研制了一种新型板式无砟轨道TKT-S(Tie Ke Track-Slab),轨道板采用工厂预制普通混凝土结构;板下为纵向条状聚氨酯支撑层,通过调整聚氨酯层的相关力学指标达到减振目的。在大量分析计算和试验的基础上,确定各部件的构造尺寸与聚氨酯的相...研制了一种新型板式无砟轨道TKT-S(Tie Ke Track-Slab),轨道板采用工厂预制普通混凝土结构;板下为纵向条状聚氨酯支撑层,通过调整聚氨酯层的相关力学指标达到减振目的。在大量分析计算和试验的基础上,确定各部件的构造尺寸与聚氨酯的相关配方等。对减振型和非减振型轨道分别进行了自振频率、模态和谐响应分析,以掌握轨道动力性能。为检验轨道系统的减振性能,在室内分别建立了减振和非减振轨道的实尺模型,开展落锤冲击试验;在中国国家铁道试验中心建立50 m试验段,进行行车试验;同时,对落锤冲击和行车试验进行了仿真计算。计算与试验结果吻合,表明TKT-S具有良好的减振性能。展开更多
目的:观察不同中医治法对肝癌大鼠TKT(转酮酶)基因表达的调控差异,以及TKT基因在人肝癌细胞增殖中的作用。方法:①采用DEN(二乙基亚硝胺)诱发大鼠肝癌,分别经健脾理气、清热解毒、活血化瘀等中药治疗后,Affymetrix rat 230A基因芯片检...目的:观察不同中医治法对肝癌大鼠TKT(转酮酶)基因表达的调控差异,以及TKT基因在人肝癌细胞增殖中的作用。方法:①采用DEN(二乙基亚硝胺)诱发大鼠肝癌,分别经健脾理气、清热解毒、活血化瘀等中药治疗后,Affymetrix rat 230A基因芯片检测大鼠肝癌组织TKT基因表达的差异;②设计3个人TKT基因siRNA(小干扰RNA)序列,将siRNA经脂质体转入SMMC-7721人肝癌细胞株,MTT(噻唑蓝)比色法检测细胞生长增殖情况、RTqPCR(实时荧光定量聚合酶链反应)检测该基因的表达差异。结果:①芯片结果显示,TKT基因在大鼠肝癌形成后表达显著增加,不同中药对其具有不同程度的下调作用,其中健脾理气法下调作用较明显;②采用MTT比色法筛选到1个TKT基因RNAi(RNA干扰)有效靶序列,转染48小时后细胞的生长增殖得到了明显的抑制(与阴性对照组相比,P<0.01);③RTqPCR检测表明,转染48小时后该基因的表达明显降低。结论:TKT基因的高表达与肝癌细胞恶性增殖有关,而中药健脾理气治法对其具有下调作用。展开更多
The pathogenesis of neuroblastoma with bone or bone marrow metastasis (NB-BBM)and its complex immune microenvironment remain poorly elucidated, hampering the advancement of effective risk prediction for BBM and limiti...The pathogenesis of neuroblastoma with bone or bone marrow metastasis (NB-BBM)and its complex immune microenvironment remain poorly elucidated, hampering the advancement of effective risk prediction for BBM and limiting therapeutic strategies. Feature recognition of 142 paraffin-embedded hematoxylin-eosin-stained tumor section images was conductedusing a Swin-Transformer for pathological histology to predict NB-BBM occurrence. Single-celltranscriptomics identified a tumor cell subpopulation (NB3) and two tumor-associated macrophage (TAM) subpopulations (SPP1^(+) TAMs and IGHM^(+) TAMs) closely associated with BBM andhighlighted transketolase (TKT) as a key molecular marker for metastatic progression in NB.This extensive multi-omics investigation into NB-BBM enhances our understanding of single-celltranscriptional dynamics in NB beyond existing research, outlining the evolution from in situcarcinoma through tumorigenesis to bone marrow metastases. Furthermore, exploration ofthe immune microenvironment identified specific subpopulations of TAMs crucial in promotingNB-BBM, presenting new avenues for immunotherapy. These insights enhance our understanding of the metastatic process from NB to BBM and facilitate the development of more effectivediagnostic and therapeutic strategies for this aggressive pediatric cancer.展开更多
