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基于.Net的System._ComObject类型辨析
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作者 骆文亮 陈伟 《信息技术》 2010年第1期85-86,107,共3页
针对在.NET平台下对COM组件进行交互编程时,遇到调用函数返回值的类型是System._ComObject而无法得到其真正类型的问题,以.NET框架与COM接口之间的通讯机制为出发点,研究和总结了System._ComObject产生的原因,最后给出了解决办法和应用... 针对在.NET平台下对COM组件进行交互编程时,遇到调用函数返回值的类型是System._ComObject而无法得到其真正类型的问题,以.NET框架与COM接口之间的通讯机制为出发点,研究和总结了System._ComObject产生的原因,最后给出了解决办法和应用实例。 展开更多
关键词 .NET框架 组件对象模型 运行时可调用包装 COM对象类型
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Diffuse Large B-Cell Lymphoma of the Central Nervous System. Immunophenotype, Clinicopathological Features and Differential Diagnosis
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作者 Mónica Belinda Romero-Guadarrama María Esther Gutiérrez Díaz-Ceballos +1 位作者 Fiacro Jiménez-Ponce Samantha Thingen-Velarde 《Open Journal of Pathology》 2012年第4期133-139,共7页
Background: Diffuse large B-cell lymphomas of the central nervous system (DLBCL CNS) represent less than 1% of all lymphomas and between 2% and 3% of all cerebral tumors. They occur in adults of 60 years of age or mor... Background: Diffuse large B-cell lymphomas of the central nervous system (DLBCL CNS) represent less than 1% of all lymphomas and between 2% and 3% of all cerebral tumors. They occur in adults of 60 years of age or more. The objective of this work is to describe the clinical-pathological characteristics, the immunophenotype and the differential diagnosis. Clinical Case: From the files of the surgical pathology unit we found four cases of primary diffuse large B cell lymphoma of the central nervous system in a 6-year period. Three corresponded to women over 47 years of age and the other to a 42-year-old man. The time of evolution was between 2 and 4 months. The symptoms were headache, blurred vision, hemiparesis, and seizures. Localization was in the pineal region, the frontal, parietal regions, and the right thalamus. Morphologically, large lymphoid cells with a diffuse growth pattern and necrosis were observed. Immunohistochemical markers, such as CD 20 and bcl2 were positive, one was positive to CD3. Expression of bcl6 and CD 10 was positive in one case, and MUM-1 was positive in three cases. All the cases were negative for Epstein-Barr virus. Conclusions: The diffuse large-B cell lymphoma of the central nervous system is rare. Its average age of presentation is at 60 years or older. The localization is in the pineal, frontal, parietal and thalamic regions. Three cases were originated by activated B lymphocyte (MUM-1 expression) and other from the Germinal Center (GC) (CD 10 expression). The clinical course was bad. The four patients died shortly after the diagnosis. 展开更多
关键词 PRIMARY LYMPHOMA CENTRAL Nervous system
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Application of Linear Thermodynamics to the Atmospheric System.Part Ⅱ: Exemplification of Linear Phenomenological Relations in the Atmospheric System
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作者 胡隐樵 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2002年第5期767-776,共10页
The linear phenomenological relations in the atmospheric boundary layer are proved indirectly using observational facts to combine linear thermodynamic theory and similarity theory in the boundary layer. Furthermore, ... The linear phenomenological relations in the atmospheric boundary layer are proved indirectly using observational facts to combine linear thermodynamic theory and similarity theory in the boundary layer. Furthermore, it is proved that Ihe turbulent transport coefficients are in proportion to Ihe corresponding linear phenomenological coefficients. But the experimental facts show that the linear phenomenological relations are not (tenable in the atmospheric mixing layer because the turbulenl transport process is an intense non-linear process in the mixing layer. Hence the convection boundary layer is a thermodynamic stale in a non-linear region far from the equilibrium state. The geostrophic wind is a special cross-coupling phenomenon between the dynamic process and the thermodynamic process in the atmospheric system. It is a practical exemplification of a cross-coupling phenomenon in the atmospheric system. 展开更多
关键词 atmospheric system linear thermodynamics linear phenomenological relations turbulent transportation coefficient atmospheric boundary layer
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Neural Modeling of Multivariable Nonlinear Stochastic System. Variable Learning Rate Case
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作者 Ayachi Errachdi Ihsen Saad Mohamed Benrejeb 《Intelligent Control and Automation》 2011年第3期167-175,共9页
The objective of this paper is to develop a variable learning rate for neural modeling of multivariable nonlinear stochastic system. The corresponding parameter is obtained by gradient descent method optimization. The... The objective of this paper is to develop a variable learning rate for neural modeling of multivariable nonlinear stochastic system. The corresponding parameter is obtained by gradient descent method optimization. The effectiveness of the suggested algorithm applied to the identification of behavior of two nonlinear stochastic systems is demonstrated by simulation experiments. 展开更多
关键词 NEURAL NETWORKS MULTIVARIABLE system STOCHASTIC Learning RATE Modeling
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Comparison Between Diltiazem and Cedilanid-D on Ventricular Rate Control of Atrial Fibrillation and Their Effect on Atrioventricular Conduction System.
