目的:采用Meta分析的方法评价组织因子途径抑制剂2(TFPI2)基因甲基化在结直肠癌(CRC)和结直肠腺瘤中的筛查诊断价值。方法:检索Pubmed、Cochrane Library、EMBASE、Web of Science、Wanfang、CNKI、VIP、CBM等数据库中相关研究,研究发...目的:采用Meta分析的方法评价组织因子途径抑制剂2(TFPI2)基因甲基化在结直肠癌(CRC)和结直肠腺瘤中的筛查诊断价值。方法:检索Pubmed、Cochrane Library、EMBASE、Web of Science、Wanfang、CNKI、VIP、CBM等数据库中相关研究,研究发表截止时间为2021年2月5日,利用RevMan 5.2、MetaDisc 1.4、Stata12.0等软件进行合并效应量及异质性的统计分析,评估TFPI2基因甲基化的诊断效能。结果:纳入文献11篇,包括788例CRC、422例腺瘤患者和360例正常人。TFPI2甲基化诊断CRC的合并灵敏度为0.82(95%CI:0.67~0.92),合并特异度0.96(95%CI:0.86~0.99),曲线下面积(AUC)为0.96(95%CI:0.94~0.97);诊断结直肠腺瘤的合并灵敏度为0.62(95%CI:0.42~0.79),合并特异度0.94(95%CI:0.83~0.98),AUC为0.89(95%CI:0.86~0.92)。样本类型、种族及文章发表年限可能是TFPI2甲基化诊断CRC异质性的主要来源(P<0.05)。结论:TFPI2基因甲基化对CRC具有较高的诊断价值,在结直肠腺瘤的诊断中也有一定潜力,可用于CRC的早期筛查,值得深入研究。展开更多
<正>Tissue factor pathway inhibitor 2(TFPI2)plays a key role in female reproduction.However,its expression and function in chickens are still unclear.In this study,RNA-seq was performed on ovarian tissues from c...<正>Tissue factor pathway inhibitor 2(TFPI2)plays a key role in female reproduction.However,its expression and function in chickens are still unclear.In this study,RNA-seq was performed on ovarian tissues from chickens aged 30 and 15 weeks to identify the differentially expressed gene TFPI2.The full-length cDNA of TFPI2 was obtained from adult chicken ovaries by rapid-amplification of cDNA ends(RACE),and the putative TFPI2 protein was found to share a highly conserved amino acid sequence with known bird homologs.In addition,TFPI2 was widely expressed in the tissues of adult chicken follicles according to quantitative real-time PCR(qRT-PCR)and Western blotting.Immunohistochemistry suggested that the TFPI2 protein existed in chicken ovary follicles at different developmental states,such as primordial follicles,the ovarian stroma,and the granulosa and theca layers of prehierarchical follicles(6-8 mm)and preovulatory follicles(F1).In vitro,follicle stimulating hormone or luteinizing hormone(FSH/LH)stimulated the expression of TFPI2 in chicken granulosa cells.FSH-/LHinduced TFPI2 mRNA expression was mediated by signaling pathways such as the PKA,PKC,PI3K,and mTOR pathways.Functionally,TFPI2 promoted the proliferation and viability of cultured granulosa cells and decreased the secretion of Progesterone(P4)and Estrogen(E2)and the mRNA abundance of key steroidogenic enzymes(STAR,Cyp17a1,Cyp19a1 and 3B-HSD)as well as MMPs(MMP2,7,9and11).Mechanistically,TFPI2 inhibited the expression of MMP7 via the Wnt signaling pathway.These findings indicate that TFPI2 may play an important role in regulating chicken follicular development and ovulation and suggest the molecular regulation mechanisms.展开更多
基金grants from the Program for Innovative Research Team(in Science and Technology)in University of Henan Province,China(21IRTSTHN022)Zhongyuan Science and the Technology Innovation Leading Scientist Project,China(214200510003)。
文摘<正>Tissue factor pathway inhibitor 2(TFPI2)plays a key role in female reproduction.However,its expression and function in chickens are still unclear.In this study,RNA-seq was performed on ovarian tissues from chickens aged 30 and 15 weeks to identify the differentially expressed gene TFPI2.The full-length cDNA of TFPI2 was obtained from adult chicken ovaries by rapid-amplification of cDNA ends(RACE),and the putative TFPI2 protein was found to share a highly conserved amino acid sequence with known bird homologs.In addition,TFPI2 was widely expressed in the tissues of adult chicken follicles according to quantitative real-time PCR(qRT-PCR)and Western blotting.Immunohistochemistry suggested that the TFPI2 protein existed in chicken ovary follicles at different developmental states,such as primordial follicles,the ovarian stroma,and the granulosa and theca layers of prehierarchical follicles(6-8 mm)and preovulatory follicles(F1).In vitro,follicle stimulating hormone or luteinizing hormone(FSH/LH)stimulated the expression of TFPI2 in chicken granulosa cells.FSH-/LHinduced TFPI2 mRNA expression was mediated by signaling pathways such as the PKA,PKC,PI3K,and mTOR pathways.Functionally,TFPI2 promoted the proliferation and viability of cultured granulosa cells and decreased the secretion of Progesterone(P4)and Estrogen(E2)and the mRNA abundance of key steroidogenic enzymes(STAR,Cyp17a1,Cyp19a1 and 3B-HSD)as well as MMPs(MMP2,7,9and11).Mechanistically,TFPI2 inhibited the expression of MMP7 via the Wnt signaling pathway.These findings indicate that TFPI2 may play an important role in regulating chicken follicular development and ovulation and suggest the molecular regulation mechanisms.
