期刊文献+
共找到3,071篇文章
< 1 2 154 >
每页显示 20 50 100
m^(5)C RNA甲基化修饰与前庭功能异常和老年性耳聋的相关性研究
1
作者 周旭 司峰志 《河北医学》 2026年第1期117-122,共6页
目的:探讨m^(5)C RNA甲基化修饰在前庭功能和老年性耳聋中的相关性。方法:总计24只近交系C57BL/6J小鼠,5月龄(成年小鼠组)、12月龄(中年小鼠组)、20月龄(老年小鼠组)各8只。听觉脑干反应阈值分析评价小鼠听力。DCFH-DA(二氯二氢荧光素-... 目的:探讨m^(5)C RNA甲基化修饰在前庭功能和老年性耳聋中的相关性。方法:总计24只近交系C57BL/6J小鼠,5月龄(成年小鼠组)、12月龄(中年小鼠组)、20月龄(老年小鼠组)各8只。听觉脑干反应阈值分析评价小鼠听力。DCFH-DA(二氯二氢荧光素-乙酰乙酸酯)法测定耳蜗和前庭组织ROS水平。TAMRA探针法测定其中组织线粒体DNA的完整性。m^(5)C RNA甲基化修饰免疫共沉淀法分析小鼠耳蜗和前庭组织GRM7 mRNA m^(5)C水平。Western blot法测定其中NSUN2的水平。结果:与成年小鼠组和中年小鼠组相比,老年小鼠组在8、16和32 kHz时的平均ABR阈值升高(P<0.05);耳蜗组织和前庭组织ROS相对水平均升高(P<0.05);含CD mtDNA占比(%)均降低(P<0.05);NSUN2相对表达水平均降低(P<0.05);GRM7 Input mRNA相对表达水平均降低(P<0.05);GRM7 m^(5)C mRNA相对表达水平降低(P<0.05)。老年小鼠组,与耳蜗组织组相比,前庭组织组ROS相对水平较低(P<0.05),GRM7 Input mRNA和GRM7 m^(5)C mRNA相对表达水平较高(P<0.05)。结论:m^(5)C甲基化修饰RNA水平下调及NSUN2表达水平降低与前庭功能和老年性耳聋相关。 展开更多
关键词 老年性耳聋 前庭功能 C57BL/6J小鼠 m^(5)C RNA甲基化修饰 NOP2/Sun RNA甲基转移酶2
暂未订购
基于TCGA数据库资料评估头颈鳞状细胞癌患者Klotho基因表达与临床预后的关系
2
作者 张学聪 陈秉辉 +2 位作者 徐常艳 常方远 曹华 《中国耳鼻咽喉头颈外科》 2025年第1期48-50,共3页
目的探讨基于癌症基因组图谱(TCGA)数据库资料评估头颈鳞状细胞癌(head neck squamous cell carcinoma,HNSCC)患者Klotho基因表达对临床预后的影响。方法研究通过检索TCGA数据库收集HNSCC患者Klotho基因、微管相关蛋白轻链3(Microtubule... 目的探讨基于癌症基因组图谱(TCGA)数据库资料评估头颈鳞状细胞癌(head neck squamous cell carcinoma,HNSCC)患者Klotho基因表达对临床预后的影响。方法研究通过检索TCGA数据库收集HNSCC患者Klotho基因、微管相关蛋白轻链3(Microtubule-associated protein light chain 3,MAPLC3)、NOP2/Sun RNA甲基转移酶2(NOP2/Sun RNA methyltransferase 2,NSUN2)的表达及随访预后资料,根据Klotho基因表达水平分为低表达组(n=87例)、中表达组(n=87例)和高表达组(n=88例)。描绘Kaplan-Meier曲线分析Klotho基因表达对临床预后的影响,同时评价Klotho基因表达与MAPLC3及NSUN2的相关性。结果不同组别患者一般临床特征资料比较差异无统计学意义(P>0.05)。高表达组患者中位总生存率(overall survival,OS)显著高于低表达组(P<0.05)。与高表达组相比,低表达组风险比为0.62(95%CI:0.44~0.87,P<0.05)。HNSCC患者中Klotho基因与MAPLC3表达水平呈正相关(r=0.19,P=0.00),而与NSUN2表达水平呈负相关(r=-0.17,P=0.00)。结论Klotho基因表达与HNSCC患者临床预后有关,同时该基因可能影响下游MAPLC3及NSUN2的表达。 展开更多
关键词 头颈鳞状细胞癌(Squamous Cell Carcinoma of Head and Neck) 预后(Prognosis) Klotho基因(Klotho Gene) 微管相关蛋白轻链3(Microtubule-associated protein light chain 3) NOP2/Sun RNA甲基转移酶2(NOP2/Sun RNA methyltransferase 2)
暂未订购
NSUN2通过介导ARMC9的m5C修饰促进胃癌细胞的增殖、转移和侵袭
3
作者 李越 陈栋 +4 位作者 王瑾 彭怡 张远琦 杨芬 王学军 《中国药科大学学报》 北大核心 2025年第5期583-591,共9页
为研究NOP2/Sun RNA甲基转移酶2(NOP2/Sun RNA methyltransferase 2,NSUN2)对胃癌恶性进展的影响及其潜在机制,使用TCGA数据库分析发现胃癌组织中NSUN2表达水平显著升高;Western blot发现,与胃黏膜上皮细胞相比,NSUN2在胃癌细胞中表达上... 为研究NOP2/Sun RNA甲基转移酶2(NOP2/Sun RNA methyltransferase 2,NSUN2)对胃癌恶性进展的影响及其潜在机制,使用TCGA数据库分析发现胃癌组织中NSUN2表达水平显著升高;Western blot发现,与胃黏膜上皮细胞相比,NSUN2在胃癌细胞中表达上调;集落形成实验显示,NSUN2过表达细胞中集落形成能力增高;Transwell实验也证实NSUN2高表达时,发生迁移和侵袭的细胞数量显著增多。进一步通过数据库分析推测ARMC可能是NSUN2的下游靶分子,接着通过MeRIP-qPCR揭示NSUN2高表达可以提高ARMC9的m5C修饰水平,并且降低ARMC9 mRNA的降解速率,从而提高其蛋白表达水平。此外,过表达ARMC9可以增强细胞的集落形成能力和迁移侵袭能力。上述结果显示,NSUN2可以通过提高ARMC9 mRNA的m5C修饰水平,提高其稳定性,增强其表达,从而促进胃癌进展,因此NSUN2和ARMC9可以作为胃癌进展的潜在治疗靶标。 展开更多
关键词 5-甲基胞嘧啶 甲基转移酶 NOP2/Sun RNA甲基转移酶2 犰狳重复序列9 胃癌 肿瘤标志物
在线阅读 下载PDF
SUN2调控稳定表达绵羊肺腺瘤病毒囊膜蛋白的OAR-L1细胞的恶性转化能力的研究
4
作者 鲍安玉 杜晓悦 +3 位作者 陈思旭 段续接 张培 刘淑英 《中国兽医科学》 北大核心 2025年第5期669-677,共9页
为了探究SAD1/UN84结构域蛋白-2(SAD1/UNC84 domain protein-2,SUN2)在绵羊肺腺瘤病(ovine pulmonary adenocarcnoma,OPA)肿瘤细胞恶性转化中的功能,本试验利用实验室保存的JSRV env慢病毒感染绵羊肺成纤维细胞(OAR-L1),建立了OAR-L1-En... 为了探究SAD1/UN84结构域蛋白-2(SAD1/UNC84 domain protein-2,SUN2)在绵羊肺腺瘤病(ovine pulmonary adenocarcnoma,OPA)肿瘤细胞恶性转化中的功能,本试验利用实验室保存的JSRV env慢病毒感染绵羊肺成纤维细胞(OAR-L1),建立了OAR-L1-Env稳转株。通过qPCR和Western-blot检测JSRV Env和SUN2的表达情况,使用CCK-8、划分愈合试验和软琼脂糖集落形成试验检测细胞的恶性转化能力。此外,分别构建SUN2过表达和干扰质粒,并转染OAR-L1-Env稳转株,利用qPCR和Western-blot检测其SUN2的表达情况,通过CCK-8和划分愈合试验检测细胞的增殖和迁移能力。结果显示,OAR-L1-Env稳转株成功建立,且SUN2表达量显著下调(P<0.05),CCK-8、划分愈合试验和软琼脂糖集落形成试验表明OAR-L1-Env稳转株发生恶性转化。OAR-L1-Env稳转株中过表达SUN2可抑制细胞的增殖和迁移能力(P<0.05),而干扰SUN2则促进细胞的增殖和迁移能力(P<0.05)。综上所述,本研究表明SUN2参与调控JSRV Env诱导的细胞恶性转化,提示其可能对OPA肿瘤生成有抑制作用。本研究为进一步探究SUN2在OPA中的细胞增殖等生物学作用的研究提供了一定的参考资料。 展开更多
关键词 稳转株 囊膜蛋白 SUN2 增殖 迁移
在线阅读 下载PDF
