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基于炎症反应探讨核因子κB激酶抑制剂ε/TANK结合激酶1通路在2型糖尿病作用机制的研究进展 被引量:3
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作者 杨玉娇 吕树泉 《中国糖尿病杂志》 北大核心 2025年第1期77-80,共4页
核因子κB(NF-κB)信号通路激活与慢性炎症产生密切相关。多种促炎因子激活NF-κB激酶抑制剂ε/TANK结合激酶1(IKKε/TBK1),胰岛β细胞功能低下或丧失,导致T2DM。抑制或阻断IKKε/TBK1可减少炎性因子产生,降低IR,提高IS,减少T2DM发生。... 核因子κB(NF-κB)信号通路激活与慢性炎症产生密切相关。多种促炎因子激活NF-κB激酶抑制剂ε/TANK结合激酶1(IKKε/TBK1),胰岛β细胞功能低下或丧失,导致T2DM。抑制或阻断IKKε/TBK1可减少炎性因子产生,降低IR,提高IS,减少T2DM发生。本文综述基于炎症反应探讨IKKε/TBK1通路在T2DM作用机制的研究进展。 展开更多
关键词 糖尿病 2型 核因子κB激酶抑制剂ε tank结合激酶1 信号通路 作用机制
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基于改进TANK模型的降雨型滑坡地下水位预测
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作者 郑榆枫 黄栋 +3 位作者 樊晓一 石莉莉 张宇 郭德庆 《人民长江》 北大核心 2025年第1期132-139,共8页
地下水位变化是导致崩塌、滑坡、泥石流等地质灾害发生的重要因素之一。为了更准确地预测降雨型滑坡地下水位的演化情况,考虑降雨强度、地形和地质条件等因素对地下水位变化的影响,提出一种改进的TANK模型。该模型首先采用梯形结构替代... 地下水位变化是导致崩塌、滑坡、泥石流等地质灾害发生的重要因素之一。为了更准确地预测降雨型滑坡地下水位的演化情况,考虑降雨强度、地形和地质条件等因素对地下水位变化的影响,提出一种改进的TANK模型。该模型首先采用梯形结构替代传统的矩形结构,将水位截面由线性变化变为非线性变化;其次重构模型降雨汇集方式以提高水位识别敏感度;最后根据监测数据的有效特征计算并反馈精确度。以塔子坪滑坡监测数据为例进行验证,结果表明所提方法具有良好的可靠性和稳定性。对比传统TANK模型和改进TANK模型的模拟结果与实际监测数据可知,改进TANK模型表现更佳,有训练时间短、对中小雨强-地下水位变化识别敏感、变化趋势更接近监测设备传回结果的特点;在短期、中期、长期预测中,预测精度(NSE)提高了6.8%~20.3%,且中期预测精度(NSE)最高,说明所提模型可以有效地模拟地下水位随降雨的变化趋势。研究成果可为预测和评估降雨型滑坡的地下水状态变化提供依据。 展开更多
关键词 地下水位预测 tank模型 塔子坪滑坡 降雨型滑坡
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平板抽屉式与TANK式除泡工艺及设备
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作者 杨涛 陈勇 贾奇凡 《电子工艺技术》 2025年第4期56-59,共4页
除泡机是使用于液晶显示生产中的重要设备,现生产中使用两种类型设备,分别为平板抽屉式除泡机和TANK式(罐式)除泡机。这两种设备都是对产品进行加压加热进行除泡,只是两种设备由于本身结构的不同,对应使用的工艺参数有所不同。对两种设... 除泡机是使用于液晶显示生产中的重要设备,现生产中使用两种类型设备,分别为平板抽屉式除泡机和TANK式(罐式)除泡机。这两种设备都是对产品进行加压加热进行除泡,只是两种设备由于本身结构的不同,对应使用的工艺参数有所不同。对两种设备进行全面对比,明确其优缺点,灵活采用适应生产。 展开更多
关键词 除泡机 平板抽屉式 tank
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Coupled Effects of Incident Waves Forcing and Internal Tank Sloshing on the Dynamics of Twin Floating Bodies
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作者 Jialong Jiao Mengyun Jiang +1 位作者 Hang Xie Yuanming Chen 《Fluid Dynamics & Materials Processing》 2025年第9期2075-2100,共26页
The growing demand for ocean space has generated significant interest in multi-body floating systems,where gap resonance in confined regions plays a critical role in ensuring the safety of offshore operations.This stu... The growing demand for ocean space has generated significant interest in multi-body floating systems,where gap resonance in confined regions plays a critical role in ensuring the safety of offshore operations.This study develops a numerical tank model using the Smoothed Particle Hydrodynamics(SPH)method,implemented through the open-source code DualSPHysics,to investigate hydrodynamic resonance in a twin-floater system and to examine the influence of internal tank sloshing on its hydrodynamic characteristics.The hydrodynamic behavior of the gap flow between a fixed twin-floater system in the numerical tank is validated through systematic comparison with experimental data.Subsequently,the wave-induced motions and forces on a twin-floater system are compared with those on a single floater.Furthermore,the effects of internal tank sloshing on the hydrodynamic response of the twin-floater system are explored.A parametric study is conducted to analyze the influence of incident wave frequency on floater motion and tank sloshing dynamics.The results show that the presence of an internal tank can significantly reduce pitch motion and vertical forces on the floating body,while exerting minimal influence on heave motion and horizontal forces.The findings provide new insights into the hydrodynamic performance of twin-floater systems and their interaction with internal sloshing phenomena. 展开更多
