Pure collagen is biocompatible but lacks inherent osteoinductive,osteoimmunomodulatory and antibacterial activities.To obtain collagen with these characteristics,we developed a novel methodology of doping bioactive el...Pure collagen is biocompatible but lacks inherent osteoinductive,osteoimmunomodulatory and antibacterial activities.To obtain collagen with these characteristics,we developed a novel methodology of doping bioactive elements into collagen through the synchronous self-assembly/mineralization(SSM)of collagen.In the SSM model,amorphous mineral nanoparticles(AMN)(amorphous SrCO3,amorphous Ag3PO4,etc.)stabilized by the polyampholyte,carboxymethyl chitosan(CMC),and collagen molecules were the primary components under acidic conditions.As the pH gradually increased,intrafibrillar mineralization occurred via the self-adaptive interaction between the AMNs and the collagen microfibrils,which were self-assembling;the AMNs wrapped around the microfibrils became situated in the gap zones of collagen and finally transformed into crystals.Srdoped collagen scaffolds(Sr-CS)promoted in vitro cell proliferation and osteogenic differentiation of rat bone marrow mesenchymal stromal cells(rBMSCs)and synergistically improved osteogenesis of rBMSCs by altering the macrophage response.Ag-doped collagen scaffolds(Ag-CS)exhibited in vitro antibacterial effects on S.aureus,as well as cell/tissue compatibility.Moreover,Sr-CS implanted into the calvarial defect of a rat resulted in improved bone regeneration.Therefore,the SSM model is a de novo synthetic strategy for doping bioactive elements into collagen,and can be used to fabricate multifunctional collagen scaffolds to meet the clinical challenges of encouraging osteogenesis,boosting the immune response and fighting severe infection in bone defects.展开更多
The significant role of the polyelectrolytic nature of non-collagenous proteins (NCPs) in regulating the in vivo mineralization of collagen provides important insights for scientists searching for analogues of NCPs to...The significant role of the polyelectrolytic nature of non-collagenous proteins (NCPs) in regulating the in vivo mineralization of collagen provides important insights for scientists searching for analogues of NCPs to achieve in vitro collagen mineralization. Polyampholyte carboxymethyl chitosan (CMC) has both carboxyl and amino groups, which allows it to act as a cationic or anionic polyelectrolyte below or above its isoelectric point (IP), respectively. In this study, CMC was employed as the analogue of NCPs to stabilize amorphous calcium phosphate (ACP) under acidic conditions (pH < 3.5) via the formation of CMC/ACP nanocomplexes. In the presence of both ACP nanoparticles and acid collagen molecules, ACP nanoparticles could be integrated into collagen fibrils during the process of collagen self-assembly and achieve intrafibrillar mineralization of collagen in vitro (i.e., synchronous self-assembly/mineralization (SSM) of collagen). This mode of mineralization is different from established mechanisms in which mineralization follows the self-assembly (MFS) of collagen. Thus, SSM provides a new strategy for developing materials from mineralized collagen scaffolds.展开更多
Electrochemical degradation performances of three non-steroidal anti-inflammatory drugs(NSAIDs),acetaminophen(ACT),aspirin(ASP)and ibuprofen(IBP),were investigated and compared in their alone and mixture conditions us...Electrochemical degradation performances of three non-steroidal anti-inflammatory drugs(NSAIDs),acetaminophen(ACT),aspirin(ASP)and ibuprofen(IBP),were investigated and compared in their alone and mixture conditions using Ti/SnO_(2)-Sb/La-PbO_(2).The pseudo-first-order degradation kinetics(k)order was k_(IBP-A)(0.110 min^(-1))>k_(ASP-A)(0.092 min^(-1))>k_(ACRT-A)(0.066 min^(-1))in their alone condition,while that was k_(ACT-M)(0.088 min^(-1))>k_(ASP-M)(0.063 min^(-1))>k_(IBP-M)(0.057 min^(-1)) in their mixture condition.The·OH apparent production rate constant of 5.23 mmol L^(-1)min^(-1)m^(-2) and an electrical energy per order(E_(EO)) value of 6.55 Wh/L could ensure the synchronous degradation of the NSAIDs mixture.The mineralization efficiency of NSAIDs mixture was 86.9%at 240 min with a mineralization current efficiency of 1.67%.Acetic acid and oxalic acid were the main products in the mineralization process for the both conditions.In the mixture condition,there were higher k values at lower initial concentrations and higher current density,while the presence of carbonate and humic acid inhibited their degradation.The results indicated electrochemical advanced oxidation process can effectively and synchronously mineralize NSAIDs mixture in wastewater.展开更多
