慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD,慢阻肺)是一种由气道炎症和气道重塑(airway remodeling, AR)引起的一种常见的慢性呼吸系统疾病。其中,气道重塑与COPD的进展之间存在的正反馈环显示了气道重塑在COPD进...慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD,慢阻肺)是一种由气道炎症和气道重塑(airway remodeling, AR)引起的一种常见的慢性呼吸系统疾病。其中,气道重塑与COPD的进展之间存在的正反馈环显示了气道重塑在COPD进展中的重要性。目前气道重塑的具体机制不明。临床上通过胸部CT发现的气道异常常提示患者已经发生了气道重塑,因此,寻找识别早期气道重塑的方法至关重要。研究表明,基质金属蛋白酶-9 (matrix metalloproteinase-9, MMP-9)、金属蛋白酶组织抑制剂-1 (tissue inhibitor of metalloproteinase-1, TIMP-1)、及MMP-9/TIMP-1比值之间的失衡参与COPD的气道重塑过程,本文叙述了MMP-9、TIMP-1及MMP-9/TIMP-1比值作为非侵入性诊断标志物在COPD气道重塑方面的研究进展,期待能提高人们对早期气道重塑的认知,希望能为将来气道重塑靶向药的问世尽一份绵薄之力。Chronic obstructive pulmonary disease (COPD) is a common chronic respiratory disease caused by airway inflammation and airway remodeling (AR). Among them, the positive feedback loop between airway remodeling and the progression of COPD shows the importance of airway remodeling in the progress of COPD. At present, the specific mechanism of airway remodeling is unknown. Airway abnormalities found by chest CT often indicate that airway remodeling has occurred in patients. Therefore, it is very important to find a method to identify early airway remodeling. Studies have shown that the imbalance between matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) and MMP-9/TIMP-1 ratio is involved in the airway remodeling of COPD. This paper describes the research progress of MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio as non-invasive diagnostic markers in airway remodeling of COPD. It is expected to improve people’s understanding of early airway remodeling and make a modest contribution to the development of targeted drugs for airway remodeling in the future.展开更多
目的观察苦参碱对单侧输尿管梗阻(UUO)大鼠模型肾小管间质基质金属蛋白酶-3(MM P-3)、基质金属蛋白酶组织抑制物-1(T IM P-1)和纤维联结蛋白(FN)表达水平的影响,初步探讨其对小管间质纤维化的作用机制。方法实验采用UUO肾间质纤维化模...目的观察苦参碱对单侧输尿管梗阻(UUO)大鼠模型肾小管间质基质金属蛋白酶-3(MM P-3)、基质金属蛋白酶组织抑制物-1(T IM P-1)和纤维联结蛋白(FN)表达水平的影响,初步探讨其对小管间质纤维化的作用机制。方法实验采用UUO肾间质纤维化模型作为研究模型,分为正常组、假UUO组、UUO组、福辛普利治疗组(F组)、苦参碱大剂量治疗组(E组)和苦参碱小剂量治疗组(D组)。运用免疫组化技术半定量分析实验第7、14 d各组的MM P-3、T IM P-1和FN表达水平。结果UUO组和治疗组的MM P-3表达水平第7、14 d均低于正常组和假UUO组(P<0.05),但三个治疗组的表达水平高于UUO组(P<0.05);T IM P-1和FN在正常组和假UUO组中的表达最低,而三个治疗组的表达均低于UUO组(P<0.05);MM P-3、T IM P-1和FN在E组与F组间的表达差异均无统计学意义(P>0.05);在UUO组中,MM P-3与FN、MM P-3与T IM P-1的免疫组化半定量分析均呈负相关(P<0.01)。结论苦参碱可以部分恢复小管间质MM P-3的表达水平,降低T IM P-1和FN的表达水平,从而延缓肾小管间质纤维化的进程。展开更多
文摘慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD,慢阻肺)是一种由气道炎症和气道重塑(airway remodeling, AR)引起的一种常见的慢性呼吸系统疾病。其中,气道重塑与COPD的进展之间存在的正反馈环显示了气道重塑在COPD进展中的重要性。目前气道重塑的具体机制不明。临床上通过胸部CT发现的气道异常常提示患者已经发生了气道重塑,因此,寻找识别早期气道重塑的方法至关重要。研究表明,基质金属蛋白酶-9 (matrix metalloproteinase-9, MMP-9)、金属蛋白酶组织抑制剂-1 (tissue inhibitor of metalloproteinase-1, TIMP-1)、及MMP-9/TIMP-1比值之间的失衡参与COPD的气道重塑过程,本文叙述了MMP-9、TIMP-1及MMP-9/TIMP-1比值作为非侵入性诊断标志物在COPD气道重塑方面的研究进展,期待能提高人们对早期气道重塑的认知,希望能为将来气道重塑靶向药的问世尽一份绵薄之力。Chronic obstructive pulmonary disease (COPD) is a common chronic respiratory disease caused by airway inflammation and airway remodeling (AR). Among them, the positive feedback loop between airway remodeling and the progression of COPD shows the importance of airway remodeling in the progress of COPD. At present, the specific mechanism of airway remodeling is unknown. Airway abnormalities found by chest CT often indicate that airway remodeling has occurred in patients. Therefore, it is very important to find a method to identify early airway remodeling. Studies have shown that the imbalance between matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) and MMP-9/TIMP-1 ratio is involved in the airway remodeling of COPD. This paper describes the research progress of MMP-9, TIMP-1 and MMP-9/TIMP-1 ratio as non-invasive diagnostic markers in airway remodeling of COPD. It is expected to improve people’s understanding of early airway remodeling and make a modest contribution to the development of targeted drugs for airway remodeling in the future.
文摘目的观察苦参碱对单侧输尿管梗阻(UUO)大鼠模型肾小管间质基质金属蛋白酶-3(MM P-3)、基质金属蛋白酶组织抑制物-1(T IM P-1)和纤维联结蛋白(FN)表达水平的影响,初步探讨其对小管间质纤维化的作用机制。方法实验采用UUO肾间质纤维化模型作为研究模型,分为正常组、假UUO组、UUO组、福辛普利治疗组(F组)、苦参碱大剂量治疗组(E组)和苦参碱小剂量治疗组(D组)。运用免疫组化技术半定量分析实验第7、14 d各组的MM P-3、T IM P-1和FN表达水平。结果UUO组和治疗组的MM P-3表达水平第7、14 d均低于正常组和假UUO组(P<0.05),但三个治疗组的表达水平高于UUO组(P<0.05);T IM P-1和FN在正常组和假UUO组中的表达最低,而三个治疗组的表达均低于UUO组(P<0.05);MM P-3、T IM P-1和FN在E组与F组间的表达差异均无统计学意义(P>0.05);在UUO组中,MM P-3与FN、MM P-3与T IM P-1的免疫组化半定量分析均呈负相关(P<0.01)。结论苦参碱可以部分恢复小管间质MM P-3的表达水平,降低T IM P-1和FN的表达水平,从而延缓肾小管间质纤维化的进程。