关键词 SPH method twin-floater gap resonance tank sloshing multi-floating bodies
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Sustainable communal septic tank systems in informal settlements:The case of Lebak Siliwangi,Indonesia
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作者 Kudakwashe Ncube 《Chinese Journal of Population,Resources and Environment》 2025年第1期121-131,共11页
This study focused on realizing Sustainable Development Goal(SDG)6 for inclusive clean water and sanitation;in particular Target 6.3,which aims to reduce untreated wastewater by 2030 while promoting circular wastewate... This study focused on realizing Sustainable Development Goal(SDG)6 for inclusive clean water and sanitation;in particular Target 6.3,which aims to reduce untreated wastewater by 2030 while promoting circular wastewater reuse and recycling globally.The main objective was to assess the adequacy and efficiency of communal septic tank systems in informal settlements while helping local planners and authorities in their decision-making regarding Target 6.3.Quantitative and qualitative approaches were employed with secondary data from previous researchers,and primary data were collected from field surveys,observations,and interviews with members of the local community.The research was delimited to two village administrative divisions known as Rukun Warga(Village Administrative Division,RW):RW 7 and RW 8 of Lebak Siliwangi Kampung in Coblong District,Bandung,West Java,Indonesia.The findings were also compared with situations in other informal settlements in Brazil,Bangladesh,and Nairobi.The results indicated the inadequacy of communal septic tanks in informal settlements due to factors such as substandard system design,limited support and communication between authorities and residents,and the gap between septic tank availability and capacity vis-a-vis demand.Other limiting factors included limited land availability and irregular geomorphology,the latter of which affected the siting and operation of septic tanks due a lack of room for upgrades or expansion in response to continuous population growth.These findings illustrate the need to complement communal septic systems with flexible centralized or decentralized systems to achieve Target 6.3 of SDG 6. 展开更多
关键词 Communal septic tank Informal settlement SUSTAINABILITY Sustainable development goals WASTEWATER
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Improvement on the distribution uniformity of hydrodynamics in a stirred tank with an H-like fractal impeller
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作者 Hui Li Pan You +2 位作者 Xiaoyang Chen Huiming Yang Peicheng Luo 《Chinese Journal of Chemical Engineering》 2025年第6期83-94,共12页
