目的观察芪参还五胶囊对冠心病患者心功能的改善及对外周血Th17/Treg平衡的影响。方法选择于医院治疗的110例冠心病患者,2021年1月—2022年6月为病例入组时间,分组方式为随机数字表法,西医组55例,给予西医基础治疗;芪参还五胶囊组55例,...目的观察芪参还五胶囊对冠心病患者心功能的改善及对外周血Th17/Treg平衡的影响。方法选择于医院治疗的110例冠心病患者,2021年1月—2022年6月为病例入组时间,分组方式为随机数字表法,西医组55例,给予西医基础治疗;芪参还五胶囊组55例,在西医基础治疗同时给予患者芪参还五胶囊治疗。治疗前、后检测两组患者Th17/Treg细胞比例、血红素加氧酶1(HO-1)、一氧化氮(NO)、白细胞介素-35(IL-35)、脂联素(APN)、白细胞介素-17A(IL-17A)、血管性血友病因子(vWF)、脂蛋白相关磷脂酶A2(Lp-PLA2)水平,检测患者冠状动脉微循环指标:冠状动脉血流储备(CFR)、血流量(Qmean)、平均循环抵抗指数(IMR),检测患者心肌耗氧量、左室射血分数(LVEF)、连续窦性R-R间期标准差(SDNN)、24 h QT离散度(QTd),给予患者动则喘息、胸闷气短、心胸隐痛、胸痛反复发作、倦怠懒言、面色㿠白评价,比较西医组及芪参还五胶囊组临床疗效。结果芪参还五胶囊组患者Th17/Treg、IL-17A含量较西医组明显降低(P<0.05),芪参还五胶囊组HO-1、IL-35含量较西医组升高(P<0.005);芪参还五胶囊组患者Lp-PLA2、vWF含量较西医组明显降低(P<0.05),芪参还五胶囊组患者NO、APN含量较西医组升高(P<0.05);芪参还五胶囊组CFR、Qmean较西医组明显升高(P<0.05),芪参还五胶囊组IMR较西医组明显降低(P<0.05);芪参还五胶囊组患者心肌耗氧量、QTd较西医组明显降低(P<0.05),芪参还五胶囊组LVEF、SDNN较西医组升高(P<0.05);芪参还五胶囊组患者心悸易汗、动则喘息、胸闷气短、心胸隐痛、胸痛反复发作、倦怠懒言、面色㿠白评分较西医组明显降低(P<0.05),芪参还五胶囊组总有效率为98.18%,西医组总有效率为83.64%,芪参还五胶囊组患者总有效率高于西医组(P<0.05)。结论芪参还五胶囊治疗冠心病患者,可调节机体Th17/Treg平衡,抑制机体炎症,减少血管内皮损伤,改善患者心功能、冠状动脉微循环及心电图指标,提升患者临床疗效。展开更多
目的观察咳喘镇定汤在小儿咳嗽变异性哮喘治疗中的作用及对患儿嗜酸性粒细胞计数(eosinophil count,EOS)、白介素-23(interleukin-23,IL-23)/辅助性T细胞17(helper T cells17,Th17)轴的影响。方法选取2020年8月—2023年2月收治的小儿咳...目的观察咳喘镇定汤在小儿咳嗽变异性哮喘治疗中的作用及对患儿嗜酸性粒细胞计数(eosinophil count,EOS)、白介素-23(interleukin-23,IL-23)/辅助性T细胞17(helper T cells17,Th17)轴的影响。方法选取2020年8月—2023年2月收治的小儿咳嗽变异性哮喘患儿104例,将患儿采用简单随机法分为两组。常规组给予止咳化痰、抗炎、平喘等常规治疗,咳喘镇定汤组在常规组基础上采用咳喘镇定汤辅助治疗。检测组间及组内T淋巴亚群、EOS、巨噬细胞炎症蛋白-1α(macrophage inflammatory protein-1α,MIP-1α)、Clara细胞分泌蛋白16(clara cell secreted protein 16,CC-16)、嗜酸细胞活化趋化因子(Eotaxin)、IL-23/Th17轴、小气道功能水平。评估组间及组内咳嗽症状评分、中医证候评分差异。统计组间疗效和不良反应。结果治疗前T淋巴亚群、CC-16、Eotaxin等差异无统计学意义(P>0.05)。两组治疗后CD_(8)^(+)、EOS、MIP-1α、Eotaxin降低,CC-16、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)升高,咳喘镇定汤组治疗后CD_(8)^(+)、EOS、MIP-1α、Eotaxin低于常规组,CC-16、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)高于常规组(P<0.05)。治疗前比较IL-23/Th17轴差异无统计学意义(P>0.05)。两组治疗后IL-23、Th17、白介素-17(interleukin-17,IL-17)降低,咳喘镇定汤组治疗后IL-23/Th17轴低于常规组(P<0.05)。治疗前相关评分无差异(P>0.05)。两组治疗后咳嗽症状评分、中医证候评分降低,咳喘镇定汤组治疗后咳嗽症状评分、中医症候评分低于常规组(P<0.05)。治疗前小气道功能无差异(P>0.05)。两组治疗后小气道功能升高,咳喘镇定汤组治疗后小气道功能高于常规组(P<0.05)。咳喘镇定汤组治愈13例,显效和有效共35例,总有效率92.31%高于常规组,差异有统计学意义(P<0.05)。结论咳喘镇定汤可通过调控小儿咳嗽变异性哮喘患儿IL-23/Th17轴,改善T淋巴亚群和小气道功能,减轻咳嗽症状,提高疗效。展开更多
The aim of this study is to observe the therapeutic effect of Inonotus Obliquus Polysaccharide(IOP)on chronic nonbacterial prostatitis(CNP)and its effect on the helper T cells(Th17)and regulatory T cells(Treg)immune i...The aim of this study is to observe the therapeutic effect of Inonotus Obliquus Polysaccharide(IOP)on chronic nonbacterial prostatitis(CNP)and its effect on the helper T cells(Th17)and regulatory T cells(Treg)immune imbalance.The CNP rat models established by injecting Xiaozhiling injection were randomly divided into the model group,cernilton(40 mg/kg,i.g.)group and low-dose(35 mg/kg,i.g.),medium-dose(70 mg/kg,i.g.)and high-dose(140 