.118±0.008)。癌组织中的表达水平高于卵巢良性肿瘤(P=0.031);癌组织中的表达水平高于正常卵巢组织(P=0.004);良性卵巢肿瘤组织中的表达水平高于正常卵巢组织(P=0.012);手术—病理分期为Ⅰ-Ⅱ期癌组织中的表达水平(0.124±0.02)低于Ⅲ期(0.164±0.024)(P<0.05);高分化癌组织中的表达水平为(0.091±0.052),中—低分化癌组织表达为(0.113±0.024)(P>0.05)。结论:TM4SF1基因的表达与卵巢癌相关,可能在卵巢癌的发生、发展过程中起一定作用。展开更多
文摘Objective Hepatocyte nuclear factor 4-alpha(HNF4A)is a critical transcription factor in the liver and pancreas.Dysfunctions of HNF4A lead to maturity onset diabetes of the young 1(MODY1).Notably,MODY1 patients with HNF4A pathogenic mutations exhibit decreased responses to arginine and reduced plasma triglyceride levels,but the mechanisms remain unclear.This study aims to investigate the potential target genes transcriptionally regulated by HNF4A and explore its role in these metabolic pathways.Methods A stable 293T cell line expressing the HNF1A reporter was overexpressed with HNF4A.RNA sequencing(RNA-seq)was performed to analyze transcriptional differences.Transcription factor binding site prediction was then conducted to identify HNF4A binding motifs in the promoter regions of relevant target genes.Results RNA-seq results revealed a significant upregulation of transmembrane 4 L six family member 5(TM4SF5)mRNA in HNF4A-overexpressing cells.Transcription factor binding predictions suggested the presence of five potential HNF4A binding motifs in the TM4SF5 promoter.Finally,we confirmed that the DR1 site in the-57 to-48 region of the TM4SF5 promoter is the key binding motif for HNF4A.Conclusion This study identified TM4SF5 as a target gene of HNF4A and determined the key binding motif involved in its regulation.Given the role of TM4SF5 as an arginine sensor in mTOR signaling activation and triglyceride secretion,which closely aligns with phenotypes observed in MODY1 patients,our findings provide novel insights into the possible mechanisms by which HNF4A regulates triglyceride secretion in the liver and arginine-stimulated insulin secretion in the pancreas.
文摘目的探讨血清癌胚抗原相关细胞粘附分子1(carcino-embryonic antigen related cellular adhesion molecule 1,CEACAM1)、四次跨膜蛋白1(transmembrane 4 L six family member 1,TM4SF1)在乳腺癌组织中的表达及诊断价值。方法收集乳腺癌96例。采用酶联免疫吸附试验检测血清CEACAM1、TM4SF1水平。统计血清CEACAM1、TM4SF1水平与临床病理参数的关系及诊断效能。结果乳腺癌血清CEACAM1、TM4SF1水平与患者年龄、肿瘤直径、病理类型、雌激素受体、孕激素受体均无关,P>0.05,与病理分化程度、TNM分期、转移情况均有关。血清CEACAM1、TM4SF1水平联合诊断效能均较单一诊断效能高,P<0.05。结论血清CEACAM1、TM4SF1水平与乳腺癌病理分化程度、TNM分期、转移情况均有关,可作为肿瘤诊断标志物,联合诊断效能更高。
文摘目的:探讨有4个跨膜区的L6超家族成员1(transmembrane 4Lsix family member 1,TM4SF1)基因在上皮性卵巢癌(卵巢癌)组织中的表达及其临床价值。方法:用RT-PCR法检测TM4SF1基因在36例卵巢癌、15例卵巢良性肿瘤、13例正常卵巢组织中的表达。结果:TM4SF1基因在卵巢癌中表达为(0.173±0.054)、良性肿瘤为(0.134±0.015)、正常卵巢组织为(0.118±0.008)。癌组织中的表达水平高于卵巢良性肿瘤(P=0.031);癌组织中的表达水平高于正常卵巢组织(P=0.004);良性卵巢肿瘤组织中的表达水平高于正常卵巢组织(P=0.012);手术—病理分期为Ⅰ-Ⅱ期癌组织中的表达水平(0.124±0.02)低于Ⅲ期(0.164±0.024)(P<0.05);高分化癌组织中的表达水平为(0.091±0.052),中—低分化癌组织表达为(0.113±0.024)(P>0.05)。结论:TM4SF1基因的表达与卵巢癌相关,可能在卵巢癌的发生、发展过程中起一定作用。