Fractal theory provides a new strategy for equipment design.In this work,we propose a novel H-like fractal(HLF)impeller to improve the uniformity of the distribution of hydrodynamics in stirred tanks.The impellers are... Fractal theory provides a new strategy for equipment design.In this work,we propose a novel H-like fractal(HLF)impeller to improve the uniformity of the distribution of hydrodynamics in stirred tanks.The impellers are constructed by replacing two vertical blades or four legs with two or four H-like subblades by fractal iterations,respectively.Flow characteristics including velocity and turbulent kinetic energy(TKE)distributions,vortices,power number,are predicted by large eddy simulation.Compared with Rushton turbine(RT)impeller when H/T=1(or dual RTs when H/T=1.5,triple RTs when H/T=2),the HLF impeller can produce a flow field with more uniform distributions of larger velocities and TKE level.The impeller with more fractal iteration times can further improve the distribution uniformity of hydrodynamics in the case of high H/T.Power analysis shows that this is mainly due to the improved energy utilization efficiency by the fractal structure design. 展开更多
关键词 FRACTAL IMPELLER Mixing Stirred tank Large eddy simulation(LES)
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Recent advances in time-series analysis methods for identifying fluid flow characteristics in stirred tank reactors
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作者 Xiaoyu Tang Facheng Qiu +3 位作者 Peiqiao Liu Yundong Wang Hong Li Zuohua Liu 《Chinese Journal of Chemical Engineering》 2025年第1期310-327,共18页
Leveraging big data signal processing offers a pathway to the development of artificial intelligencedriven equipment.The analysis of fluid flow signals and the characterization of fluid flow behavior are of critical i... Leveraging big data signal processing offers a pathway to the development of artificial intelligencedriven equipment.The analysis of fluid flow signals and the characterization of fluid flow behavior are of critical in two-phase flow studies.Significant research efforts have focused on discerning flow regimes using various signal analysis methods.In this review,recent advances in time series signals analysis algorithms for stirred tank reactors have been summarized,and the detailed methodologies are categorized into the frequency domain methods,time-frequency domain methods,and state space methods.The strengths,limitations,and notable findings of each algorithm are highlighted.Additionally,the interrelationships between these methodologies have also been discussed,as well as the present progress achieved in various applications.Future research directions and challenges are also predicted to provide an overview of current research trends in data mining of time series for analyzing flow regimes and chaotic signals.This review offers a comprehensive summary for extracting and characterizing fluid flow behavior and serves as a theoretical reference for optimizing the characterization of chaotic signals in future research endeavors. 展开更多
关键词 Flow characteristics Time series analysis Flow signal Chaos analysis Stirred tank reactor
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Diffusion characteristics of deep-sea mining sediment plumes in flowing water by large-scale water tank experiments
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作者 Ze-lin LIU Xiang WU +3 位作者 Qiu-hua RAO Wei YI Shi-ping CHEN Hao ZHENG 《Transactions of Nonferrous Metals Society of China》 2025年第8期2747-2761,共15页
