目的探讨结直肠癌(colorectal cancer,CRC)组织中T细胞免疫球蛋白黏蛋白3(T cell immunoglobulin and mucin domain-containing protein 3,TIM3)、E3泛素蛋白连接酶-1(seven in absentia homolog-1,SIAH1)的表达特征及其与预后的关系。...目的探讨结直肠癌(colorectal cancer,CRC)组织中T细胞免疫球蛋白黏蛋白3(T cell immunoglobulin and mucin domain-containing protein 3,TIM3)、E3泛素蛋白连接酶-1(seven in absentia homolog-1,SIAH1)的表达特征及其与预后的关系。方法选取2019年6月―2022年3月在河北北方学院附属第二医院住院手术的CRC患者148例为研究对象,收集整理CRC患者临床资料,免疫组化法检测TIM3、SIAH1蛋白表达;Spearman法分析CRC组织TIM3与SIAH1表达之间的相关性;CRC组织TIM3、SIAH1与患者预后关系用Kaplan-Meier法分析;Cox回归分析影响预后的因素。结果UALCAN数据库中,癌组织中TIM3阳性表达率(53.19%)高于正常组织(15.79%),癌组织中SIAH1阳性表达率(33.64%)低于正常组织(60.71%)(P<0.05);免疫组织化学检测结果中,TIM3阳性表达率(70.95%)高于癌旁组织(38.51%),SIAH1阳性表达率(33.11%)低于癌旁组织(62.16%),差异有统计学意义(P<0.05);TIM3、SIAH1表达与CRC患者分化程度、临床分期以及淋巴结转移均有关(P<0.05);Spearman相关分析得出,CRC组织TIM3、SIAH1表达呈负相关(r=―0.435,P<0.05);CRC组织TIM3阳性表达患者3年生存率(54/105,51.43%)低于TIM3阴性表达患者(37/43,86.05%)(Log Rankχ^(2)=15.183,P<0.001);TIM3阳性组中位生存期为28.124个月(95%CI:26.220~30.028),短于TIM3阴性组的33.628个月(95%CI:31.818~35.437);CRC组织SIAH1阳性表达患者3年生存率(43/49,87.76%)高于SIAH1阴性表达患者(48/99,48.48%)(Log Rankχ^(2)=21.016,P<0.001),SIAH1阳性组中位生存期为34.76个月(95%CI:33.644~35.907)长于SIAH1阴性组的27.222个月(95%CI:25.219~29.225);分化程度(HR=2.457,95%CI:1.133~5.329)、临床分期(HR=2.861,95%CI:1.364~6.002)淋巴结转移(HR=2.729,95%CI:1.382~5.387)以及TIM3(HR=3.562,95%CI:1.833~6.922)、SIAH1(HR=0.613,95%CI:0.470~0.799)是影响预后的因素(P<0.05)。结论TIM3、SIAH1在CRC组织中呈现不同的表达特征,且其表达水平与患者预后有关。展开更多
目的探讨T细胞免疫球蛋白黏蛋白分子(Tim-3)及半乳糖凝集素-9(Galectin-9)的表达与肿瘤T细胞免疫在鼻咽癌中的临床意义。方法在癌症基因组图谱计划(The Cancer Genome Atlas,TCGA)数据库中查找Tim-3在各种肿瘤组织中的表达情况,选取与...目的探讨T细胞免疫球蛋白黏蛋白分子(Tim-3)及半乳糖凝集素-9(Galectin-9)的表达与肿瘤T细胞免疫在鼻咽癌中的临床意义。方法在癌症基因组图谱计划(The Cancer Genome Atlas,TCGA)数据库中查找Tim-3在各种肿瘤组织中的表达情况,选取与鼻咽癌患者免疫密切相关的基因Tim-3及其配体Galectin-9作为研究基因;收集2018年3月~2022年3月广西壮族自治区民族医院收治的65例鼻咽癌患者的肿瘤组织、同期65例鼻咽部炎性标本组织及对应鼻咽癌患者放疗前后应用免疫细胞病理芯片技术检测的外周血T细胞亚群变化的数据,并做随访观察。结果生物信息学分析TCGA数据库中鼻咽癌的测序数据,结果显示,Tim-3在大部分肿瘤组织中呈高表达,在鼻咽癌组织中较癌旁正常组织表达水平显著上调,Tim-3及其配体Galectin-9在癌组织中的表达呈正相关;免疫组化结果显示,鼻咽癌组织中Tim-3和Galectin-9的表达水平均显著高于鼻咽部炎性组织(P<0.05);鼻咽癌组织中Tim-3和Galectin-9高表达与淋巴结受累(P<0.05)以及更晚的TNM分期(P<0.05)紧密相关;Tim-3和Galectin-9的表达水平与患者年龄、性别及EB病毒感染等因素无关(P>0.05);Tim-3和Galectin-9在鼻咽癌中的表达呈正相关(r=0.313,P=0.011);随访发现,Tim-3和Galectin-9的高表达可能与鼻咽癌的不良预后相关;鼻咽癌患者放疗前3天和放疗后7天的T淋巴细胞亚群检测显示,CD4+、CD4+/CD8+较放疗前显著下降(P<0.05),CD3+与CD8+变化差异无统计学意义(P>0.05);Tim-3和Galectin-9蛋白阳性组患者放疗敏感度明显高于于Tim-3和Galectin-9阴性组(P<0.05)。结论Tim-3/Galectin-9信号通路在鼻咽癌发生、发展和转移中起重要作用,T细胞免疫在鼻咽癌治疗中发挥重要作用。联合检测鼻咽癌组织中Tim-3和Galectin-9及T细胞亚群的变化可能为临床评估肿瘤监测病情及肿瘤免疫治疗提供新参数。展开更多
