The Yangtze finless porpoise(Neophocaena asiaeorientalis asiaeorientalis,Pilleri&Gihr,1972;YFP)is an exclusively freshwater cetacean species inhabiting the Yangtze River and its connecting large lakes.As the prima...The Yangtze finless porpoise(Neophocaena asiaeorientalis asiaeorientalis,Pilleri&Gihr,1972;YFP)is an exclusively freshwater cetacean species inhabiting the Yangtze River and its connecting large lakes.As the primary line of defense in maintaining physiological equilibrium,the epidermis of the porpoise is expected to have undergone structural adaptations due to the shift from the marine to the freshwater environment.This study compared the microstructural and ultrastructural features of the epidermis of YFP and its marine counterpart,the East Asian finless porpoise(Neophocaena asiaeorientalis sunameri,Pilleri&Gihr,1975;EAFP).Microscopic structural observations and statistical analyses of the epidermal thickness demonstrated no significant differences in the cell structure or distribution between the two porpoise species.However,the epidermis of the YFP contained more abundant stratum basale cells.The outermost lipid stratum corneum exhibited a thinner cell layer with wider neutral lipid droplets to resist the passive entry of water molecules in the hypotonic environment.In contrast,for the EAFp,a more uniformly arranged stratum basale in the epidermis led to denser keratin fibers and robust desmosomes within each epidermal layer at the ultrastructural level.This tight arrangement of cells can reduce transepidermal water loss(TEWL)in an environment with higher osmotic pressure.In conclusion,the 2 finless porpoise species appear to employ different epidermal mechanisms to adapt to their distinct osmotic environments.The YFP appears to possess a"lipid waterproofing"epidermal structure,while the EAFP possesses a"thick and compact water-retaining"epidermal structure to copewithpotentialwaterloss.展开更多
准确评估皮肤屏障受损程度对指导皮肤相关的护理和治疗具有重要意义。经皮水分散失(trans epidermal water loss, TEWL)是评估皮肤屏障受损的金标准,但其测量复杂且成本高。皮肤镜图像具有简便、低价、无创、实时等优点。为了研究受损...准确评估皮肤屏障受损程度对指导皮肤相关的护理和治疗具有重要意义。经皮水分散失(trans epidermal water loss, TEWL)是评估皮肤屏障受损的金标准,但其测量复杂且成本高。皮肤镜图像具有简便、低价、无创、实时等优点。为了研究受损皮肤屏障的皮肤镜图像相关特征,首先对皮肤镜图像进行白平衡、灰度化、直方图均衡化及维纳滤波预处理,之后提取图像的统计特征、纹理特征和边缘特征,并与皮肤受损造模小鼠的TEWL检测结果进行相关性分析。结果表明,纹理特征与TEWL值之间相关性较高,特别半径为1的局部二值特征的熵相关性最高,相关系数为0.752。皮肤镜的纹理特征能较好刻画皮肤屏障受损程度,有助于替代TEWL开发皮肤屏障功能评价的新方法。展开更多
目的通过皮肤机械屏障障碍模型鼠来研究参苓白术散(Shen Ling Bai Shu Power,SLBZP)对皮肤屏障功能的影响。方法取健康小鼠50只,随机分为5组,即正常对照组、模型组、参苓白术散高、中、低剂量组。参苓白术散各组从第1 d起,分别给予参苓...目的通过皮肤机械屏障障碍模型鼠来研究参苓白术散(Shen Ling Bai Shu Power,SLBZP)对皮肤屏障功能的影响。方法取健康小鼠50只,随机分为5组,即正常对照组、模型组、参苓白术散高、中、低剂量组。参苓白术散各组从第1 d起,分别给予参苓白术散(40g/kg、20g/kg、10g/kg)灌胃,其余各组灌胃给予蒸馏水,1次/d,连续13d。除正常对照组外,其余各组从第9天起,以胶带法复制皮肤机械屏障障碍模型,连续5天。观察参苓白术散对皮肤机械屏障障碍模型鼠皮肤组织形态学变化,并测定透皮水丢失(transepidermal water loss,TEWL),表皮神经酰胺含量及表皮厚度。结果造模小鼠皮肤出现相应的组织病理学改变,参苓白术散各组的病理改变均有不同程度的改善;参苓白术散组小鼠背部皮损处经表皮水分丢失量和表皮厚度显著低于模型组(P<0.05,P<0.01);参苓白术散高、中剂量组表皮神经酰胺含量明显高于模型组(P<0.05)。结论参苓白术散可促进皮肤屏障的修复,增加角质层中神经酰胺含量是其可能的机制之一。展开更多
基金supported by the National Key R&D Program of China(2021YFD1200304).