The existing deep-sea sediment plume tests are mostly under small-scale static water and rarely under large-scale flowing water conditions.In this study,large-scale tank experiments of flowing water were designed and ... The existing deep-sea sediment plume tests are mostly under small-scale static water and rarely under large-scale flowing water conditions.In this study,large-scale tank experiments of flowing water were designed and conducted to investigate the morphological characteristics and concentration evolution of the sediment plumes under different discharge rates(Q)and initial sediment concentrations(c).Viscosity tests,resuspension tests and free settling tests of the sediment solution with different c values were performed to reveal the settling mechanism of the plume diffusion process.The results show that the plume diffusion morphology variation in flowing water has four stages and the plume concentration evolution has three stages.The larger the Q,the smaller the initial incidence angle at the discharge outlet,the larger the diffusion range,the poorer the stability and the more complicated the diffusion morphology.The larger the c,the larger the settling velocity,the faster the formation of high-concentration accumulation zone,the better the stability and the clearer the diffusion boundary.The research results could provide experimental data for assessing the impact of deep-sea mining on the ocean environment. 展开更多
关键词 deep-sea mining sediment plumes diffusion characteristics flowing water large-scale water tank experiment
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The 7th China-Latin America and Caribbean Think Tank Forum Held in CIIS
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《China International Studies》 2025年第3期F0002-F0002,共1页
On June 20,the 7th China-Latin America and Caribbean Think Tank Forum was held in Beijing.The forum carried the theme“Working Together to Build a China-LAC Community with a Shared Future.”Assistant Foreign Minister ... On June 20,the 7th China-Latin America and Caribbean Think Tank Forum was held in Beijing.The forum carried the theme“Working Together to Build a China-LAC Community with a Shared Future.”Assistant Foreign Minister Miao Deyu attended the opening ceremony and delivered remarks.Leonel Caraballo Maqueira,Vice President of the Cuban Diplomatic Academy,and Martin Charles,Ambassador of Dominica to China and Representative of the Latin American and Caribbean Diplomatic Corps in China,attended the meeting and delivered speeches. 展开更多
关键词 China Latin America Caribbean Community Latin American Caribbean Diplomatic Corps Cuban Diplomatic Academy Dominica Ambassador China LAC Think tank Forum Shared Future Assistant Foreign Minister Miao Deyu
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大型UNITANK工艺污水处理厂提标工程精细化设计
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作者 司马勤 《净水技术》 2025年第10期188-195,共8页
【目的】针对污水处理厂提标改造中普遍存在的生化脱氮效能受限、水力衔接困难及近远期目标协同难等痛点,文章提出切实可行的优化设计方案,为其他类似工程提供参考。【方法】以广东某20万m^(3)/d污水处理厂提标工程为例,通过对现状构筑... 【目的】针对污水处理厂提标改造中普遍存在的生化脱氮效能受限、水力衔接困难及近远期目标协同难等痛点,文章提出切实可行的优化设计方案,为其他类似工程提供参考。【方法】以广东某20万m^(3)/d污水处理厂提标工程为例,通过对现状构筑物、进出水水质的分析,有针对性地采取现状构筑物挖潜、新增集约化深度处理构筑物的方式实现近期目标,并兼顾与远期目标的衔接。【结果】UNITANK生反池工艺脱氮处理能力不足,近期深挖UNITANK生反池脱氮能力,通过投加碳源,必要时通过增加填料提高微生物浓度从而提高脱氮和除碳效率,将除磷转移至新增深度处理构筑物来实现。面临前端生反池水位和后端接触消毒池水位均受限的不利条件,通过精细化的水力计算和巧妙地设计参数,很好地实现了新增构筑物的水流顺畅,稳定运行。通过集约化布置和采用节地型“高效沉淀池+精密过滤”组合,近期用地指标仅为0.038 m2/(m^(3)·d^(-1)),较国标节约85%。在平均处理水量超负荷7.8%工况下,提标改造工程仍能经济、稳定运行,吨水运行成本仅增加0.18元。【结论】污水处理厂提标工程要基于现状条件,通过现状构筑物充分挖潜与新增构筑物强化相结合,经济合理地实现工程目标。 展开更多
关键词 UNItank 工艺 集约化 高效沉淀池 精密过滤 精细化设计
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Hopfield-Tank模型的收敛性证明