The single-function agents with wide-spectrum activity which tend to disturb the ecological balance of oral cavity cannot satisfy dental treatment need.A multi-functional agent with specifically targeted killing prope...The single-function agents with wide-spectrum activity which tend to disturb the ecological balance of oral cavity cannot satisfy dental treatment need.A multi-functional agent with specifically targeted killing property and in situ remineralization is warranted for caries management.A novel multi-functional agent(8DSS-C8-P-113)consisting of three domains,i.e.,a non-specific antimicrobial peptide(AMP)(P-113),a competence stim-ulating peptide(C8),and an enhancing remineralization domain(8DSS),is fabricated and evaluated in this study.The findings demonstrates that 2μM mL^(-1)of 8DSS-C8-P-113 eliminates planktonic Streptococcus mutans(S.mutans)without disrupting the oral normal flora.At a concentration of 8μM mL^(-1),it exhibits the ability to prevent S.mutans’adhesion.Furthermore,8DSS-C8-P-113 self-assembles a hydrogel state at the higher con-centration of 16μM mL^(-1).This hydrogel self-adheres on the tooth surface,resisting acid attack,eradicating S.mutans’biofilm,and inducing mineralization in order to facilitate the repair of demineralized dental hard tissue.Its significant effectiveness in reducing the severity of dental caries is also demonstrated in vivo in a rat model.This study suggests that the multi-functional bioactive AMP 8DSS-C8-P-113 is a promising agent to specifically target pathogen,prevent tooth demineralization,and effectively induce in situ bio-mimic reminer-alization for the management of dental caries.展开更多
Protein nanocages are ideal templates for the bio-inspired fabrication of nanomaterials due to several advantageous properties. During the mineralization of nanoparticles （NPs） inside protein nanocages, most studies...Protein nanocages are ideal templates for the bio-inspired fabrication of nanomaterials due to several advantageous properties. During the mineralization of nanoparticles （NPs） inside protein nanocages, most studies have employed a common strategy： seed formation inside protein nanocages followed by seeded NP growth. However, the seed formation step is restricted to gentle reaction conditions to avoid damage to the protein nanocages, which may greatly limit the spectrum of seed materials used for NP growth. We put forward a simple route to circumvent such a limitation： encapsulation of a preformed NP as the seed via self-assembly, followed by the growth of an outer metal layer. Using such a method, we succeeded in mineralizing size-tunable Au NPs and Au@Ag core-shell NPs （〈10 nm in diameter） with narrow size distributions inside the virus-based NPs of simian virus 40. The present route enables the utilization of NPs synthesized under any conditions as the starting seeds for nanomaterial growth inside protein nanocages. Therefore, it potentially leads to novel bioinorganic chimeric nanomaterials with tailorable components and structures.展开更多
Nanocrystals provide a variety of size and shape-dependent properties with applications in a wide range of areas, gaining much attention in the past few years. However, due to the nature of the kinetic nanocrystal gro...Nanocrystals provide a variety of size and shape-dependent properties with applications in a wide range of areas, gaining much attention in the past few years. However, due to the nature of the kinetic nanocrystal growth, the procedures often require strict experimental conditions and the shape and size of nanocrystals are difficult to control. In such context, organic templates, which are artificially modified or synthesized, can direct inorganic nanocrystal nucleation and growth to achieve desired shape, size and ultimately properties. In this review article, two general categories of organic templates used for making inorganic nanomaterials are discussed:biotemplates(e.g., peptide, lipid, DNA, and capsid) and block copolymer templates(e.g., block copolymer micelles, star-like block copolymer unimicelles). The goal of this review is to bridge these gaps and help foster a greater awareness of the range and applicability of different organic templating techniques within the field of nanotechnology.展开更多
The control of synchronous structures on formation of superlarge stratabound ore deposits is immense. Based on studies of ore deposits in South Qinling, northern Guangdong, Langshan and other areas, three new ideas in...The control of synchronous structures on formation of superlarge stratabound ore deposits is immense. Based on studies of ore deposits in South Qinling, northern Guangdong, Langshan and other areas, three new ideas in comparison with examples of ore control of synchronous structures both in China and abroad are proposed: (i) multi order ore control of synchronous structures, which means that synchronous structures of different orders display different controls on ore deposits; (ii) synchronous structures in different stages of basin evolution display different controls on basin fluid system and ore forming system; (iii) synchronous structure accompanying hydrothermal mineralization as a preexisting weak surface in earth crust often reactivate in later tectono thermal event to be a channelway for magma or thermal fluids which superimposed on and reformed preexisting ore beds to form large or superlarge composite ore deposits.展开更多
