Based on the program developed to simulate the temperature field for ISM(Induction Skull Melting) process, the effects of power increasing rate and charge mass on the melting throughout time and the energy (consumptio...Based on the program developed to simulate the temperature field for ISM(Induction Skull Melting) process, the effects of power increasing rate and charge mass on the melting throughout time and the energy (consumption) for TiAl alloys were studied. The results show that the melting throughout time decreases (exponentially) with the increasing of the power increasing rate and it linearly increases with the increasing of the charge mass. There is a critical power increasing rate for different charge masses. At this critical power increasing rate, the charge can be molten when the melting power just reaches 300 kW. There exists an optimal power increasing rate, i.e. 1.3 kW/s. At the optimal power increasing rate, the energy consumption for melting the charge is the minimum. The charge mass has noticeable influence on the energy consumed by unit mass and the ratio of effective energy.展开更多
Objective To develop a simple,cheap,quick,accurate and practical method for a high throughout genotypes assay of human papillomavirus (HPV) DNA. Methods Crude DNA was extracted by a simplified proteinase K digesting...Objective To develop a simple,cheap,quick,accurate and practical method for a high throughout genotypes assay of human papillomavirus (HPV) DNA. Methods Crude DNA was extracted by a simplified proteinase K digesting method. HPV common conservative primers: GP5+/6+ system was used to amplify HPV DNA in 127 samples of condylomata acuminatum (CA) and cervical scrapes by PCR,then the PCR product was assayed using a template directing terminator incorporation (TDI) and genotypes were detected with fluorescence polarization (FP). Major HPVs type-specific probes (HPV6,11,16,18,31,33,35 and 58) designed by us were hybridized with the specific PCR products and a special fluorescent ddNTP terminator was directly added to the end of the probe under direction of specific PCR products. The results were measured with FP and compared with the results of the DNA sequence. Results Compared with the results of DNA sequencing,the results detected with fluorescence polarization were all correct. The proposed method could detect more than one type of HPV infection,but DNA sequencing method could not. The positive rate of HPV was 100% in 78 CA biopsies. Among them,there were 14 HPV double infections [HPV6B and 11 (9 cases),HPV11 and 16 (4),HPV11 and 18 (1)],5 HPV triple infections [HPV6B,11 and 16 (4),HPV11,16 and 18 (1)],and one HPV quadruple infection (HPV6B,11,16 and 18). The positive rate of HPV was 77% in the 49 cervical scrapes. Six HPV double infections [HPV6B and 11 (2),HPV11 and 16 (1),HPV6B and 16 (1),HPV16 and 18 (1),HPV18 and 58 (1)], 3 HPV triple infections [HPV6B,11 and 16 (2),HPV11,16 and 18 (1)] and one HPV quadruple infection (HPV6B,11,16 and 18) were detected in cervical cancer scrapes.Conclusions The proposed method allowed a high throughout,special,simple,rapid,automatic and economical detection of HPV-DNA genotyping without a use of labeling probes. It can detect multiple HPV genotype infection and will be and useful tool in HPV genotype screening.展开更多
