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miR-516b-5p靶向NSUN2对乳腺癌细胞增殖和侵袭的影响
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作者 程欣然 范亚楠 +1 位作者 刘俊 皮亚平 《河北医学》 2025年第10期1585-1592,共8页
目的:探讨微小非编码RNA-516b-5p(miR-516b-5p)靶向RNA甲基转移酶家族成员2(NSUN2)对乳腺癌(BC)细胞增殖和侵袭的影响。方法:qRT-PCR实验检测BC癌组织、癌旁组织(n=45)及正常乳腺上皮细胞(MCF-10A)、BC细胞(MCF-7、MDA-MB-231、HCC1937)... 目的:探讨微小非编码RNA-516b-5p(miR-516b-5p)靶向RNA甲基转移酶家族成员2(NSUN2)对乳腺癌(BC)细胞增殖和侵袭的影响。方法:qRT-PCR实验检测BC癌组织、癌旁组织(n=45)及正常乳腺上皮细胞(MCF-10A)、BC细胞(MCF-7、MDA-MB-231、HCC1937)中miR-516b-5p、NSUN2 mRNA表达量。将MCF-7细胞分为NC组、miR-NC组、miR-516b-5p mimic组、sh-NC组、sh-NSUN2组、miR-516b-5p mimic+pcDNA-NC组、miR-516b-5p mimic+pcDNA-NSUN2组,通过克隆形成和Transwell侵袭实验分别检测各组MCF-7细胞增殖和侵袭能力;Western blot实验检测各组细胞中NSUN2、细胞周期蛋白1(CCND1)、细胞周期蛋白依赖性激酶1(CDK1)、基质金属蛋白酶2(MMP2)、基质金属蛋白酶9(MMP9)蛋白表达量;构建裸鼠BC移植瘤模型,并分为Model组、miR-516b-5p mimics组、miR-516b-5p mimic+pcDNA-NSUN2组,测量各组肿瘤质量和体积。结果:与癌旁组织相比,癌组织中miR-516b-5p表达量明显下降,NSUN2 mRNA表达量明显上升(P<0.05)。与MCF-10A细胞相比,MCF-7、MDA-MB-231、HCC1937细胞中miR-516b-5p表达量明显下降,NSUN2 mRNA表达量明显上升(P<0.05),其中MCF-7细胞以上指标变化趋势最为明显。与NC组相比,miR-NC组和sh-NC组细胞各指标无明显变化(P>0.05);与miR-NC组相比,miR-516b-5p mimic组细胞集落形成数量、侵袭细胞数减少,NSUN2 mRNA和NSUN2、CCND1、CDK1、MMP2、MMP9蛋白表达量均明显下降,miR-516b-5p表达量明显上升(P<0.05);与sh-NC组相比,sh-NSUN2组各指标变化趋势与miR-516b-5p mimic组一致(P<0.05);而在miR-516b-5p mimic+pcDNA-NSUN2组中各指标变化趋势与miR-516b-5p mimic组相反(P<0.05)。双荧光素酶实验证实miR-516b-5p与NSUN2存在靶向关系(P<0.05)。裸鼠成瘤实验中,与Model组相比,miR-516b-5p mimics组肿瘤组织质量和体积明显缩小(P<0.05);与miR-516b-5p mimics组相比,miR-516b-5p mimic+pcDNA-NSUN2组肿瘤组织质量和体积明显增大(P<0.05)。结论:miR-516b-5p可通过靶向下调NSUN2表达,抑制BC细胞增殖和侵袭。 展开更多
关键词 微小非编码RNA-516b-5p RNA甲基转移酶家族成员2 乳腺癌 增殖 侵袭
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NSUN2通过介导ARMC9的m5C修饰促进胃癌细胞的增殖、转移和侵袭
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作者 李越 陈栋 +4 位作者 王瑾 彭怡 张远琦 杨芬 王学军 《中国药科大学学报》 北大核心 2025年第5期583-591,共9页
为研究NOP2/Sun RNA甲基转移酶2(NOP2/Sun RNA methyltransferase 2,NSUN2)对胃癌恶性进展的影响及其潜在机制,使用TCGA数据库分析发现胃癌组织中NSUN2表达水平显著升高;Western blot发现,与胃黏膜上皮细胞相比,NSUN2在胃癌细胞中表达上... 为研究NOP2/Sun RNA甲基转移酶2(NOP2/Sun RNA methyltransferase 2,NSUN2)对胃癌恶性进展的影响及其潜在机制,使用TCGA数据库分析发现胃癌组织中NSUN2表达水平显著升高;Western blot发现,与胃黏膜上皮细胞相比,NSUN2在胃癌细胞中表达上调;集落形成实验显示,NSUN2过表达细胞中集落形成能力增高;Transwell实验也证实NSUN2高表达时,发生迁移和侵袭的细胞数量显著增多。进一步通过数据库分析推测ARMC可能是NSUN2的下游靶分子,接着通过MeRIP-qPCR揭示NSUN2高表达可以提高ARMC9的m5C修饰水平,并且降低ARMC9 mRNA的降解速率,从而提高其蛋白表达水平。此外,过表达ARMC9可以增强细胞的集落形成能力和迁移侵袭能力。上述结果显示,NSUN2可以通过提高ARMC9 mRNA的m5C修饰水平,提高其稳定性,增强其表达,从而促进胃癌进展,因此NSUN2和ARMC9可以作为胃癌进展的潜在治疗靶标。 展开更多
关键词 5-甲基胞嘧啶 甲基转移酶 NOP2/sun RNA甲基转移酶2 犰狳重复序列9 胃癌 肿瘤标志物
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GENERALIZATION PERFORMANCE OF MULTI-CATEGORY KERNEL MACHINES——In Memory of Professor Sun Yongsheng
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作者 Hong Chen Luoqing Li 《Analysis in Theory and Applications》 2007年第2期188-195,共8页