Tests of Solar X-Ray Image Reconstruction:A New Index for Assessing Image Quality 被引量:1
5
作者 Zhen-Tong Li Wen-Hui Yu +2 位作者 Yang Su Wei Chen Wei-Qun Gan 《Research in Astronomy and Astrophysics》 2025年第3期76-89,共14页
Indirect X-ray modulation imaging has been adopted in a number of solar missions and provided reconstructed X-ray images of solar flares that are of great scientific importance.However,the assessment of the image qual... Indirect X-ray modulation imaging has been adopted in a number of solar missions and provided reconstructed X-ray images of solar flares that are of great scientific importance.However,the assessment of the image quality of the reconstruction is still difficult,which is particularly useful for scheme design of X-ray imaging systems,testing and improvement of imaging algorithms,and scientific research of X-ray sources.Currently,there is no specified method to quantitatively evaluate the quality of X-ray image reconstruction and the point-spread function(PSF)of an X-ray imager.In this paper,we propose percentage proximity degree(PPD)by considering the imaging characteristics of X-ray image reconstruction and in particular,sidelobes and their effects on imaging quality.After testing a variety of imaging quality assessments in six aspects,we utilized the technique for order preference by similarity to ideal solution to the indices that meet the requirements.Then we develop the final quality index for X-ray image reconstruction,QuIX,which consists of the selected indices and the new PPD.QuIX performs well in a series of tests,including assessment of instrument PSF and simulation tests under different grid configurations,as well as imaging tests with RHESSI data.It is also a useful tool for testing of imaging algorithms,and determination of imaging parameters for both RHESSI and ASO-S/Hard X-ray Imager,such as field of view,beam width factor,and detector selection. 展开更多
关键词 SUN flares-Sun X-rays gamma-rays-techniques image processing
在线阅读 下载PDF
Improving Image Quality of the Solar Disk Imager(SDI)of the LyαSolar Telescope(LST)Onboard the ASO-S Mission 被引量:1
6
作者 Hui Liu Hui Li +11 位作者 Sizhong Zou Kaifan Ji Zhenyu Jin Jiahui Shan Jingwei Li Guanglu Shi Yu Huang Li Feng Jianchao Xue Qiao Li Dechao Song Ying Li 《Research in Astronomy and Astrophysics》 2025年第2期36-45,共10页
The in-flight calibration and performance of the Solar Disk Imager(SDI),which is a pivotal instrument of the LyαSolar Telescope onboard the Advanced Space-based Solar Observatory mission,suggested a much lower spatia... The in-flight calibration and performance of the Solar Disk Imager(SDI),which is a pivotal instrument of the LyαSolar Telescope onboard the Advanced Space-based Solar Observatory mission,suggested a much lower spatial resolution than expected.In this paper,we developed the SDI point-spread function(PSF)and Image Bivariate Optimization Algorithm(SPIBOA)to improve the quality of SDI images.The bivariate optimization method smartly combines deep learning with optical system modeling.Despite the lack of information about the real image taken by SDI and the optical system function,this algorithm effectively estimates the PSF of the SDI imaging system directly from a large sample of observational data.We use the estimated PSF to conduct deconvolution correction to observed SDI images,and the resulting images show that the spatial resolution after correction has increased by a factor of more than three with respect to the observed ones.Meanwhile,our method also significantly reduces the inherent noise in the observed SDI images.The SPIBOA has now been successfully integrated into the routine SDI data processing,providing important support for the scientific studies based on the data.The development and application of SPIBOA also paves new ways to identify astronomical telescope systems and enhance observational image quality.Some essential factors and precautions in applying the SPIBOA method are also discussed. 展开更多