Currently,the BeiDou⁃3(BDS⁃3)precise point positioning(PPP)service(PPP⁃B2b)mostly employs the ionosphere⁃free(IF)combination model for precise timing,which tends to amplify the noise in observation values.To address t...Currently,the BeiDou⁃3(BDS⁃3)precise point positioning(PPP)service(PPP⁃B2b)mostly employs the ionosphere⁃free(IF)combination model for precise timing,which tends to amplify the noise in observation values.To address this issue,this paper proposes a real⁃time BDS⁃3 precise unidirectional timing model based on uncombined(UC)observations using the BDS⁃3 PPP⁃B2b service.This model resolves the challenge of the amplified observation noise inherent in the IF combination model.The experiment involved selecting eight global navigation satellite system(GNSS)observation stations within China and collecting continuous observation data for 15 d.A comparative analy⁃sis with the traditional dual⁃frequency IF combination PPP timing model showed that the BDS⁃3 UC PPP timing based on the BDS⁃3 PPP⁃B2b service can achieve a timing preci⁃sion of 0.5 ns.In addition,it was found that due to global positioning system(GPS)satellite clock products in the BDS⁃3 PPP⁃B2b service not being unified to the standard time,the GPS IF PPP timing method based on the BDS⁃3 PPP⁃B2b service is not recommended for precise timing.In summary,the BDS⁃3 UC PPP timing model proposed in this paper is suitable for precise timing,providing observa⁃tion values with smaller noise,and its timing accuracy is comparable to that of the BDS⁃3 IF PPP,with slightly better frequency stability.展开更多
文摘目的探讨结直肠癌(colorectal cancer,CRC)组织中T细胞免疫球蛋白黏蛋白3(T cell immunoglobulin and mucin domain-containing protein 3,TIM3)、E3泛素蛋白连接酶-1(seven in absentia homolog-1,SIAH1)的表达特征及其与预后的关系。方法选取2019年6月―2022年3月在河北北方学院附属第二医院住院手术的CRC患者148例为研究对象,收集整理CRC患者临床资料,免疫组化法检测TIM3、SIAH1蛋白表达;Spearman法分析CRC组织TIM3与SIAH1表达之间的相关性;CRC组织TIM3、SIAH1与患者预后关系用Kaplan-Meier法分析;Cox回归分析影响预后的因素。结果UALCAN数据库中,癌组织中TIM3阳性表达率(53.19%)高于正常组织(15.79%),癌组织中SIAH1阳性表达率(33.64%)低于正常组织(60.71%)(P<0.05);免疫组织化学检测结果中,TIM3阳性表达率(70.95%)高于癌旁组织(38.51%),SIAH1阳性表达率(33.11%)低于癌旁组织(62.16%),差异有统计学意义(P<0.05);TIM3、SIAH1表达与CRC患者分化程度、临床分期以及淋巴结转移均有关(P<0.05);Spearman相关分析得出,CRC组织TIM3、SIAH1表达呈负相关(r=―0.435,P<0.05);CRC组织TIM3阳性表达患者3年生存率(54/105,51.43%)低于TIM3阴性表达患者(37/43,86.05%)(Log Rankχ^(2)=15.183,P<0.001);TIM3阳性组中位生存期为28.124个月(95%CI:26.220~30.028),短于TIM3阴性组的33.628个月(95%CI:31.818~35.437);CRC组织SIAH1阳性表达患者3年生存率(43/49,87.76%)高于SIAH1阴性表达患者(48/99,48.48%)(Log Rankχ^(2)=21.016,P<0.001),SIAH1阳性组中位生存期为34.76个月(95%CI:33.644~35.907)长于SIAH1阴性组的27.222个月(95%CI:25.219~29.225);分化程度(HR=2.457,95%CI:1.133~5.329)、临床分期(HR=2.861,95%CI:1.364~6.002)淋巴结转移(HR=2.729,95%CI:1.382~5.387)以及TIM3(HR=3.562,95%CI:1.833~6.922)、SIAH1(HR=0.613,95%CI:0.470~0.799)是影响预后的因素(P<0.05)。结论TIM3、SIAH1在CRC组织中呈现不同的表达特征,且其表达水平与患者预后有关。