Support vector machines are originally designed for binary classification. How to effectively extend it for multi-class classification is still an on-going research issue. In this paper, we consider kernel machines wh... Support vector machines are originally designed for binary classification. How to effectively extend it for multi-class classification is still an on-going research issue. In this paper, we consider kernel machines which are natural extensions of multi-category support vector machines originally proposed by Crammer and Singer. Based on the algorithm stability, we obtain the generalization error bounds for the kernel machines proposed in the paper. 展开更多
关键词 Kernel machine uniform stability generalization error
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Complete Convergence for Sung’s Type Weighted Sums of Dependent Random Variables with General Moment Conditions
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作者 Dehua QIU Yanchun YI Pingyan CHEN 《Journal of Mathematical Research with Applications》 CSCD 2019年第4期395-407,共13页
In this paper, the complete convergence theorems for Sung’s type weighted sums of END random variables and PNQD random variables with general moment conditions are obtained. The theorems extend the related known work... In this paper, the complete convergence theorems for Sung’s type weighted sums of END random variables and PNQD random variables with general moment conditions are obtained. The theorems extend the related known works in the literature. 展开更多
关键词 COMPLETE CONVERGENCE general MOMENT condition END RANDOM VARIABLES PNQD RANDOM VARIABLES
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SUN2调控稳定表达绵羊肺腺瘤病毒囊膜蛋白的OAR-L1细胞的恶性转化能力的研究
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作者 鲍安玉 杜晓悦 +3 位作者 陈思旭 段续接 张培 刘淑英 《中国兽医科学》 北大核心 2025年第5期669-677,共9页
为了探究SAD1/UN84结构域蛋白-2(SAD1/UNC84 domain protein-2,SUN2)在绵羊肺腺瘤病(ovine pulmonary adenocarcnoma,OPA)肿瘤细胞恶性转化中的功能,本试验利用实验室保存的JSRV env慢病毒感染绵羊肺成纤维细胞(OAR-L1),建立了OAR-L1-En... 为了探究SAD1/UN84结构域蛋白-2(SAD1/UNC84 domain protein-2,SUN2)在绵羊肺腺瘤病(ovine pulmonary adenocarcnoma,OPA)肿瘤细胞恶性转化中的功能,本试验利用实验室保存的JSRV env慢病毒感染绵羊肺成纤维细胞(OAR-L1),建立了OAR-L1-Env稳转株。通过qPCR和Western-blot检测JSRV Env和SUN2的表达情况,使用CCK-8、划分愈合试验和软琼脂糖集落形成试验检测细胞的恶性转化能力。此外,分别构建SUN2过表达和干扰质粒,并转染OAR-L1-Env稳转株,利用qPCR和Western-blot检测其SUN2的表达情况,通过CCK-8和划分愈合试验检测细胞的增殖和迁移能力。结果显示,OAR-L1-Env稳转株成功建立,且SUN2表达量显著下调(P<0.05),CCK-8、划分愈合试验和软琼脂糖集落形成试验表明OAR-L1-Env稳转株发生恶性转化。OAR-L1-Env稳转株中过表达SUN2可抑制细胞的增殖和迁移能力(P<0.05),而干扰SUN2则促进细胞的增殖和迁移能力(P<0.05)。综上所述,本研究表明SUN2参与调控JSRV Env诱导的细胞恶性转化,提示其可能对OPA肿瘤生成有抑制作用。本研究为进一步探究SUN2在OPA中的细胞增殖等生物学作用的研究提供了一定的参考资料。 展开更多
关键词 稳转株 囊膜蛋白 sun2 增殖 迁移
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Leader of "the Great Wall"──Chairman and General Manager of the Sinopec Great Wall Lubricant Group Co.,Ltd.Sun Yushuang
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《China's Foreign Trade》 2000年第10期14-15,共2页
关键词 Leader of the Great Wall Ltd.sun Yushuang Chairman and general Manager of the Sinopec Great Wall Lubricant Group Co
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视网膜母细胞瘤组织中NSUN2 mRNA,PRMT5 mRNA表达水平与临床病理学特征及预后的相关性研究
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作者 万宇 邹云春 +3 位作者 王岚 李娜 何艺岚 王淑 《现代检验医学杂志》 2025年第4期61-66,共6页