关键词 techniques:image processing Sun:chromosphere Sun:flares methods:numerical
在线阅读 下载PDF
Sympathetic Solar Eruption on 2024 February 9 被引量:1
7
作者 Shu-Yue Li Qing-Min Zhang +4 位作者 Bei-Li Ying Li Feng Ying-Na Su Mu-Sheng Lin Yan-Jie Zhang 《Research in Astronomy and Astrophysics》 2025年第1期182-191,共10页
In this paper,we perform a follow-up investigation of the solar eruption originating from active region 13575 on 2024 February 9.The primary eruption of a hot channel generates an X3.4 class flare,a full-halo coronal ... In this paper,we perform a follow-up investigation of the solar eruption originating from active region 13575 on 2024 February 9.The primary eruption of a hot channel generates an X3.4 class flare,a full-halo coronal mass ejection(CME),and an extreme-ultraviolet(EUV)wave.Interaction between the wave and a quiescent prominence(QP)leads to a large-amplitude,transverse oscillation of QP.After the transverse oscillation,QP loses equilibrium and rises up.The ascending motion of the prominence is coherently detected and tracked up to∼1.68 R_(⊙)by the Solar UltraViolet Imager onboard the GOES-16 spacecraft and up to∼2.2 R_(⊙)by the Solar Corona Imager(SCI_UV)of the LyαSolar Telescope onboard the ASO-S spacecraft.The velocity increases linearly from 12.3 to 68.5 km s^(−1)at 18:30 UT.The sympathetic eruption of QP drives the second CME with a typical three-part structure.The bright core comes from the eruptive prominence,which could be further observed up to∼3.3 R_(⊙) by the Large Angle Spectroscopic Coronagraph onboard the Solar and Heliospheric Observatory mission.The leading edge of the second CME accelerates continuously from∼120 to∼277 km s^(−1).The EUV wave plays an important role in linking the primary eruption with the sympathetic eruption. 展开更多
关键词 SUN flares-Sun filaments prominences-Sun coronal mass ejections(CMEs)
在线阅读 下载PDF
宫颈癌组织NSUN6、SULF1表达与临床病理特征关系及预后价值 被引量:1
8
作者 张毅 伍紫蕊 +1 位作者 李玉佳 黄官友 《疑难病杂志》 2025年第7期843-847,854,共6页
目的 研究宫颈癌组织NOP2/Sun RNA甲基转移酶家族成员6(NSUN6)、硫酸酯酶1(SULF1)的表达及其与临床病理特征和预后的关系。方法 选取2019年6月—2021年6月贵州医科大学附属医院妇产科收治的宫颈癌患者92例的临床资料。利用实时荧光定量... 目的 研究宫颈癌组织NOP2/Sun RNA甲基转移酶家族成员6(NSUN6)、硫酸酯酶1(SULF1)的表达及其与临床病理特征和预后的关系。方法 选取2019年6月—2021年6月贵州医科大学附属医院妇产科收治的宫颈癌患者92例的临床资料。利用实时荧光定量PCR和免疫组织化学检测癌组织NSUN6、SULF1 mRNA和蛋白表达;应用R语言分析人类基因组图谱(TCGA)数据库中宫颈癌组织和正常宫颈组织中NSUN6、SULF1表达差异;K-M曲线分析NSUN6、SULF1蛋白表达对宫颈癌患者预后的影响;Cox回归模型筛选宫颈癌预后的影响因素。结果 TCGA数据库分析结果显示,宫颈癌组织中NSUN6、SULF1 mRNA表达高于正常宫颈组织(t/P=22.281/<0.001、53.721/<0.001);宫颈癌患者癌组织NSUN6、SULF1 mRNA表达均高于癌旁组织(t/P=37.619/<0.001、36.442/<0.001),宫颈癌组织NSUN6、SULF1蛋白阳性率均高于癌旁组织(χ^(2)/P=85.546/<0.001、86.891/<0.001);宫颈癌组织NSUN6、SULF1蛋白阳性率在FIGO分期ⅠB2~ⅡB期、淋巴结转移均高于ⅠA~ⅠB1期、无淋巴结转移(t/P=7.934/0.005、5.327/0.021、13.512/<0.001、4.564/0.033);NSUN6阳性与阴性、SULF1阳性与阴性3年总生存(OS)率之间比较,差异均无统计学意义(P>0.05),NSUN6阳性、SULF1阳性患者3年无进展生存(PFS)率均低于阴性患者(χ^(2)/P=8.120/0.004、36.442/<0.001);FIGO分期ⅠB2~ⅡB期、淋巴结转移、NSUN6阳性、SULF1阳性是宫颈癌患者预后不良的危险因素[HR(95%CI)=1.402(1.115~1.764)、1.339(1.094~1.639)、1.374(1.082~1.746)、1.278(1.054~1.548)]。结论 宫颈癌组织中NSUN6、SULF1 mRNA和蛋白表达均升高,两者均与不良临床病理特征相关,是评估宫颈癌预后的标志物。 展开更多
关键词 宫颈癌 NOP2/Sun RNA甲基转移酶家族成员6 硫酸酯酶1 预后
暂未订购
视网膜母细胞瘤组织中NSUN2 mRNA,PRMT5 mRNA表达水平与临床病理学特征及预后的相关性研究
9
作者 万宇 邹云春 +3 位作者 王岚 李娜 何艺岚 王淑 《现代检验医学杂志》 2025年第4期61-66,共6页
