The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from ten species of Chloranthus Swartz and two outgroup species of Sarcandra Gardn. were sequenced. The regions ranged in length from 654 to 663 b...The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from ten species of Chloranthus Swartz and two outgroup species of Sarcandra Gardn. were sequenced. The regions ranged in length from 654 to 663 bp, with ITS1 (285-290 bp) slightly longer than ITS2 (205-211 bp). Of the 669 aligned positions, 109 sites (56 in ITS1, 52 in ITS2, and one in 5.8S region) were phylogenetically informative. Only one most parsimonious tree of 252 steps was obtained in PAUP analysis when gaps were treated as the fifth state. The traditional division of Chloranthus on the basis of habit seems to be quite unnatural. Evidence from the sequence of ITS region, just as that from cytology and anatomy, strongly suggested the separation of the genus into two groups according to the characteristics of androecial organs: one contains C. angustifolius, C. nervosus, C. japonicus and C. fortunei, and the other comprises the remaining species.展开更多
Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations and phylogenetic relationships in genetic differentiation within 4 Chromium-treatment Leersia hexandra. The fresh leaves...Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations and phylogenetic relationships in genetic differentiation within 4 Chromium-treatment Leersia hexandra. The fresh leaves of Leersia hexandra cultivated on the condition of chrome pollution and exogenous organic acids were used as experimental material. The genomic DNA of Leersia hexandra was extracted by using CTAB method. The results showed that different samples of Leersia hexandra exhibited DNA polymorphism when using the random primer S43, S51and S55 as the primers in the RAPD reaction. One specific DNA band about 1000 bp was found in the sample which treated with 10 mmol/L concentration EDTA when used the S43 primer to RAPD. The obvious differences between different EDTA-treatment levels suggest that EDTA has certain effects on enrichment to heavy metals of Leersia hexandra, it will be more favored to Leersia hexandra accumulation of chromium when EDTA concentration increased.展开更多
Phytoextraction is a promising option for purifying hexavalent chromium(Cr(Ⅵ))-laden wastewater,but the long remediation period incurred by poor growth rate of Cr hyperaccumulators remains a primary hindrance to its ...Phytoextraction is a promising option for purifying hexavalent chromium(Cr(Ⅵ))-laden wastewater,but the long remediation period incurred by poor growth rate of Cr hyperaccumulators remains a primary hindrance to its large-scale application.In this study,we performed a hydroponic experiment to evaluate the feasibility of promoting the growth and phytoextraction efficiency of Cr hyperaccumulator Leersia hexandra Swartz(L.hexandra)by inoculating plant growth-promoting rhizobacteria(PGPR)Bacillus cereus(B.cereus).In batch tests,the Cr(Ⅵ)removal rates of L.hexandra and B.cereus co-culture were greater than the sum of their respective monocultures.This was likely due to the microbial reduction of Cr(Ⅵ)to Cr(Ⅲ),which is amiable to plant uptake.Besides,the PGPR factors of B.cereus,including indoleacetic acid(IAA)production,1-aminocyclopropane-1-carboxylic acid deamination(ACCd)activity,phosphate solubilization capacity,and siderophore production,were quantified.These PGPR factors helped explain the biomass augmentation,root elongation and enhanced Cr enrichment of the inoculated L.hexandra in pot experiments.Despite the increased Cr uptake,no aggravated oxidative damage to the cell membrane was observed in the inoculated L.hexandra.This was attributed to its capacity to confront the increased intracellular Cr stress by upregulating both the activities of antioxidative enzymes and expression of metal-binding proteins/peptides.Moreover,L.hexandra could always conserve the majority of Cr in the residual and oxalic integrated forms with low mobility and phytotoxicity,irrespective of the B.cereus inoculation.These results highlight the constructed Cr hyperaccumulatorrhizobacteria consortia as an effective candidate for decontaminating Cr(Ⅵ)-laden wastewater.展开更多
文摘The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from ten species of Chloranthus Swartz and two outgroup species of Sarcandra Gardn. were sequenced. The regions ranged in length from 654 to 663 bp, with ITS1 (285-290 bp) slightly longer than ITS2 (205-211 bp). Of the 669 aligned positions, 109 sites (56 in ITS1, 52 in ITS2, and one in 5.8S region) were phylogenetically informative. Only one most parsimonious tree of 252 steps was obtained in PAUP analysis when gaps were treated as the fifth state. The traditional division of Chloranthus on the basis of habit seems to be quite unnatural. Evidence from the sequence of ITS region, just as that from cytology and anatomy, strongly suggested the separation of the genus into two groups according to the characteristics of androecial organs: one contains C. angustifolius, C. nervosus, C. japonicus and C. fortunei, and the other comprises the remaining species.
文摘Randomly amplified polymorphic DNA (RAPD) technique was applied to assess the genetic variations and phylogenetic relationships in genetic differentiation within 4 Chromium-treatment Leersia hexandra. The fresh leaves of Leersia hexandra cultivated on the condition of chrome pollution and exogenous organic acids were used as experimental material. The genomic DNA of Leersia hexandra was extracted by using CTAB method. The results showed that different samples of Leersia hexandra exhibited DNA polymorphism when using the random primer S43, S51and S55 as the primers in the RAPD reaction. One specific DNA band about 1000 bp was found in the sample which treated with 10 mmol/L concentration EDTA when used the S43 primer to RAPD. The obvious differences between different EDTA-treatment levels suggest that EDTA has certain effects on enrichment to heavy metals of Leersia hexandra, it will be more favored to Leersia hexandra accumulation of chromium when EDTA concentration increased.
基金supported by the National Natural Science Foundation of China(Nos.52000046,52100034,52170154,and 52070051)the Special Project of Guangxi Science and Technology Base and Talent(Nos.GuiKe AD20297009 and GuiKe AD20297007)the Middle-aged and Young Teachers’Basic Ability Promotion Project of Guangxi(Nos.2020KY05039 and 2021KY0221).
文摘Phytoextraction is a promising option for purifying hexavalent chromium(Cr(Ⅵ))-laden wastewater,but the long remediation period incurred by poor growth rate of Cr hyperaccumulators remains a primary hindrance to its large-scale application.In this study,we performed a hydroponic experiment to evaluate the feasibility of promoting the growth and phytoextraction efficiency of Cr hyperaccumulator Leersia hexandra Swartz(L.hexandra)by inoculating plant growth-promoting rhizobacteria(PGPR)Bacillus cereus(B.cereus).In batch tests,the Cr(Ⅵ)removal rates of L.hexandra and B.cereus co-culture were greater than the sum of their respective monocultures.This was likely due to the microbial reduction of Cr(Ⅵ)to Cr(Ⅲ),which is amiable to plant uptake.Besides,the PGPR factors of B.cereus,including indoleacetic acid(IAA)production,1-aminocyclopropane-1-carboxylic acid deamination(ACCd)activity,phosphate solubilization capacity,and siderophore production,were quantified.These PGPR factors helped explain the biomass augmentation,root elongation and enhanced Cr enrichment of the inoculated L.hexandra in pot experiments.Despite the increased Cr uptake,no aggravated oxidative damage to the cell membrane was observed in the inoculated L.hexandra.This was attributed to its capacity to confront the increased intracellular Cr stress by upregulating both the activities of antioxidative enzymes and expression of metal-binding proteins/peptides.Moreover,L.hexandra could always conserve the majority of Cr in the residual and oxalic integrated forms with low mobility and phytotoxicity,irrespective of the B.cereus inoculation.These results highlight the constructed Cr hyperaccumulatorrhizobacteria consortia as an effective candidate for decontaminating Cr(Ⅵ)-laden wastewater.