The hierarchical Bouligand structure,ubiquitous in organisms and endowing natural creatures with exceptional performance attributes,stands as a prime example of nature's evolutionary prowess.Following the example ...The hierarchical Bouligand structure,ubiquitous in organisms and endowing natural creatures with exceptional performance attributes,stands as a prime example of nature's evolutionary prowess.Following the example of nature,the construction of biomimetic Bouligand structures will significantly propel advancements and innovations within the domain of biomedical and healthcare applications.In this review,we summarize cutting-edge research progress of biomimetic Bouligand architectures.Firstly,the natural Bouligand structures in animals,plants,and humans are introduced.On this basis,the relationship between properties and Bouligand structure is briefly discussed,including toughening mechanism,optical characteristics,and biological properties.Subsequently,the review details the construction strategies of the biomimetic Bouligand architectures,covering a variety of methods such as self-assembly,biomimetic mineralization,shear brushing,electrostatic spinning,and 3D printing.Finally,the utilization of biomimetic Bouligand architectures in biomedical and healthcare fields,especially for bone regeneration,tooth repair,body protection,and biosensor transmission,is discussed in detail.Despite the significant theoretical advantages of Bouligand structure,its feasibility in biomedical and healthcare applications still remains in its infancy.We eagerly anticipate the future development of biomimetic Bouligand architectures with superior performance,tailored to clinical scenarios and health needs,thereby fulfilling the grand vision of“inspiration from nature and giving back to life.”展开更多
基金financial support from the National Natural Science Foundation of China(No.31870947,No.81871782 and No.81701019)The Science&Technology Development Fund Planning Project of Tianjin for Higher Education(No.20140134)The Science&Technology Development Fund of Tianjin Education Commission for Higher Education(NO.2017KJ220).
文摘Pure collagen is biocompatible but lacks inherent osteoinductive,osteoimmunomodulatory and antibacterial activities.To obtain collagen with these characteristics,we developed a novel methodology of doping bioactive elements into collagen through the synchronous self-assembly/mineralization(SSM)of collagen.In the SSM model,amorphous mineral nanoparticles(AMN)(amorphous SrCO3,amorphous Ag3PO4,etc.)stabilized by the polyampholyte,carboxymethyl chitosan(CMC),and collagen molecules were the primary components under acidic conditions.As the pH gradually increased,intrafibrillar mineralization occurred via the self-adaptive interaction between the AMNs and the collagen microfibrils,which were self-assembling;the AMNs wrapped around the microfibrils became situated in the gap zones of collagen and finally transformed into crystals.Srdoped collagen scaffolds(Sr-CS)promoted in vitro cell proliferation and osteogenic differentiation of rat bone marrow mesenchymal stromal cells(rBMSCs)and synergistically improved osteogenesis of rBMSCs by altering the macrophage response.Ag-doped collagen scaffolds(Ag-CS)exhibited in vitro antibacterial effects on S.aureus,as well as cell/tissue compatibility.Moreover,Sr-CS implanted into the calvarial defect of a rat resulted in improved bone regeneration.Therefore,the SSM model is a de novo synthetic strategy for doping bioactive elements into collagen,and can be used to fabricate multifunctional collagen scaffolds to meet the clinical challenges of encouraging osteogenesis,boosting the immune response and fighting severe infection in bone defects.
基金financial support of the project from the National Natural Science Foundation of China (Nos. 31870947 and 81571016)
文摘The significant role of the polyelectrolytic nature of non-collagenous proteins (NCPs) in regulating the in vivo mineralization of collagen provides important insights for scientists searching for analogues of NCPs to achieve in vitro collagen mineralization. Polyampholyte carboxymethyl chitosan (CMC) has both carboxyl and amino groups, which allows it to act as a cationic or anionic polyelectrolyte below or above its isoelectric point (IP), respectively. In this study, CMC was employed as the analogue of NCPs to stabilize amorphous calcium phosphate (ACP) under acidic conditions (pH < 3.5) via the formation of CMC/ACP nanocomplexes. In the presence of both ACP nanoparticles and acid collagen molecules, ACP nanoparticles could be integrated into collagen fibrils during the process of collagen self-assembly and achieve intrafibrillar mineralization of collagen in vitro (i.e., synchronous self-assembly/mineralization (SSM) of collagen). This mode of mineralization is different from established mechanisms in which mineralization follows the self-assembly (MFS) of collagen. Thus, SSM provides a new strategy for developing materials from mineralized collagen scaffolds.
