Constipation is a common gastrointestinal condition.Lactiplantibacillus plantarum T34 has been demonstrated efficacy in relieving loperamide-induced constipation in mice model.Oral intervention of L.plantarum T34(1...Constipation is a common gastrointestinal condition.Lactiplantibacillus plantarum T34 has been demonstrated efficacy in relieving loperamide-induced constipation in mice model.Oral intervention of L.plantarum T34(1×108 CFU/mouse/day)resulted in improved defecation and enhanced intestinal morphology.Mechanistic studies revealed that L.plantarum T34 strengthend the intestinal barrier through the upregulation of tight junction protein genes,specifically Claudin-1,as well as mucosal barrier proteins such as Muc2 and Muc3.Additionally,L.plantarum T34 modulated gut homeostasis by influencing the expression of aquaporin genes,levels of the gut neurotransmitter serotonin(5-HT),and oxidative stress markers.Furthermore,L.plantarum T34 effectively restored the composition of the gastrointestinal microbiota,addressing dysbiosis linked to constipation.These findings suggested that L.plantarum T34 may serve as a promising probiotic intervention for managing constipation,offering a safe and effective alternative to traditional treatments.展开更多
In the present paper we have observed the effect of discontinuous gradient two-step Percoll centrifugation on depleting mature T lymphocytes from normal bone marrow. The pre-/post-Percoll percentage of CD34+, and Leu4...In the present paper we have observed the effect of discontinuous gradient two-step Percoll centrifugation on depleting mature T lymphocytes from normal bone marrow. The pre-/post-Percoll percentage of CD34+, and Leu4+ cells in MNC was counted by using APAAP technique. As the two-step Percoll centrifugation is simple,and time saving, and decreases the incidence of contamination of cultured cells as well,This technique may be of value in serum-free culture of hematopoietic cells and immunological studies.展开更多
基金funded by the Project of Zhejiang Provincial Department of Agriculture and Rural Affairs,Grant Number:2024SNJF055.
文摘Constipation is a common gastrointestinal condition.Lactiplantibacillus plantarum T34 has been demonstrated efficacy in relieving loperamide-induced constipation in mice model.Oral intervention of L.plantarum T34(1×108 CFU/mouse/day)resulted in improved defecation and enhanced intestinal morphology.Mechanistic studies revealed that L.plantarum T34 strengthend the intestinal barrier through the upregulation of tight junction protein genes,specifically Claudin-1,as well as mucosal barrier proteins such as Muc2 and Muc3.Additionally,L.plantarum T34 modulated gut homeostasis by influencing the expression of aquaporin genes,levels of the gut neurotransmitter serotonin(5-HT),and oxidative stress markers.Furthermore,L.plantarum T34 effectively restored the composition of the gastrointestinal microbiota,addressing dysbiosis linked to constipation.These findings suggested that L.plantarum T34 may serve as a promising probiotic intervention for managing constipation,offering a safe and effective alternative to traditional treatments.
文摘In the present paper we have observed the effect of discontinuous gradient two-step Percoll centrifugation on depleting mature T lymphocytes from normal bone marrow. The pre-/post-Percoll percentage of CD34+, and Leu4+ cells in MNC was counted by using APAAP technique. As the two-step Percoll centrifugation is simple,and time saving, and decreases the incidence of contamination of cultured cells as well,This technique may be of value in serum-free culture of hematopoietic cells and immunological studies.