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作者 权光日 洪炳熔 《计算机学报》 EI CSCD 北大核心 1998年第S1期138-141,共4页
本文研究了Hopfield-Tank能量函数在Hopfield网络中的收敛性以及优化率方面的问题.虽然离散的Hopfield网络模型与连续的Hopfield网络模型都有严格的收敛性证明,但是HopfieldTank模型一直没有人给出严格的收敛性证明.本文指出连续的H... 本文研究了Hopfield-Tank能量函数在Hopfield网络中的收敛性以及优化率方面的问题.虽然离散的Hopfield网络模型与连续的Hopfield网络模型都有严格的收敛性证明,但是HopfieldTank模型一直没有人给出严格的收敛性证明.本文指出连续的Hopfield网络模型与Hopfield-Tank模型是有区别的,所以需要另外给出Hopfield-Tank模型的收敛性证明.因此本文给出了Hopfield-Tank模型的收敛性证明,这一证明使Hopfield网络的优化计算理论更加完善.文中还讨论了网络参数1/τ对极小点的影响以及合适的取值范围. 展开更多
关键词 HOPFIELD网络 Hopfield-tank能量函数 Hopfield-tank模型 网络参数1/τ 优化计算理论
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基于Tanks 4.0.9d模型的石化储罐VOCs排放定量方法研究 被引量:24
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作者 李靖 王敏燕 +3 位作者 张健 何万清 聂磊 邵霞 《环境科学》 EI CAS CSCD 北大核心 2013年第12期4718-4723,共6页
石化储罐VOCs排放是石化行业重要的VOCs排放源之一.为了掌握石化储罐VOCs的排放情况,研究了基于Tanks4.0.9d模型计算各种类型储罐VOCs排放量的方法,并对卧式固定顶罐、立式固定顶罐、内浮顶罐和外浮顶罐的VOCs排放量进行了实例计算.同时... 石化储罐VOCs排放是石化行业重要的VOCs排放源之一.为了掌握石化储罐VOCs的排放情况,研究了基于Tanks4.0.9d模型计算各种类型储罐VOCs排放量的方法,并对卧式固定顶罐、立式固定顶罐、内浮顶罐和外浮顶罐的VOCs排放量进行了实例计算.同时,探讨了在国内使用Tanks 4.0.9d模型时需要考虑的所在地气象数据、储罐密封情况、储存物质的参数选择及参数单位换算问题.Tanks 4.0.9d模型可以作为一种方便且准确性较高的石化储罐VOCs排放定量方法在国内推广使用. 展开更多
关键词 tanks 4 0 9d 模型 石化储罐 挥发性有机化合物 排放 定量方法
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Experimental and CFD studies of solid-liquid slurry tank stirred with an improved Intermig impeller 被引量:15
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作者 赵洪亮 张子木 +3 位作者 张廷安 刘燕 顾松青 张超 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2014年第8期2650-2659,共10页
The improved Intermig impeller has been used in the seed precipitation tank in China, which could enhance the mixing and suspension of Al(OH)3 particles and the power consumption declined largely. The flow field, soli... The improved Intermig impeller has been used in the seed precipitation tank in China, which could enhance the mixing and suspension of Al(OH)3 particles and the power consumption declined largely. The flow field, solids hold-up, cloud height, just off-bottom speed and power consumptions were investigated in solid-liquid mixing system with this new type of impeller by CFD and water experiment methods. Compared with the standard Intermig impeller, the improved one coupled with specially sloped baffles could promote the fluid circulation, create better solids suspension and consume less power. Besides lower impeller off-bottom clearance is good for solid suspension and distribution. The just-off-bottom speed was also determined by a power number criterion. Meanwhile, the predicted results were in good agreement with the experimental data. 展开更多
关键词 CFD stirred tank solids suspension mixing improved Intermig impeller
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Bioleaching of Pb-Zn-Sn chalcopyrite concentrate in tank bioreactor and microbial community succession analysis 被引量:7
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作者 王军 赵红波 +3 位作者 庄田 覃文庆 朱珊 邱冠周 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第12期3758-3762,共5页
