In recent years,the prevalence of rice sheath blight caused by Rhizoctonia solani has significantly increased in Heilongjiang Province.Chemical control has become the primary control method.To cope with this,a novel m...In recent years,the prevalence of rice sheath blight caused by Rhizoctonia solani has significantly increased in Heilongjiang Province.Chemical control has become the primary control method.To cope with this,a novel mycelium growth rate method was employed to assess the toxicity of 13 fungicides,including a combination of 45%prochloraz and 125 g·mL^(-1)epoxiconazole,against R.solani.Additionally,the resistance of 99 R.solani strains to thifluzamide across various regions was also evaluated.The findings indicated that 75%trifloxystrobin-tebuconazole exhibited the most effective inhibitory effect,with an effective inhibitory medium concentration(EC50)value of 0.0101μg·mL^(-1).The EC50 values for 20%prothioconazole,125 g·mL^(-1)epoxiconazole,24%thifluzamide,and 50%hexaconazole were all less than 10μg·mL^(-1),indicating a better inhibitory effect on R.solani.The strongest synergistic effect was noted in the mixture of prochloraz and epoxiconazole at a 1:2 ratio,resulting in an EC50 value of 2.9917μg·mL^(-1),and a co-toxicity coefficient of 213.38.Among the 34 strains from Harbin City,the average EC50 value was 196.9341μg·mL^(-1)indicating the highest susceptiblility to thifluzamide.Conversely,15 strains from Shuangyashan City exhibited an average EC50 value of 364.7323μg·mL^(-1),reflecting the lowest sensitivity to thifluzamide.The sensitivity baseline EC50 value for R.solani was 253.8854μg·mL^(-1),with an overall resistance level between 0.1567 and 3.3292,indicating that the resistance level of R.solani in Heilongjiang Province remained low.Therefore,R.solani was still sensitive to thifluzamide in most areas of Heilongjiang Province,but there was a certain risk of resistance in Qitaihe City,which needed to be continuously monitored.At the same time,this study might provide a theoretical foundation for enhancing the prevention and management of the rice sheath blight.展开更多
[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The contr...[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The control efficacy of 16% validamycin·thifluzamide SC against S.rolfsii was determined by spraying agents on the whole plant of D.officinale. The significance of differences was analyzed by Duncan＇s new multiple range test （DMRT）. [Result] The control efficacy of 16% validamycin.thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg was 85.33% at 14 d post spraying, higher than that of control agent 4% validamycin AS sprayed at the dose of 80 mg a.i./kg （78.36%）, and equivalent to that of control agent 240 g/L thifluzamide SC sprayed at the dose of 120 mg a.i./kg （90.60%）. [Conclusion] 16% Validamycin·thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg could effectively control the damage of S.rolfsii in D.officinale, and could be popularized in the production.展开更多
In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statisti...In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statistical analysis were conducted to study the control effect of biopesticide chitosan, biochemical compound Prochloraz-Chitosan and chemical pesticides difenoconazole and thifluzamide by field efficacy trials. The results showed that under serious occurrence of anthracnose in autumn greenhouse cowpea, when the 2% chJtosan AS （2 250 g/hm^2）, 46% Prochloraz-Chitosan EW （450 g/hm^2）, 10% difenoconazole WG （900 g/hm^2, CK） or 24% thifluzamide SC （360 ml/hm^2） were applied three times with an interval of 5-8 d, the control efficiency on day 7, 14 and 20 reached 83%, 78% and 73%, respectively. No significant difference was found in control efficiency among the four kinds of agents. These four kinds of agents, especially chitosan and Prochloraz-Chitosan, could be used as ideal agents for controlling anthracnose, as well as for modern agricultural demonstration zones, pollution-free agricultural products, green agricultural products and organic agricultural products,thereby meeting the develop- ment needs of ＇modern agriculture, green agriculture, organic agriculture and ecological agriculture.展开更多
