Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approa...Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approaches or focused on a limited range of metabolites,leaving the regulatory mechanisms and dynamic metabolism of anthocyanins during ripening inadequately characterized.This study integrated anthocyanin-targeted metabolomics and transcriptomics to identify key anthocyanins in sweet cherry and construct a transcriptional regulatory network for anthocyanin biosynthesis.A novel bHLH transcription factor,Prunus avium bHLH transcription factor 102(PavbHLH102),was identified,and its role in regulating cyanidin levels was validated through overexpression and silencing experiments.Both in vitro and in vivo assays demonstrated that PavbHLH102 activates key anthocyanin biosynthetic genes,including PavF3H,PavDFR,and PavUFGT,thereby enhancing fruit coloration.Notably,PavF3′H upregulation significantly increased cyanidin accumulation.This study provides new insights into anthocyanin regulation in sweet cherry and offers valuable resources for improving fruit quality.展开更多
Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved i...Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.展开更多
China and Laos are close neighbors with a long-standing friendship.Since the early 2000s,China has supported Laos'economic and social development through wide-ranging cooperation projects,all guided by the vision ...China and Laos are close neighbors with a long-standing friendship.Since the early 2000s,China has supported Laos'economic and social development through wide-ranging cooperation projects,all guided by the vision of a community with a shared future.As this vision takes deeper root,many aid projects have moved from blueprint to reality,delivering tangible benefits across towns and villages and improving the lives of ordinary Lao people while further strengthening bilateral ties.展开更多
Lablab purpureus(L.)Sweet(L.purpureus)has been reported to alleviate diarrhea,although the precise mechanism remains unclear.This study identified the primary active components of L.purpureus utilizing ultraperformanc...Lablab purpureus(L.)Sweet(L.purpureus)has been reported to alleviate diarrhea,although the precise mechanism remains unclear.This study identified the primary active components of L.purpureus utilizing ultraperformance liquid chromatography/tandem mass spectrometry(UPLC-MS/MS).A rhubarb-induced diarrhea model in rats was utilized to assess the therapeutic efficacy of L.purpureus.Alterations in gut microbiota and fecal metabolism were analyzed via 16S r DNA analysis and targeted metabolomics.Flora elimination and fecal transplantation techniques were employed to deepen understanding of the role of intestinal flora in L.purpureus treatment.The study findings indicated that the main constituents of L.purpureus included trigonelline,piperidinic acid,and L-(–)-malic acid,among others.L.purpureus treatment significantly alleviated all diarrhea symptoms in rats,encompassing reduced fecal water content,weight loss,shortened colon length,diminished histological damage,and decreased inflammatory factors.Furthermore,L.purpureus significantly enhanced the expression of tight junction markers and restored the dysregulated intestinal flora in diarrheic rats by increasing Prevotella and reducing Lactobacillus.Additionally,the production of propionic acid and other short-chain fatty acids(SCFAs)increased in diarrheic rats treated with L.purpureus,suggesting a substantial alteration in the intestinal environment.Crucially,the protective efficacy of L.purpureus diminishes in the absence of gut flora.Subsequent fecal transplantation tests demonstrated that feces from the L.purpureus-treated group alleviated rhubarb-induced diarrhea,emphasizing the pivotal role of gut microbiota in the antidiarrheal efficacy of L.purpureus.In conclusion,our findings elucidate the underlying mechanisms of L.purpureus'antidiarrheal action and its beneficial impact on intestinal microflora.Moreover,these results provide compelling evidence supporting the therapeutic use of L.purpureus for the treatment of diarrhea and its associated complications.展开更多
Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of s...Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of sweets based on jujube, baobab fruit powder and sweet potato puree were produced, and their nutritional values and sensory properties such as taste, smell, color and acceptability were assessed. The formulation containing the most jujube powder, baobab fruit and sweet potato puree gave the best nutritional value. The sweets are rich in calcium (259.80 mg/100 g), magnesium (67.98 mg/100 g), potassium (782.04 mg/100 g), vitamin C (32.37 mg/100 g), iron (6.45 g mg/100 g) and zinc (0.97 mg/100 g). Sensory tests on the two formulations showed acceptability scores ranging from 81.26% to 84.58%, well above the minimum acceptable threshold. Candies with jujube, baobab fruit powder and sweet potato puree could be an alternative for preventing micronutrient deficiencies.展开更多
SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个...SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个SWEET成员,生物信息学分析表明金柑SWEET基因家族多数成员具有典型的7次跨膜螺旋结构,属于疏水碱性蛋白,在基序和结构域上相对保守。进化分析表明金柑SWEET成员可以分为4类。蛋白质互作网络分析发现FhSWEET16b是重要的核心家族成员。基因表达分析发现FhSWEET1、FhSWEET2b、FhSWEET15和FhSWEET16a在果实发育过程中表达量较高,可能在金柑果实糖分积累中起关键作用。本研究结果为进一步分析柑橘SWEET基因调控果实糖积累的分子机制提供基础。展开更多
Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and si...Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation.展开更多
Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sw...Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sweet spots.The formation of gas-bearing shales is closely linked to relative sealevel changes,providing an important approach to predicting sweet spots in the Wufeng-Longmaxi shale in the southern Sichuan Basin,China.Three types of marine shale gas sweet spots are identified in the shale based on their formation stages combined with relative sea-level changes:early,middle,and late transgression types.This study develops a prediction model and workflow for identifying shale gas sweet spots by analyzing relative sea-level changes and facies sequences.Predicting shale gas sweet spots in an explored block using this model and workflow can provide a valuable guide for well design and hydraulic fracturing,significantly enhancing the efficiency of shale gas exploration and development.Notably,the new prediction model and workflow can be utilized for the rapid evaluation of the potential for shale gas development in new shale gas blocks or those with low exploratory maturity.展开更多
Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of ei...Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of eight varieties of orange-fleshed of sweet potato (Ipomoea batatas (L.) to identify the most promising ones. The results show three groups of varieties with different agronomic performances. Group 1 composed of Irene and Vita is characterized by low production of small and large tuberous roots, low number of productive plants and low yield. Group 2 consisting mainly of Kabode is characterized by high production and weight of large tuberous roots as well as high yield. Group 3, composed of Tacha-2 Ininda, Ejumula Gloria, TIB-440060, CIP-199062-1, Bela bela is characterized by high production of small and large tuberous roots, high number of productive plants and average yield. The TIB-440060 variety has a recovery rate of 100%. The Irene, Kabode, Vita, Kabode varieties were resistant to viral and fungal diseases. In terms of technological quality, the TIB-440060 and CIP-199062-1 varieties have the best organoleptic qualities. Given the agronomic performances of the Kabode, TIB-440060 and CIP-199062-1 varieties, could be selected as the elites varieties and distributed to producers.展开更多
Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genom...Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genome assemblies contain unresolved gaps and comprise consensus chimeric sequences that fail to differentiate haplotype alleles,significantly constraining research on important agronomic trait inheritance.This study presents a phased-resolved telomere-to-telomere reference genome of sweet cherry‘Tieton’.The assembly anchors 653.03 Mb of sequence onto 16 pseudochromosomes representing two haplotypes,with 67,012 coding genes identified(33,777 in hapA and 33,235 in hapB).The genome demonstrates superior quality metrics,including a consensus accuracy exceeding QV44,contig N50 above 17.94 Mb,Benchmarking Universal Single-Copy Orthologs completeness of 98.7%,and a long terminal repeat(LTR)assembly index exceeding 20.This genome provides phased and annotated chromosome pairs,offering a comprehensive view of sweet cherry’s diploid genome organization.Utilizing this reference genome,we identified a large fragment deletion associated with yellow-skinned fruit in sweet cherry‘13-33’.This resource will significantly enhance breeding efforts and genetic research in sweet cherries.展开更多
基金funding from the Natural Science Foundation of Sichuan Province,China(Grant Nos.2025ZNSFSC1100,2024NSFSC0324)the National Key Research and Development Project(Grant No.2017YFC0505104)+2 种基金the Sichuan Agricultural University Dual Support Plan Special Project(Grant No.2024ZYTS020)the Postdoctoral Fellowship Program(Grade B)of China Postdoctoral Science Foundation(Grant No.GZB20250863)the China Postdoctoral Science Foundation(Grant No.2025M773740).