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作者 朱立光 Mohammad +1 位作者 AL Mamun(穆罕默德·艾·马蒙) 《South China Journal of Cardiology》 CAS 2005年第2期113-117,共5页
Objectives This randomized study was designed to compare the safety and efficacy of intravenous diltiazem versus intravenous cedilanid-D (deslanoside) for ventricular rate control in patients with atrial fibrillati... Objectives This randomized study was designed to compare the safety and efficacy of intravenous diltiazem versus intravenous cedilanid-D (deslanoside) for ventricular rate control in patients with atrial fibrillation (AF). Analysis of the effect on conduction system of these drugs was also performed. Methods Forty three patients with AF were randomly assigned to receive intravenous therapy with 0.25mg/kg diltiazem (n = 21) or 0.4rag cedilanid-D (n = 22). If not effective at 120 minutes (〈 20% decrease in pretreatment ventricular rate or can not convert to sinus rhythm= another dose of diltiazem or 0.2mg cedilanid-D was administered. Blood pressure and electrocardiographic recordings were performed before and after 5, 10, 20, 30, 60 minutes of drug administration. Further recordings were performed at 120 minutes in noneffective patients, and at 180 minutes in patients who received second time drug administration. To evaluate the effect on conduction system of these two drugs by measuring PA, AH and HV intervals using His bundle electrogram test another nineteen sinus rhythm patients were randomized to dihiazem (n=9) and cedilanid (n= 10) group. His bundle electrogram recordings were performed before and after 5, 10, 20 and 30 minutes of drug administration. Statistical significance was assessed with the use of t test, Fisher's exact test, ANOVA and LSD methodology. Results At baseline and after 5, 10, 20, 30, 60 minutes of drug administration the heart rates (mean±SD) were(133±15), (92±20), (87±22), (85 ±20), (85±21), (85 ±23)beats/minute in diltiazem group respectively and( 140±21 ), ( 122±24), (118±25), (110±26), (112±25), (110±28) beats/ minute in cedilanid-D group respectively. Heart rate reduction was higher in diltiazem group than cedilanid group during 5 (41±20 vs 17±14,P 〈 0.01); 10 (46±21 vs 22±20, P〈0.01); 20 (48±21 vs 29±22, P〈0.01 ) ; 30(48±22 vs 27±22,P〈0.01 )and 60 minutes (48±23 vs 29±24, P〈 0.05). Both drugs had no effect on both systolic and diastolic blood pressure (P 〉0.05) and no major side effects were noticed. Diltiazem maintained effective ventrieular rate in 20 patients, whereas eedilanid-D maintained in 15 patients within 180 minutes (95.2%vs 68.2%,P〈 0.05). There were no statistical significance in baseline heart rate, age and weight between the two groups. Both diltiazem and cedilanid-D can increase AH interval, but have no effect on HV and PA intervals in sinus rhythm patients. Conclusions Both dihiazem and eedilanid-D decrease ventrieular heart rate, but heart rate reduction is significantly higher in diltiazem group, thus should be considered as a drug of choice for emergency control of ventrieular rate. Under clinical monitoring this dose of diltiazem seems to be safe and applicable in AF patients with congestive heart failure. Both drugs have no effect on PA and HV intervals but increase the AH interval thereby can reduce ventricular rate. 展开更多
关键词 Afrial fibrillation Atrioventricular conduction system Ventricular rate
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基于L-System与遗传算法的光线仿生采集在建筑设计中的应用研究
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作者 闵睿熙 张永 刘力 《贵阳学院学报(自然科学版)》 2025年第2期75-78,共4页
基于林登麦伊尔系统与遗传算法的光线仿生采集模型,通过模拟自然界中生物对光的利用方式,以此模拟采光效果。结果显示,所提模型可以看出不同时间段和不同测点的采光强度变化。晴天采光时14∶00至16∶00期间,5个测点的采光强度较高。阴... 基于林登麦伊尔系统与遗传算法的光线仿生采集模型,通过模拟自然界中生物对光的利用方式,以此模拟采光效果。结果显示,所提模型可以看出不同时间段和不同测点的采光强度变化。晴天采光时14∶00至16∶00期间,5个测点的采光强度较高。阴天的采光强度集中于12∶00至14∶00,其余时间采集的太阳强度相对较少,不利于采光。这说明,所提模型能够有效地预测建筑内不同区域的采光强度,并针对不同天气对光照条件进行合理利用。 展开更多
关键词 L-system 遗传算法 光线仿生采集 建筑光照优化
