Vitiligo is an immune memory skin disease.T-cell factor 1(TCF1)is essential for maintaining the memory Tcell pool.There is an urgent need to investigate the characteristics of peripheral memory T-cell profile and TCF1...Vitiligo is an immune memory skin disease.T-cell factor 1(TCF1)is essential for maintaining the memory Tcell pool.There is an urgent need to investigate the characteristics of peripheral memory T-cell profile and TCF1^(+)T-cell frequencies in patients with vitiligo.In this study,31 patients with active vitiligo(AV),22 with stable vitiligo(SV),and 30 healthy controls(HCs)were included.We measured circulating memory and TCF1^(+)T-cell frequencies using flow cytometry.The Spearman's rank test was used to evaluate the correlation between cell frequencies and disease characteristics.Receiver operating characteristic curves(ROC)were constructed to investigate the discriminative power of the cell subpopulations.Circulating CD4^(+)and CD8^(+)terminally differentiated effector memory T-cell(TeMRA)frequencies were significantly higher in the AV group than in HCs(P<0.05).TCF1^(+)T-cellsubpopulations were widespread increased in patients with vitiligo(P<0.05).After adjusting for potential confounders,CD8^(+)and CD4^(+)central memory(Tcm)cells,and CD8^(+)TeMRA were correlated with disease activity(P<0.05).The combined diagnostic value of the four(naive,effector memory,TcM,and TeMRA)CD8^(+)TCF1^(+)T-cell subsets was relatively high(area under the ROC curve(AUC)=0.804,sensitivity=71.70%,specificity=83.34%),and the CD8^(+)T-cell subsets combination performed well in discriminating disease activity(AUC=0.849,sensitivity=70.97%,specificity=90.91%).We demonstrated an altered circulating memory T-cell profile and increased TCF1^(+)T-cell percentage in patients with vitiligo.T-cell subpopulations had a strong value for vitiligo diagnosis and activity evaluation.This evidence presents a potential new pharmacological target for inhibiting autoimmunity that leads to vitiligo.展开更多
针对末端夹持激光位移传感器的机器人TCF(tool/terminal control frame)标定,提出一种基于平面模扳的标定方法,机器人操作末端执行器使激光位移传感器在不同位形下对平面模板进行测量,再通过非线性最小.乘拟合求解标定参数.为了减小问...针对末端夹持激光位移传感器的机器人TCF(tool/terminal control frame)标定,提出一种基于平面模扳的标定方法,机器人操作末端执行器使激光位移传感器在不同位形下对平面模板进行测量,再通过非线性最小.乘拟合求解标定参数.为了减小问题的奇异性,对标定时应该采取的参数控制策略进行了定性分析.该标定方法只需要一块表面精度较高的平面模板,而无需其它测量仪器,标定过程简单、易操作,且易于实现自动己.仿真和实验结果表明本文提出的标定方法具有较高的精度.展开更多
基金funded by the National Natural Science Foundation of China(Grant Nos.:82222059,82173416,and 81930087).
文摘Vitiligo is an immune memory skin disease.T-cell factor 1(TCF1)is essential for maintaining the memory Tcell pool.There is an urgent need to investigate the characteristics of peripheral memory T-cell profile and TCF1^(+)T-cell frequencies in patients with vitiligo.In this study,31 patients with active vitiligo(AV),22 with stable vitiligo(SV),and 30 healthy controls(HCs)were included.We measured circulating memory and TCF1^(+)T-cell frequencies using flow cytometry.The Spearman's rank test was used to evaluate the correlation between cell frequencies and disease characteristics.Receiver operating characteristic curves(ROC)were constructed to investigate the discriminative power of the cell subpopulations.Circulating CD4^(+)and CD8^(+)terminally differentiated effector memory T-cell(TeMRA)frequencies were significantly higher in the AV group than in HCs(P<0.05).TCF1^(+)T-cellsubpopulations were widespread increased in patients with vitiligo(P<0.05).After adjusting for potential confounders,CD8^(+)and CD4^(+)central memory(Tcm)cells,and CD8^(+)TeMRA were correlated with disease activity(P<0.05).The combined diagnostic value of the four(naive,effector memory,TcM,and TeMRA)CD8^(+)TCF1^(+)T-cell subsets was relatively high(area under the ROC curve(AUC)=0.804,sensitivity=71.70%,specificity=83.34%),and the CD8^(+)T-cell subsets combination performed well in discriminating disease activity(AUC=0.849,sensitivity=70.97%,specificity=90.91%).We demonstrated an altered circulating memory T-cell profile and increased TCF1^(+)T-cell percentage in patients with vitiligo.T-cell subpopulations had a strong value for vitiligo diagnosis and activity evaluation.This evidence presents a potential new pharmacological target for inhibiting autoimmunity that leads to vitiligo.
文摘针对末端夹持激光位移传感器的机器人TCF(tool/terminal control frame)标定,提出一种基于平面模扳的标定方法,机器人操作末端执行器使激光位移传感器在不同位形下对平面模板进行测量,再通过非线性最小.乘拟合求解标定参数.为了减小问题的奇异性,对标定时应该采取的参数控制策略进行了定性分析.该标定方法只需要一块表面精度较高的平面模板,而无需其它测量仪器,标定过程简单、易操作,且易于实现自动己.仿真和实验结果表明本文提出的标定方法具有较高的精度.
