Background: Detection of extended spectrum beta lactamase producing bacteria is an important issue in the clinical settings. Objective: The purpose of the present study was to validate the Cica Beta Test 1 for detecti...Background: Detection of extended spectrum beta lactamase producing bacteria is an important issue in the clinical settings. Objective: The purpose of the present study was to validate the Cica Beta Test 1 for detection of extended spectrum beta-lactamase (ESBL) producing bacteria. Method: This analytical type of cross-sectional study was carried out in the Department of Microbiology and Immunology at Bangabandhu Sheikh Mujib Medical University (B中央人民政府), Dhaka from January 2006 to December 2006 for a period of one (01) year. All the patients presented with the clinical features of urinary tract infection and surgical as well as burn wound infection at any age with both sexes were selected as study population. All bacteria were isolated and identified by their colony morphology, staining characters, pigment production, motility and other relevant biochemical tests. Phenotypic confirmation of ESBLs producing isolates were done by inhibitor potentiated disc diffusion test according to CLSI recommendation. The Cica Beta Test 1 was performed according to the manufacturer’s instructions. Result: A total number of 288 Gram negative bacteria were isolated. Among these isolates Cica Beta test 1 was positive in 97 strains and phenotypic confirmatory test was positive in 89 strains. The test sensitivity of Cica Beta Test 1 was 100% (95% CI 95.9% to 100.0%). Specificity of the test was 96.0% (95% CI 92.2% to 98.2%). The positive predictive value (PPV) and negative predictive value (NPV) were 92.7% (95% CI 84.5% to 95.7%) and 100.0% (95% CI 98.0% to 100.0%) respectively. The accuracy of the test was 97.2% (95% CI 95.1% to 99.1%). Area under ROC curve = 0.980 (95% CI 0.964 to 0.996);p value 0.0001. Conclusion: In conclusion, Cica Beta Test 1 is very high sensitivity and specificity for the detection of ESBL from Gram negative bacteria.展开更多
目的探析老年非致残性脑卒中患者血清dickkopf相关蛋白1(dickkopf-related protein 1,DKK-1)、富亮氨酸α-2糖蛋白1(leucine-richα-2-glycoprotein-1,LRG1)水平变化与认知损害的关系。方法回顾性选择2023年1月至2024年12月衡水市人民...目的探析老年非致残性脑卒中患者血清dickkopf相关蛋白1(dickkopf-related protein 1,DKK-1)、富亮氨酸α-2糖蛋白1(leucine-richα-2-glycoprotein-1,LRG1)水平变化与认知损害的关系。方法回顾性选择2023年1月至2024年12月衡水市人民医院神经内科收治的老年非致残性脑卒中患者114例,根据蒙特利尔认知评估量表(Montreal cognitive assessment,MoCA)评分分为认知损害组35例(MoCA评分<26分)和认知正常组79例(MoCA评分≥26分)。检测DKK-1、LRG1水平,并分析二者水平变化与老年非致残性脑卒中患者认知损害的关系。结果认知损害组年龄、病灶体积及美国国立卫生研究院卒中量表评分明显高于认知正常组,差异有统计学意义(P<0.01)。认知损害组和认知正常组发病2周DKK-1、LRG1水平明显低于入院时,差异有统计学意义(P<0.05)。认知损害组发病2周DKK-1、LRG1水平明显高于认知正常组,差异有统计学意义(P<0.01)。多因素logistic回归分析显示,发病2周DKK-1(OR=1.049,95%CI:1.024~1.074,P<0.01)、LRG1(OR=1.043,95%CI:1.008~1.079,P<0.05)水平、年龄(OR=1.290,95%CI:1.073~1.552,P<0.01)、病灶体积(OR=1.977,95%CI:1.175~3.327,P<0.05)是老年非致残性脑卒中患者发生认知损害的危险因素。ROC曲线分析显示,老年非致残性脑卒中患者发病2周DKK-1、LRG1水平、年龄、病灶体积评估发生认知损害的曲线下面积分别为0.807、0.720、0.661、0.729(P<0.01)。结论老年非致残性脑卒中患者血清DKK-1、LRG1水平持续较高可能与认知损害风险存在一定的关联,临床可借助这两种指标进行辅助评估。展开更多
To determine whether a given deterministic nonlinear dynamic system is chaotic or periodic, a novel test approach named zero-one （0-1） test has been proposed recently. In this approach, the regular and chaotic motio...To determine whether a given deterministic nonlinear dynamic system is chaotic or periodic, a novel test approach named zero-one （0-1） test has been proposed recently. In this approach, the regular and chaotic motions can be decided by calculating the parameter K approaching asymptotically to zero or one. In this study, we focus on the 0-1 test algorithm and illustrate the selection of parameters of this algorithm by numerical experiments. To validate the reliability and the universality of this algorithm, it is applied to typical nonlinear dynamic systems, including fractional-order dynamic system.展开更多