文摘Anthocyanins play a crucial role in shaping the visual appeal and nutritional quality of fruits.Previous research on anthocyanin biosynthesis in sweet cherry(Prunus avium L.)has primarily relied on single-omics approaches or focused on a limited range of metabolites,leaving the regulatory mechanisms and dynamic metabolism of anthocyanins during ripening inadequately characterized.This study integrated anthocyanin-targeted metabolomics and transcriptomics to identify key anthocyanins in sweet cherry and construct a transcriptional regulatory network for anthocyanin biosynthesis.A novel bHLH transcription factor,Prunus avium bHLH transcription factor 102(PavbHLH102),was identified,and its role in regulating cyanidin levels was validated through overexpression and silencing experiments.Both in vitro and in vivo assays demonstrated that PavbHLH102 activates key anthocyanin biosynthetic genes,including PavF3H,PavDFR,and PavUFGT,thereby enhancing fruit coloration.Notably,PavF3′H upregulation significantly increased cyanidin accumulation.This study provides new insights into anthocyanin regulation in sweet cherry and offers valuable resources for improving fruit quality.
基金supported by the National Natural Science Foundation of China(Grant Nos.32072630,32372774,and U22A20499)the earmarked fund for CARS(Grant No.CARS-19-01A).
文摘Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.
基金supported by the Yunnan Provincial Philosophy and Social Science Planning Projectthe Yunnan Academy of Social Sciences。
文摘China and Laos are close neighbors with a long-standing friendship.Since the early 2000s,China has supported Laos'economic and social development through wide-ranging cooperation projects,all guided by the vision of a community with a shared future.As this vision takes deeper root,many aid projects have moved from blueprint to reality,delivering tangible benefits across towns and villages and improving the lives of ordinary Lao people while further strengthening bilateral ties.
基金financial support from the State Key Research and Development Plan,China(2022YFD1801105)the 2115 Talent Development Program of China Agricultural University(00109015).
文摘Lablab purpureus(L.)Sweet(L.purpureus)has been reported to alleviate diarrhea,although the precise mechanism remains unclear.This study identified the primary active components of L.purpureus utilizing ultraperformance liquid chromatography/tandem mass spectrometry(UPLC-MS/MS).A rhubarb-induced diarrhea model in rats was utilized to assess the therapeutic efficacy of L.purpureus.Alterations in gut microbiota and fecal metabolism were analyzed via 16S r DNA analysis and targeted metabolomics.Flora elimination and fecal transplantation techniques were employed to deepen understanding of the role of intestinal flora in L.purpureus treatment.The study findings indicated that the main constituents of L.purpureus included trigonelline,piperidinic acid,and L-(–)-malic acid,among others.L.purpureus treatment significantly alleviated all diarrhea symptoms in rats,encompassing reduced fecal water content,weight loss,shortened colon length,diminished histological damage,and decreased inflammatory factors.Furthermore,L.purpureus significantly enhanced the expression of tight junction markers and restored the dysregulated intestinal flora in diarrheic rats by increasing Prevotella and reducing Lactobacillus.Additionally,the production of propionic acid and other short-chain fatty acids(SCFAs)increased in diarrheic rats treated with L.purpureus,suggesting a substantial alteration in the intestinal environment.Crucially,the protective efficacy of L.purpureus diminishes in the absence of gut flora.Subsequent fecal transplantation tests demonstrated that feces from the L.purpureus-treated group alleviated rhubarb-induced diarrhea,emphasizing the pivotal role of gut microbiota in the antidiarrheal efficacy of L.purpureus.In conclusion,our findings elucidate the underlying mechanisms of L.purpureus'antidiarrheal action and its beneficial impact on intestinal microflora.Moreover,these results provide compelling evidence supporting the therapeutic use of L.purpureus for the treatment of diarrhea and its associated complications.