文摘目的探讨T细胞免疫球蛋白黏蛋白分子(Tim-3)及半乳糖凝集素-9(Galectin-9)的表达与肿瘤T细胞免疫在鼻咽癌中的临床意义。方法在癌症基因组图谱计划(The Cancer Genome Atlas,TCGA)数据库中查找Tim-3在各种肿瘤组织中的表达情况,选取与鼻咽癌患者免疫密切相关的基因Tim-3及其配体Galectin-9作为研究基因;收集2018年3月~2022年3月广西壮族自治区民族医院收治的65例鼻咽癌患者的肿瘤组织、同期65例鼻咽部炎性标本组织及对应鼻咽癌患者放疗前后应用免疫细胞病理芯片技术检测的外周血T细胞亚群变化的数据,并做随访观察。结果生物信息学分析TCGA数据库中鼻咽癌的测序数据,结果显示,Tim-3在大部分肿瘤组织中呈高表达,在鼻咽癌组织中较癌旁正常组织表达水平显著上调,Tim-3及其配体Galectin-9在癌组织中的表达呈正相关;免疫组化结果显示,鼻咽癌组织中Tim-3和Galectin-9的表达水平均显著高于鼻咽部炎性组织(P<0.05);鼻咽癌组织中Tim-3和Galectin-9高表达与淋巴结受累(P<0.05)以及更晚的TNM分期(P<0.05)紧密相关;Tim-3和Galectin-9的表达水平与患者年龄、性别及EB病毒感染等因素无关(P>0.05);Tim-3和Galectin-9在鼻咽癌中的表达呈正相关(r=0.313,P=0.011);随访发现,Tim-3和Galectin-9的高表达可能与鼻咽癌的不良预后相关;鼻咽癌患者放疗前3天和放疗后7天的T淋巴细胞亚群检测显示,CD4+、CD4+/CD8+较放疗前显著下降(P<0.05),CD3+与CD8+变化差异无统计学意义(P>0.05);Tim-3和Galectin-9蛋白阳性组患者放疗敏感度明显高于于Tim-3和Galectin-9阴性组(P<0.05)。结论Tim-3/Galectin-9信号通路在鼻咽癌发生、发展和转移中起重要作用,T细胞免疫在鼻咽癌治疗中发挥重要作用。联合检测鼻咽癌组织中Tim-3和Galectin-9及T细胞亚群的变化可能为临床评估肿瘤监测病情及肿瘤免疫治疗提供新参数。
基金The Basic Science Center Project of the National Natural Science Foundation of China(No.42388102)the Jiangsu Province Natural Resources Science and Technology Project(No.JSZRKJ202404).
文摘Currently,the BeiDou⁃3(BDS⁃3)precise point positioning(PPP)service(PPP⁃B2b)mostly employs the ionosphere⁃free(IF)combination model for precise timing,which tends to amplify the noise in observation values.To address this issue,this paper proposes a real⁃time BDS⁃3 precise unidirectional timing model based on uncombined(UC)observations using the BDS⁃3 PPP⁃B2b service.This model resolves the challenge of the amplified observation noise inherent in the IF combination model.The experiment involved selecting eight global navigation satellite system(GNSS)observation stations within China and collecting continuous observation data for 15 d.A comparative analy⁃sis with the traditional dual⁃frequency IF combination PPP timing model showed that the BDS⁃3 UC PPP timing based on the BDS⁃3 PPP⁃B2b service can achieve a timing preci⁃sion of 0.5 ns.In addition,it was found that due to global positioning system(GPS)satellite clock products in the BDS⁃3 PPP⁃B2b service not being unified to the standard time,the GPS IF PPP timing method based on the BDS⁃3 PPP⁃B2b service is not recommended for precise timing.In summary,the BDS⁃3 UC PPP timing model proposed in this paper is suitable for precise timing,providing observa⁃tion values with smaller noise,and its timing accuracy is comparable to that of the BDS⁃3 IF PPP,with slightly better frequency stability.