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System Xc-/GSH/GPX4通路介导铁死亡在消化道肿瘤耐药中的研究进展 被引量:1
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作者 李星霓 王潇 赵贵君 《河北医药》 2025年第4期663-667,共5页
获得性耐药是降低消化道肿瘤治疗疗效,引起肿瘤复发甚至死亡的主要原因,也是消化道肿瘤治疗的主要挑战。System Xc-/GSH/GPX4通路介导铁死亡与消化道肿瘤耐药性密切相关。了解System Xc/GSH/GPX4的结构和功能,探讨System Xc-/GSH/GPX4... 获得性耐药是降低消化道肿瘤治疗疗效,引起肿瘤复发甚至死亡的主要原因,也是消化道肿瘤治疗的主要挑战。System Xc-/GSH/GPX4通路介导铁死亡与消化道肿瘤耐药性密切相关。了解System Xc/GSH/GPX4的结构和功能,探讨System Xc-/GSH/GPX4通路在消化道肿瘤耐药中的作用,对于靶向System Xc-/GSH/GPX4通路及其相关调控因子,诱导铁死亡,克服肿瘤耐药性,提高肿瘤治疗疗效具有重要意义。本文通过阐述system Xc/GSH/GPX4的结构和功能,探讨System Xc-/GSH/GPX4通路在消化道肿瘤耐药中的作用,为进一步深入消化道肿瘤治疗提供新的思路及方向。 展开更多
关键词 system Xc-/GSH/GPX4 活性氧 铁死亡 消化道肿瘤 耐药
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Optimal Planning of Multiple PV-DG in Radial Distribution Systems Using Loss Sensitivity Analysis and Genetic Algorithm
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作者 A. Elkholy 《Journal of Power and Energy Engineering》 2025年第2期1-22,共22页
This paper introduces an optimized planning approach for integrating photovoltaic as distributed generation (PV-DG) into the radial distribution power systems, utilizing exhaustive load flow (ELF), loss sensitivity fa... This paper introduces an optimized planning approach for integrating photovoltaic as distributed generation (PV-DG) into the radial distribution power systems, utilizing exhaustive load flow (ELF), loss sensitivity factor (LSF), genetic algorithms (GA) methods, and numerical method based on LSF. The methodology aims to determine the optimal allocation and sizing of multiple PV-DG to minimize power loss through time series power flow analysis. An approach utilizing continuous sensitivity analysis is developed and inherently leverages power flow and loss equations to compute LSF of all buses in the system towards employing a dynamic PV-DG model for more accurate results. The algorithm uses a numerical grid search method to optimize PV-DG placement in a power distribution system, focusing on minimizing system losses. It combines iterative analysis, sensitivity assessment, and comprehensive visualization to identify and present the optimal PV-DG configurations. The present-ed algorithms are verified through co-simulation framework combining MATLAB and OpenDSS to carry out analysis for 12-bus radial distribution test system. The proposed numerical method is compared with other algorithms, such as ELF, LSF methods, and Genetic Algorithms (GA). Results show that the proposed numerical method performs well in comparison with LSF and ELF solutions. 展开更多
关键词 Photovoltaic systems Distributed Generation Multiple Allocation and Sizing Power Losses Radial Distribution system Genetic Algorithm
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Architecture to Secure Electrical Control System in Cyber-Physical System
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作者 Depeng Li 《Journal of Information Security》 2025年第1期149-157,共9页
It’s possible for malicious operators to seize hold of electrical control systems, for instance, the engine control unit of driverless vehicles, from various vectors, e.g. autonomic control system, remote vehicle acc... It’s possible for malicious operators to seize hold of electrical control systems, for instance, the engine control unit of driverless vehicles, from various vectors, e.g. autonomic control system, remote vehicle access, or human drivers. To mitigate potential risks, this paper provides the inauguration study by proposing a theoretical framework in the physical, human and cyber triad. Its goal is to, at each time point, detect adversary control behaviors and protect control systems against malicious operations via integrating a variety of methods. This paper only proposes a theoretical framework which tries to indicate possible threats. With the support of the framework, the security system can lightly reduce the risk. The development and implementation of the system are out of scope. 展开更多