文摘Increasing access to locally produced, safe, nutritious and affordable complementary foods is essential to combat micronutrient deficiencies in young children in low- and middle-income countries. Two formulations of sweets based on jujube, baobab fruit powder and sweet potato puree were produced, and their nutritional values and sensory properties such as taste, smell, color and acceptability were assessed. The formulation containing the most jujube powder, baobab fruit and sweet potato puree gave the best nutritional value. The sweets are rich in calcium (259.80 mg/100 g), magnesium (67.98 mg/100 g), potassium (782.04 mg/100 g), vitamin C (32.37 mg/100 g), iron (6.45 g mg/100 g) and zinc (0.97 mg/100 g). Sensory tests on the two formulations showed acceptability scores ranging from 81.26% to 84.58%, well above the minimum acceptable threshold. Candies with jujube, baobab fruit powder and sweet potato puree could be an alternative for preventing micronutrient deficiencies.
文摘SWEET(sugars will eventually be exported transporters)蛋白是近年来鉴定的新型糖转运蛋白,在植物生长发育和果实品质形成过程中有重要作用。为探究金柑SWEET基因家族特点及其在果实发育过程中的表达,从金柑基因组数据库中鉴定出17个SWEET成员,生物信息学分析表明金柑SWEET基因家族多数成员具有典型的7次跨膜螺旋结构,属于疏水碱性蛋白,在基序和结构域上相对保守。进化分析表明金柑SWEET成员可以分为4类。蛋白质互作网络分析发现FhSWEET16b是重要的核心家族成员。基因表达分析发现FhSWEET1、FhSWEET2b、FhSWEET15和FhSWEET16a在果实发育过程中表达量较高,可能在金柑果实糖分积累中起关键作用。本研究结果为进一步分析柑橘SWEET基因调控果实糖积累的分子机制提供基础。
基金funded by the National Key Research and Development Program of China(2018YFD1000104)the Shandong Provincial Fruit Industry Technology System Cultivation and Soil Fertilization Post,China(SDAIT-06-04)the Key R&D Program of Shandong Province(Competitive Innovation Platform),China(2022CXPT017).
文摘Bud endodormancy represents an ecological adaptation mechanism in perennial deciduous fruit trees to endure winter cold conditions.Sucrose serves a crucial role in bud endodormancy as both an energy metabolizer and signaling molecule.Sugars Will Eventually be Exported Transporters(SWEETs)function as sugar-efflux transporters that respond to environmental stimuli and contribute to plant growth and development.While SWEET gene families have been identified in various plant species for sugar transport regulation,their mechanism in regulating peach bud endodormancy remains undefined.In this study,we identified 15 SWEET genes in peach.The nomenclature was established through homologous alignment with the Arabidopsis SWEET gene family,resulting in four distinct clades through phylogenetic analysis.Covariance correlation analysis revealed 6 and 12 collinear SWEET genes in peach and Arabidopsis,respectively,forming 13 collinear gene pairs.Real-time quantitative polymerase chain reaction(RT-qPCR)analysis demonstrated significantly elevated expression of PpSWEET6 during peach bud endodormancy release,correlating positively with sucrose content.Transient overexpression of PpSWEET6 enhanced peach bud endodormancy release,while overexpressing PpSWEET6 in Arabidopsis enhanced seed germination and flowering.Y2H and luciferase complementation imaging(LCI)assays confirmed PpSWEET6 interacted with PpABF2.Additionally,dual luciferase reporter(DLR)assays showed that PpSWEET6 significantly decreased the activation of PpDAM6(key dormancy-inducing gene)through PpABF2,thereby modulating peach bud endodormancy release.These findings advance our understanding of SWEET genes in peach bud endodormancy regulation.