基金financially supported by the National Science Fund for Distinguished Young Scholars(No.51625801)the National Natural Science Foundation of China(Nos.51878169 and52000028)+2 种基金the Guangdong Innovation Team Project for Colleges and Universities(No.2016KCXTD023)Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2017)Guangdong Basic and Applied Basic Research Foundation(Nos.2019A1515110182 and 2019A1515110681)。
文摘Electrochemical degradation performances of three non-steroidal anti-inflammatory drugs(NSAIDs),acetaminophen(ACT),aspirin(ASP)and ibuprofen(IBP),were investigated and compared in their alone and mixture conditions using Ti/SnO_(2)-Sb/La-PbO_(2).The pseudo-first-order degradation kinetics(k)order was k_(IBP-A)(0.110 min^(-1))>k_(ASP-A)(0.092 min^(-1))>k_(ACRT-A)(0.066 min^(-1))in their alone condition,while that was k_(ACT-M)(0.088 min^(-1))>k_(ASP-M)(0.063 min^(-1))>k_(IBP-M)(0.057 min^(-1)) in their mixture condition.The·OH apparent production rate constant of 5.23 mmol L^(-1)min^(-1)m^(-2) and an electrical energy per order(E_(EO)) value of 6.55 Wh/L could ensure the synchronous degradation of the NSAIDs mixture.The mineralization efficiency of NSAIDs mixture was 86.9%at 240 min with a mineralization current efficiency of 1.67%.Acetic acid and oxalic acid were the main products in the mineralization process for the both conditions.In the mixture condition,there were higher k values at lower initial concentrations and higher current density,while the presence of carbonate and humic acid inhibited their degradation.The results indicated electrochemical advanced oxidation process can effectively and synchronously mineralize NSAIDs mixture in wastewater.
基金supported by a grant from the NSFC/RGC Joint Research Scheme sponsored by the Research Grants Council of the Hong Kong Special Administrative Region,China and the National Natural Science Foundation of China(Project No.N_HKU706/20 and No.82061160492)Human third molars were obtained from patients who provided written informed consent,and the study was approved by the Institutional Review Board of the University of Hong Kong/Hospital Authority Hong Kong West Cluster(IRB No:UW22-657)+1 种基金Human saliva was collected from a volunteer who provided written informed consent,which was approved by the Institutional Review Board of the University of Hong Kong/Hospital Authority Hong Kong West Cluster(IRB No:UW22-657)All animal-related operations in this work were approved by the Ethics Committee of Anhui Medical University,China(ref.no.LLSC20232002).
文摘The single-function agents with wide-spectrum activity which tend to disturb the ecological balance of oral cavity cannot satisfy dental treatment need.A multi-functional agent with specifically targeted killing property and in situ remineralization is warranted for caries management.A novel multi-functional agent(8DSS-C8-P-113)consisting of three domains,i.e.,a non-specific antimicrobial peptide(AMP)(P-113),a competence stim-ulating peptide(C8),and an enhancing remineralization domain(8DSS),is fabricated and evaluated in this study.The findings demonstrates that 2μM mL^(-1)of 8DSS-C8-P-113 eliminates planktonic Streptococcus mutans(S.mutans)without disrupting the oral normal flora.At a concentration of 8μM mL^(-1),it exhibits the ability to prevent S.mutans’adhesion.Furthermore,8DSS-C8-P-113 self-assembles a hydrogel state at the higher con-centration of 16μM mL^(-1).This hydrogel self-adheres on the tooth surface,resisting acid attack,eradicating S.mutans’biofilm,and inducing mineralization in order to facilitate the repair of demineralized dental hard tissue.Its significant effectiveness in reducing the severity of dental caries is also demonstrated in vivo in a rat model.This study suggests that the multi-functional bioactive AMP 8DSS-C8-P-113 is a promising agent to specifically target pathogen,prevent tooth demineralization,and effectively induce in situ bio-mimic reminer-alization for the management of dental caries.
基金Acknowledgements We greatly appreciate the financial support from the the National Natural Science Foundation of China （Nos. 31271076, 31470931 and 91527302） and the Key Research Program of the Chinese Academy of Sciences （No. KGZD-EW-T02-3）. We are grateful to Dr. D. Gao, B. C. Xu, P. Zhang and A. N. Du at the Center for Instrumental Analysis and Metrology, Wuhan Institute of Virology, CAS for assistance with TEM imaging and Dr. Kun Zhou at Suzhou Institute of Nano-Tech and Nano-Bionics, CAS for help with EDS analysis.