关键词 ARCHITECTURE Control system FRAMEWORK
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Construction and Application of University Welcome System under the Background of Informatization
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作者 Rong Su 《Journal of Software Engineering and Applications》 2025年第2期67-75,共9页
The rapid advancement of information technology has promoted the development of informatization in universities. The freshmen welcome information system of universities is the first important system to showcase the di... The rapid advancement of information technology has promoted the development of informatization in universities. The freshmen welcome information system of universities is the first important system to showcase the digital level of the university to new students. With the expansion of the enrollment scale of universities, improving the efficiency of welcoming work has become an urgent problem to be solved. This article analyzes the characteristics and existing problems of the welcoming work, combined with the main technical methods of information system construction, and based on the comprehensive situation of information systems in our university, proposes the construction goals and ideas of the welcoming information system, summarizes the construction process and operation results of the welcome system, and explores possible directions for future optimization. 展开更多
关键词 Welcome system INFORMATIZATION UNIVERSITY Digital Campus REGISTRATION
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Reinforcement Learning in Mechatronic Systems: A Case Study on DC Motor Control
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作者 Alexander Nüßgen Alexander Lerch +5 位作者 René Degen Marcus Irmer Martin de Fries Fabian Richter Cecilia Boström Margot Ruschitzka 《Circuits and Systems》 2025年第1期1-24,共24页
The integration of artificial intelligence into the development and production of mechatronic products offers a substantial opportunity to enhance efficiency, adaptability, and system performance. This paper examines ... The integration of artificial intelligence into the development and production of mechatronic products offers a substantial opportunity to enhance efficiency, adaptability, and system performance. This paper examines the utilization of reinforcement learning as a control strategy, with a particular focus on its deployment in pivotal stages of the product development lifecycle, specifically between system architecture and system integration and verification. A controller based on reinforcement learning was developed and evaluated in comparison to traditional proportional-integral controllers in dynamic and fault-prone environments. The results illustrate the superior adaptability, stability, and optimization potential of the reinforcement learning approach, particularly in addressing dynamic disturbances and ensuring robust performance. The study illustrates how reinforcement learning can facilitate the transition from conceptual design to implementation by automating optimization processes, enabling interface automation, and enhancing system-level testing. Based on the aforementioned findings, this paper presents future directions for research, which include the integration of domain-specific knowledge into the reinforcement learning process and the validation of this process in real-world environments. The results underscore the potential of artificial intelligence-driven methodologies to revolutionize the design and deployment of intelligent mechatronic systems. 展开更多
关键词 Artificial Intelligence in Product Development Mechatronic systems Reinforcement Learning for Control system Integration and Verification Adaptive Optimization Processes Knowledge-Based Engineering
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Multi-Factor Authentication for Keyless Entry Systems: An Innovative Approach to Automotive Security