目的探讨视网膜母细胞瘤(Rb)组织中NOP2/Sun RNA甲基转移酶家族成员2(NSUN2)、蛋白精氨酸甲基转移酶5(PRMT5)表达水平及与Rb患者生存预后的相关性。方法选取2019年2月~2021年2月首都医科大学附属北京安贞医院南充医院诊治的84例Rb患者(R... 目的探讨视网膜母细胞瘤(Rb)组织中NOP2/Sun RNA甲基转移酶家族成员2(NSUN2)、蛋白精氨酸甲基转移酶5(PRMT5)表达水平及与Rb患者生存预后的相关性。方法选取2019年2月~2021年2月首都医科大学附属北京安贞医院南充医院诊治的84例Rb患者(Rb组),以50例正常视网膜组织为对照组。采用实时荧光定量PCR(qRT-PCR)检测视网膜组织中NSUN2 mRNA,PRMT5 mRNA表达水平。Kaplan-Meier曲线分析NSUN2 mRNA,PRMT5 mRNA对Rb患者预后的影响。COX回归分析影响Rb预后的因素。结果Rb癌组织NSUN2 mRNA(3.11±0.42),PRMT5 mRNA(2.84±0.39)表达高于对照组(0.80±0.23,0.76±0.20),差异具有统计学意义(t=35.935,35.033,均P<0.001)。肿瘤直径≥20mm,未分化型及IIRC分期D~E期Rb癌组织NSUN2 mRNA,PRMT5 mRNA表达高于肿瘤直径<20mm,分化型组织及IIRC分期A~C期,差异具有统计学意义(t=18.297,141.770,16.693;18.663,139.144,39.947,均P<0.001)。NSUN2 mRNA高表达组三年无进展生存率低于低表达组(60.00%vs 88.64%),PRMT5 mRNA高表达组三年无进展生存率低于低表达组(56.10%vs 93.02%),差异具有统计学意义(Log rankχ^(2)=13.440,19.501,均P<0.001)。未分化型、IIRC分期D~E期、NSUN2 mRNA高表达、PRMT5 mRNA高表达是影响Rb患者预后的危险因素(Waldχ^(2)=5.923~7.161,均P<0.001)。结论Rb组织中NSUN2 mRNA,PRMT5 mRNA表达升高,与肿瘤最大径、病理分型及IIRC分期有关,可作为评估Rb生存预后的肿瘤标志物。 展开更多
关键词 视网膜母细胞瘤 NOP2/sun RNA甲基转移酶家族成员2 蛋白精氨酸甲基转移酶5
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Analgesic effect and safety of dexmedetomidine-assisted intravenous-inhalation combined general anesthesia in laparoscopic minimally invasive inguinal hernia surgery 被引量:1
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作者 Qian-Xing Lou Ke-Ping Xu 《World Journal of Gastrointestinal Surgery》 2025年第3期161-170,共10页
BACKGROUND Currently,very few studies have examined the analgesic effectiveness and safety of dexmedetomidine-assisted intravenous-inhalation combined general anesthesia in laparoscopic minimally invasive surgery for ... BACKGROUND Currently,very few studies have examined the analgesic effectiveness and safety of dexmedetomidine-assisted intravenous-inhalation combined general anesthesia in laparoscopic minimally invasive surgery for inguinal hernia.AIM To investigate the analgesic effect and safety of dexmedetomidine-assisted intravenous-inhalation combined general anesthesia in laparoscopic minimally invasive surgery for inguinal hernia.METHODS In this retrospective study,94 patients scheduled for laparoscopic minimally invasive surgery for inguinal hernia,admitted to Yiwu Central Hospital between May 2022 and May 2023,were divided into a control group(inhalation combined general anesthesia)and a treatment group(dexmedetomidine-assisted intrave-nous-inhalation combined general anesthesia).Perioperative indicators,analgesic effect,preoperative and postoperative 24-hours blood pressure(BP)and heart rate(HR),stress indicators,immune function levels,and adverse reactions were com-pared between the two groups.RESULTS Baseline data,including age,hernia location,place of residence,weight,monthly income,education level,and underlying diseases,were not significantly different between the two groups,indicating comparability(P>0.05).No significant difference was found in operation time and anesthesia time between the two groups(P>0.05).However,the treatment group exhibited a shorter postoperative