mg/kg,i.g.)groups,with the same volume of saline injected into the same site as the control group.The prostate’s wet weight and body mass served as the basis for calculating the prostate index.The serum level of prostate-specific antigen(PSA)was detected by ELISA and the histopathology of prostate tissue was detected by HE staining.The protein expression of Foxp3,ROR-γt and STAT3 in rat prostatic tissue was determined by Western blot.The levels of Th17 and Treg cells infiltrated into the spleen were measured by flow cytometry.The results showed that treatment with IOP significantly reduced the levels of prostate index and serum PSA,and attenuated the pathological injury of the prostate tissue induced by CNP.With respect to samples induced by CNP alone,IOP treatment repressed the increased mRNA levels of IL-6,IL-17,IL-21,IL-23,ROR-γt and STAT3 in prostate tissue,while increasing the mRNA levels of IL-10,TGF-βand Foxp3 in prostate tissue.Meanwhile,IOP treatment attenuated the upregulation of the protein expression levels of ROR-γt and STAT3 in prostate tissue.Additionally,the protein expression of Foxp3 in prostate tissue was increased in the IOP-treated group.Flow cytometry analysis further demonstrated that IOP treatment regulated the balance between Th17 and Treg cells in the spleen in rat with CNP.Our study is the first to elucidate that IOP has significant therapeutic effects on CNP through regulation of Th17/Treg balance.Collectively,the study provides evidence for the potential of IOP to treat CNP.展开更多
文摘目的观察芪参还五胶囊对冠心病患者心功能的改善及对外周血Th17/Treg平衡的影响。方法选择于医院治疗的110例冠心病患者,2021年1月—2022年6月为病例入组时间,分组方式为随机数字表法,西医组55例,给予西医基础治疗;芪参还五胶囊组55例,在西医基础治疗同时给予患者芪参还五胶囊治疗。治疗前、后检测两组患者Th17/Treg细胞比例、血红素加氧酶1(HO-1)、一氧化氮(NO)、白细胞介素-35(IL-35)、脂联素(APN)、白细胞介素-17A(IL-17A)、血管性血友病因子(vWF)、脂蛋白相关磷脂酶A2(Lp-PLA2)水平,检测患者冠状动脉微循环指标:冠状动脉血流储备(CFR)、血流量(Qmean)、平均循环抵抗指数(IMR),检测患者心肌耗氧量、左室射血分数(LVEF)、连续窦性R-R间期标准差(SDNN)、24 h QT离散度(QTd),给予患者动则喘息、胸闷气短、心胸隐痛、胸痛反复发作、倦怠懒言、面色㿠白评价,比较西医组及芪参还五胶囊组临床疗效。结果芪参还五胶囊组患者Th17/Treg、IL-17A含量较西医组明显降低(P<0.05),芪参还五胶囊组HO-1、IL-35含量较西医组升高(P<0.005);芪参还五胶囊组患者Lp-PLA2、vWF含量较西医组明显降低(P<0.05),芪参还五胶囊组患者NO、APN含量较西医组升高(P<0.05);芪参还五胶囊组CFR、Qmean较西医组明显升高(P<0.05),芪参还五胶囊组IMR较西医组明显降低(P<0.05);芪参还五胶囊组患者心肌耗氧量、QTd较西医组明显降低(P<0.05),芪参还五胶囊组LVEF、SDNN较西医组升高(P<0.05);芪参还五胶囊组患者心悸易汗、动则喘息、胸闷气短、心胸隐痛、胸痛反复发作、倦怠懒言、面色㿠白评分较西医组明显降低(P<0.05),芪参还五胶囊组总有效率为98.18%,西医组总有效率为83.64%,芪参还五胶囊组患者总有效率高于西医组(P<0.05)。结论芪参还五胶囊治疗冠心病患者,可调节机体Th17/Treg平衡,抑制机体炎症,减少血管内皮损伤,改善患者心功能、冠状动脉微循环及心电图指标,提升患者临床疗效。
文摘目的观察咳喘镇定汤在小儿咳嗽变异性哮喘治疗中的作用及对患儿嗜酸性粒细胞计数(eosinophil count,EOS)、白介素-23(interleukin-23,IL-23)/辅助性T细胞17(helper T cells17,Th17)轴的影响。方法选取2020年8月—2023年2月收治的小儿咳嗽变异性哮喘患儿104例,将患儿采用简单随机法分为两组。常规组给予止咳化痰、抗炎、平喘等常规治疗,咳喘镇定汤组在常规组基础上采用咳喘镇定汤辅助治疗。检测组间及组内T淋巴亚群、EOS、巨噬细胞炎症蛋白-1α(macrophage inflammatory protein-1α,MIP-1α)、Clara细胞分泌蛋白16(clara cell secreted protein 16,CC-16)、嗜酸细胞活化趋化因子(Eotaxin)、IL-23/Th17轴、小气道功能水平。评估组间及组内咳嗽症状评分、中医证候评分差异。统计组间疗效和不良反应。结果治疗前T淋巴亚群、CC-16、Eotaxin等差异无统计学意义(P>0.05)。