目的探讨TLR-9基因多态性与肺结核易感性的相关性。方法计算机检索知网、万方数据库、大医医学网、PubMed数据库关于TLR-9基因多态性(rs352139和rs187084)与肺结核易感性相关的病例对照研究,检索时间从建库截至2019年10月10日。提取相...目的探讨TLR-9基因多态性与肺结核易感性的相关性。方法计算机检索知网、万方数据库、大医医学网、PubMed数据库关于TLR-9基因多态性(rs352139和rs187084)与肺结核易感性相关的病例对照研究,检索时间从建库截至2019年10月10日。提取相关信息,根据等位基因模型、显性模型、隐性模型、纯合子比较模型、杂合子比较模型进行Meta分析。结果共纳入9篇文献,病例组8 280例、对照组8 959例。Meta分析结果显示:TLR9 rs352139多态性与肺结核易感性无显著相关性,等位基因模型G vs A:OR=1.04,95%CI:0.85~1.27;显性模型AA vs AG+GG:OR=0.91,95%CI:0.71~1.18;隐性模型GG vs AG+AA:OR=1.00,95%CI:0.70~1.44;纯合子比较模型GG vs AA:OR=1.06,95%CI:0.75~1.51;杂合子比较模型AG vs AA:OR=1.09,95%CI:0.82~1.46。TLR9 rs187084多态性与肺结核易感性无显著相关性,等位基因模型C vs T:OR=0.99,95%CI:0.89~1.10;显性模型TT vs TC+CC:OR=1.11,95%CI:0.94~1.31;隐性模型CC vs TC+TT:OR=1.04,95%CI:0.86~1.25;纯合子比较模型CC vs TT:OR=0.92,95%CI:0.72~1.16;杂合子比较模型TC vs TT:OR=0.86,95%CI:0.63~1.17。结论 TLR-9基因多态性(rs352139和rs187084)与肺结核易感性无明显相关性。展开更多
Objective:To investigate the significance of toll receptor-9(TLR-9)expression in rats with acute lung injury(ALI)induced by thoracic trauma.Methods:Fifty healthy male SD rats were selected for the study,and 40 of them...Objective:To investigate the significance of toll receptor-9(TLR-9)expression in rats with acute lung injury(ALI)induced by thoracic trauma.Methods:Fifty healthy male SD rats were selected for the study,and 40 of them were randomly selected to establish the acute lung injury model induced by thoracic trauma,and 8 rats were randomly selected at 5,24,48 and 72 h after the impact,which were killed by cervical dislocation after arterial bleeding,and the lung tissue sections were made.Randomly select 8 rats from the remaining 10 rats as the control group,take blood and kill them to make lung tissue sections for reference.The lung dry/wet weight ratio(W/D)of the five groups were compared,and the levels of TLR-9 in bronchoalveolar lavage fluid(BALF)and serum,and the levels of TLR-9 mR NA,IL-6 mR NA,TLR-9 protein and IL-6protein in lung tissue were detected.Results:There was no significant difference in W/D levels among the control group,experimental group 1 and experimental group 4(P>0.05),while the W/D level of experimental group 3 was higher than that of the other four groups,and the W/D level of experimental group 2 was higher than that of the control group(P<0.05).The levels of ILR-9 in serum and BALF of rats in experimental group 1,experimental group 2 and experimental group 4 had no significant difference(P>0.05),which were higher than those in control group and lower than those in experimental group 3(P<0.05).The levels of TLR-9 mR NA,IL-6 mR NA,TLR-9 protein and IL-6 protein were the highest in the experimental group 3,and the lowest in the control group(P<0.05).The levels of TLR-9 mR NA and IL-6 mR NA in lung tissue of rats in experimental group 1,experimental group 2 and experimental group 4 had no significant difference(P>0.05).There was no significant difference in the levels of TLR-9 protein and IL-6 protein between experimental group 2 and experimental group 4(P>0.05),which were higher than those in experimental group 1(P<0.05).Conclusion:The expression level of TLR-9 is significantly increased in rats after thoracic trauma,and the expression of TLR-9 can reflect the degree of lung injury in rats after trauma to a certain extent,which is inferred to be related to the inflammatory response after lung injury.展开更多