目的探讨视网膜母细胞瘤(Rb)组织中NOP2/Sun RNA甲基转移酶家族成员2(NSUN2)、蛋白精氨酸甲基转移酶5(PRMT5)表达水平及与Rb患者生存预后的相关性。方法选取2019年2月~2021年2月首都医科大学附属北京安贞医院南充医院诊治的84例Rb患者(R... 目的探讨视网膜母细胞瘤(Rb)组织中NOP2/Sun RNA甲基转移酶家族成员2(NSUN2)、蛋白精氨酸甲基转移酶5(PRMT5)表达水平及与Rb患者生存预后的相关性。方法选取2019年2月~2021年2月首都医科大学附属北京安贞医院南充医院诊治的84例Rb患者(Rb组),以50例正常视网膜组织为对照组。采用实时荧光定量PCR(qRT-PCR)检测视网膜组织中NSUN2 mRNA,PRMT5 mRNA表达水平。Kaplan-Meier曲线分析NSUN2 mRNA,PRMT5 mRNA对Rb患者预后的影响。COX回归分析影响Rb预后的因素。结果Rb癌组织NSUN2 mRNA(3.11±0.42),PRMT5 mRNA(2.84±0.39)表达高于对照组(0.80±0.23,0.76±0.20),差异具有统计学意义(t=35.935,35.033,均P<0.001)。肿瘤直径≥20mm,未分化型及IIRC分期D~E期Rb癌组织NSUN2 mRNA,PRMT5 mRNA表达高于肿瘤直径<20mm,分化型组织及IIRC分期A~C期,差异具有统计学意义(t=18.297,141.770,16.693;18.663,139.144,39.947,均P<0.001)。NSUN2 mRNA高表达组三年无进展生存率低于低表达组(60.00%vs 88.64%),PRMT5 mRNA高表达组三年无进展生存率低于低表达组(56.10%vs 93.02%),差异具有统计学意义(Log rankχ^(2)=13.440,19.501,均P<0.001)。未分化型、IIRC分期D~E期、NSUN2 mRNA高表达、PRMT5 mRNA高表达是影响Rb患者预后的危险因素(Waldχ^(2)=5.923~7.161,均P<0.001)。结论Rb组织中NSUN2 mRNA,PRMT5 mRNA表达升高,与肿瘤最大径、病理分型及IIRC分期有关,可作为评估Rb生存预后的肿瘤标志物。 展开更多
关键词 视网膜母细胞瘤 NOP2/Sun RNA甲基转移酶家族成员2 蛋白精氨酸甲基转移酶5
暂未订购
Probing Solar Polar Regions
10
作者 DENG Yuanyong TIAN Hui +45 位作者 JIANG Jie YANG Shuhong LI Hao CAMERON Robert GIZON Laurent HARRA Louise WIMMER-SCHWEINGRUBER Robert F AUCHÈRE Frédéric BAI Xianyong BELLOT RUBIO Luis CHEN Linjie CHEN Pengfei CHITTA Lakshmi Pradeep DAVIES Jackie FAVATA Fabio FENG Li FENG Xueshang GAN Weiqun HASSLER Don HE Jiansen HOU Junfeng HOU Zhenyong JIN Chunlan LI Wenya LIN Jiaben NANDY Dibyendu PANT Vaibhav ROMOLI Marco SAKAO Taro KRISHNA PRASAD Sayamanthula SHEN Fang SU Yang TORIUMI Shin TRIPATHI Durgesh WANG Linghua WANG Jingjing XIA Lidong XIONG Ming YAN Yihua YANG Liping YANG Shangbin ZHANG Mei ZHOU Guiping ZHU Xiaoshuai WANG Jingxiu WANG Chi 《空间科学学报》 北大核心 2025年第4期913-942,共30页
The magnetic fields and dynamical processes in the solar polar regions play a crucial role in the solar magnetic cycle and in supplying mass and energy to the fast solar wind,ultimately being vital in controlling sola... The magnetic fields and dynamical processes in the solar polar regions play a crucial role in the solar magnetic cycle and in supplying mass and energy to the fast solar wind,ultimately being vital in controlling solar activities and driving space weather.Despite numerous efforts to explore these regions,to date no imaging observations of the Sun's poles have been achieved from vantage points out of the ecliptic plane,leaving their behavior and evolution poorly understood.This observation gap has left three top-level scientific questions unanswered:How does the solar dynamo work and drive the solar magnetic cycle?What drives the fast solar wind?How do space weather processes globally originate from the Sun and propagate throughout the solar system?The Solar Polarorbit Observatory(SPO)mission,a solar polar exploration spacecraft,is proposed to address these three unanswered scientific questions by imaging the Sun's poles from high heliolatitudes.In order to achieve its scientific goals,SPO will carry six remote-sensing and four in-situ instruments to measure the vector magnetic fields and Doppler velocity fields in the photosphere,to observe the Sun in the extreme ultraviolet,X-ray,and radio wavelengths,to image the corona and the heliosphere up to 45 R_(s),and to perform in-situ detection of magnetic fields,and low-and high-energy particles in the solar wind.The SPO mission is capable of providing critical vector magnetic fields and Doppler velocities of the polar regions to advance our understanding of the origin of the solar magnetic cycle,providing unprecedented imaging observations of the solar poles alongside in-situ measurements of charged particles and magnetic fields from high heliolatitudes to unveil the mass and energy supply that drive the fast solar wind,and providing observational constraints for improving our ability to model and predict the three-dimensional(3D)structures and propagation of space weather events. 展开更多
关键词 SUN Space exploration Solar magnetic cycle Solar wind Space weather
在线阅读 下载PDF
RNA甲基转移酶NSUN2通过介导TEAD1 m5C甲基化修饰促进胃癌细胞的糖酵解作用
11
作者 王春云 阳乐斌 唐芬芬 《华中科技大学学报(医学版)》 北大核心 2025年第6期812-820,883,共10页
