目的:研究转录共结合因子T B L1对根尖牙乳头干细胞成骨定向分化能力的影响。方法:利用慢病毒载体的T B L1 sh R N A基因敲除T B L1进行基因缺失功能研究;通过检测A LP活性、茜素红染色、钙离子定量分析、成骨分化相关基因的表达,研究...目的:研究转录共结合因子T B L1对根尖牙乳头干细胞成骨定向分化能力的影响。方法:利用慢病毒载体的T B L1 sh R N A基因敲除T B L1进行基因缺失功能研究;通过检测A LP活性、茜素红染色、钙离子定量分析、成骨分化相关基因的表达,研究根尖牙乳头干细胞体外成骨分化能力。结果:根尖牙乳头干细胞基因敲除T B L1基因后,干细胞A LP活性、体外矿化能力及成骨分化相关基因骨涎蛋白、骨桥蛋白、骨矿化结合素的表达水平降低,与对照组之间差异均有统计学意义;并且成骨分化关键转录因子R unx 2的表达抑制。结论:本研究揭示了T B L1的新功能,表明T B L1基因表达降低,抑制了根尖牙乳头干细胞体外成骨分化能力,提示转录因子T B L1在根尖牙乳头干细胞成骨定向分化中起正向调控作用。展开更多
BACKGROUND Pancreatic ductal adenocarcinoma(PDAC) is one of the deadliest solid tumors. Identification of diagnostic and therapeutic biomarkers for PDAC is urgently needed. Transducin(β)-like 1 X-linked receptor 1(TB...BACKGROUND Pancreatic ductal adenocarcinoma(PDAC) is one of the deadliest solid tumors. Identification of diagnostic and therapeutic biomarkers for PDAC is urgently needed. Transducin(β)-like 1 X-linked receptor 1(TBL1 XR1) has been linked to the progression of various human cancers. Nevertheless, the function and role of TBL1 XR1 in pancreatic cancers are unclear.AIM To elucidate the function and potential mechanism of TBL1 XR1 in the development of PDAC.METHODS Ninety patients with histologically-confirmed PDAC were included in this study. PDAC tumor samples and cell lines were used to determine the expression of TBL1 XR1. CCK-8 assays and colony formation assays were carried out to assess PDAC cell viability. Flow cytometry was performed to measure the changes in the cell cycle and cell apoptosis. Changes in related protein expression were measured by western blot analysis. Animal analysis was conducted to confirm the impact of TBL1 XR1 in vivo.RESULTS Patients with TBL1 XR1-positive tumors had worse overall survival than those with TBL1 XR1-negative tumors. Moreover, we found that TBL1 XR1 strongly promoted PDAC cell proliferation and inhibited PDAC cell apoptosis. Moreover, knockdown of TBL1 XR1 induced G0/G1 phase arrest. In vivo animal studies confirmed that TBL1 XR1 accelerated tumor cell growth. The results of western blot analysis showed that TBL1 XR1 might play a key role in regulating PDAC cell proliferation and apoptosis via the PI3 K/AKT pathway.CONCLUSION TBL1 XR1 promoted PDAC cell progression and might be an effective diagnostic and therapeutic marker for pancreatic cancer.展开更多
文摘目的:研究转录共结合因子T B L1对根尖牙乳头干细胞成骨定向分化能力的影响。方法:利用慢病毒载体的T B L1 sh R N A基因敲除T B L1进行基因缺失功能研究;通过检测A LP活性、茜素红染色、钙离子定量分析、成骨分化相关基因的表达,研究根尖牙乳头干细胞体外成骨分化能力。结果:根尖牙乳头干细胞基因敲除T B L1基因后,干细胞A LP活性、体外矿化能力及成骨分化相关基因骨涎蛋白、骨桥蛋白、骨矿化结合素的表达水平降低,与对照组之间差异均有统计学意义;并且成骨分化关键转录因子R unx 2的表达抑制。结论:本研究揭示了T B L1的新功能,表明T B L1基因表达降低,抑制了根尖牙乳头干细胞体外成骨分化能力,提示转录因子T B L1在根尖牙乳头干细胞成骨定向分化中起正向调控作用。
文摘BACKGROUND Pancreatic ductal adenocarcinoma(PDAC) is one of the deadliest solid tumors. Identification of diagnostic and therapeutic biomarkers for PDAC is urgently needed. Transducin(β)-like 1 X-linked receptor 1(TBL1 XR1) has been linked to the progression of various human cancers. Nevertheless, the function and role of TBL1 XR1 in pancreatic cancers are unclear.AIM To elucidate the function and potential mechanism of TBL1 XR1 in the development of PDAC.METHODS Ninety patients with histologically-confirmed PDAC were included in this study. PDAC tumor samples and cell lines were used to determine the expression of TBL1 XR1. CCK-8 assays and colony formation assays were carried out to assess PDAC cell viability. Flow cytometry was performed to measure the changes in the cell cycle and cell apoptosis. Changes in related protein expression were measured by western blot analysis. Animal analysis was conducted to confirm the impact of TBL1 XR1 in vivo.RESULTS Patients with TBL1 XR1-positive tumors had worse overall survival than those with TBL1 XR1-negative tumors. Moreover, we found that TBL1 XR1 strongly promoted PDAC cell proliferation and inhibited PDAC cell apoptosis. Moreover, knockdown of TBL1 XR1 induced G0/G1 phase arrest. In vivo animal studies confirmed that TBL1 XR1 accelerated tumor cell growth. The results of western blot analysis showed that TBL1 XR1 might play a key role in regulating PDAC cell proliferation and apoptosis via the PI3 K/AKT pathway.CONCLUSION TBL1 XR1 promoted PDAC cell progression and might be an effective diagnostic and therapeutic marker for pancreatic cancer.
文摘目的:通过将以小组合作,团队共同实践学习为基础的(team-based learning, TBL)教学法引入“智慧树+钉钉课堂”中,探讨该教学法在线上诊断学体格检查授课中的教学效果。方法:随机纳入宁夏医科大学180名临床医学本科生,对照组90人采用传统钉钉线上播放体格检查视频+讲授查体要点+自行练习的教学法,试验组90人采用“智慧树+钉钉课堂”联合TBL教学法。对比两组体格检查技能考核成绩及问卷调查满意度。结果:试验组学生体格检查成绩显著高于对照组(86.68±4.42 vs 78.79±5.96,P<0.001),且在激发学习积极性、提高学习兴趣等多方面满意度更高。结论:该创新教学法可弥补传统线上教学法的不足,有助于提高学生参与临床实践线上课程的兴趣,以及动手实践能力,进而提高临床实践技能考核成绩,改善诊断学体格检查线上授课的教学质量。