Experimental data taken from free-field soil in 1-g shaking table tests are valuable for seismic studies on soil-structure interaction.But the available data from medium-to large-scale shaking table tests were not abu...Experimental data taken from free-field soil in 1-g shaking table tests are valuable for seismic studies on soil-structure interaction.But the available data from medium-to large-scale shaking table tests were not abundant enough to cover a large variety of types and conditions of the soil.In the study,1-g shaking table tests of a 3-m-height sand column were conducted to provide seismic experimental data about sand.The sand was directly collected in-situ,with the largest grain diameter being 2 cm and containing a water content of 6.3%.Properties of the sand were estimated under the influence of white noise plus pulse and earthquake motions,including the settlement,the dynamic properties of the sand column,and the three soil layers′shear modulus degradation relationships.The estimated properties were then indirectly verified by means of finite element analysis.Results show that the estimated parameters were effective and could be used in numerical modeling to reproduce approximate seismic responses of the sand column.展开更多
Multiple endocrine neoplasia type 1(MEN1) is an autosomal dominant inherited tumour syndrome expressing various endocrine and non-endocrine lesions and tumours. Since the identification of the causative gene, the onco...Multiple endocrine neoplasia type 1(MEN1) is an autosomal dominant inherited tumour syndrome expressing various endocrine and non-endocrine lesions and tumours. Since the identification of the causative gene, the oncosuppressor gene MEN1, in 1997, genetic testing has revealed an important approach for the early and differential diagnosis of the disease. The finding of a MEN1 mutation in a patient has important clinical implications for relatives since it allows very early disease diagnosis and identification of carriers, even before biochemical and/or clinical manifestation, permitting their inclusion in a specific program of surveillance and subsequent praecox therapy. Currently, genetic testing for MEN1 consists principally of the sequencing of coding regions and intron-exon junctions of the MEN1 gene. However, the recent acquisition of novel high throughput technologies will allow the design of innovative, accurate, complete and rapid genetic diagnosis. These new tools are able to increase the strength of the analysis and almost completely eliminate the possibility of false negative results. This review aims to give an overview on genetic testing of MEN1 syndrome, reporting the positive aspects of performing the analysis and the future perspectives for improving the performance of the test, as well as its application in clinical practice.展开更多
文摘Background: Detection of extended spectrum beta lactamase producing bacteria is an important issue in the clinical settings. Objective: The purpose of the present study was to validate the Cica Beta Test 1 for detection of extended spectrum beta-lactamase (ESBL) producing bacteria. Method: This analytical type of cross-sectional study was carried out in the Department of Microbiology and Immunology at Bangabandhu Sheikh Mujib Medical University (B中央人民政府), Dhaka from January 2006 to December 2006 for a period of one (01) year. All the patients presented with the clinical features of urinary tract infection and surgical as well as burn wound infection at any age with both sexes were selected as study population. All bacteria were isolated and identified by their colony morphology, staining characters, pigment production, motility and other relevant biochemical tests. Phenotypic confirmation of ESBLs producing isolates were done by inhibitor potentiated disc diffusion test according to CLSI recommendation. The Cica Beta Test 1 was performed according to the manufacturer’s instructions. Result: A total number of 288 Gram negative bacteria were isolated. Among these isolates Cica Beta test 1 was positive in 97 strains and phenotypic confirmatory test was positive in 89 strains. The test sensitivity of Cica Beta Test 1 was 100% (95% CI 95.9% to 100.0%). Specificity of the test was 96.0% (95% CI 92.2% to 98.2%). The positive predictive value (PPV) and negative predictive value (NPV) were 92.7% (95% CI 84.5% to 95.7%) and 100.0% (95% CI 98.0% to 100.0%) respectively. The accuracy of the test was 97.2% (95% CI 95.1% to 99.1%). Area under ROC curve = 0.980 (95% CI 0.964 to 0.996);p value 0.0001. Conclusion: In conclusion, Cica Beta Test 1 is very high sensitivity and specificity for the detection of ESBL from Gram negative bacteria.
