Starch biosynthesis is a critical factor in wheat(Triticum aestivum L.)quality and yield.However,the full scope of its regulation is not fully understood.Here we report that Ta DL interacts with TaB3 and TaNF-YB1 to s...Starch biosynthesis is a critical factor in wheat(Triticum aestivum L.)quality and yield.However,the full scope of its regulation is not fully understood.Here we report that Ta DL interacts with TaB3 and TaNF-YB1 to synergistically regulate starch biosynthesis and quality in wheat.Genome-edited tadl mutant lines had smaller and lighter grains with lower total starch and amylose contents compared to wild type(WT).Correspondingly,the transcript levels of starch biosynthesis-related genes,including TaSUS1,TaSUS2,TaAGPL2,TaSBEIIa,TaGBSSII,and TaSWEET2a,were markedly lower at 15d after flowering(DAF)in tadl mutants.Ta DL physically interacted with TaB3 and TaNFYB1 and activated the transcription of TaSUS2 and TaAGPL2 through direct binding to their promoter regions.A null mutant of TaB3 also affected grain filling,with phenotypes similar to those of tadl mutants,whereas overexpression of TaNF-YB1 promoted grain filling.Our study demonstrated that TaDL plays an essential role in starch biosynthesis and identified an elite allele(TaDL-BI)associated with starch content,providing insights into the underlying molecular mechanism of wheat grain filling,which may be useful in breeding of high-yielding wheat and quality improvement.展开更多
基金supported by the National Natural Science Foundation of China(32472171)the Biological Breeding-National Science and Technology Major Project(2023ZD040680303)+1 种基金Key Research and Development Program of China(2022YFD1200803)the Postdoctoral Fel owship Program(Grade C)of China Postdoctoral Science Foundation(GZC20241946,GZC20233050)。
文摘Starch biosynthesis is a critical factor in wheat(Triticum aestivum L.)quality and yield.However,the full scope of its regulation is not fully understood.Here we report that Ta DL interacts with TaB3 and TaNF-YB1 to synergistically regulate starch biosynthesis and quality in wheat.Genome-edited tadl mutant lines had smaller and lighter grains with lower total starch and amylose contents compared to wild type(WT).Correspondingly,the transcript levels of starch biosynthesis-related genes,including TaSUS1,TaSUS2,TaAGPL2,TaSBEIIa,TaGBSSII,and TaSWEET2a,were markedly lower at 15d after flowering(DAF)in tadl mutants.Ta DL physically interacted with TaB3 and TaNFYB1 and activated the transcription of TaSUS2 and TaAGPL2 through direct binding to their promoter regions.A null mutant of TaB3 also affected grain filling,with phenotypes similar to those of tadl mutants,whereas overexpression of TaNF-YB1 promoted grain filling.Our study demonstrated that TaDL plays an essential role in starch biosynthesis and identified an elite allele(TaDL-BI)associated with starch content,providing insights into the underlying molecular mechanism of wheat grain filling,which may be useful in breeding of high-yielding wheat and quality improvement.
