The hot compression tests of 7Mo super austenitic stainless(SASS)were conducted to obtain flow curves at the temperature of 1000-1200℃and strain rate of 0.001 s^(-1)to 1 s^(-1).To predict the non-linear hot deformati...The hot compression tests of 7Mo super austenitic stainless(SASS)were conducted to obtain flow curves at the temperature of 1000-1200℃and strain rate of 0.001 s^(-1)to 1 s^(-1).To predict the non-linear hot deformation behaviors of the steel,back propagation-artificial neural network(BP-ANN)with 16×8×8 hidden layer neurons was proposed.The predictability of the ANN model is evaluated according to the distribution of mean absolute error(MAE)and relative error.The relative error of 85%data for the BP-ANN model is among±5%while only 42.5%data predicted by the Arrhenius constitutive equation is in this range.Especially,at high strain rate and low temperature,the MAE of the ANN model is 2.49%,which has decreases for 18.78%,compared with conventional Arrhenius constitutive equation.展开更多
The development of flowers in soybean(Glycine max)is essential for determining the yield potential of the plant.Gene silencing pathways are involved in modulating flower development,but their full elucidation is still...The development of flowers in soybean(Glycine max)is essential for determining the yield potential of the plant.Gene silencing pathways are involved in modulating flower development,but their full elucidation is still incomplete.Here,we conducted a forward genetic screen and identified an abnormal flower mutant,deformed floral bud1-1(Gmdfb1-1),in soybean.We mapped and identified the causal gene,which encodes a member of the armadillo(ARM)-repeat superfamily.Using small RNA sequencing(sRNA-seq),we found an abnormal accumulation of small interfering RNAs(si RNAs)and microRNA(miRNAs)in the Gmdfb1 mutants.We further demonstrated that GmDFB1 interacts with the RNA exosome cofactor SUPER KILLER7(Gm SKI7).Additionally,GmDFB1 interacts with the PIWI domain of ARGONAUTE 1(GmAGO1)to inhibit the cleavage efficiency on the target genes of s RNAs.The enhanced gene silencing mediated by siRNA and miRNA in the Gmdfb1 mutants leads to the downregulation of their target genes associated with flower development.This study revealed the crucial role of GmDFB1 in regulating floral organ identity in soybean probably by participating in two distinct gene silencing pathways.展开更多
文摘The hot compression tests of 7Mo super austenitic stainless(SASS)were conducted to obtain flow curves at the temperature of 1000-1200℃and strain rate of 0.001 s^(-1)to 1 s^(-1).To predict the non-linear hot deformation behaviors of the steel,back propagation-artificial neural network(BP-ANN)with 16×8×8 hidden layer neurons was proposed.The predictability of the ANN model is evaluated according to the distribution of mean absolute error(MAE)and relative error.The relative error of 85%data for the BP-ANN model is among±5%while only 42.5%data predicted by the Arrhenius constitutive equation is in this range.Especially,at high strain rate and low temperature,the MAE of the ANN model is 2.49%,which has decreases for 18.78%,compared with conventional Arrhenius constitutive equation.
基金supported by the National Key Research and Development Program of China(Grant Nos.2021YFF1001201 and 2022YFF1001601-4)Key Research and Development Program of Shandong Province(Grant Nos.2023LZGC008 and 2021LZGC003)+7 种基金the National Scientific and Technological Innovation 2030-Major Project(Grant No.2023ZD040360102)the Joint Funds of the National Natural Science Foundation of China(Grant No.U1906203)the National Transgenic Project of China(Grant Nos.2016ZX08010002-009 and 2018ZX08009-14B)Taishan Scholar Youth Project of Shandong Province(Grant No.tsqn202306072)Young Scientists Fund of the National Natural Science Foundation of China(Grant No.32301876)Natural Science Foundation of Shandong Province Youth Fund(Grant No.ZR2023QC180)Qingdao Natural Science Foundation(Grant No.23-2-1-38-zyyd-jch)Shandong University Qilu Young Scholar。
文摘The development of flowers in soybean(Glycine max)is essential for determining the yield potential of the plant.Gene silencing pathways are involved in modulating flower development,but their full elucidation is still incomplete.Here,we conducted a forward genetic screen and identified an abnormal flower mutant,deformed floral bud1-1(Gmdfb1-1),in soybean.We mapped and identified the causal gene,which encodes a member of the armadillo(ARM)-repeat superfamily.Using small RNA sequencing(sRNA-seq),we found an abnormal accumulation of small interfering RNAs(si RNAs)and microRNA(miRNAs)in the Gmdfb1 mutants.We further demonstrated that GmDFB1 interacts with the RNA exosome cofactor SUPER KILLER7(Gm SKI7).Additionally,GmDFB1 interacts with the PIWI domain of ARGONAUTE 1(GmAGO1)to inhibit the cleavage efficiency on the target genes of s RNAs.The enhanced gene silencing mediated by siRNA and miRNA in the Gmdfb1 mutants leads to the downregulation of their target genes associated with flower development.This study revealed the crucial role of GmDFB1 in regulating floral organ identity in soybean probably by participating in two distinct gene silencing pathways.