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作者 Danilo Brito Shakour Abuzneid 《Journal of Information Security》 2025年第1期78-100,共23页
Car manufacturers aim to enhance the use of two-factor authentication (2FA) to protect keyless entry systems in contemporary cars. Despite providing significant ease for users, keyless entry systems have become more s... Car manufacturers aim to enhance the use of two-factor authentication (2FA) to protect keyless entry systems in contemporary cars. Despite providing significant ease for users, keyless entry systems have become more susceptible to appealing attacks like relay attacks and critical fob hacking. These weaknesses present considerable security threats, resulting in unauthorized entry and car theft. The suggested approach combines a conventional keyless entry feature with an extra security measure. Implementing multi-factor authentication significantly improves the security of systems that allow keyless entry by reducing the likelihood of unauthorized access. Research shows that the benefits of using two-factor authentication, such as a substantial increase in security, far outweigh any minor drawbacks. 展开更多
关键词 MFA Keyless Entry systems Automotive Security Biometric Authentication
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Analysis of Two Irrigation Water Pumping Systems on the Taddis Valley Site: Solar Kit and Motor Pump Unit (GMP) in Tahoua (Niger)
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作者 Yerima Bako Djibo Aboubacar Guero Yadji Bori Haoua 《World Journal of Engineering and Technology》 2025年第1期39-48,共10页
The study was carried out in the Tahoua region at the market gardening sites of the Taddis 1 and 2 valley. Small-scale pumping irrigation is one of the most interesting uses of solar energy. The objective of this stud... The study was carried out in the Tahoua region at the market gardening sites of the Taddis 1 and 2 valley. Small-scale pumping irrigation is one of the most interesting uses of solar energy. The objective of this study is to carry out a comparative analysis of two dewatering pumping systems (Solar Kit and GMP) for water mobilization on a certain number of criteria such as sustainable use, economic aspect and performance. To achieve this, the adapted methodology consisted first of all in the development of a data collection tool in the field. Then flow measurements, estimation of fuel consumption, pressure height, etc., were carried out. Thus, the data collection involved a sample of 120 irrigators who had to use the two (2) types of pumping systems. The collected data were analyzed and processed with appropriate software. The results of the study show that the two pumping systems studied have strengths and constraints. Thus, the solar pumping system has a significant investment cost, very low maintenance and a low operating cost. On the other hand, the system with a generator has a relatively low investment cost (25 to 30 times less than solar), but a relatively high operating, upkeep and maintenance cost. He adds that these assets and constraints must be taken into consideration when an investment is made. This study shows that 74% of producers use GMP compared to 26% who use the Solar Kit. But in practice, the Solar Kit is more reliable for producers from the point of view of planted area, environmental management and investment costs, supply of fuel and lubricant. These results indicate better performance of the solar pumping system compared to GMP at the study sites. 展开更多
关键词 Market Gardening Sites Pumping systems Solar Kit GMP Taddis and Tahoua Valley
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Effectiveness of Logistics Management Information System (LMIS) in Improving the Availability of Essential Medicines and Medical Supplies in Public Hospitals in Zambia: A Cross-Sectional Study
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作者 Aubrey Kanyika Steward Mudenda +4 位作者 Victor Daka Nayuda Kaonga Chitundu Mbao Diana K. Mwaba Scott Kaba Matafwali 《Pharmacology & Pharmacy》 2025年第2期61-72,共12页