目的 探讨NOP2/Sun RNA甲基转移酶2(NSUN2)通过介导TEA结构域转录因子1(TEAD1)5-甲基胞嘧啶(m5C)甲基化修饰对胃癌(gastric cancer, GC)细胞糖酵解的影响及其机制。方法 实时荧光定量PCR(qRT-PCR)检测人胃癌(GC)组织中NSUN2和TEAD1 mRN... 目的 探讨NOP2/Sun RNA甲基转移酶2(NSUN2)通过介导TEA结构域转录因子1(TEAD1)5-甲基胞嘧啶(m5C)甲基化修饰对胃癌(gastric cancer, GC)细胞糖酵解的影响及其机制。方法 实时荧光定量PCR(qRT-PCR)检测人胃癌(GC)组织中NSUN2和TEAD1 mRNA表达水平,Pearson相关分析两者相关性。qRT-PCR和蛋白质印迹法(Western blot)检测人正常胃黏膜上皮细胞GES-1及胃癌AGS、HGC-27、SNU-1和MKN-45等细胞系中NSUN2表达水平。将NSUN2干扰慢病毒(sh-NSUN2)及其阴性对照sh-NC、TEAD1过表达质粒(oe-TEAD1)及其阴性对照(Vector)分别或同时转染至HGC-27细胞中,CCK-8检测细胞增殖活性。葡萄糖摄取、乳酸含量以及三磷酸腺苷(ATP)检测试剂盒评估细胞糖酵解。Western blot检测细胞中糖酵解途径相关蛋白(HK2、LDHA、GLUT1)表达水平。qRT-PCR和Western blot检测细胞中TEAD1 mRNA和蛋白表达水平。RNA测序(RNA-seq)和RNA甲基化免疫共沉淀实验筛选NSUN2的5-甲基胞嘧啶(m5C)修饰靶点,同时采用斑点杂交和双荧光素酶报告基因实验验证其调控功能。结果 与癌旁组织比较,GC组织中NSUN2和TEAD1 mRNA表达水平均显著升高(均P<0.05),且两者呈正相关(r=0.3266,P<0.05)。与GES-1细胞系比较,胃癌细胞系(AGS、HGC-27、SNU-1和MKN-45)中NSUN2 mRNA和蛋白表达水平显著升高,其中在HGC-27细胞系中表达最高(均P<0.05)。沉默NSUN2可显著降低HGC-27细胞增殖活性和糖酵解,下调HK2、LDHA和GLUT1蛋白表达水平,同时细胞中TEAD1 mRNA和蛋白表达水平显著降低(均P<0.05)。TEAD1是NSUN2调控的潜在靶点,沉默NSUN2通过m5C修饰的方式降低TEAD1的表达(P<0.05)。然而,过表达TEAD1可明显逆转沉默NSUN2对HGC-27细胞增殖活性和糖酵解的抑制作用。结论 NSUN2通过m5C依赖性的方式增强TEAD1的表达进而促进GC细胞的糖酵解。 展开更多
关键词 NOP2/Sun RNA甲基转移酶2 TEA结构域转录因子1 5-甲基胞嘧啶 甲基化 胃癌 糖酵解
暂未订购
The First Scientific Flight and Observations of the 50 mm Balloon-borne White-light Coronagraph
12
作者 Kaifeng Kang Min Huang +13 位作者 Yang Liu Jun Lin Tengfei Song Xuefei Zhang Dayang Liu Tao Zhang Yan Li Jingxing Wang Mingzhe Sun Mingyu Zhao Guangqian Liu Xianyong Bai Lidong Xia Yu Liu 《Research in Astronomy and Astrophysics》 2025年第12期105-116,共12页
A 50 mm balloon-borne white-light coronagraph(BBWLC)to observe white-light solar corona over the altitude range from 1.08 R_(⊙)to 1.50 R_(⊙)has recently been indigenously developed by Yunnan Observatories in collabo... A 50 mm balloon-borne white-light coronagraph(BBWLC)to observe white-light solar corona over the altitude range from 1.08 R_(⊙)to 1.50 R_(⊙)has recently been indigenously developed by Yunnan Observatories in collaboration with Shandong University(in Weihai)and Changchun Institute of Optics,Fine Mechanics and Physics,which will significantly improve the ability of China to detect and measure the inner corona.On 2022 October 4,its first scientific flight took place at the Dachaidan area in Qinghai province of China.We briefly describe the BBWLC mission including its optical design,mechanical structure,pointing system,the first flight and results associated with the data processing approach.Preliminary analysis of the data shows that BBWLC imaged the K-corona with three streamer structures on the west limb of the Sun.To further confirm the coronal signals obtained by BBWLC,comparisons were made with observations of the K-coronagraph of the High Altitude Observatory and the Atmospheric Imaging Assembly on board the Solar Dynamics Observatory.We conclude that BBWLC eventually observed the white-light corona in its first scientific flight. 展开更多
关键词 Sun:activity Sun:filaments PROMINENCES Sun:coronal mass ejections(CMEs) Sun:flares Sun:magnetic fields
在线阅读 下载PDF
Research Progress in Solar Flare Prediction Methods
13
作者 Ke Han Zhen Liu +3 位作者 Xian-Yi Zhao Yi-Fei Li De-Quan Zheng Jie Wan 《Research in Astronomy and Astrophysics》 2025年第3期277-306,共30页
