Quantitative trait loci （QTL） were detected for 8 internal organ traits, 3 carcass length traits, and teat number trait in 214 pigs in a resource population that included 180 F2 individuals. A total of 39 microsatel...Quantitative trait loci （QTL） were detected for 8 internal organ traits, 3 carcass length traits, and teat number trait in 214 pigs in a resource population that included 180 F2 individuals. A total of 39 microsatellite markers were examined on SSC4, SSC6, SSC7, SSC8, and SSC13. The genetic traits included heart weight （HW）, lung weight （LW）, liver and gallbladder weight （LGW）, spleen weight （SPW）, stomach weight （STW）, small intestine weight （S1W）, large intestine weight （LIW）, kidney weight （KW）, carcass length to the first cervical vertebra （CL1）, carcass length to the first thoracic vertebra （CL2）, rib numbers （RNS）, and teat numbers （TNS）. Results indicated that, 3 highly significant QTL （P≤0.01 at chromosome-wise level） for HW （at 30 cM on SSC6）, RNS （at 115 cM on SSC7）, TNS （at 110 cM on SSC7）, and 6 significant QTL （P≤0.05 at chromosome-wise level） for LW （at 119 cM on SSC13）, LGW （at 94 cM on SSC6）, SPW （at 106 cM on SSC8）, SIW （0 cM on SSC4）, LIW （170 cM on SSC 4）, and TNS （at 95 cM on SSC6） were detected. The phenotypic variances for which these QTL were accounted ranged from 0.04 % to 14.06 %. Most of these QTL had not been previously reported.展开更多
A testing method for N-nitrosamines and N-nitrosatable substances in rubber teats and soothers was modified. N-Nitrosamines are generally analyzed using either a nitrogen chemiluminescence detector (NCD) or a thermal ...A testing method for N-nitrosamines and N-nitrosatable substances in rubber teats and soothers was modified. N-Nitrosamines are generally analyzed using either a nitrogen chemiluminescence detector (NCD) or a thermal energy analyzer (TEA). However, because few testing laboratories are equipped with these devices, it is difficult to conduct these tests. Therefore, an analysis method for N-nitrosamines using the more widespread gas chromatography-mass spectrometry (GC-MS) method was improved. In addition, EN 12868 was used to prepare the test solutions because of its worldwide use and compliance with EU regulations. Using GC-MS, EN 12868 method targeting ten kinds of N-nitrosamines was modified. The determination limits of the method were 1.0 -1.5 μg/kg for N-nitrosamines and 4 -6 μg/kg for N-nitrosatable substances. Quantification was possible at 1/5 or less and 1/15 or less, respectively, of the regulation values listed in EU Directive 93/11/EEC. In terms of application, there were no problems with the selectivity of the detector. The recoveries were 58% -109% for N-nitrosamines and 59% -102% for N-nitrosatable substances. Screening and verification were possible by measuring the amount of secondary amines in the boiled solution and migration solution.展开更多