urinary catheter removal time and hospital stay than the control group(P<0.05).Preoperatively,no significant differences were found in the visual analog scale(VAS)scores between the two groups(P>0.05).However,at 12,18,and 24 hours postoper-atively,the treatment group had significantly lower VAS scores than the control group(P<0.05).Although no significant differences in preoperative hemodynamic indicators were found between the two groups(P>0.05),both groups experienced some extent of changes in postoperative HR,diastolic BP(DBP),and systolic BP(SBP).Nevertheless,the treatment group showed smaller changes in HR,DBP,and SBP than the control group(P<0.05).Preoperative immune function indicators showed no significant differences between the two groups(P>0.05).However,postoperatively,the treatment group demonstrated higher levels of CD3+,CD4+,and CD4+/CD8+and lower levels of CD8+than the control group(P<0.05).The rates of adverse reactions were 6.38%and 23.40%in the treatment and control groups,respectively,revealing a significant difference(χ2=5.371,P=0.020).CONCLUSION Dexmedetomidine-assisted intravenous-inhalation combined general anesthesia can promote early recovery of patients undergoing laparoscopic minimally invasive surgery for inguinal hernia.It ensures stable blood flow,improves postoperative analgesic effects,reduces postoperative pain intensity,alleviates stress response,improves immune function,facilitates anesthesia recovery,and enhances safety. 展开更多
关键词 DEXMEDETOMIDINE Intravenous-inhalation combined general anesthesia Inguinal hernia Laparoscopic minimally invasive surgery Analgesia SAFETY
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RNA甲基转移酶NSUN2通过介导TEAD1 m5C甲基化修饰促进胃癌细胞的糖酵解作用
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作者 王春云 阳乐斌 唐芬芬 《华中科技大学学报(医学版)》 北大核心 2025年第6期812-820,883,共10页
目的 探讨NOP2/Sun RNA甲基转移酶2(NSUN2)通过介导TEA结构域转录因子1(TEAD1)5-甲基胞嘧啶(m5C)甲基化修饰对胃癌(gastric cancer, GC)细胞糖酵解的影响及其机制。方法 实时荧光定量PCR(qRT-PCR)检测人胃癌(GC)组织中NSUN2和TEAD1 mRN... 目的 探讨NOP2/Sun RNA甲基转移酶2(NSUN2)通过介导TEA结构域转录因子1(TEAD1)5-甲基胞嘧啶(m5C)甲基化修饰对胃癌(gastric cancer, GC)细胞糖酵解的影响及其机制。方法 实时荧光定量PCR(qRT-PCR)检测人胃癌(GC)组织中NSUN2和TEAD1 mRNA表达水平,Pearson相关分析两者相关性。qRT-PCR和蛋白质印迹法(Western blot)检测人正常胃黏膜上皮细胞GES-1及胃癌AGS、HGC-27、SNU-1和MKN-45等细胞系中NSUN2表达水平。将NSUN2干扰慢病毒(sh-NSUN2)及其阴性对照sh-NC、TEAD1过表达质粒(oe-TEAD1)及其阴性对照(Vector)分别或同时转染至HGC-27细胞中,CCK-8检测细胞增殖活性。葡萄糖摄取、乳酸含量以及三磷酸腺苷(ATP)检测试剂盒评估细胞糖酵解。Western blot检测细胞中糖酵解途径相关蛋白(HK2、LDHA、GLUT1)表达水平。qRT-PCR和Western blot检测细胞中TEAD1 mRNA和蛋白表达水平。RNA测序(RNA-seq)和RNA甲基化免疫共沉淀实验筛选NSUN2的5-甲基胞嘧啶(m5C)修饰靶点,同时采用斑点杂交和双荧光素酶报告基因实验验证其调控功能。结果 与癌旁组织比较,GC组织中NSUN2和TEAD1 mRNA表达水平均显著升高(均P<0.05),且两者呈正相关(r=0.3266,P<0.05)。与GES-1细胞系比较,胃癌细胞系(AGS、HGC-27、SNU-1和MKN-45)中NSUN2 mRNA和蛋白表达水平显著升高,其中在HGC-27细胞系中表达最高(均P<0.05)。沉默NSUN2可显著降低HGC-27细胞增殖活性和糖酵解,下调HK2、LDHA和GLUT1蛋白表达水平,同时细胞中TEAD1 mRNA和蛋白表达水平显著降低(均P<0.05)。TEAD1是NSUN2调控的潜在靶点,沉默NSUN2通过m5C修饰的方式降低TEAD1的表达(P<0.05)。然而,过表达TEAD1可明显逆转沉默NSUN2对HGC-27细胞增殖活性和糖酵解的抑制作用。结论 NSUN2通过m5C依赖性的方式增强TEAD1的表达进而促进GC细胞的糖酵解。 展开更多
