Cartilage injuries remain a significant therapeutic challenge due to cartilage's limited capacity for self-repair.Traditional treatment approaches for cartilage injuries,including surgical interventions,microfract...Cartilage injuries remain a significant therapeutic challenge due to cartilage's limited capacity for self-repair.Traditional treatment approaches for cartilage injuries,including surgical interventions,microfracture and autologous chondrocyte implantation,may alleviate symptoms but frequently present limitations such as surgical trauma,uncertain efficacy,and adverse effects.Active ingredients derived from traditional Chinese medicine(TCM),which possess anti-inflammatory,anti-apoptotic,proliferation-promoting,and differentiation-inducing properties,have emerged as a promising research focus for treating cartilage damage.However,clinical applications of TCM have been constrained by factors including low bioavailability,insufficient stability,and limited targeting capabilities.The integration of TCM active ingredients with biomaterials offers novel solutions to these challenges.This article examines the pathological foundations of cartilage injury,the mechanisms through which TCM active ingredients such as polysaccharides,flavonoids,terpenoids,aldehydes,polyphenols,and saponins address cartilage injury,and the therapeutic applications of TCM active ingredients combined with biomaterials.These biomaterial delivery systems encompass gels,nanomaterials,microspheres,and tissue scaffolds.Additionally,this article analyzes the challenges and potential development trajectories for combining TCM active ingredients with biomaterials in cartilage injury treatment,offering new perspectives for therapeutic intervention.展开更多
Objective： Triple-negative breast cancer（TNBC） is highly invasive and metastatic, which is in urgent need of transformative therapeutics. Tubeimu（TBM）, the rhizome of Bolbostemma paniculatum（Maxim.） Franquet, i...Objective： Triple-negative breast cancer（TNBC） is highly invasive and metastatic, which is in urgent need of transformative therapeutics. Tubeimu（TBM）, the rhizome of Bolbostemma paniculatum（Maxim.） Franquet, is one of the Chinese medicinal herbs used for breast diseases since the ancient times. The present study evaluated the efficacy, especially the anti-metastatic effects of the dichloromethane extract of Tubeimu（ETBM） on TNBC orthotopic mouse models and cell lines.Methods： We applied real-time imaging on florescent orthotopic TNBC mice model and tested cell migration and invasion abilities with MDA-MB-231 cell line. Digital gene expression sequencing was performed and Kyoto Encyclopedia of Genes and Genomes（KEGG） analysis applied to explore the pathways influenced by ETBM.Moreover, quantitative real-time polymerase chain reactions（q RT-PCR） and Western blot were delivered to confirm the gene expression changes.Results： ETBM exhibited noticeable control on tumor metastasis and growth of TNBC tumors with no obvious toxicity. In compliance with this, it also showed inhibition of cell migration and invasion in vitro. Its impact on the changed biological behavior in TNBC may be a result of decreased expression of integrin β1（ITGβ1）, integrin β8（ITGβ8） and Rho GTPase activating protein 5（ARHGAP5）, which disabled the focal adhesion pathway and caused change in cell morphology.Conclusions： This study reveals that ETBM has anti-metastatic effects on MDA-MB-231-GFP tumor and may lead to a new therapeutic agent for the integrative treatment of highly invasive TNBC.展开更多
基金supported by Military Pharmacy Key Discipline Specialty Group Construction Project(Nos.2024-HJZDXK-JS-12,2024-HJZDXK-SZ-01)Shanghai Municipal Health Commission Project(No.2024QN057)+4 种基金Logistics Research Projects of PLA(No.BHJ22J020)Key Research and Development Program of China(Nos.2023YFF1105400,2022YFC3502000)National Natural Science Foundation of China(Nos.82430119,82173704)Sailing Talent Program of Naval Medical UniversityTeaching Achievement Cultivation Project of Naval Medical University(No.JPY2024B06)。
文摘Cartilage injuries remain a significant therapeutic challenge due to cartilage's limited capacity for self-repair.Traditional treatment approaches for cartilage injuries,including surgical interventions,microfracture and autologous chondrocyte implantation,may alleviate symptoms but frequently present limitations such as surgical trauma,uncertain efficacy,and adverse effects.Active ingredients derived from traditional Chinese medicine(TCM),which possess anti-inflammatory,anti-apoptotic,proliferation-promoting,and differentiation-inducing properties,have emerged as a promising research focus for treating cartilage damage.However,clinical applications of TCM have been constrained by factors including low bioavailability,insufficient stability,and limited targeting capabilities.The integration of TCM active ingredients with biomaterials offers novel solutions to these challenges.This article examines the pathological foundations of cartilage injury,the mechanisms through which TCM active ingredients such as polysaccharides,flavonoids,terpenoids,aldehydes,polyphenols,and saponins address cartilage injury,and the therapeutic applications of TCM active ingredients combined with biomaterials.These biomaterial delivery systems encompass gels,nanomaterials,microspheres,and tissue scaffolds.Additionally,this article analyzes the challenges and potential development trajectories for combining TCM active ingredients with biomaterials in cartilage injury treatment,offering new perspectives for therapeutic intervention.
基金supported by National Natural Science Foundation of China Grant (No. 81303129)Beijing University of Chinese Medicine Grant (Project ID: 2016-jxs-548)
文摘Objective： Triple-negative breast cancer（TNBC） is highly invasive and metastatic, which is in urgent need of transformative therapeutics. Tubeimu（TBM）, the rhizome of Bolbostemma paniculatum（Maxim.） Franquet, is one of the Chinese medicinal herbs used for breast diseases since the ancient times. The present study evaluated the efficacy, especially the anti-metastatic effects of the dichloromethane extract of Tubeimu（ETBM） on TNBC orthotopic mouse models and cell lines.Methods： We applied real-time imaging on florescent orthotopic TNBC mice model and tested cell migration and invasion abilities with MDA-MB-231 cell line. Digital gene expression sequencing was performed and Kyoto Encyclopedia of Genes and Genomes（KEGG） analysis applied to explore the pathways influenced by ETBM.Moreover, quantitative real-time polymerase chain reactions（q RT-PCR） and Western blot were delivered to confirm the gene expression changes.Results： ETBM exhibited noticeable control on tumor metastasis and growth of TNBC tumors with no obvious toxicity. In compliance with this, it also showed inhibition of cell migration and invasion in vitro. Its impact on the changed biological behavior in TNBC may be a result of decreased expression of integrin β1（ITGβ1）, integrin β8（ITGβ8） and Rho GTPase activating protein 5（ARHGAP5）, which disabled the focal adhesion pathway and caused change in cell morphology.Conclusions： This study reveals that ETBM has anti-metastatic effects on MDA-MB-231-GFP tumor and may lead to a new therapeutic agent for the integrative treatment of highly invasive TNBC.
