Tetrachlorobisphenol A(TCBPA),a widely used halogenated flame retardant,is frequently detected in environmental compartments and human samples.However,unknown developmental toxicity and mechanisms limit the entire und...Tetrachlorobisphenol A(TCBPA),a widely used halogenated flame retardant,is frequently detected in environmental compartments and human samples.However,unknown developmental toxicity and mechanisms limit the entire understanding of its effects.In this study,zebrafish(Danio rerio)embryos were exposed to various concentrations of TCBPA while a combination of transcriptomics,behavioral and biochemical analyzes as well as metabolomics were applied to decipher its toxic effects and the potential mechanisms.We found that TCBPA could interfere with nervous and cardiovascular development through focal adhesion and extracellular matrix-receptor(ECM-receptor)interaction pathways through transcriptomic analysis.Behavioral and biochemical analysis results indicated abnormal swimming behavior of zebrafish larvae.Morphological observations revealed that TCBPA could cause the loss of head blood vessels.Metabolomic analysis showed that arginine-related metabolic pathways were one of the main pathways leading to TCBPA developmental toxicity.Our study demonstrated that by using omics,TCBPA was shown to have neurological and cardiovascular developmental toxicity and the underlying mechanisms were uncovered and major pathways identified.展开更多
建立了液相色谱-串联质谱法测定纺织品中四氯双酚A(TCBPA)和四溴双酚A(TBBPA)的残留方法。样品采用乙腈/水(50/50)超声提取,提取液用HLB固相萃取小柱净化,以水-乙腈为流动相,选择C18色谱柱(100 mm×2.1 mm, 1.7μm)进行梯度分离。...建立了液相色谱-串联质谱法测定纺织品中四氯双酚A(TCBPA)和四溴双酚A(TBBPA)的残留方法。样品采用乙腈/水(50/50)超声提取,提取液用HLB固相萃取小柱净化,以水-乙腈为流动相,选择C18色谱柱(100 mm×2.1 mm, 1.7μm)进行梯度分离。采用电喷雾负离子电离模式(ESI-)和多反应监测(MRM)扫描模式,同位素内标法定量。结果显示,TCBPA在0.2~20.0μg/L和TBBPA在0.5~40.0μg/L浓度范围内呈良好线性关系,相关系数(r)均大于0.999,检出限(S/N=3)分别为0.03、0.07μg/kg,平均加标回收率分别为97.4%和94.8%,相对标准偏差范围分别为4.1%~6.3%和4.2%~5.7%。所建方法灵敏度高,特异性强,适用于纺织品中TCBPA和TBBPA的测定,可为纺织品中其他双酚化合物残留检测提供技术方案。展开更多
基金supported by the National Natural Science Foundation of China(Nos.22076214 and 42007226)Beijing Municipal Youth Top-Notch Talent Program(No.2018000021223ZK34).
文摘Tetrachlorobisphenol A(TCBPA),a widely used halogenated flame retardant,is frequently detected in environmental compartments and human samples.However,unknown developmental toxicity and mechanisms limit the entire understanding of its effects.In this study,zebrafish(Danio rerio)embryos were exposed to various concentrations of TCBPA while a combination of transcriptomics,behavioral and biochemical analyzes as well as metabolomics were applied to decipher its toxic effects and the potential mechanisms.We found that TCBPA could interfere with nervous and cardiovascular development through focal adhesion and extracellular matrix-receptor(ECM-receptor)interaction pathways through transcriptomic analysis.Behavioral and biochemical analysis results indicated abnormal swimming behavior of zebrafish larvae.Morphological observations revealed that TCBPA could cause the loss of head blood vessels.Metabolomic analysis showed that arginine-related metabolic pathways were one of the main pathways leading to TCBPA developmental toxicity.Our study demonstrated that by using omics,TCBPA was shown to have neurological and cardiovascular developmental toxicity and the underlying mechanisms were uncovered and major pathways identified.
文摘建立了液相色谱-串联质谱法测定纺织品中四氯双酚A(TCBPA)和四溴双酚A(TBBPA)的残留方法。样品采用乙腈/水(50/50)超声提取,提取液用HLB固相萃取小柱净化,以水-乙腈为流动相,选择C18色谱柱(100 mm×2.1 mm, 1.7μm)进行梯度分离。采用电喷雾负离子电离模式(ESI-)和多反应监测(MRM)扫描模式,同位素内标法定量。结果显示,TCBPA在0.2~20.0μg/L和TBBPA在0.5~40.0μg/L浓度范围内呈良好线性关系,相关系数(r)均大于0.999,检出限(S/N=3)分别为0.03、0.07μg/kg,平均加标回收率分别为97.4%和94.8%,相对标准偏差范围分别为4.1%~6.3%和4.2%~5.7%。所建方法灵敏度高,特异性强,适用于纺织品中TCBPA和TBBPA的测定,可为纺织品中其他双酚化合物残留检测提供技术方案。