Solar flares are one of the strongest outbursts of solar activity,posing a serious threat to Earth’s critical infrastructure,such as communications,navigation,power,and aviation.Therefore,it is essential to accuratel... Solar flares are one of the strongest outbursts of solar activity,posing a serious threat to Earth’s critical infrastructure,such as communications,navigation,power,and aviation.Therefore,it is essential to accurately predict solar flares in order to ensure the safety of human activities.Currently,the research focuses on two directions:first,identifying predictors with more physical information and higher prediction accuracy,and second,building flare prediction models that can effectively handle complex observational data.In terms of flare observability and predictability,this paper analyses multiple dimensions of solar flare observability and evaluates the potential of observational parameters in prediction.In flare prediction models,the paper focuses on data-driven models and physical models,with an emphasis on the advantages of deep learning techniques in dealing with complex and high-dimensional data.By reviewing existing traditional machine learning,deep learning,and fusion methods,the key roles of these techniques in improving prediction accuracy and efficiency are revealed.Regarding prevailing challenges,this study discusses the main challenges currently faced in solar flare prediction,such as the complexity of flare samples,the multimodality of observational data,and the interpretability of models.The conclusion summarizes these findings and proposes future research directions and potential technology advancement. 展开更多
关键词 SUN activity-Sun flares-(Sun )sunspots-Sun magnetic fields-magnetohydrodynamics(MHD)
在线阅读 下载PDF
Frequency of Occurrence of Solar Plasma Disturbances in Interplanetary Space
14
作者 Munendra Singh Y.P.Singh 《Research in Astronomy and Astrophysics》 2025年第3期246-252,共7页
Major solar plasma disturbances are subjected to Lomb-Scargle periodogram and wavelet analysis to determine the occurrence frequency.These disruptions include interplanetary coronal mass ejection,sudden storm commence... Major solar plasma disturbances are subjected to Lomb-Scargle periodogram and wavelet analysis to determine the occurrence frequency.These disruptions include interplanetary coronal mass ejection,sudden storm commencement,high-speed streams,corotating interaction regions,interplanetary shocks and Forbush decreases.We included information on all of the aforementioned solar disturbances for the last six solar cycles,from 1965 to 2023,for this study.Our findings reveal some intriguing and noteworthy results that clearly distinguish between even and odd-numbered solar cycles.The study suggests that the Sun behaves differently in odd and even-numbered solar cycles as it comes from the massive solar eruptions.During even-numbered solar cycles,variations with a period of∼44 days are prominently observed in addition to solar rotation(∼27 days)and extended solar(∼36 days)rotation.However,in addition to solar rotation,prolonged solar rotation,and periods of around 44 days,we also detect a number of intermittent changes with nearly comparable amplitude during the oddnumbered solar cycles.The findings also demonstrate that,in contrast to odd-numbered solar cycles,the emissions rate of these disruptions is more distinct and predictable during even-numbered solar cycles. 展开更多
关键词 SUN activity-Sun CORONAL mass ejections(CMEs)-Sun magnetic fields-Sun rotation-(Sun )solar WIND
在线阅读 下载PDF
The Flash Spectrum Analysis during the 2013 Total Solar Eclipse
15
作者 B.Q.Zhu Z.Xu C.Xiang 《Research in Astronomy and Astrophysics》 2025年第8期81-91,共11页