文摘Gas-bearing shales have become a major source of future natural gas production worldwide.It has become increasingly urgent to develop a reliable prediction model and corresponding workflow for identifying shale gas sweet spots.The formation of gas-bearing shales is closely linked to relative sealevel changes,providing an important approach to predicting sweet spots in the Wufeng-Longmaxi shale in the southern Sichuan Basin,China.Three types of marine shale gas sweet spots are identified in the shale based on their formation stages combined with relative sea-level changes:early,middle,and late transgression types.This study develops a prediction model and workflow for identifying shale gas sweet spots by analyzing relative sea-level changes and facies sequences.Predicting shale gas sweet spots in an explored block using this model and workflow can provide a valuable guide for well design and hydraulic fracturing,significantly enhancing the efficiency of shale gas exploration and development.Notably,the new prediction model and workflow can be utilized for the rapid evaluation of the potential for shale gas development in new shale gas blocks or those with low exploratory maturity.
文摘Sweet potato is one of the most important crops in Côte d’Ivoire. However, some constraints related to climate change limit its production. This study evaluated the agronomic and technological performances of eight varieties of orange-fleshed of sweet potato (Ipomoea batatas (L.) to identify the most promising ones. The results show three groups of varieties with different agronomic performances. Group 1 composed of Irene and Vita is characterized by low production of small and large tuberous roots, low number of productive plants and low yield. Group 2 consisting mainly of Kabode is characterized by high production and weight of large tuberous roots as well as high yield. Group 3, composed of Tacha-2 Ininda, Ejumula Gloria, TIB-440060, CIP-199062-1, Bela bela is characterized by high production of small and large tuberous roots, high number of productive plants and average yield. The TIB-440060 variety has a recovery rate of 100%. The Irene, Kabode, Vita, Kabode varieties were resistant to viral and fungal diseases. In terms of technological quality, the TIB-440060 and CIP-199062-1 varieties have the best organoleptic qualities. Given the agronomic performances of the Kabode, TIB-440060 and CIP-199062-1 varieties, could be selected as the elites varieties and distributed to producers.
基金supported by the Shandong Provincial Natural Science Foundation,China(ZR2021MC117)the Key R&D Program of Shandong Province,China(2022TZXD006).
文摘Sweet cherry(Prunus avium)represents a significant stone fruit crop in temperate regions worldwide.While molecular breeding has progressed substantially following the initial sweet cherry genome release,existing genome assemblies contain unresolved gaps and comprise consensus chimeric sequences that fail to differentiate haplotype alleles,significantly constraining research on important agronomic trait inheritance.This study presents a phased-resolved telomere-to-telomere reference genome of sweet cherry‘Tieton’.The assembly anchors 653.03 Mb of sequence onto 16 pseudochromosomes representing two haplotypes,with 67,012 coding genes identified(33,777 in hapA and 33,235 in hapB).The genome demonstrates superior quality metrics,including a consensus accuracy exceeding QV44,contig N50 above 17.94 Mb,Benchmarking Universal Single-Copy Orthologs completeness of 98.7%,and a long terminal repeat(LTR)assembly index exceeding 20.This genome provides phased and annotated chromosome pairs,offering a comprehensive view of sweet cherry’s diploid genome organization.Utilizing this reference genome,we identified a large fragment deletion associated with yellow-skinned fruit in sweet cherry‘13-33’.This resource will significantly enhance breeding efforts and genetic research in sweet cherries.
