From the whole plant of Thermopsis Licentiana(Leguminoseae) , one new compound, 13-β-hydroxylthermopsine, along with eight known compounds, N-methylcytisine, ther-mopsine, daidzein , trifolirhizin , daidzin , genisti...From the whole plant of Thermopsis Licentiana(Leguminoseae) , one new compound, 13-β-hydroxylthermopsine, along with eight known compounds, N-methylcytisine, ther-mopsine, daidzein , trifolirhizin , daidzin , genistin, daidzein-4',7-diglucoside and genistein-4', 7-diglucoside were isolated and identified on the basis of chemical and spectral data.展开更多
Objective:To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan,Vural&Kckdk against periodoutopathogenie bacteria,its antioxidant activity and cytotoxic effect on various cancer...Objective:To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan,Vural&Kckdk against periodoutopathogenie bacteria,its antioxidant activity and cytotoxic effect on various cancer cell lines.Methods:In vitro antimicrobial activities of elhanol.methanol,ethyl acetate(ElAc,n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria,Aggregatibacter actinnmycelemconilans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion,minimum inhibitory concentration(MIC)and minimal bactericidal concentration(MBC),Antioxidant properties of the extracts were evaluatod by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and p-carotene bleaching methods.Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent.Additionally,cytotoxic activity of the extracts on androgcn-insensilivc prostate cancer,androgen—sensitive prostate cancer,chronic myelogenous leukemia and acute promyelocytic leukemia bunian cancer cell lines were determined by 3-4,5-dimelhylthiazol-2-yh-2,5-diphenyltclrazolium bromide assay.Human gingival fibroblast cells were used as a control.Results:Our data showed that ELAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemitans(MIC:1.562 mg/ml_MHC:3.124 mg/ml.)and Porph yromonas gingiralis(MIC:0.781 mg/mL,MBC:1.562 mg/mL).In antioxidant assays.ElAc extract exhibited also the highest radical scavenging activity[IC_(50)=(30.0±0.3)μg/ml.]and the highest inhibition[(74.35±0.30)%]|against lineloic acide oxidation.The amount of phenolic content of it was also the highest[(l62.5±l.2)μg/mg gallic acid].In cytotoxic assay,only etbanol[IC_(50)=(80.00±1.21)μg/ml.]and EtAc extract[IC_(50)=(70.0±0.9)μg/ml]were toxic on acute promyeloeytic leukemia cells at 20—100μg/mL.P<0.05>.However,no toxic effect was observed on human gingival fibroblast cells.Cunclusions:According to our findings,owing to its antioxidant and cytotoxic potential,EtAc exlrael might include anticancer agents for acute promyelocytic leukemia.展开更多
The Thermopsis genus（Leguminoseae） is comprised of many species, some of which can be used as folk medicine for the treatment of rabies in China. Thermnopsis licentiana is one of the species and has not been studied...The Thermopsis genus（Leguminoseae） is comprised of many species, some of which can be used as folk medicine for the treatment of rabies in China. Thermnopsis licentiana is one of the species and has not been studied yet. The present paper reports the separation and identification of a new quinolizidine alkaloid（crystal A;see Fig. 1）.展开更多
Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces flower pistils with unusual morphological properties. The aim of this study was to determine the factors controlling the inte...Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces flower pistils with unusual morphological properties. The aim of this study was to determine the factors controlling the interesting feature of this plant. Particularly the controlling features of the flower meristem and the pistils that mutations in two genes and helps occurring the number of flower organs and can cause abnormalities, This study has attempted to explore the controlling feature of Thermopsis turcica, the young bud Arabidopsis CLV2, and CRC gene homologs were isolated and analyzed in different tissues. First, with total RNA isolated from the first thread degenerated primers synthesized cDNA expressions, second with a partial length cDNA cloning process made and the replicated set of analyses. The full-length cDNA of genes related to RACE analysis was carried out with the sequencing process. The real-time PCR method was applied for gene functional analyses with buds, sepals, petals and stamen and relative expression analysis of genes in the tissue of pistils. Full-length cDNA sequence for the gene CLV2 2557 bp, the encoded protein 716 amino acids, CRC 901 for full-length cDNA sequence of the gene—bp, the encoded protein 174 identified as amino acids. Alignment and phylogenetic analyses of CLV2 and CRC genes were found to be similar to other types of legumes in homologous sets. Real-time (RT-qPCR) PCR results relative to study courses with genes.展开更多
基金Supported by the National Natural Science Foundation of China the Foundation for the Doctoral Programme of the Educational Commission of China
文摘From the whole plant of Thermopsis Licentiana(Leguminoseae) , one new compound, 13-β-hydroxylthermopsine, along with eight known compounds, N-methylcytisine, ther-mopsine, daidzein , trifolirhizin , daidzin , genistin, daidzein-4',7-diglucoside and genistein-4', 7-diglucoside were isolated and identified on the basis of chemical and spectral data.