目的对京津冀各血站实验室核酸混样模式的单阳性率进行分析,对比各实验室混检单阳性率的差异,探讨各实验室在实验过程的差别和影响因素,为推进京津冀血站的同质化建设提供依据。方法根据15家血液中心/中心血站2018京津冀血液筛查实验室...目的对京津冀各血站实验室核酸混样模式的单阳性率进行分析,对比各实验室混检单阳性率的差异,探讨各实验室在实验过程的差别和影响因素,为推进京津冀血站的同质化建设提供依据。方法根据15家血液中心/中心血站2018京津冀血液筛查实验室质量指标数据汇总分析,按不同检测系统、相同试剂不同实验室、进口试剂和国产试剂、不同国产检测系统和不同混样模式划分,对使用混样模式进行核酸检测14家实验室的HBV DNA、HCV RNA和HIV RNA检出阳性率,运用统计学方法进行分析。结果京津冀14家实验室采用混样模式中,核酸检测实验室以试剂R1、R2和R3为主;试剂6种使用模式3个项目(依次HBV DNA、HCV RNA、HIV RNA)检出率(/万)分别为6.201 vs 3.399 vs 4.909 vs 4.212 vs 3.592 vs 4.585、0.161 vs 0.078 vs 0.158 vs 0 vs 0.218 vs 0.955、0.032 vs 0.158 vs 0.158 vs 0.301 vs 0 vs 1.146,差别均具有统计学意义(P<0.05);3种(R1、R2、R4)国产试剂检测系统HBV DNA的检出率(/万)分别为6.201 vs 3.399 vs 4.212,差别具有统计学意义(P<0.05);使用2种试剂组合的2家实验室间,3个项目检出率(/万)分别为3.592 vs 4.585、0.218 vs 0.955、0 vs 1.146,差别均具有统计学意义(P<0.05);使用R1试剂的3家实验室间HBV DNA检出率(/万)分别为7.197 vs 7.590 vs 7.776,差别不具有统计学意义(P>0.05),而HCV RNA和HIV RNA检出率(/万)分别为0 vs 0.281 vs 0.933、0 vs 0.141 vs 0,差别具有统计学意义(P<0.05);使用R2试剂的5家实验室间3个项目的检出率(/万)分别为3.812 vs 3.849 vs 3.745 vs 1.557 vs 1.542、0 vs 0.367 vs 0 vs 0 vs 0、0 vs 0.183 vs 0.250 vs 0.311 vs 0,差别均具有统计学意义(P<0.05);使用R3试剂的实验室间3个项目的检出率,差别均不具有统计学意义(P>0.05);进口试剂R3混样模式为6混,国产试剂均为8混,两者的HBV DNA、HCV RNA和HIV RNA检出率,差别均不具有统计学意义(P>0.05)。结论京津冀14家血站核酸检测实验室以国产试剂混样模式为主,血站核酸混样检测实验室的HBV DNA检出率明显高于HCV RNA和HIV RNA,且不同试剂间、相同试剂不同实验室存在不同程度差异。京津冀各血站需持续评估各自实验室单阳性率的差别,通过单阳性率比对分析,进一步提升血站实验室检测能力,促进血液检测同质化建设。展开更多
基金This work was supported by the State Key Basic Research and Development Plan of China (No. 2006CB102102)the National Natural Science Foundation of China (No. 30500358).
文摘Quantitative trait loci （QTL） were detected for 8 internal organ traits, 3 carcass length traits, and teat number trait in 214 pigs in a resource population that included 180 F2 individuals. A total of 39 microsatellite markers were examined on SSC4, SSC6, SSC7, SSC8, and SSC13. The genetic traits included heart weight （HW）, lung weight （LW）, liver and gallbladder weight （LGW）, spleen weight （SPW）, stomach weight （STW）, small intestine weight （S1W）, large intestine weight （LIW）, kidney weight （KW）, carcass length to the first cervical vertebra （CL1）, carcass length to the first thoracic vertebra （CL2）, rib numbers （RNS）, and teat numbers （TNS）. Results indicated that, 3 highly significant QTL （P≤0.01 at chromosome-wise level） for HW （at 30 cM on SSC6）, RNS （at 115 cM on SSC7）, TNS （at 110 cM on SSC7）, and 6 significant QTL （P≤0.05 at chromosome-wise level） for LW （at 119 cM on SSC13）, LGW （at 94 cM on SSC6）, SPW （at 106 cM on SSC8）, SIW （0 cM on SSC4）, LIW （170 cM on SSC 4）, and TNS （at 95 cM on SSC6） were detected. The phenotypic variances for which these QTL were accounted ranged from 0.04 % to 14.06 %. Most of these QTL had not been previously reported.