Background: The availability of essential medicines and medical supplies is crucial for effectively delivering healthcare services. In Zambia, the Logistics Management Information System (LMIS) is a key tool for manag... Background: The availability of essential medicines and medical supplies is crucial for effectively delivering healthcare services. In Zambia, the Logistics Management Information System (LMIS) is a key tool for managing the supply chain of these commodities. This study aimed to evaluate the effectiveness of LMIS in ensuring the availability of essential medicines and medical supplies in public hospitals in the Copperbelt Province of Zambia. Materials and Methods: From February to April 2022, a cross-sectional study was conducted in 12 public hospitals across the Copperbelt Province. Data were collected using structured questionnaires, checklists, and stock control cards. The study assessed LMIS availability, training, and knowledge among pharmacy personnel, as well as data accuracy, product availability, and order fill rates. Descriptive statistics were used to analyse the data. Results: All surveyed hospitals had LMIS implemented and were using eLMIS as the primary LMIS. Only 47% and 48% of pharmacy personnel received training in eLMIS and Essential Medicines Logistics Improvement Program (EMLIP), respectively. Most personnel demonstrated good knowledge of LMIS, with 77.7% able to log in to eLMIS Facility Edition, 76.6% able to locate stock control cards in the system, and 78.7% able to perform transactions. However, data accuracy from physical and electronic records varied from 0% to 60%, and product availability ranged from 50% to 80%. Order fill rates from Zambia Medicines and Medical Supplies Agency (ZAMMSA) were consistently below 30%. Discrepancies were observed between physical stock counts and eLMIS records. Conclusion: This study found that most hospitals in the Copperbelt Province of Zambia have implemented LMIS use. While LMIS implementation is high in the Copperbelt Province of Zambia, challenges such as low training levels, data inaccuracies, low product availability, and order fill rates persist. Addressing these issues requires a comprehensive approach, including capacity building, data quality improvement, supply chain coordination, and investment in infrastructure and human resources. Strengthening LMIS effectiveness is crucial for improving healthcare delivery and patient outcomes in Zambia. 展开更多
关键词 Logistics Management Information system Essential Medicines Medical Supplies Supply Chain Management Zambia
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Weighted Voting Ensemble Model Integrated with IoT for Detecting Security Threats in Satellite Systems and Aerial Vehicles
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作者 Raed Alharthi 《Journal of Computer and Communications》 2025年第2期250-281,共32页
Small-drone technology has opened a range of new applications for aerial transportation. These drones leverage the Internet of Things (IoT) to offer cross-location services for navigation. However, they are susceptibl... Small-drone technology has opened a range of new applications for aerial transportation. These drones leverage the Internet of Things (IoT) to offer cross-location services for navigation. However, they are susceptible to security and privacy threats due to hardware and architectural issues. Although small drones hold promise for expansion in both civil and defense sectors, they have safety, security, and privacy threats. Addressing these challenges is crucial to maintaining the security and uninterrupted operations of these drones. In this regard, this study investigates security, and preservation concerning both the drones and Internet of Drones (IoD), emphasizing the significance of creating drone networks that are secure and can robustly withstand