The variation of microbial community structure was investigated for the tank bioleaching process of Pb-Zn-Sn chalcopyrite concentrate in the presence of mixed moderately thermophilic bacteria. The parameters, such as ... The variation of microbial community structure was investigated for the tank bioleaching process of Pb-Zn-Sn chalcopyrite concentrate in the presence of mixed moderately thermophilic bacteria. The parameters, such as pH value, solution potential and concentrations of metal ions, were determined by the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to analyze the succession of microbial community. The results showed that a final copper extraction rate of 85.6% could be obtained after tank bioleaching for 30 d. The Acidithiobacillus caldus was the dominant population with abundance of about 73.80%in the initial stage, then Sulfobacillus thermosulfidooxidans dominated from the 18th day to the end of bioleaching, while the abundance of Leptospirillum ferriphilum changed slightly. A higher solution potential within a certain range and appropriate concentration of ferric ions were essential for this tank bioleaching of chalcopyrite. 展开更多
关键词 CHALCOPYRITE tank bioleaching microbial community PCR-RFLP technique
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Tank模型在模拟滑坡地下水位中的应用 被引量:7
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作者 洪勇 日浦启全 栾茂田 《吉林大学学报(地球科学版)》 EI CAS CSCD 北大核心 2006年第S1期69-74,共6页
季节性的降雨及其所引起的地下水状态的变化是促使日本大型结晶片岩滑坡活动和诱发灾害发生的重要原因。基于对一典型结晶片岩滑坡、降雨和地下水位的长期观测,利用Tank模型建立了一种模拟滑坡地下水位变化的方法。通过对滑体内不同观... 季节性的降雨及其所引起的地下水状态的变化是促使日本大型结晶片岩滑坡活动和诱发灾害发生的重要原因。基于对一典型结晶片岩滑坡、降雨和地下水位的长期观测,利用Tank模型建立了一种模拟滑坡地下水位变化的方法。通过对滑体内不同观测点地下水位实际观测数据与模拟结果的对比分析,证明所采用的模拟方法能够很好地再现地下水位随降雨的变化形态,从而为预测和评价降雨型滑坡的地下水状态变化提供了依据。 展开更多
关键词 结晶片岩 滑坡 tank模型 地下水 水位模拟
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Optimum design of flow distribution in quenching tank for heat treatment of A357 aluminum alloy large complicated thin-wall workpieces by CFD simulation and ANN approach 被引量:6
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作者 杨夏炜 朱景川 +3 位作者 何东 来忠红 农智升 刘勇 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第5期1442-1451,共10页
Based on the computational fluid dynamics (CFD) method, a quenching tank with two agitator systems and two flow-equilibrating devices was selected to simulate flow distribution using Fluent software. A numerical exa... Based on the computational fluid dynamics (CFD) method, a quenching tank with two agitator systems and two flow-equilibrating devices was selected to simulate flow distribution using Fluent software. A numerical example was used to testify the validity of the quenching tank model. In order to take tank parameters (agitation speed, position of directional flow baffle and coordinate position in quench zone) into account, an approach that combines the artificial neural network (ANN) with CFD method was developed to study the flow distribution in the quenching tank. The flow rate of the quenching medium shows a very good agreement between the ANN predicted results and the Fluent simulated data. Methods for the optimal design of the quenching tank can be used as technical support for industrial production. 展开更多
关键词 A357 aluminum alloy computational fluid dynamics quenching tank flow distribution artificial neural network
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鹿儿岛陡倾边坡地区地下水tank模型构模及应用 被引量:5
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作者 赵晓东 蒋宇静 《岩石力学与工程学报》 EI CAS CSCD 北大核心 2008年第12期2523-2527,共5页
季节性的降雨及其所引起的地下水状态变化是促使日本鹿儿岛竜ヶ水地区崩塌、泥石流和滑坡等灾害发生的重要诱发原因。研究地区年降水量达2000mm左右,地下水的影响造成多次崩塌和泥石流的发生,对周围公路和铁路造成极大的危害。鉴于该地... 季节性的降雨及其所引起的地下水状态变化是促使日本鹿儿岛竜ヶ水地区崩塌、泥石流和滑坡等灾害发生的重要诱发原因。研究地区年降水量达2000mm左右,地下水的影响造成多次崩塌和泥石流的发生,对周围公路和铁路造成极大的危害。鉴于该地区灾害发生与其地质和地下水活动有着紧密的联系,根据多年的降水及钻孔水位的观测资料,建立该地区地下水位模拟的tank模型,并对模型参数进行求解和应用对比分析,为监测预警提供了有力的数据支持。 展开更多