During the total solar eclipse of 2013 November 3, a fiber-based spectrometer captured the flash spectrum within the wavelength range of 5162–5325Å, with the field-of-view positioned approximately 0.04 R⊙above ... During the total solar eclipse of 2013 November 3, a fiber-based spectrometer captured the flash spectrum within the wavelength range of 5162–5325Å, with the field-of-view positioned approximately 0.04 R⊙above the east limb, near the second-contact point. This placement near the innermost corona, together with the nearby Baily's beads, enabled the detection of emission lines from the photosphere, lower chromosphere, and corona in the same spectral frame. The higher field-of-view made the coronal line Fe XIV 5303Åmore visible, while the reduced intensity of photospheric and lower chromospheric light, caused by terrestrial scattering, also influenced the observed spectrum. Meanwhile, we selected 18 flash emission lines to measure the ratio of the relative line height of the flash emission line to the relative line depth of the corresponding solar absorption line. This ratio serves as a diagnostic of the source function, minimizing opacity effects. Comparison of disk and flash spectra shows that this ratio increases for spectral lines at greater formation heights, being strongest for chromospheric lines(particularly Fe II), moderate for photospheric low-FIP lines, and weakest for photospheric neutral lines. This trend suggests a rising source function with formation height, potentially linked to increasing electron temperatures or influenced by factors observed in flash spectra near active regions as reported in earlier studies. 展开更多
关键词 techniques:spectroscopic ECLIPSES Sun:chromosphere Sun:photosphere Sun:general
在线阅读 下载PDF
Observations of Wave Amplitude Modulation in and Around Sunspots by SDO
16
作者 A.Abedini 《Research in Astronomy and Astrophysics》 2025年第12期20-33,共14页
This study analyzes the evolution and dynamics of intensity oscillations in coronal sunspots and their surroundings using multi-wavelength image data from the Atmospheric Imaging Assembly(AIA)and Helioseismic and Magn... This study analyzes the evolution and dynamics of intensity oscillations in coronal sunspots and their surroundings using multi-wavelength image data from the Atmospheric Imaging Assembly(AIA)and Helioseismic and Magnetic Imager(HMI)on board the Solar Dynamics Observatory(SDO).Intensity time series were extracted and analyzed from consecutive macropixels along thin coronal loop paths originating in a quiet sunspot.Fourier and wavelet analyses of corrected intensity time series reveal dominant 3 and 5 minute oscillations.Signals were filtered using the Fourier and inverse transforms to isolate narrow bands around the dominant oscillation periods.Diagrams and time-distance maps of intensity time series were plotted for Fourierfiltered AIA 131A,171A,193A,and 211A channels,along with SDO/HMI magnetograms and dopplergrams at 6173A.The plots clearly show propagating oscillations with amplitude modulation(AM)across all macropixels along selected coronal paths in nearly all AIA and HMI channels.The phase speeds of the filtered oscillations,measured via slope calculations in time-distance maps,indicate that the intensity disturbances are slow magneto-acoustic waves.These results suggest that AM likely arises from the superposition of counterpropagating waves with slightly different frequencies(beta-like phenomena)due to Doppler shifts from background plasma flow along loop paths.Validating this hypothesis could establish AM's significance in solar coronal seismology for determining background plasma flow speed,the source of long-period oscillations,and coronal plasma heating mechanisms. 展开更多
关键词 The Sun Sun:corona (Sun:)sunspots Sun:oscillations
在线阅读 下载PDF
Two-sided-loop Jet Originates from the Filament Internal Reconnection
17
作者 Yunxue Huang Jialin Li +4 位作者 Zhining Qu Ke Yu Hongfei Liang Rui Xue Xinping Zhou 《Research in Astronomy and Astrophysics》 2025年第11期166-173,共8页
