目的:通过统计学分析,探究利福平耐药实时荧光定量核酸扩增技术(Xpert MTB/RIF)联合结核感染T细胞斑点试验(T-SPOT.TB)对脊柱结核早期诊断的临床价值。方法:通过样本获取,回顾性收集2021年12月至2024年9月于青岛市胸科医院有疑似脊柱结...目的:通过统计学分析,探究利福平耐药实时荧光定量核酸扩增技术(Xpert MTB/RIF)联合结核感染T细胞斑点试验(T-SPOT.TB)对脊柱结核早期诊断的临床价值。方法:通过样本获取,回顾性收集2021年12月至2024年9月于青岛市胸科医院有疑似脊柱结核临床表现的患者65例,经确诊分为脊柱结核组(32例)和非脊柱结核组组(33例)。所有研究对象进行脓液的抗酸杆菌涂片检测、病理组织Xpert MTB/RIF和外周血T-SPOT.TB检测比较各单一检测方法和联合检测的诊断阳性率、阴性率和准确率,采用灵敏度、特异度、阳性预测值、阴性预测值和受试者工作特征曲线对各检测方法的诊断价值进行评价。结果:T-SPOT.TB、Xpert MTB/RIF、T-SPOT.TB与Xpert MTB/RIF串并联检测的阳性率分别为93.75%、81.25%、78.13%和96.88%,远高于涂片抗酸染色的阳性率43.75%。并联检测的阳性率高于串联检测及各单一检测,差异有统计学意义(P 0.05)。T-SPOT.TB、Xpert MTB/RIF、T-SPOT.TB与Xpert MTB/RIF串并联检测的准确率分别为89.23%、90.77%、89.33%和90.77%,高于涂片抗酸染色的准确率63.08%,差异有统计学意义(P 0.05)。结论:T-SPOT.TB、Xpert MTB/RIF及其串并联检测用于脊柱结核的诊断相比涂片抗酸染色诊断效能更优,采用两者并联检测的效能最高,但若考虑成本效益,可选择Xpert MTB/RIF单一检测。Objective: To investigate the clinical value of combining Xpert MTB/RIF (rifampicin-resistant real-time fluorescence quantitative nucleic acid amplification detection technology) and T-SPOT.TB (Tuberculosis Infection T-Cell Spot Test) in the early diagnosis of spinal tuberculosis (STB) through statistical analysis. Methods: A retrospective collection was conducted from December 2021 to September 2024 at Qingdao Chest Hospital, involving 65 patients with clinical manifestations suggestive of spinal tuberculosis. After confirmation, the patients were divided into a spinal tuberculosis group (STB, 32 cases) and a non-spinal tuberculosis group (non-STB, 33 cases). All study subjects underwent acid-fast bacillus smear testing of pus, Xpert MTB/RIF testing of pathological tissues, and T-SPOT.TB testing of peripheral blood. The diagnostic positivity rate, negativity rate, and accuracy rate of each single detection method and combined detection were compared. The diagnostic value of each detection method was evaluated using sensitivity, specificity, positive predictive value, negative predictive value, and receiver operating characteristic (ROC) curve analysis. Results: The positive rates of T-SPOT.TB, Xpert MTB/RIF, T-SPOT.TB and Xpert MTB/RIF in series and parallel were 93.75%, 81.25%, 78.13% and 96.88%, respectively, which were significantly higher than that of acid-fast staining of sputum (43.75%). The positive rate of parallel detection was higher than that of series detection and each single detection, with statistically significant differences (P 0.05). The accuracy rates of T-SPOT.TB, Xpert MTB/RIF, T-SPOT.TB and Xpert MTB/RIF in series and parallel were 89.23%, 90.77%, 89.33% and 90.77%, respectively, which were higher than that of acid-fast staining of sputum (63.08%), with statistically significant differences (P 0.05). Conclusion: T-SPOT.TB, Xpert MTB/RIF and their series and parallel detections have better diagnostic efficacy for spinal tuberculosis than acid-fast staining of sputum. The efficacy of parallel detection of the two is the highest, but if cost-effectiveness is considered, Xpert MTB/RIF single detection can be selected.展开更多
在工业革命浪潮席卷欧洲的19世纪,技术与人性之间的张力逐渐成为文学书写的焦点。E. T. A.霍夫曼的短篇小说《自动机器人》以其独特的叙事实验,将工业时代人们狂热与焦虑的矛盾嵌入文本肌理,成为一面折射社会心理的棱镜。本文以小说中...在工业革命浪潮席卷欧洲的19世纪,技术与人性之间的张力逐渐成为文学书写的焦点。E. T. A.霍夫曼的短篇小说《自动机器人》以其独特的叙事实验,将工业时代人们狂热与焦虑的矛盾嵌入文本肌理,成为一面折射社会心理的棱镜。本文以小说中神秘的机械人偶“土耳其人”为切入点,基于后现代叙事理论框架,揭示文本如何映射过度关注技术对人性边界的冲击。从19世纪的自动机械到当代人工智能,霍夫曼的文学预言跨越时空,警示着技术进步对真实情感的侵蚀,并为反思人机共生的伦理困境提供了历史锚点。展开更多