关键词 边坡工程 tank模型 地下水 水位模拟 参数率定
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CFD-supported optimization of flow distribution in quench tank for heat treatment of A357 alloy large complicated components 被引量:3
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作者 杨夏炜 朱景川 李文亚 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第10期3399-3409,共11页
The flow distribution in quench tank for heat treatment of A357 alloy large complicated components was simulated using FLUENT computational fluid dynamics(CFD) software.The flow velocity and the uniformity of flow f... The flow distribution in quench tank for heat treatment of A357 alloy large complicated components was simulated using FLUENT computational fluid dynamics(CFD) software.The flow velocity and the uniformity of flow field in two types of quench tanks(with or without agitation system) were calculated.The results show that the flow field in the quench tank without agitation system has not evident regularity.While as for the quench tank with agitation system,the flow fields in different parameters have certain regularity.The agitation tanks have a distinct advantage over the system without agitation.Proper process parameters were also obtained.Finally,the tank model established in this work was testified by an example from publication.This model with high accuracy is able to optimize the tank structures and can be helpful for industrial production and theoretical investigation in the fields of heat treatment of large complicated components. 展开更多
关键词 A357 alloy flow distribution quench tank computational fluid dynamics(CFD) simulation
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靶向抑制TANK结合激酶1表达对舌及下咽鳞癌细胞增殖及凋亡的影响 被引量:1
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作者 王亚平 邱雁君 +4 位作者 李玲香 崔晓波 张莉 胡文良 贺利平 《听力学及言语疾病杂志》 CAS CSCD 北大核心 2019年第6期626-630,共5页
目的分析靶向抑制头颈部鳞癌细胞中TANK结合酶1(TANK binding,kinase 1,TBK1)表达后细胞增殖与凋亡水平改变。方法采用转染TBK1 siRNA靶向抑制人下咽鳞癌细胞FaDu、人舌鳞癌细胞Cal-27中TBK1的表达,用CCK-8、流式细胞术、Western Blot... 目的分析靶向抑制头颈部鳞癌细胞中TANK结合酶1(TANK binding,kinase 1,TBK1)表达后细胞增殖与凋亡水平改变。方法采用转染TBK1 siRNA靶向抑制人下咽鳞癌细胞FaDu、人舌鳞癌细胞Cal-27中TBK1的表达,用CCK-8、流式细胞术、Western Blot等方法检测FaDu及Cal-27中靶向抑制TBK1表达前后细胞的增殖及凋亡水平;克隆形成实验及细胞增殖实验(CCK-8法)检测梯度浓度氨来呫诺处理后FaDu及Cal-27细胞增殖改变。结果Western Blot结果显示,转染后FaDu及Cal-27细胞中TBK1蛋白表达显著抑制;细胞增殖实验结果显示,靶向抑制TBK1蛋白表达后,FaDu及Cal-27的增殖显著抑制;流式细胞术检测显示FaDu及Cal-27细胞凋亡显著增加;克隆形成及细胞增殖实验结果表明FaDu及Cal-27细胞的增殖随着氨来呫诺浓度增加呈现显著下降(P<0.05)。结论靶向抑制TBK1蛋白表达后,舌及下咽鳞癌细胞的增殖能力明显降低,细胞凋亡显著增加;TBK1抑制剂氨来呫诺对于高表达TBK1的舌及下咽鳞状细胞癌的增殖具有抑制作用。 展开更多
关键词 下咽 鳞状细胞癌 tank结合激酶1 氨来呫诺
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中国海油“CGTank~”大型LNG储罐技术体系与工程化应用 被引量:11
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作者 张超 单彤文 +1 位作者 付子航 肖立 《天然气工业》 EI CAS CSCD 北大核心 2015年第9期95-100,共6页
LNG储罐设计、建造工艺复杂,造价高,是能源领域中的尖端技术之一,长期由国外企业垄断。为了实现LNG储罐技术的国产化,作为目前国内最大LNG进口商的中国海洋石油总公司(以下简称中国海油),在借鉴国内外相关单位设计建设经验的基础上,... LNG储罐设计、建造工艺复杂,造价高,是能源领域中的尖端技术之一,长期由国外企业垄断。为了实现LNG储罐技术的国产化,作为目前国内最大LNG进口商的中国海洋石油总公司(以下简称中国海油),在借鉴国内外相关单位设计建设经验的基础上,经过多年科研攻关,成功研发了LNG储罐技术体系“CGTank”:1自主开发了成套计算软件,通过建立流固耦合的三维全息及多点接触模型,完成了对储罐全工况的计算;2引入欧洲规范,结合响应谱分析,创新了地震作用研究及内罐校核研究;3首次提出利用最大剪切破坏理论得到的主应力作为应力强度判别的标准;4形成了完全自主知识产权的大型LNG全容储罐技术包。“CGTank”体系在天津LNG示范工程中完成了首次应用,由中国海油气电集团提供储罐设计核心技术,由海洋石油工程股份有限责任公司EPC总承包,目前项目已经通过了所有指标的测试,顺利运营。LNG储罐自主技术的应用前景广阔,有利于推动LNG产业的全面国产化进程。 展开更多
关键词 中国海油 LNG 接收站 全容储罐 CGtank 工程应用 国产化 天津LNG示范工程
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