Magnetic reconnection driving a two-sided-loop jet is typically associated with interactions between an emerging bipole and the overlying horizontal magnetic field,or between filaments from separate magnetic systems.L... Magnetic reconnection driving a two-sided-loop jet is typically associated with interactions between an emerging bipole and the overlying horizontal magnetic field,or between filaments from separate magnetic systems.Leveraging high temporal and spatial resolution observations from ground-based and space-borne instruments,we have identified a two-sided-loop jet originating from magnetic reconnection between threads within a single filament.Our observations show that as two initially crossing filamentary threads within the filament converge,reconnection takes place at their intersection.In the Doppler images,distinct redshift and blueshift signals are observed at the locations where the filament threads intersected.This process generates a two-sided-loop jet with outflow speeds of 22.2 and 62.5 km s^(-1).Following reconnection,the original crossing threads transform into two parallel threads that subsequently separate at speeds of 2.8 and 8.3kms^(-1).This observation offers a new perspective on the mechanisms responsible for jet formation. 展开更多
关键词 Sun:activity Sun:filaments PROMINENCES Sun:corona
在线阅读 下载PDF
Latitude Distribution and N-S Asymmetry of GLE Event Source Locations during 1942–2024
18
作者 Xi-Wen Zhang Gui-Ming Le +1 位作者 Xu-Dong Zhao Qi Li 《Research in Astronomy and Astrophysics》 2025年第7期167-173,共7页
We examined the heliolatitude distribution and North-South(N-S)asymmetry of Ground Level Enhancement(GLE)event source locations from 1942 to 2024,finding distinct patterns between the periods 1942–1979 and1980–2024.... We examined the heliolatitude distribution and North-South(N-S)asymmetry of Ground Level Enhancement(GLE)event source locations from 1942 to 2024,finding distinct patterns between the periods 1942–1979 and1980–2024.Between 1942 and 1979,33 GLE events were recorded:5 from the southern hemisphere within[S3,S11]and 28 from the northern hemisphere within[N7,N37].The southeast quadrant was devoid of any source locations for GLE events.45.4%of the source locations of the GLE events were within the latitudinal range of[S15,N15].The remaining source locations of the GLE events were distributed at latitudes above 15°in the northern hemisphere.Between 1980 and 2024,43 GLE events were recorded:25 from the southern hemisphere and 18 from the northern hemisphere,with all events above 30°latitude originating from the northern hemisphere.Approximately 44.2%of the source locations of the GLE events were distributed within the latitudinal band[S15,N15].Over the period from 1942 to 2024,44.7%within[S15,N15],63.2%within[S20,N20],80.3%within[S25,N25],88.2%within[S30,N30],and 11.8%at latitudes above 30°.N-S asymmetry was significant at latitudes above 0°,5°,10°,15°,20°and 30°,with northern hemisphere dominance.Moreover,a strong inverse correlation exists between the number of GLE events from the northern and southern hemispheres at latitudes above 0°,5°,10°,15°,20°,25°and 30°. 展开更多
关键词 Sun:fares Sun:coronal mass ejections(CMEs) Sun:particle emission (Sun:)sunspots
在线阅读 下载PDF
The Sun in Winter
19
作者 张红莲 张超 《中学生英语》 2025年第19期6-6,共1页
In winter,the weather is usually cold and everything seems a bit dull.Butthe sun in winter is special.When the sun shines brightly in the clear blue sky,it brings warm(1)to thecold world.The golden sunlight spreads he... In winter,the weather is usually cold and everything seems a bit dull.Butthe sun in winter is special.When the sun shines brightly in the clear blue sky,it brings warm(1)to thecold world.The golden sunlight spreads here and there and it makes the whitesnow shine like diamonds.Although the trees are usually bare in winter,but(2)they look beautiful with the sunlight falling on them. 展开更多
关键词 SKY weather WINTER trees SNOW SUNLIGHT sun WARMTH
在线阅读 下载PDF
上一页 1 2 154 下一页 到第
使用帮助 返回顶部