文摘A testing method for N-nitrosamines and N-nitrosatable substances in rubber teats and soothers was modified. N-Nitrosamines are generally analyzed using either a nitrogen chemiluminescence detector (NCD) or a thermal energy analyzer (TEA). However, because few testing laboratories are equipped with these devices, it is difficult to conduct these tests. Therefore, an analysis method for N-nitrosamines using the more widespread gas chromatography-mass spectrometry (GC-MS) method was improved. In addition, EN 12868 was used to prepare the test solutions because of its worldwide use and compliance with EU regulations. Using GC-MS, EN 12868 method targeting ten kinds of N-nitrosamines was modified. The determination limits of the method were 1.0 -1.5 μg/kg for N-nitrosamines and 4 -6 μg/kg for N-nitrosatable substances. Quantification was possible at 1/5 or less and 1/15 or less, respectively, of the regulation values listed in EU Directive 93/11/EEC. In terms of application, there were no problems with the selectivity of the detector. The recoveries were 58% -109% for N-nitrosamines and 59% -102% for N-nitrosatable substances. Screening and verification were possible by measuring the amount of secondary amines in the boiled solution and migration solution.
文摘目的对京津冀各血站实验室核酸混样模式的单阳性率进行分析,对比各实验室混检单阳性率的差异,探讨各实验室在实验过程的差别和影响因素,为推进京津冀血站的同质化建设提供依据。方法根据15家血液中心/中心血站2018京津冀血液筛查实验室质量指标数据汇总分析,按不同检测系统、相同试剂不同实验室、进口试剂和国产试剂、不同国产检测系统和不同混样模式划分,对使用混样模式进行核酸检测14家实验室的HBV DNA、HCV RNA和HIV RNA检出阳性率,运用统计学方法进行分析。结果京津冀14家实验室采用混样模式中,核酸检测实验室以试剂R1、R2和R3为主;试剂6种使用模式3个项目(依次HBV DNA、HCV RNA、HIV RNA)检出率(/万)分别为6.201 vs 3.399 vs 4.909 vs 4.212 vs 3.592 vs 4.585、0.161 vs 0.078 vs 0.158 vs 0 vs 0.218 vs 0.955、0.032 vs 0.158 vs 0.158 vs 0.301 vs 0 vs 1.146,差别均具有统计学意义(P<0.05);3种(R1、R2、R4)国产试剂检测系统HBV DNA的检出率(/万)分别为6.201 vs 3.399 vs 4.212,差别具有统计学意义(P<0.05);使用2种试剂组合的2家实验室间,3个项目检出率(/万)分别为3.592 vs 4.585、0.218 vs 0.955、0 vs 1.146,差别均具有统计学意义(P<0.05);使用R1试剂的3家实验室间HBV DNA检出率(/万)分别为7.197 vs 7.590 vs 7.776,差别不具有统计学意义(P>0.05),而HCV RNA和HIV RNA检出率(/万)分别为0 vs 0.281 vs 0.933、0 vs 0.141 vs 0,差别具有统计学意义(P<0.05);使用R2试剂的5家实验室间3个项目的检出率(/万)分别为3.812 vs 3.849 vs 3.745 vs 1.557 vs 1.542、0 vs 0.367 vs 0 vs 0 vs 0、0 vs 0.183 vs 0.250 vs 0.311 vs 0,差别均具有统计学意义(P<0.05);使用R3试剂的实验室间3个项目的检出率,差别均不具有统计学意义(P>0.05);进口试剂R3混样模式为6混,国产试剂均为8混,两者的HBV DNA、HCV RNA和HIV RNA检出率,差别均不具有统计学意义(P>0.05)。结论京津冀14家血站核酸检测实验室以国产试剂混样模式为主,血站核酸混样检测实验室的HBV DNA检出率明显高于HCV RNA和HIV RNA,且不同试剂间、相同试剂不同实验室存在不同程度差异。京津冀各血站需持续评估各自实验室单阳性率的差别,通过单阳性率比对分析,进一步提升血站实验室检测能力,促进血液检测同质化建设。