interceptions and intrusions. The proposed framework incorporates a weighted voting ensemble model comprising three convolutional neural network (CNN) models to enhance intrusion detection within the network. The employed CNNs are customized 1D models optimized to obtain better performance. The output from these CNNs is voted using a weighted criterion using a 0.4, 0.3, and 0.3 ratio for three CNNs, respectively. Experiments involve using multiple benchmark datasets, achieving an impressive accuracy of up to 99.89% on drone data. The proposed model shows promising results concerning precision, recall, and F1 as indicated by their obtained values of 99.92%, 99.98%, and 99.97%, respectively. Furthermore, cross-validation and performance comparison with existing works is also carried out. Findings indicate that the proposed approach offers a prospective solution for detecting security threats for aerial systems and satellite systems with high accuracy. 展开更多
关键词 Intrusion Detection Cyber-Physical systems Drone Security Weighted Ensemble Voting Unmanned Vehicles Security Strategies
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Exploring Cyber Threat Intelligence into Land Administration Systems for Enhanced Cyber Resilience
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作者 Pierre-François Blin Trias Aditya +1 位作者 Purnama Budi Santosa Christophe Claramunt 《Journal of Geographic Information System》 2025年第1期45-65,共21页
The current global cybersecurity landscape, characterized by the increasing scale and sophistication of cyberattacks, underscores the importance of integrating Cyber Threat Intelligence (CTI) into Land Administration ... The current global cybersecurity landscape, characterized by the increasing scale and sophistication of cyberattacks, underscores the importance of integrating Cyber Threat Intelligence (CTI) into Land Administration Systems (LAS). LAS services involve requests and responses concerning public and private cadastral data, including credentials of parties, ownership, and spatial parcels. This study explores the integration of CTI in LAS to enhance cyber resilience, focusing on the unique vulnerabilities of LAS, such as sensitive data management and interconnection with other critical systems related to spatial data uses and changes. The approach employs a case study of a typical country-specific LAS to analyse structured vulnerabilities and their attributes to determine the degree of vulnerability of LAS through a quantitative inductive approach. The analysis results indicate significant improvements in identifying and mitigating potential threats through CTI integration, thus enhancing cyber resilience. These findings are crucial for policymakers and practitioners to develop robust cybersecurity strategies for LAS. 展开更多
关键词 Cyber Threat Intelligence Common Vulnerabilities and Exposures Geodata Land Administration systems Risk Assessment Spatial Cadastral Data
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System Xc-/GPX4/Nrf2介导的铁死亡对脓毒症大鼠肠道机械屏障的影响 被引量:5
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作者 马涛 黄玮玮 于湘友 《中华实用诊断与治疗杂志》 2024年第1期12-18,共7页
目的 探讨System Xc-/GPX4/Nrf2通路介导的铁死亡对脓毒症大鼠肠道机械屏障损伤程度及炎症状态的影响。方法 24只SPF级雄性大鼠随机分为假手术组、脓毒症组、铁死亡组、治疗组各6只。脓毒症组、铁死亡组、治疗组采用盲肠结扎穿孔法制备... 目的 探讨System Xc-/GPX4/Nrf2通路介导的铁死亡对脓毒症大鼠肠道机械屏障损伤程度及炎症状态的影响。方法 24只SPF级雄性大鼠随机分为假手术组、脓毒症组、铁死亡组、治疗组各6只。脓毒症组、铁死亡组、治疗组采用盲肠结扎穿孔法制备脓毒症大鼠模型,假手术组开腹游离盲肠后还纳关腹。造模即刻,治疗组皮下注射去铁胺20 mg/kg,铁死亡组腹腔注射铁死亡激活剂Erastin 20 mg/kg,假手术组和脓毒症组腹腔注射等量生理盐水,注射后4组均皮下注射37℃生理盐水50 mg/kg液体复苏。造模24 h大鼠麻醉后开腹,取十二指肠(空肠),机械法组织匀浆后收集细胞悬液,应用荧光探针法检测小肠平滑肌细胞活性氧(ROS);透射电镜下观察小肠上皮组织中线粒体形态。然后腹主动脉采血离心取血清,采用比色法检测Fe^(3+)、D-乳酸脱氢酶(D-LDH)水平,采用硫代巴比妥酸缩合法检测丙二醛(MDA)水平,采用ELISA法检测肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6水平。采血后处死大鼠,取小肠组织,采用HE染色观察组织病理学改变,采用免疫组织化学ABC法检测GPX4、Nrf2、Claudin-1阳性面积百分比,采用Western blot法检测GPX4、Nrf2、Claudin-1蛋白相对表达量。结果 (1)治疗组[(56 528±182)×10~5/mL]、脓毒症组[(67 964±844)×10~5/mL]、铁死亡组[(71 958±1 306)×10~5/mL]小肠平滑肌细胞ROS荧光强度均高于假手术组[(52 551±438)×10~5/mL](P<0.05),脓毒症组、铁死亡组高于治疗组(P<0.05),铁死亡组高于脓毒症组(P<0.05)。