文摘目的:通过统计学分析,探究利福平耐药实时荧光定量核酸扩增技术(Xpert MTB/RIF)联合结核感染T细胞斑点试验(T-SPOT.TB)对脊柱结核早期诊断的临床价值。方法:通过样本获取,回顾性收集2021年12月至2024年9月于青岛市胸科医院有疑似脊柱结核临床表现的患者65例,经确诊分为脊柱结核组(32例)和非脊柱结核组组(33例)。所有研究对象进行脓液的抗酸杆菌涂片检测、病理组织Xpert MTB/RIF和外周血T-SPOT.TB检测比较各单一检测方法和联合检测的诊断阳性率、阴性率和准确率,采用灵敏度、特异度、阳性预测值、阴性预测值和受试者工作特征曲线对各检测方法的诊断价值进行评价。结果:T-SPOT.TB、Xpert MTB/RIF、T-SPOT.TB与Xpert MTB/RIF串并联检测的阳性率分别为93.75%、81.25%、78.13%和96.88%,远高于涂片抗酸染色的阳性率43.75%。并联检测的阳性率高于串联检测及各单一检测,差异有统计学意义(P 0.05)。T-SPOT.TB、Xpert MTB/RIF、T-SPOT.TB与Xpert MTB/RIF串并联检测的准确率分别为89.23%、90.77%、89.33%和90.77%,高于涂片抗酸染色的准确率63.08%,差异有统计学意义(P 0.05)。结论:T-SPOT.TB、Xpert MTB/RIF及其串并联检测用于脊柱结核的诊断相比涂片抗酸染色诊断效能更优,采用两者并联检测的效能最高,但若考虑成本效益,可选择Xpert MTB/RIF单一检测。Objective: To investigate the clinical value of combining Xpert MTB/RIF (rifampicin-resistant real-time fluorescence quantitative nucleic acid amplification detection technology) and T-SPOT.TB (Tuberculosis Infection T-Cell Spot Test) in the early diagnosis of spinal tuberculosis (STB) through statistical analysis. Methods: A retrospective collection was conducted from December 2021 to September 2024 at Qingdao Chest Hospital, involving 65 patients with clinical manifestations suggestive of spinal tuberculosis. After confirmation, the patients were divided into a spinal tuberculosis group (STB, 32 cases) and a non-spinal tuberculosis group (non-STB, 33 cases). All study subjects underwent acid-fast bacillus smear testing of pus, Xpert MTB/RIF testing of pathological tissues, and T-SPOT.TB testing of peripheral blood. The diagnostic positivity rate, negativity rate, and accuracy rate of each single detection method and combined detection were compared. The diagnostic value of each detection method was evaluated using sensitivity, specificity, positive predictive value, negative predictive value, and receiver operating characteristic (ROC) curve analysis. Results: The positive rates of T-SPOT.TB, Xpert MTB/RIF, T-SPOT.TB and Xpert MTB/RIF in series and parallel were 93.75%, 81.25%, 78.13% and 96.88%, respectively, which were significantly higher than that of acid-fast staining of sputum (43.75%). The positive rate of parallel detection was higher than that of series detection and each single detection, with statistically significant differences (P 0.05). The accuracy rates of T-SPOT.TB, Xpert MTB/RIF, T-SPOT.TB and Xpert MTB/RIF in series and parallel were 89.23%, 90.77%, 89.33% and 90.77%, respectively, which were higher than that of acid-fast staining of sputum (63.08%), with statistically significant differences (P 0.05). Conclusion: T-SPOT.TB, Xpert MTB/RIF and their series and parallel detections have better diagnostic efficacy for spinal tuberculosis than acid-fast staining of sputum. The efficacy of parallel detection of the two is the highest, but if cost-effectiveness is considered, Xpert MTB/RIF single detection can be selected.
文摘在工业革命浪潮席卷欧洲的19世纪,技术与人性之间的张力逐渐成为文学书写的焦点。E. T. A.霍夫曼的短篇小说《自动机器人》以其独特的叙事实验,将工业时代人们狂热与焦虑的矛盾嵌入文本肌理,成为一面折射社会心理的棱镜。本文以小说中神秘的机械人偶“土耳其人”为切入点,基于后现代叙事理论框架,揭示文本如何映射过度关注技术对人性边界的冲击。从19世纪的自动机械到当代人工智能,霍夫曼的文学预言跨越时空,警示着技术进步对真实情感的侵蚀,并为反思人机共生的伦理困境提供了历史锚点。