物联网(Internet of Things,IoT)与5G网络不断部署,边缘传感设备计算负载与持续供能需求显著增加。无线供能移动边缘计算(Wireless-Powered Mobile Edge Computing,WP-MEC)技术通过将无线能量传输(Wireless Power Transfer,WPT)与移动...物联网(Internet of Things,IoT)与5G网络不断部署,边缘传感设备计算负载与持续供能需求显著增加。无线供能移动边缘计算(Wireless-Powered Mobile Edge Computing,WP-MEC)技术通过将无线能量传输(Wireless Power Transfer,WPT)与移动边缘计算(Wireless Edge Computing,MEC)相结合,为延长边缘设备的供电寿命、提高系统整体计算能力提供了新途径。然而,前人的工作主要聚焦于单时隙资源优化或单小区网络模型,资源利用效率不高、与实际偏差较大。为此,聚焦基于非正交多址接入(Non-Orthogonal Multiple Access,NOMA)的多小区WP-MEC网络多时隙优化设计,通过联合优化能量传输时间、任务卸载策略与功率分配,充分利用能量累积增益,最大化系统长时吞吐量。为实现复杂动态环境下的高效资源调度,提出了一种异构多智能体近端策略优化算法(Heterogeneous Multi-Agent Proximal Policy Optimization,HMAPPO),采用全局控制智能体与设备智能体的分层结构,实现了全局能量传输时间与局部任务卸载、功率分配的协同优化。与基于价值函数更新网络的多智能体软演员-评论家(Multi-Agent Soft Actor-Critic,MASAC)和多智能体双延迟深度确定性策略梯度(Multi-Agent Twin Delayed Deep Deterministic Policy Gradient,MATD3)算法不同,HMAPPO采用了限制新旧策略变化幅度的近端策略优化更新机制,更适合多时隙能量的动态连续动作空间,因此在WP-MEC网络环境中具备更高的训练稳定性。仿真结果表明,所提HMAPPO算法在实现了分布式优化的情况下,达到了与集中式近端策略优化(Proximal Policy Optimization,PPO)相接近的性能,性能差别不到3.3%。此外,HMAPPO在不同小区数、设备数及设备距离条件下表现良好,验证了其优良的泛化性和可扩展性。展开更多
基金Project(50271020 50395102) supported by the National Natural Science Foundation of China Project(2002AA305209)supported by Hi tech Research and Development Program of China
文摘Based on the program developed to simulate the temperature field for ISM(Induction Skull Melting) process, the effects of power increasing rate and charge mass on the melting throughout time and the energy (consumption) for TiAl alloys were studied. The results show that the melting throughout time decreases (exponentially) with the increasing of the power increasing rate and it linearly increases with the increasing of the charge mass. There is a critical power increasing rate for different charge masses. At this critical power increasing rate, the charge can be molten when the melting power just reaches 300 kW. There exists an optimal power increasing rate, i.e. 1.3 kW/s. At the optimal power increasing rate, the energy consumption for melting the charge is the minimum. The charge mass has noticeable influence on the energy consumed by unit mass and the ratio of effective energy.
文摘Objective To develop a simple,cheap,quick,accurate and practical method for a high throughout genotypes assay of human papillomavirus (HPV) DNA. Methods Crude DNA was extracted by a simplified proteinase K digesting method. HPV common conservative primers: GP5+/6+ system was used to amplify HPV DNA in 127 samples of condylomata acuminatum (CA) and cervical scrapes by PCR,then the PCR product was assayed using a template directing terminator incorporation (TDI) and genotypes were detected with fluorescence polarization (FP). Major HPVs type-specific probes (HPV6,11,16,18,31,33,35 and 58) designed by us were hybridized with the specific PCR products and a special fluorescent ddNTP terminator was directly added to the end of the probe under direction of specific PCR products. The results were measured with FP and compared with the results of the DNA sequence. Results Compared with the results of DNA sequencing,the results detected with fluorescence polarization were all correct. The proposed method could detect more than one type of HPV infection,but DNA sequencing method could not. The positive rate of HPV was 100% in 78 CA biopsies. Among them,there were 14 HPV double infections [HPV6B and 11 (9 cases),HPV11 and 16 (4),HPV11 and 18 (1)],5 HPV triple infections [HPV6B,11 and 16 (4),HPV11,16 and 18 (1)],and one HPV quadruple infection (HPV6B,11,16 and 18). The positive rate of HPV was 77% in the 49 cervical scrapes. Six HPV double infections [HPV6B and 11 (2),HPV11 and 16 (1),HPV6B and 16 (1),HPV16 and 18 (1),HPV18 and 58 (1)], 3 HPV triple infections [HPV6B,11 and 16 (2),HPV11,16 and 18 (1)] and one HPV quadruple infection (HPV6B,11,16 and 18) were detected in cervical cancer scrapes.Conclusions The proposed method allowed a high throughout,special,simple,rapid,automatic and economical detection of HPV-DNA genotyping without a use of labeling probes. It can detect multiple HPV genotype infection and will be and useful tool in HPV genotype screening.