基金Supported by Minsitry of Devetopment of Tuckish Republie for Foundation of Moleculer Biology Research Center(Grant No.1998K121480)
文摘Objective:To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan,Vural&Kckdk against periodoutopathogenie bacteria,its antioxidant activity and cytotoxic effect on various cancer cell lines.Methods:In vitro antimicrobial activities of elhanol.methanol,ethyl acetate(ElAc,n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria,Aggregatibacter actinnmycelemconilans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion,minimum inhibitory concentration(MIC)and minimal bactericidal concentration(MBC),Antioxidant properties of the extracts were evaluatod by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and p-carotene bleaching methods.Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent.Additionally,cytotoxic activity of the extracts on androgcn-insensilivc prostate cancer,androgen—sensitive prostate cancer,chronic myelogenous leukemia and acute promyelocytic leukemia bunian cancer cell lines were determined by 3-4,5-dimelhylthiazol-2-yh-2,5-diphenyltclrazolium bromide assay.Human gingival fibroblast cells were used as a control.Results:Our data showed that ELAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemitans(MIC:1.562 mg/ml_MHC:3.124 mg/ml.)and Porph yromonas gingiralis(MIC:0.781 mg/mL,MBC:1.562 mg/mL).In antioxidant assays.ElAc extract exhibited also the highest radical scavenging activity[IC_(50)=(30.0±0.3)μg/ml.]and the highest inhibition[(74.35±0.30)%]|against lineloic acide oxidation.The amount of phenolic content of it was also the highest[(l62.5±l.2)μg/mg gallic acid].In cytotoxic assay,only etbanol[IC_(50)=(80.00±1.21)μg/ml.]and EtAc extract[IC_(50)=(70.0±0.9)μg/ml]were toxic on acute promyeloeytic leukemia cells at 20—100μg/mL.P<0.05>.However,no toxic effect was observed on human gingival fibroblast cells.Cunclusions:According to our findings,owing to its antioxidant and cytotoxic potential,EtAc exlrael might include anticancer agents for acute promyelocytic leukemia.
基金Present address: Qingdao Chemical Industry Company.
文摘The Thermopsis genus（Leguminoseae） is comprised of many species, some of which can be used as folk medicine for the treatment of rabies in China. Thermnopsis licentiana is one of the species and has not been studied yet. The present paper reports the separation and identification of a new quinolizidine alkaloid（crystal A;see Fig. 1）.
文摘Thermopsis turcica is an endemic species of wild legume in Türkiye. The plant produces flower pistils with unusual morphological properties. The aim of this study was to determine the factors controlling the interesting feature of this plant. Particularly the controlling features of the flower meristem and the pistils that mutations in two genes and helps occurring the number of flower organs and can cause abnormalities, This study has attempted to explore the controlling feature of Thermopsis turcica, the young bud Arabidopsis CLV2, and CRC gene homologs were isolated and analyzed in different tissues. First, with total RNA isolated from the first thread degenerated primers synthesized cDNA expressions, second with a partial length cDNA cloning process made and the replicated set of analyses. The full-length cDNA of genes related to RACE analysis was carried out with the sequencing process. The real-time PCR method was applied for gene functional analyses with buds, sepals, petals and stamen and relative expression analysis of genes in the tissue of pistils. Full-length cDNA sequence for the gene CLV2 2557 bp, the encoded protein 716 amino acids, CRC 901 for full-length cDNA sequence of the gene—bp, the encoded protein 174 identified as amino acids. Alignment and phylogenetic analyses of CLV2 and CRC genes were found to be similar to other types of legumes in homologous sets. Real-time (RT-qPCR) PCR results relative to study courses with genes.