文摘目的探析老年非致残性脑卒中患者血清dickkopf相关蛋白1(dickkopf-related protein 1,DKK-1)、富亮氨酸α-2糖蛋白1(leucine-richα-2-glycoprotein-1,LRG1)水平变化与认知损害的关系。方法回顾性选择2023年1月至2024年12月衡水市人民医院神经内科收治的老年非致残性脑卒中患者114例,根据蒙特利尔认知评估量表(Montreal cognitive assessment,MoCA)评分分为认知损害组35例(MoCA评分<26分)和认知正常组79例(MoCA评分≥26分)。检测DKK-1、LRG1水平,并分析二者水平变化与老年非致残性脑卒中患者认知损害的关系。结果认知损害组年龄、病灶体积及美国国立卫生研究院卒中量表评分明显高于认知正常组,差异有统计学意义(P<0.01)。认知损害组和认知正常组发病2周DKK-1、LRG1水平明显低于入院时,差异有统计学意义(P<0.05)。认知损害组发病2周DKK-1、LRG1水平明显高于认知正常组,差异有统计学意义(P<0.01)。多因素logistic回归分析显示,发病2周DKK-1(OR=1.049,95%CI:1.024~1.074,P<0.01)、LRG1(OR=1.043,95%CI:1.008~1.079,P<0.05)水平、年龄(OR=1.290,95%CI:1.073~1.552,P<0.01)、病灶体积(OR=1.977,95%CI:1.175~3.327,P<0.05)是老年非致残性脑卒中患者发生认知损害的危险因素。ROC曲线分析显示,老年非致残性脑卒中患者发病2周DKK-1、LRG1水平、年龄、病灶体积评估发生认知损害的曲线下面积分别为0.807、0.720、0.661、0.729(P<0.01)。结论老年非致残性脑卒中患者血清DKK-1、LRG1水平持续较高可能与认知损害风险存在一定的关联,临床可借助这两种指标进行辅助评估。
基金Project supported by the National Natural Science Foundation of of China (Grant No. 60672041)
文摘To determine whether a given deterministic nonlinear dynamic system is chaotic or periodic, a novel test approach named zero-one （0-1） test has been proposed recently. In this approach, the regular and chaotic motions can be decided by calculating the parameter K approaching asymptotically to zero or one. In this study, we focus on the 0-1 test algorithm and illustrate the selection of parameters of this algorithm by numerical experiments. To validate the reliability and the universality of this algorithm, it is applied to typical nonlinear dynamic systems, including fractional-order dynamic system.
基金Supported by:National Natural Science Foundation of China under Grant Nos.52008233 and U1839201the National Key Research and Development Program of China under Grant No.2018YFC1504305the Innovative Research Groups of the National Natural Science Foundation of China under Grant No.51421005。
文摘Experimental data taken from free-field soil in 1-g shaking table tests are valuable for seismic studies on soil-structure interaction.But the available data from medium-to large-scale shaking table tests were not abundant enough to cover a large variety of types and conditions of the soil.In the study,1-g shaking table tests of a 3-m-height sand column were conducted to provide seismic experimental data about sand.The sand was directly collected in-situ,with the largest grain diameter being 2 cm and containing a water content of 6.3%.Properties of the sand were estimated under the influence of white noise plus pulse and earthquake motions,including the settlement,the dynamic properties of the sand column,and the three soil layers′shear modulus degradation relationships.The estimated properties were then indirectly verified by means of finite element analysis.Results show that the estimated parameters were effective and could be used in numerical modeling to reproduce approximate seismic responses of the sand column.
文摘Multiple endocrine neoplasia type 1(MEN1) is an autosomal dominant inherited tumour syndrome expressing various endocrine and non-endocrine lesions and tumours. Since the identification of the causative gene, the oncosuppressor gene MEN1, in 1997, genetic testing has revealed an important approach for the early and differential diagnosis of the disease. The finding of a MEN1 mutation in a patient has important clinical implications for relatives since it allows very early disease diagnosis and identification of carriers, even before biochemical and/or clinical manifestation, permitting their inclusion in a specific program of surveillance and subsequent praecox therapy. Currently, genetic testing for MEN1 consists principally of the sequencing of coding regions and intron-exon junctions of the MEN1 gene. However, the recent acquisition of novel high throughput technologies will allow the design of innovative, accurate, complete and rapid genetic diagnosis. These new tools are able to increase the strength of the analysis and almost completely eliminate the possibility of false negative results. This review aims to give an overview on genetic testing of MEN1 syndrome, reporting the positive aspects of performing the analysis and the future perspectives for improving the performance of the test, as well as its application in clinical practice.