(2)假手术组小肠上皮组织线粒体形态完整、嵴丰富整齐、双膜结构存在且体积较大;脓毒症组线粒体体积变小,膜信号变强,线粒体嵴松散并发生溶解现象;治疗组线粒体体积较脓毒症组略有恢复,双膜结构部分存在,线粒体嵴少量溶解;铁死亡组线粒体体积较脓毒症组进一步缩小,膜密度增高,线粒体嵴发生溶解及消失。(3)脓毒症组、治疗组、铁死亡组血清Fe^(3+)含量低于假手术组(P<0.05),MDA、D-LDH水平均高于假手术组(P<0.05);脓毒症组、铁死亡组血清Fe^(3+)含量低于治疗组,MDA、D-LDH水平高于治疗组(P<0.05);铁死亡组血清Fe^(3+)含量低于脓毒症组,MDA、D-LDH水平高于脓毒症组(P<0.05)。(4)脓毒症组、治疗组、铁死亡组血清IL-1β、TNF-α、IL-6水平均高于假手术组(P<0.05),脓毒症组、铁死亡组高于治疗组(P<0.05),铁死亡组高于脓毒症组(P<0.05)。(5)假手术组小肠绒毛丰富规整、未见基底层分离及毛细血管充血;脓毒症组绒毛顶端出现溶解、破碎,炎症细胞浸润明显,毛细血管充血;治疗组绒毛顶端部分破裂情况略有好转,但仍有毛细血管充血、炎症细胞浸润;铁死亡组小肠绒毛已出现崩解、脱落,炎症组织浸润明显,部分延伸到绒毛两侧,黏膜与黏膜下层出现分离。(6)脓毒症组、治疗组、铁死亡组Nrf2阳性面积百分比大于假手术组(P<0.05),GPX4、Claudin-1阳性面积百分比小于假手术组(P<0.05);脓毒症组、铁死亡组Nrf2阳性面积百分比大于治疗组,GPX4、Claudin-1阳性面积百分比小于治疗组(P<0.05);铁死亡组Nrf2阳性面积百分比大于脓毒症组,GPX4、Claudin-1阳性面积百分比小于脓毒症组(P<0.05)。(7)脓毒症组、铁死亡组Nrf2蛋白相对表达量高于治疗组,GPX4、Claudin-1蛋白相对表达量均低于治疗组(P<0.05);铁死亡组Nrf2蛋白相对表达量高于脓毒症组,GPX4、Claudin-1蛋白相对表达量均低于脓毒症组(P<0.05)。结论 脓毒症大鼠模型肠道损伤中存在铁死亡现象;抑制System Xc-/GPX4/Nrf2通路介导的铁死亡,可改善脓毒症大鼠肠道机械屏障的损伤程度及炎症状态。 展开更多
关键词 脓毒症 肠道损伤 system Xc-/GPX4/Nrf2 铁死亡 大鼠
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基于System xc(-)-GSH-GPX4抗氧化功能轴探讨眼针改善脑缺血再灌注损伤大鼠神经元铁死亡的作用机制 被引量:10
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作者 曹亚荣 马贤德 +6 位作者 樊程程 张新宇 王奡 王圣澎 孙梦月 王哲 张威 《针刺研究》 CAS CSCD 北大核心 2024年第12期1239-1247,共9页
目的:观察眼针对急性脑缺血再灌注损伤(CIRI)大鼠脑缺血半暗带皮质组织中抗氧化功能轴系统XC[System xc(-)]-谷胱甘肽(GSH)-谷胱甘肽过氧化物酶4(GPX4)的影响,探讨眼针改善CIRI大鼠神经元铁死亡的作用机制。方法:SD大鼠随机分为假手术... 目的:观察眼针对急性脑缺血再灌注损伤(CIRI)大鼠脑缺血半暗带皮质组织中抗氧化功能轴系统XC[System xc(-)]-谷胱甘肽(GSH)-谷胱甘肽过氧化物酶4(GPX4)的影响,探讨眼针改善CIRI大鼠神经元铁死亡的作用机制。方法:SD大鼠随机分为假手术组、模型组、眼针组和抑制剂组,每组15只。改良线栓法复制CIRI大鼠模型;眼针组和抑制剂组针刺双侧“上焦区”“肝区”“下焦区”“肾区”,留针30 min,于脑缺血再灌注后30 min、12 h、24 h各施针1次。抑制剂组每次针刺前30 min腹腔注射GPX4抑制剂RSL3(10 mg/kg),共3次。改良Garcia JH评分法评估大鼠神经功能损伤程度;TTC染色观察脑组织梗死灶面积;HE染色观察缺血区脑组织形态;透射电子显微镜观察缺血区脑组织神经元线粒体结构;比色法检测缺血区脑组织丙二醛(MDA)、GSH、亚铁离子(Fe2+)含量;ELISA法检测活性氧(ROS)的活性;Western blot法检测缺血区脑组织溶质载体家族7成员11(SLC7A11)、溶质载体家族3成员2(SLC3A2)和GPX4的蛋白表达;实时荧光定量PCR法检测缺血区脑组织SLC7A11、GPX4的mRNA表达。结果:与假手术组比较,模型组大鼠改良Garcia JH神经功能损伤评分降低(P<0.01),缺血侧脑组织可见明显的灰白色梗死灶,HE染色示出现多个较大面积的典型脑梗死改变,线粒体超微结构呈典型铁死亡改变,缺血区脑组织Fe2+、MDA含量及ROS活性显著升高(P<0.01);GSH含量,SLC7A11、SLC3A2、GPX4蛋白及GPX4、SLC7A11mRNA表达显著降低(P<0.01)。与模型组比较,眼针组治疗后改良Garcia JH神经功能损伤评分升高(P<0.01),脑组织的梗死灶面积缩小,脑组织细胞损伤程度明显减轻,线粒体损伤减轻,缺血区脑组织Fe2+、MDA含量及ROS活性显著降低(P<0.01);GSH含量,SLC7A11、SLC3A2、GPX4蛋白及GPX4、SLC7A11 mRNA表达升高(P<0.01,P<0.05)。与眼针组比较,抑制剂组神经功能损伤评分降低(P<0.01),梗死灶面积增大,细胞损伤程度加重,线粒体损伤加重,缺血区脑组织Fe2+、MDA含量及ROS活性显著升高(P<0.01,P<0.05);GSH含量,SLC7A11、SLC3A2、GPX4蛋白及GPX4、SLC7A11 mRNA表达降低(P<0.01,P<0.05)。结论:眼针可通过调控System xc(-)-GSH-GPX4抗氧化功能轴改善CIRI大鼠神经元铁死亡。 展开更多
关键词 眼针 脑缺血再灌注损伤 铁死亡 system xc(-)-GSH-GPX4 抗氧化功能轴 缺血半暗带
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基于System Generator的卷积加速结构设计与实现
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作者 成鸿群 刘宜成 +2 位作者 涂海燕 徐金鹏 王广泰 《计算机应用与软件》 北大核心 2024年第4期224-227,274,共5页
为解决卷积神经网络中卷积运算耗时长、运算复杂的问题,针对卷积运算的并行性特征,提出一种基于分块的流水线加速方法,并基于该方法在System Generator上进行了电路设计。通过在FPGA(Field-programmable Gate Array)上进行实验验证,该... 为解决卷积神经网络中卷积运算耗时长、运算复杂的问题,针对卷积运算的并行性特征,提出一种基于分块的流水线加速方法,并基于该方法在System Generator上进行了电路设计。通过在FPGA(Field-programmable Gate Array)上进行实验验证,该设计模型能正确输出卷积运算结果;在结构和输入数据相同的情况下,该设计模型在计算速度上相比于普通CPU最高可加速258倍,相比于服务器级CPU提高了近40倍,具有良好的加速效果。 展开更多
关键词 卷积神经网络 卷积运算 system GENERATOR 现场可编程门阵列
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基于自由曲面和L-system的红掌生长模型
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作者 邹一波 褚诗贤 +1 位作者 葛艳 陈明 《湖南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第1期85-90,共6页
针对红掌花卉生长可视化中参数难以确定的问题进行研究,提出推导控制点的方法,建立红掌器官模型:采用Bezier曲线结合扫描成型的方法来构造叶柄模型,采用双三次NURBS曲线对佛焰苞进行建模;运用红掌的相关表型数据拟合生长函数,提出基于... 针对红掌花卉生长可视化中参数难以确定的问题进行研究,提出推导控制点的方法,建立红掌器官模型:采用Bezier曲线结合扫描成型的方法来构造叶柄模型,采用双三次NURBS曲线对佛焰苞进行建模;运用红掌的相关表型数据拟合生长函数,提出基于红掌生长规律的微分L-system,可有效模拟红掌的拓扑结构和生长过程;通过虚拟器官表示红掌器官的几何属性,降低微分L-system的复杂度。试验验证提出的方法对红掌各项生长指标拟合度可达0.89以上,并可对每个生长阶段的红掌进行模拟,能有效地对红掌的生长过程进行建模。 展开更多
关键词 红掌 自由曲面建模 L-system 生长函数 虚拟器官
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