[Objective] This study aimed to analyze the type III effector tccP and tccP2 genes in Escherichia coli O157：H7 from Chinese water-chestnut. [Method] Gene-specific and locus-specific primers were utilized to amplify t...[Objective] This study aimed to analyze the type III effector tccP and tccP2 genes in Escherichia coli O157：H7 from Chinese water-chestnut. [Method] Gene-specific and locus-specific primers were utilized to amplify tccP/tccP2 and their flanking regions for sequence analysis. [Result] E. coli O157：H7 CWN11 harbored intact tccP and tccP2 genes, however, the number of proline-rich repeats in tccP gene was only one that probably resulted in biological incapability, whereas, the tccP2 gene consisted of five and half proline-rich repeats and could encode functional protein. [Conclusion] Here, we reported the first sequence of tccP gene that consisted of only one proline-rich repeat and tccP2 was assumed to play a crucial role in colonization and subsequent signaling cascades.展开更多
【目的】表皮蛋白是昆虫表皮重要的结构物质,大量研究已经证实表皮蛋白基因参与介导昆虫抗药性形成。以赤拟谷盗(Tribolium castaneum)为研究对象,解析表皮蛋白基因TcCP14.6(cuticle protein CP14.6)和TcLCPA3A(larval cuticle protein ...【目的】表皮蛋白是昆虫表皮重要的结构物质,大量研究已经证实表皮蛋白基因参与介导昆虫抗药性形成。以赤拟谷盗(Tribolium castaneum)为研究对象,解析表皮蛋白基因TcCP14.6(cuticle protein CP14.6)和TcLCPA3A(larval cuticle protein A3A)在昆虫磷化氢抗性形成过程中的作用。【方法】采用联合国粮农组织(FAO)推荐的磷化氢熏蒸方法对采自5个省份赤拟谷盗的磷化氢抗性水平进行测定。基于赤拟谷盗基因组数据获得TcCP14.6和TcLCPA3A的cDNA序列,利用在线生物信息学分析软件预测其编码的氨基酸序列、信号肽和保守结构域。分别提取赤拟谷盗不同组织(头、胸、腹的表皮、翅、足、肠道、马氏管和脂肪体)、不同磷化氢抗性水平以及磷化氢胁迫后试虫的总RNA。以TcRPS和TcRPL为内参基因,运用RT-qPCR技术分析TcCP14.6和TcLCPA3A在赤拟谷盗不同组织、不同磷化氢抗性水平和药剂胁迫下的表达模式。最后,通过RNA干扰技术结合生物测定方法分析TcCP14.6和TcLCPA3A与赤拟谷盗磷化氢抗性的关系。【结果】生物测定结果表明,江苏(JS,RR=1.7)和云南(YN,RR=3.0)种群对磷化氢保持敏感,湖南种群(HN,RR=20.2)表现为中等抗性,四川(SC,RR=395.4)和广东(GD,RR=862.7)种群表现为极高抗。序列分析结果表明TcCP14.6和TcLCPA3A蛋白均包含信号肽和保守的几丁质结合域。RT-qPCR结果显示,TcCP14.6和TcLCPA3A均在赤拟谷盗表皮组织中高表达,而在内部器官(脂肪体、肠道、马氏管)中的表达量相对较低。此外,TcCP14.6表达量随磷化氢抗性水平的增加呈上调趋势,而TcLCPA3A表达量则呈下降趋势;赤拟谷盗经磷化氢熏蒸处理6 h,TcCP14.6和TcLCPA3A的表达量分别呈现上调和下调趋势。注射dsRNA分别干扰抗性(GD)和敏感(YN)种群两个基因的表达后再用LC30的磷化氢浓度处理试虫。与对照相比,TcCP14.6基因沉默后赤拟谷盗的死亡率显著升高,而TcLCPA3A基因沉默后试虫死亡率显著下降。【结论】表皮蛋白基因TcCP14.6和TcLCPA3A参与介导赤拟谷盗对磷化氢的抗性。展开更多
By numerically solving the three-dimensional time-dependent Schrödinger equation,we have investigated multiphoton ionization of hydrogen atom in the two-color circularly polarized(TCCP)laser fields consisting of ...By numerically solving the three-dimensional time-dependent Schrödinger equation,we have investigated multiphoton ionization of hydrogen atom in the two-color circularly polarized(TCCP)laser fields consisting of a strong 400 nm and a much weaker 800 nm pulses.Due to the presence of perturbative 800 nm laser pulse,sideband peaks emerge between the above-threshold ionization rings in the photoelectron momentum distributions.Our numerical results show that the sideband peaks exhibit one-lobe structure in the co-rotating TCCP laser fields,while it displays the three-lobe structure in the counter-rotating TCCP laser fields.Moreover,the photoelectron yield of sidebands in the co-rotating TCCP fields is much higher than those of the counter-rotating TCCP fields.These phenomena could be well explained from the perspective of the photon-absorption channels via the selection rules.Interestingly,an obvious phase shift between the sidebands of different orders from the co-rotating and counter-rotating TCCP fields is observed.This shift indicates the helicity-dependent time delay in the one-photon continuum-continuum transition process.展开更多
基金Supported by the National Key Technology Research and Development Program of China (2012BAK08B07)the National Natural Science Foundation of China (31201919)~~
文摘[Objective] This study aimed to analyze the type III effector tccP and tccP2 genes in Escherichia coli O157：H7 from Chinese water-chestnut. [Method] Gene-specific and locus-specific primers were utilized to amplify tccP/tccP2 and their flanking regions for sequence analysis. [Result] E. coli O157：H7 CWN11 harbored intact tccP and tccP2 genes, however, the number of proline-rich repeats in tccP gene was only one that probably resulted in biological incapability, whereas, the tccP2 gene consisted of five and half proline-rich repeats and could encode functional protein. [Conclusion] Here, we reported the first sequence of tccP gene that consisted of only one proline-rich repeat and tccP2 was assumed to play a crucial role in colonization and subsequent signaling cascades.