文摘The Yangtze finless porpoise(Neophocaena asiaeorientalis asiaeorientalis,Pilleri&Gihr,1972;YFP)is an exclusively freshwater cetacean species inhabiting the Yangtze River and its connecting large lakes.As the primary line of defense in maintaining physiological equilibrium,the epidermis of the porpoise is expected to have undergone structural adaptations due to the shift from the marine to the freshwater environment.This study compared the microstructural and ultrastructural features of the epidermis of YFP and its marine counterpart,the East Asian finless porpoise(Neophocaena asiaeorientalis sunameri,Pilleri&Gihr,1975;EAFP).Microscopic structural observations and statistical analyses of the epidermal thickness demonstrated no significant differences in the cell structure or distribution between the two porpoise species.However,the epidermis of the YFP contained more abundant stratum basale cells.The outermost lipid stratum corneum exhibited a thinner cell layer with wider neutral lipid droplets to resist the passive entry of water molecules in the hypotonic environment.In contrast,for the EAFp,a more uniformly arranged stratum basale in the epidermis led to denser keratin fibers and robust desmosomes within each epidermal layer at the ultrastructural level.This tight arrangement of cells can reduce transepidermal water loss(TEWL)in an environment with higher osmotic pressure.In conclusion,the 2 finless porpoise species appear to employ different epidermal mechanisms to adapt to their distinct osmotic environments.The YFP appears to possess a"lipid waterproofing"epidermal structure,while the EAFP possesses a"thick and compact water-retaining"epidermal structure to copewithpotentialwaterloss.
文摘准确评估皮肤屏障受损程度对指导皮肤相关的护理和治疗具有重要意义。经皮水分散失(trans epidermal water loss, TEWL)是评估皮肤屏障受损的金标准,但其测量复杂且成本高。皮肤镜图像具有简便、低价、无创、实时等优点。为了研究受损皮肤屏障的皮肤镜图像相关特征,首先对皮肤镜图像进行白平衡、灰度化、直方图均衡化及维纳滤波预处理,之后提取图像的统计特征、纹理特征和边缘特征,并与皮肤受损造模小鼠的TEWL检测结果进行相关性分析。结果表明,纹理特征与TEWL值之间相关性较高,特别半径为1的局部二值特征的熵相关性最高,相关系数为0.752。皮肤镜的纹理特征能较好刻画皮肤屏障受损程度,有助于替代TEWL开发皮肤屏障功能评价的新方法。
文摘目的通过皮肤机械屏障障碍模型鼠来研究参苓白术散(Shen Ling Bai Shu Power,SLBZP)对皮肤屏障功能的影响。方法取健康小鼠50只,随机分为5组,即正常对照组、模型组、参苓白术散高、中、低剂量组。参苓白术散各组从第1 d起,分别给予参苓白术散(40g/kg、20g/kg、10g/kg)灌胃,其余各组灌胃给予蒸馏水,1次/d,连续13d。除正常对照组外,其余各组从第9天起,以胶带法复制皮肤机械屏障障碍模型,连续5天。观察参苓白术散对皮肤机械屏障障碍模型鼠皮肤组织形态学变化,并测定透皮水丢失(transepidermal water loss,TEWL),表皮神经酰胺含量及表皮厚度。结果造模小鼠皮肤出现相应的组织病理学改变,参苓白术散各组的病理改变均有不同程度的改善;参苓白术散组小鼠背部皮损处经表皮水分丢失量和表皮厚度显著低于模型组(P<0.05,P<0.01);参苓白术散高、中剂量组表皮神经酰胺含量明显高于模型组(P<0.05)。结论参苓白术散可促进皮肤屏障的修复,增加角质层中神经酰胺含量是其可能的机制之一。