关键词 NOP2/sun RNA甲基转移酶2 TEA结构域转录因子1 5-甲基胞嘧啶 甲基化 胃癌 糖酵解
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Optimizing CNN Architectures for Face Liveness Detection:Performance,Efficiency,and Generalization across Datasets 被引量:1
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作者 Smita Khairnar Shilpa Gite +2 位作者 Biswajeet Pradhan Sudeep D.Thepade Abdullah Alamri 《Computer Modeling in Engineering & Sciences》 2025年第6期3677-3707,共31页
Face liveness detection is essential for securing biometric authentication systems against spoofing attacks,including printed photos,replay videos,and 3D masks.This study systematically evaluates pre-trained CNN model... Face liveness detection is essential for securing biometric authentication systems against spoofing attacks,including printed photos,replay videos,and 3D masks.This study systematically evaluates pre-trained CNN models—DenseNet201,VGG16,InceptionV3,ResNet50,VGG19,MobileNetV2,Xception,and InceptionResNetV2—leveraging transfer learning and fine-tuning to enhance liveness detection performance.The models were trained and tested on NUAA and Replay-Attack datasets,with cross-dataset generalization validated on SiW-MV2 to assess real-world adaptability.Performance was evaluated using accuracy,precision,recall,FAR,FRR,HTER,and specialized spoof detection metrics(APCER,NPCER,ACER).Fine-tuning significantly improved detection accuracy,with DenseNet201 achieving the highest performance(98.5%on NUAA,97.71%on Replay-Attack),while MobileNetV2 proved the most efficient model for real-time applications(latency:15 ms,memory usage:45 MB,energy consumption:30 mJ).A statistical significance analysis(paired t-tests,confidence intervals)validated these improvements.Cross-dataset experiments identified DenseNet201 and MobileNetV2 as the most generalizable architectures,with DenseNet201 achieving 86.4%accuracy on Replay-Attack when trained on NUAA,demonstrating robust feature extraction and adaptability.In contrast,ResNet50 showed lower generalization capabilities,struggling with dataset variability and complex spoofing attacks.These findings suggest that MobileNetV2 is well-suited for low-power applications,while DenseNet201 is ideal for high-security environments requiring superior accuracy.This research provides a framework for improving real-time face liveness detection,enhancing biometric security,and guiding future advancements in AI-driven anti-spoofing techniques. 展开更多
关键词 Face liveness detection cross-dataset generalization real-time face authentication transfer learning DenseNet201 VGG16 InceptionV3 deep learning
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Dual encoding feature filtering generalized attention UNET for retinal vessel segmentation
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作者 ISLAM Md Tauhidul WU Da-Wen +6 位作者 TANG Qing-Qing ZHAO Kai-Yang YIN Teng LI Yan-Fei SHANG Wen-Yi LIU Jing-Yu ZHANG Hai-Xian 《四川大学学报(自然科学版)》 北大核心 2025年第1期79-95,共17页