文摘研制了一种新型板式无砟轨道TKT-S(Tie Ke Track-Slab),轨道板采用工厂预制普通混凝土结构;板下为纵向条状聚氨酯支撑层,通过调整聚氨酯层的相关力学指标达到减振目的。在大量分析计算和试验的基础上,确定各部件的构造尺寸与聚氨酯的相关配方等。对减振型和非减振型轨道分别进行了自振频率、模态和谐响应分析,以掌握轨道动力性能。为检验轨道系统的减振性能,在室内分别建立了减振和非减振轨道的实尺模型,开展落锤冲击试验;在中国国家铁道试验中心建立50 m试验段,进行行车试验;同时,对落锤冲击和行车试验进行了仿真计算。计算与试验结果吻合,表明TKT-S具有良好的减振性能。
文摘目的:观察不同中医治法对肝癌大鼠TKT(转酮酶)基因表达的调控差异,以及TKT基因在人肝癌细胞增殖中的作用。方法:①采用DEN(二乙基亚硝胺)诱发大鼠肝癌,分别经健脾理气、清热解毒、活血化瘀等中药治疗后,Affymetrix rat 230A基因芯片检测大鼠肝癌组织TKT基因表达的差异;②设计3个人TKT基因siRNA(小干扰RNA)序列,将siRNA经脂质体转入SMMC-7721人肝癌细胞株,MTT(噻唑蓝)比色法检测细胞生长增殖情况、RTqPCR(实时荧光定量聚合酶链反应)检测该基因的表达差异。结果:①芯片结果显示,TKT基因在大鼠肝癌形成后表达显著增加,不同中药对其具有不同程度的下调作用,其中健脾理气法下调作用较明显;②采用MTT比色法筛选到1个TKT基因RNAi(RNA干扰)有效靶序列,转染48小时后细胞的生长增殖得到了明显的抑制(与阴性对照组相比,P<0.01);③RTqPCR检测表明,转染48小时后该基因的表达明显降低。结论:TKT基因的高表达与肝癌细胞恶性增殖有关,而中药健脾理气治法对其具有下调作用。
基金supported in part by research grants from the Key Project of the National Key R&D Plan“Research on Prevention and Control of Major Chronic Non-Communicable Diseases”(China)the Ministry of Science and Technology of the People’s Republic of China,and the National Key R&D Program of China(No.2018YFC1313000,2018YFC1313004).
文摘The pathogenesis of neuroblastoma with bone or bone marrow metastasis (NB-BBM)and its complex immune microenvironment remain poorly elucidated, hampering the advancement of effective risk prediction for BBM and limiting therapeutic strategies. Feature recognition of 142 paraffin-embedded hematoxylin-eosin-stained tumor section images was conductedusing a Swin-Transformer for pathological histology to predict NB-BBM occurrence. Single-celltranscriptomics identified a tumor cell subpopulation (NB3) and two tumor-associated macrophage (TAM) subpopulations (SPP1^(+) TAMs and IGHM^(+) TAMs) closely associated with BBM andhighlighted transketolase (TKT) as a key molecular marker for metastatic progression in NB.This extensive multi-omics investigation into NB-BBM enhances our understanding of single-celltranscriptional dynamics in NB beyond existing research, outlining the evolution from in situcarcinoma through tumorigenesis to bone marrow metastases. Furthermore, exploration ofthe immune microenvironment identified specific subpopulations of TAMs crucial in promotingNB-BBM, presenting new avenues for immunotherapy. These insights enhance our understanding of the metastatic process from NB to BBM and facilitate the development of more effectivediagnostic and therapeutic strategies for this aggressive pediatric cancer.