目的：探讨Toll样受体（Tol- like receptors/TLRs）9基因rs187084位点多态性与子宫内膜异位症（EMs）发病风险的关系。方法采用DNA直接测序法检测104例EMs患者（EMs组）和100例非EMs患者（对照组）的TLR9基因rs187084位点的多态性，比...目的：探讨Toll样受体（Tol- like receptors/TLRs）9基因rs187084位点多态性与子宫内膜异位症（EMs）发病风险的关系。方法采用DNA直接测序法检测104例EMs患者（EMs组）和100例非EMs患者（对照组）的TLR9基因rs187084位点的多态性，比较各基因型和等位基因的分布情况。结果 EMs组和对照组中，TLR9基因rs187084位点的AA、AG、GG3种基因型频率分别为42.30%、28.85%、28.85%和52.00%、34.00%、14.00%，两组比较差异有统计学意义（P=0.036）；A、G等位基因频率分别为56.73%、43.27%和69.00%、31.00%，两组比较差异有统计学意义（P=0.01）；携带GG基因型明显增加EMs的发病风险（OR=1.911，95％CI 1.113～3.282）。结论 TLR9基因rs187084位点多态性与EMs的发病风险相关。展开更多
文摘目的探讨TLR-9基因多态性与肺结核易感性的相关性。方法计算机检索知网、万方数据库、大医医学网、PubMed数据库关于TLR-9基因多态性(rs352139和rs187084)与肺结核易感性相关的病例对照研究,检索时间从建库截至2019年10月10日。提取相关信息,根据等位基因模型、显性模型、隐性模型、纯合子比较模型、杂合子比较模型进行Meta分析。结果共纳入9篇文献,病例组8 280例、对照组8 959例。Meta分析结果显示:TLR9 rs352139多态性与肺结核易感性无显著相关性,等位基因模型G vs A:OR=1.04,95%CI:0.85~1.27;显性模型AA vs AG+GG:OR=0.91,95%CI:0.71~1.18;隐性模型GG vs AG+AA:OR=1.00,95%CI:0.70~1.44;纯合子比较模型GG vs AA:OR=1.06,95%CI:0.75~1.51;杂合子比较模型AG vs AA:OR=1.09,95%CI:0.82~1.46。TLR9 rs187084多态性与肺结核易感性无显著相关性,等位基因模型C vs T:OR=0.99,95%CI:0.89~1.10;显性模型TT vs TC+CC:OR=1.11,95%CI:0.94~1.31;隐性模型CC vs TC+TT:OR=1.04,95%CI:0.86~1.25;纯合子比较模型CC vs TT:OR=0.92,95%CI:0.72~1.16;杂合子比较模型TC vs TT:OR=0.86,95%CI:0.63~1.17。结论 TLR-9基因多态性(rs352139和rs187084)与肺结核易感性无明显相关性。
文摘Objective:To investigate the significance of toll receptor-9(TLR-9)expression in rats with acute lung injury(ALI)induced by thoracic trauma.Methods:Fifty healthy male SD rats were selected for the study,and 40 of them were randomly selected to establish the acute lung injury model induced by thoracic trauma,and 8 rats were randomly selected at 5,24,48 and 72 h after the impact,which were killed by cervical dislocation after arterial bleeding,and the lung tissue sections were made.Randomly select 8 rats from the remaining 10 rats as the control group,take blood and kill them to make lung tissue sections for reference.The lung dry/wet weight ratio(W/D)of the five groups were compared,and the levels of TLR-9 in bronchoalveolar lavage fluid(BALF)and serum,and the levels of TLR-9 mR NA,IL-6 mR NA,TLR-9 protein and IL-6protein in lung tissue were detected.Results:There was no significant difference in W/D levels among the control group,experimental group 1 and experimental group 4(P>0.05),while the W/D level of experimental group 3 was higher than that of the other four groups,and the W/D level of experimental group 2 was higher than that of the control group(P<0.05).The levels of ILR-9 in serum and BALF of rats in experimental group 1,experimental group 2 and experimental group 4 had no significant difference(P>0.05),which were higher than those in control group and lower than those in experimental group 3(P<0.05).The levels of TLR-9 mR NA,IL-6 mR NA,TLR-9 protein and IL-6 protein were the highest in the experimental group 3,and the lowest in the control group(P<0.05).The levels of TLR-9 mR NA and IL-6 mR NA in lung tissue of rats in experimental group 1,experimental group 2 and experimental group 4 had no significant difference(P>0.05).There was no significant difference in the levels of TLR-9 protein and IL-6 protein between experimental group 2 and experimental group 4(P>0.05),which were higher than those in experimental group 1(P<0.05).Conclusion:The expression level of TLR-9 is significantly increased in rats after thoracic trauma,and the expression of TLR-9 can reflect the degree of lung injury in rats after trauma to a certain extent,which is inferred to be related to the inflammatory response after lung injury.