基金Supported by the Green Plant Protection Project(213010801)the Heilongjiang Province Key Research and Development Plan Project(20232X02 B0502)the Natural Science Foundation of Heilongjiang Province(LH2022C022)。
文摘In recent years,the prevalence of rice sheath blight caused by Rhizoctonia solani has significantly increased in Heilongjiang Province.Chemical control has become the primary control method.To cope with this,a novel mycelium growth rate method was employed to assess the toxicity of 13 fungicides,including a combination of 45%prochloraz and 125 g·mL^(-1)epoxiconazole,against R.solani.Additionally,the resistance of 99 R.solani strains to thifluzamide across various regions was also evaluated.The findings indicated that 75%trifloxystrobin-tebuconazole exhibited the most effective inhibitory effect,with an effective inhibitory medium concentration(EC50)value of 0.0101μg·mL^(-1).The EC50 values for 20%prothioconazole,125 g·mL^(-1)epoxiconazole,24%thifluzamide,and 50%hexaconazole were all less than 10μg·mL^(-1),indicating a better inhibitory effect on R.solani.The strongest synergistic effect was noted in the mixture of prochloraz and epoxiconazole at a 1:2 ratio,resulting in an EC50 value of 2.9917μg·mL^(-1),and a co-toxicity coefficient of 213.38.Among the 34 strains from Harbin City,the average EC50 value was 196.9341μg·mL^(-1)indicating the highest susceptiblility to thifluzamide.Conversely,15 strains from Shuangyashan City exhibited an average EC50 value of 364.7323μg·mL^(-1),reflecting the lowest sensitivity to thifluzamide.The sensitivity baseline EC50 value for R.solani was 253.8854μg·mL^(-1),with an overall resistance level between 0.1567 and 3.3292,indicating that the resistance level of R.solani in Heilongjiang Province remained low.Therefore,R.solani was still sensitive to thifluzamide in most areas of Heilongjiang Province,but there was a certain risk of resistance in Qitaihe City,which needed to be continuously monitored.At the same time,this study might provide a theoretical foundation for enhancing the prevention and management of the rice sheath blight.
文摘[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The control efficacy of 16% validamycin·thifluzamide SC against S.rolfsii was determined by spraying agents on the whole plant of D.officinale. The significance of differences was analyzed by Duncan＇s new multiple range test （DMRT）. [Result] The control efficacy of 16% validamycin.thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg was 85.33% at 14 d post spraying, higher than that of control agent 4% validamycin AS sprayed at the dose of 80 mg a.i./kg （78.36%）, and equivalent to that of control agent 240 g/L thifluzamide SC sprayed at the dose of 120 mg a.i./kg （90.60%）. [Conclusion] 16% Validamycin·thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg could effectively control the damage of S.rolfsii in D.officinale, and could be popularized in the production.
基金Supported by Circular of the Ministry of Agriculture on Approval of the First Batch of the National Demonstration Zones for Modern Agriculture(NJF[2010]22)Fundamental Research Funds for the Central Universities(XDJK2015C057,SWU114046)~~
文摘In order to mitigate the occurrence and damage of cowpea anthracnose and reduce the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statistical analysis were conducted to study the control effect of biopesticide chitosan, biochemical compound Prochloraz-Chitosan and chemical pesticides difenoconazole and thifluzamide by field efficacy trials. The results showed that under serious occurrence of anthracnose in autumn greenhouse cowpea, when the 2% chJtosan AS （2 250 g/hm^2）, 46% Prochloraz-Chitosan EW （450 g/hm^2）, 10% difenoconazole WG （900 g/hm^2, CK） or 24% thifluzamide SC （360 ml/hm^2） were applied three times with an interval of 5-8 d, the control efficiency on day 7, 14 and 20 reached 83%, 78% and 73%, respectively. No significant difference was found in control efficiency among the four kinds of agents. These four kinds of agents, especially chitosan and Prochloraz-Chitosan, could be used as ideal agents for controlling anthracnose, as well as for modern agricultural demonstration zones, pollution-free agricultural products, green agricultural products and organic agricultural products,thereby meeting the develop- ment needs of ＇modern agriculture, green agriculture, organic agriculture and ecological agriculture.