两组治疗后CD_(8)^(+)、EOS、MIP-1α、Eotaxin降低,CC-16、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)升高,咳喘镇定汤组治疗后CD_(8)^(+)、EOS、MIP-1α、Eotaxin低于常规组,CC-16、CD_(4)^(+)、CD_(4)^(+)/CD_(8)^(+)高于常规组(P<0.05)。治疗前比较IL-23/Th17轴差异无统计学意义(P>0.05)。两组治疗后IL-23、Th17、白介素-17(interleukin-17,IL-17)降低,咳喘镇定汤组治疗后IL-23/Th17轴低于常规组(P<0.05)。治疗前相关评分无差异(P>0.05)。两组治疗后咳嗽症状评分、中医证候评分降低,咳喘镇定汤组治疗后咳嗽症状评分、中医症候评分低于常规组(P<0.05)。治疗前小气道功能无差异(P>0.05)。两组治疗后小气道功能升高,咳喘镇定汤组治疗后小气道功能高于常规组(P<0.05)。咳喘镇定汤组治愈13例,显效和有效共35例,总有效率92.31%高于常规组,差异有统计学意义(P<0.05)。结论咳喘镇定汤可通过调控小儿咳嗽变异性哮喘患儿IL-23/Th17轴,改善T淋巴亚群和小气道功能,减轻咳嗽症状,提高疗效。
基金Shanxi Province Traditional Chinese Medicine Administration Research Project(Grant No.2022ZYYC094)Science and technology innovation project of universities in Shanxi Province(Grant No.2022L342)+1 种基金Shanxi Leader Team of Medical Science&Technology Innovations(Grant No.2020TD02)Discipline Construction Project of Chinese Medicine Chemistry(Grant No.2024XKJS-25).
文摘The aim of this study is to observe the therapeutic effect of Inonotus Obliquus Polysaccharide(IOP)on chronic nonbacterial prostatitis(CNP)and its effect on the helper T cells(Th17)and regulatory T cells(Treg)immune imbalance.The CNP rat models established by injecting Xiaozhiling injection were randomly divided into the model group,cernilton(40 mg/kg,i.g.)group and low-dose(35 mg/kg,i.g.),medium-dose(70 mg/kg,i.g.)and high-dose(140 mg/kg,i.g.)groups,with the same volume of saline injected into the same site as the control group.The prostate’s wet weight and body mass served as the basis for calculating the prostate index.The serum level of prostate-specific antigen(PSA)was detected by ELISA and the histopathology of prostate tissue was detected by HE staining.The protein expression of Foxp3,ROR-γt and STAT3 in rat prostatic tissue was determined by Western blot.The levels of Th17 and Treg cells infiltrated into the spleen were measured by flow cytometry.The results showed that treatment with IOP significantly reduced the levels of prostate index and serum PSA,and attenuated the pathological injury of the prostate tissue induced by CNP.With respect to samples induced by CNP alone,IOP treatment repressed the increased mRNA levels of IL-6,IL-17,IL-21,IL-23,ROR-γt and STAT3 in prostate tissue,while increasing the mRNA levels of IL-10,TGF-βand Foxp3 in prostate tissue.Meanwhile,IOP treatment attenuated the upregulation of the protein expression levels of ROR-γt and STAT3 in prostate tissue.Additionally,the protein expression of Foxp3 in prostate tissue was increased in the IOP-treated group.Flow cytometry analysis further demonstrated that IOP treatment regulated the balance between Th17 and Treg cells in the spleen in rat with CNP.Our study is the first to elucidate that IOP has significant therapeutic effects on CNP through regulation of Th17/Treg balance.Collectively,the study provides evidence for the potential of IOP to treat CNP.
