旨在探究线粒体靶向抗氧化剂Mito-TEMPO在鸡精液冷冻保存中的应用效果,为鸡精液解冻后的精子品质提高提供依据。在精液冷冻稀释液中添加不同浓度的Mito-TEMPO(0、20、40、60和80μmol/m L),检测解冻后鸡精子的质量指标、抗氧化性能和线...旨在探究线粒体靶向抗氧化剂Mito-TEMPO在鸡精液冷冻保存中的应用效果,为鸡精液解冻后的精子品质提高提供依据。在精液冷冻稀释液中添加不同浓度的Mito-TEMPO(0、20、40、60和80μmol/m L),检测解冻后鸡精子的质量指标、抗氧化性能和线粒体酶活性。结果:与对照组(0μmol/m L Mito-TEMPO)相比,添加40μmol/m L Mito-TEMPO时,解冻后鸡精子的活性、线粒体活性以及顶体、质膜和DNA完整率均显著提高(P<0.05);线粒体超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)和过氧化氢酶(CAT)活性显著提高(P<0.05);琥珀酸脱氢酶(SDH)、L-乳酸脱氢酶(L-LDH)和谷草转氨酶(GOT)活性及丙二醛(MDA)含量显著降低(P<0.05)。结论:在鸡精液冷冻稀释液中添加40μmol/m L的Mito-TEMPO可显著改善解冻后精子的活率和活力,有效保护精子的质膜、顶体和DNA的完整性,提高精子的抗氧化能力和线粒体活性,并减少线粒体相关酶的活性,显著改善鸡精子解冻后的质量。展开更多
High-performance catalyst is significant for the sustainable hydrogen(H_(2))production by electrocatalytic water splitting.Optimizing porous structure and active groups of substrate can promote the interaction of subs...High-performance catalyst is significant for the sustainable hydrogen(H_(2))production by electrocatalytic water splitting.Optimizing porous structure and active groups of substrate can promote the interaction of substrate and active metal particles,enabling excellent catalytic properties and stability.Herein,the optimization strategy of delignification and 2,2,6,6-tetramethylpyperidine-1-oxyl(TEMPO)oxidization was developed to modify the porous structure and active groups of wood substrate,and Ru doped Co/CO_(2)P(Ru-Co/CO_(2)P)nanoparticles were encapsulated into the optimized wood carbon substrate(Ru-Co/CO_(2)P@TDCW)for the efficient pH-universal hydrogen evolution reaction(HER).The nanopore and carboxyl groups were produced by delignification and TEMPO oxidation,which accelerated the dispersion and deposition of Ru-Co/CO_(2)P nanoparticles.The RuCo alloy and RuCoP nanoparticles were produced with the doping of Ru,and more Ru-Co/CO_(2)P nanoparticles were anchored by the delignified and TEMPO oxidized wood carbon(TDCW).As anticipated,the Ru-Co/CO_(2)P@TDCW catalyst exhibited excellent pH-universal HER activity,and only 16.6,93,and 43 mV of overpotentials were required to deliver the current density of 50 mA cm^(-2)in alkaline,neutral,and acidic electrolytes,outperforming the noble Pt/C/TDCW catalyst significantly.In addition,Ru-Co/CO_(2)P@TDCW catalyst presented excellent stability for more than 600 h working at 100 mA cm^(-2)in alkaline solution(1.0 M KOH).Density function theory(DFT)results revealed that energy barriers for the dissociation of H_(2)O and the formation of H_(2)were decreased by the doping of Ru,and the conductivity and efficiency of electron migration were also enhanced.This work demonstrated a strategy to optimize the structure and properties of wood carbon substrate,providing a promising strategy to synthesize high-efficiency catalyst for H_(2)production.展开更多
文摘旨在探究线粒体靶向抗氧化剂Mito-TEMPO在鸡精液冷冻保存中的应用效果,为鸡精液解冻后的精子品质提高提供依据。在精液冷冻稀释液中添加不同浓度的Mito-TEMPO(0、20、40、60和80μmol/m L),检测解冻后鸡精子的质量指标、抗氧化性能和线粒体酶活性。结果:与对照组(0μmol/m L Mito-TEMPO)相比,添加40μmol/m L Mito-TEMPO时,解冻后鸡精子的活性、线粒体活性以及顶体、质膜和DNA完整率均显著提高(P<0.05);线粒体超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)和过氧化氢酶(CAT)活性显著提高(P<0.05);琥珀酸脱氢酶(SDH)、L-乳酸脱氢酶(L-LDH)和谷草转氨酶(GOT)活性及丙二醛(MDA)含量显著降低(P<0.05)。结论:在鸡精液冷冻稀释液中添加40μmol/m L的Mito-TEMPO可显著改善解冻后精子的活率和活力,有效保护精子的质膜、顶体和DNA的完整性,提高精子的抗氧化能力和线粒体活性,并减少线粒体相关酶的活性,显著改善鸡精子解冻后的质量。
基金financially supported by the National Natural Science Foundation of China(32101452)the Research Foundation of Education Bureau of Hunan Province(22B0283)+2 种基金the Hunan Provincial Natural Science Foundation(2022JJ40865)the Talents Research Funding of Central South University of Forestry and Technology(2021YJ007)the Scientific Innovation Fund for Graduate of Central South University of Forestry and Technology(2024CX02005)。
文摘High-performance catalyst is significant for the sustainable hydrogen(H_(2))production by electrocatalytic water splitting.Optimizing porous structure and active groups of substrate can promote the interaction of substrate and active metal particles,enabling excellent catalytic properties and stability.Herein,the optimization strategy of delignification and 2,2,6,6-tetramethylpyperidine-1-oxyl(TEMPO)oxidization was developed to modify the porous structure and active groups of wood substrate,and Ru doped Co/CO_(2)P(Ru-Co/CO_(2)P)nanoparticles were encapsulated into the optimized wood carbon substrate(Ru-Co/CO_(2)P@TDCW)for the efficient pH-universal hydrogen evolution reaction(HER).The nanopore and carboxyl groups were produced by delignification and TEMPO oxidation,which accelerated the dispersion and deposition of Ru-Co/CO_(2)P nanoparticles.The RuCo alloy and RuCoP nanoparticles were produced with the doping of Ru,and more Ru-Co/CO_(2)P nanoparticles were anchored by the delignified and TEMPO oxidized wood carbon(TDCW).As anticipated,the Ru-Co/CO_(2)P@TDCW catalyst exhibited excellent pH-universal HER activity,and only 16.6,93,and 43 mV of overpotentials were required to deliver the current density of 50 mA cm^(-2)in alkaline,neutral,and acidic electrolytes,outperforming the noble Pt/C/TDCW catalyst significantly.In addition,Ru-Co/CO_(2)P@TDCW catalyst presented excellent stability for more than 600 h working at 100 mA cm^(-2)in alkaline solution(1.0 M KOH).Density function theory(DFT)results revealed that energy barriers for the dissociation of H_(2)O and the formation of H_(2)were decreased by the doping of Ru,and the conductivity and efficiency of electron migration were also enhanced.This work demonstrated a strategy to optimize the structure and properties of wood carbon substrate,providing a promising strategy to synthesize high-efficiency catalyst for H_(2)production.