文摘【目的】表皮蛋白是昆虫表皮重要的结构物质,大量研究已经证实表皮蛋白基因参与介导昆虫抗药性形成。以赤拟谷盗(Tribolium castaneum)为研究对象,解析表皮蛋白基因TcCP14.6(cuticle protein CP14.6)和TcLCPA3A(larval cuticle protein A3A)在昆虫磷化氢抗性形成过程中的作用。【方法】采用联合国粮农组织(FAO)推荐的磷化氢熏蒸方法对采自5个省份赤拟谷盗的磷化氢抗性水平进行测定。基于赤拟谷盗基因组数据获得TcCP14.6和TcLCPA3A的cDNA序列,利用在线生物信息学分析软件预测其编码的氨基酸序列、信号肽和保守结构域。分别提取赤拟谷盗不同组织(头、胸、腹的表皮、翅、足、肠道、马氏管和脂肪体)、不同磷化氢抗性水平以及磷化氢胁迫后试虫的总RNA。以TcRPS和TcRPL为内参基因,运用RT-qPCR技术分析TcCP14.6和TcLCPA3A在赤拟谷盗不同组织、不同磷化氢抗性水平和药剂胁迫下的表达模式。最后,通过RNA干扰技术结合生物测定方法分析TcCP14.6和TcLCPA3A与赤拟谷盗磷化氢抗性的关系。【结果】生物测定结果表明,江苏(JS,RR=1.7)和云南(YN,RR=3.0)种群对磷化氢保持敏感,湖南种群(HN,RR=20.2)表现为中等抗性,四川(SC,RR=395.4)和广东(GD,RR=862.7)种群表现为极高抗。序列分析结果表明TcCP14.6和TcLCPA3A蛋白均包含信号肽和保守的几丁质结合域。RT-qPCR结果显示,TcCP14.6和TcLCPA3A均在赤拟谷盗表皮组织中高表达,而在内部器官(脂肪体、肠道、马氏管)中的表达量相对较低。此外,TcCP14.6表达量随磷化氢抗性水平的增加呈上调趋势,而TcLCPA3A表达量则呈下降趋势;赤拟谷盗经磷化氢熏蒸处理6 h,TcCP14.6和TcLCPA3A的表达量分别呈现上调和下调趋势。注射dsRNA分别干扰抗性(GD)和敏感(YN)种群两个基因的表达后再用LC30的磷化氢浓度处理试虫。与对照相比,TcCP14.6基因沉默后赤拟谷盗的死亡率显著升高,而TcLCPA3A基因沉默后试虫死亡率显著下降。【结论】表皮蛋白基因TcCP14.6和TcLCPA3A参与介导赤拟谷盗对磷化氢的抗性。
基金This work was supported by the National Key Research and Development Program of China(Grants No.2019YFA0308300)the National Natural Science Foundation of China(Grant Nos.11874163 and 12021004).
文摘By numerically solving the three-dimensional time-dependent Schrödinger equation,we have investigated multiphoton ionization of hydrogen atom in the two-color circularly polarized(TCCP)laser fields consisting of a strong 400 nm and a much weaker 800 nm pulses.Due to the presence of perturbative 800 nm laser pulse,sideband peaks emerge between the above-threshold ionization rings in the photoelectron momentum distributions.Our numerical results show that the sideband peaks exhibit one-lobe structure in the co-rotating TCCP laser fields,while it displays the three-lobe structure in the counter-rotating TCCP laser fields.Moreover,the photoelectron yield of sidebands in the co-rotating TCCP fields is much higher than those of the counter-rotating TCCP fields.These phenomena could be well explained from the perspective of the photon-absorption channels via the selection rules.Interestingly,an obvious phase shift between the sidebands of different orders from the co-rotating and counter-rotating TCCP fields is observed.This shift indicates the helicity-dependent time delay in the one-photon continuum-continuum transition process.
