运用系统药理学和生物信息学方法,预测并探究国医大师张磊涤浊法经验方治疗肺癌的关键靶点及作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,T...运用系统药理学和生物信息学方法,预测并探究国医大师张磊涤浊法经验方治疗肺癌的关键靶点及作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)数据库筛选涤浊法经验方的有效化合物及作用靶点;在GeneCards数据库获取肺癌相关靶点,与药物作用靶点映射后得到交集作用靶点;利用DAVID数据库进行基因功能(Gene ontology,GO)及京都基因与基因组数据库(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析;借助TCGA数据库进行差异表达分析,并将差异基因与有效化合物进行分子对接。结果:筛选获得涤浊法经验方有效化合物207个、作用靶点162个;肺癌相关疾病靶点23 701个,映射后得到核心作用靶点51个。DAVID数据库富集分析显示,涤浊法经验方可能通过调控凋亡过程、细胞增殖、基因表达等生物学过程,及TNF信号通路、HIF-1信号通路、PI3K-Akt信号通路等途径发挥抗肺癌作用。“中药-化合物-靶点-通路”综合网络分析表明,槲皮素、木犀草素、山柰酚、汉黄芩素及甘草查耳酮A为作用范围较广的关键有效化合物。差异分析结果显示,相较于低表达者,BCL2、COL1A1、TNFRSF1A基因高表达患者的总体生存率更低(P<0.05);且BCL2、COL1A1在正常组织与肺癌组织中的表达差异具有统计学意义(P<0.05);关键靶点BCL2、COL1A1与关键有效化合物的结合活性良好。结论:初步揭示了涤浊法经验方治疗肺癌的分子机制,挖掘出BCL2、COL1A1有望作为治疗肺癌的关键靶点,为后续深入研究提供了理论依据。展开更多
This study summarized traditional Chinese medicine(TCM)master in the capital,HE Sisheng’s experience in acupuncture on single acupoint application.He emphasizes the properties and therapeutic effects of acupuncture p...This study summarized traditional Chinese medicine(TCM)master in the capital,HE Sisheng’s experience in acupuncture on single acupoint application.He emphasizes the properties and therapeutic effects of acupuncture points in clinical practice,adhering to the principles of balancing Yin and Yang and selecting points along meridians.Acupuncture at Neiguan(PC6)acupoint can clear the heart fire and unclog meridians,harmonize the triple energizer,and treat cardiovascular diseases and gastrointestinal disorders.Acupuncture at Renzhong(GV26)acupoint can awaken the mind,open the orifices,and unblock the governor vessel to treat mental diseases,treating mental disorders and acute lumbar sprain.Acupuncture at Cuanzhu(BL2)acupoint can dispel wind and relieve pain,soothe the spleen and stomach and regulate Qi for headache,eye diseases and hiccup.Acupuncture at Yifeng(SJ17)can nourish Qi,tonify Yang,dispel wind,and unblock meridians,and is effective for diseases of the head and facial features.Acupuncture at Yongquan(KI1)acupoint can invigorate Qi and reinforcing Yang,disperses the wind,passes through the channels and collaterals for diseases of facial features.Acupuncture at Dadun(LR1)acupoint can clear away dampness and heat,regulate Qi and harmonize liver to treat diseases of urogenital system and vertigo and epilepsy.展开更多
文摘运用系统药理学和生物信息学方法,预测并探究国医大师张磊涤浊法经验方治疗肺癌的关键靶点及作用机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)数据库筛选涤浊法经验方的有效化合物及作用靶点;在GeneCards数据库获取肺癌相关靶点,与药物作用靶点映射后得到交集作用靶点;利用DAVID数据库进行基因功能(Gene ontology,GO)及京都基因与基因组数据库(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析;借助TCGA数据库进行差异表达分析,并将差异基因与有效化合物进行分子对接。结果:筛选获得涤浊法经验方有效化合物207个、作用靶点162个;肺癌相关疾病靶点23 701个,映射后得到核心作用靶点51个。DAVID数据库富集分析显示,涤浊法经验方可能通过调控凋亡过程、细胞增殖、基因表达等生物学过程,及TNF信号通路、HIF-1信号通路、PI3K-Akt信号通路等途径发挥抗肺癌作用。“中药-化合物-靶点-通路”综合网络分析表明,槲皮素、木犀草素、山柰酚、汉黄芩素及甘草查耳酮A为作用范围较广的关键有效化合物。差异分析结果显示,相较于低表达者,BCL2、COL1A1、TNFRSF1A基因高表达患者的总体生存率更低(P<0.05);且BCL2、COL1A1在正常组织与肺癌组织中的表达差异具有统计学意义(P<0.05);关键靶点BCL2、COL1A1与关键有效化合物的结合活性良好。结论:初步揭示了涤浊法经验方治疗肺癌的分子机制,挖掘出BCL2、COL1A1有望作为治疗肺癌的关键靶点,为后续深入研究提供了理论依据。
基金supported by“New 3+3 Project”Demonstration Research on Inheritance of“HE’s Tube Acupuncture”Technique“Seven Skills and Five Methods”Project of Beijing Administration of Traditional Chinese Medicine(No.2023-ZYSF-09).
文摘This study summarized traditional Chinese medicine(TCM)master in the capital,HE Sisheng’s experience in acupuncture on single acupoint application.He emphasizes the properties and therapeutic effects of acupuncture points in clinical practice,adhering to the principles of balancing Yin and Yang and selecting points along meridians.Acupuncture at Neiguan(PC6)acupoint can clear the heart fire and unclog meridians,harmonize the triple energizer,and treat cardiovascular diseases and gastrointestinal disorders.Acupuncture at Renzhong(GV26)acupoint can awaken the mind,open the orifices,and unblock the governor vessel to treat mental diseases,treating mental disorders and acute lumbar sprain.Acupuncture at Cuanzhu(BL2)acupoint can dispel wind and relieve pain,soothe the spleen and stomach and regulate Qi for headache,eye diseases and hiccup.Acupuncture at Yifeng(SJ17)can nourish Qi,tonify Yang,dispel wind,and unblock meridians,and is effective for diseases of the head and facial features.Acupuncture at Yongquan(KI1)acupoint can invigorate Qi and reinforcing Yang,disperses the wind,passes through the channels and collaterals for diseases of facial features.Acupuncture at Dadun(LR1)acupoint can clear away dampness and heat,regulate Qi and harmonize liver to treat diseases of urogenital system and vertigo and epilepsy.