文摘Protein nanocages are ideal templates for the bio-inspired fabrication of nanomaterials due to several advantageous properties. During the mineralization of nanoparticles （NPs） inside protein nanocages, most studies have employed a common strategy： seed formation inside protein nanocages followed by seeded NP growth. However, the seed formation step is restricted to gentle reaction conditions to avoid damage to the protein nanocages, which may greatly limit the spectrum of seed materials used for NP growth. We put forward a simple route to circumvent such a limitation： encapsulation of a preformed NP as the seed via self-assembly, followed by the growth of an outer metal layer. Using such a method, we succeeded in mineralizing size-tunable Au NPs and Au@Ag core-shell NPs （〈10 nm in diameter） with narrow size distributions inside the virus-based NPs of simian virus 40. The present route enables the utilization of NPs synthesized under any conditions as the starting seeds for nanomaterial growth inside protein nanocages. Therefore, it potentially leads to novel bioinorganic chimeric nanomaterials with tailorable components and structures.
基金financialy supported by the National Key R&D Program of China(2017YFB0307600,to Xinchang Pang)Key R&D and Promotion Special Program of Henan Province(Grant No.2018-966,to Xinchang Pang)+1 种基金the 111 project(D18023)1000 Young Talent(to Xinchang Pang)
文摘Nanocrystals provide a variety of size and shape-dependent properties with applications in a wide range of areas, gaining much attention in the past few years. However, due to the nature of the kinetic nanocrystal growth, the procedures often require strict experimental conditions and the shape and size of nanocrystals are difficult to control. In such context, organic templates, which are artificially modified or synthesized, can direct inorganic nanocrystal nucleation and growth to achieve desired shape, size and ultimately properties. In this review article, two general categories of organic templates used for making inorganic nanomaterials are discussed:biotemplates(e.g., peptide, lipid, DNA, and capsid) and block copolymer templates(e.g., block copolymer micelles, star-like block copolymer unimicelles). The goal of this review is to bridge these gaps and help foster a greater awareness of the range and applicability of different organic templating techniques within the field of nanotechnology.
文摘The control of synchronous structures on formation of superlarge stratabound ore deposits is immense. Based on studies of ore deposits in South Qinling, northern Guangdong, Langshan and other areas, three new ideas in comparison with examples of ore control of synchronous structures both in China and abroad are proposed: (i) multi order ore control of synchronous structures, which means that synchronous structures of different orders display different controls on ore deposits; (ii) synchronous structures in different stages of basin evolution display different controls on basin fluid system and ore forming system; (iii) synchronous structure accompanying hydrothermal mineralization as a preexisting weak surface in earth crust often reactivate in later tectono thermal event to be a channelway for magma or thermal fluids which superimposed on and reformed preexisting ore beds to form large or superlarge composite ore deposits.
基金supported by National Natural Science Foundation of China(82371004)Fundamental Research Funds for the Central Universities(2042024YXB020).
文摘The hierarchical Bouligand structure,ubiquitous in organisms and endowing natural creatures with exceptional performance attributes,stands as a prime example of nature's evolutionary prowess.Following the example of nature,the construction of biomimetic Bouligand structures will significantly propel advancements and innovations within the domain of biomedical and healthcare applications.In this review,we summarize cutting-edge research progress of biomimetic Bouligand architectures.Firstly,the natural Bouligand structures in animals,plants,and humans are introduced.On this basis,the relationship between properties and Bouligand structure is briefly discussed,including toughening mechanism,optical characteristics,and biological properties.Subsequently,the review details the construction strategies of the biomimetic Bouligand architectures,covering a variety of methods such as self-assembly,biomimetic mineralization,shear brushing,electrostatic spinning,and 3D printing.Finally,the utilization of biomimetic Bouligand architectures in biomedical and healthcare fields,especially for bone regeneration,tooth repair,body protection,and biosensor transmission,is discussed in detail.Despite the significant theoretical advantages of Bouligand structure,its feasibility in biomedical and healthcare applications still remains in its infancy.We eagerly anticipate the future development of biomimetic Bouligand architectures with superior performance,tailored to clinical scenarios and health needs,thereby fulfilling the grand vision of“inspiration from nature and giving back to life.”