Retinal blood vessel segmentation is crucial for diagnosing ocular and cardiovascular diseases.Although the introduction of U-Net in 2015 by Olaf Ronneberger significantly advanced this field,yet issues like limited t... Retinal blood vessel segmentation is crucial for diagnosing ocular and cardiovascular diseases.Although the introduction of U-Net in 2015 by Olaf Ronneberger significantly advanced this field,yet issues like limited training data,imbalance data distribution,and inadequate feature extraction persist,hindering both the segmentation performance and optimal model generalization.Addressing these critical issues,the DEFFA-Unet is proposed featuring an additional encoder to process domain-invariant pre-processed inputs,thereby improving both richer feature encoding and enhanced model generalization.A feature filtering fusion module is developed to ensure the precise feature filtering and robust hybrid feature fusion.In response to the task-specific need for higher precision where false positives are very costly,traditional skip connections are replaced with the attention-guided feature reconstructing fusion module.Additionally,innovative data augmentation and balancing methods are proposed to counter data scarcity and distribution imbalance,further boosting the robustness and generalization of the model.With a comprehensive suite of evaluation metrics,extensive validations on four benchmark datasets(DRIVE,CHASEDB1,STARE,and HRF)and an SLO dataset(IOSTAR),demonstrate the proposed method’s superiority over both baseline and state-of-the-art models.Particularly the proposed method significantly outperforms the compared methods in cross-validation model generalization. 展开更多
关键词 Vessel segmentation Data balancing Data augmentation Dual encoder Attention Mechanism Model generalization
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Observations of Wave Amplitude Modulation in and Around Sunspots by SDO
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作者 A.Abedini 《Research in Astronomy and Astrophysics》 2025年第12期20-33,共14页
This study analyzes the evolution and dynamics of intensity oscillations in coronal sunspots and their surroundings using multi-wavelength image data from the Atmospheric Imaging Assembly(AIA)and Helioseismic and Magn... This study analyzes the evolution and dynamics of intensity oscillations in coronal sunspots and their surroundings using multi-wavelength image data from the Atmospheric Imaging Assembly(AIA)and Helioseismic and Magnetic Imager(HMI)on board the Solar Dynamics Observatory(SDO).Intensity time series were extracted and analyzed from consecutive macropixels along thin coronal loop paths originating in a quiet sunspot.Fourier and wavelet analyses of corrected intensity time series reveal dominant 3 and 5 minute oscillations.Signals were filtered using the Fourier and inverse transforms to isolate narrow bands around the dominant oscillation periods.Diagrams and time-distance maps of intensity time series were plotted for Fourierfiltered AIA 131A,171A,193A,and 211A channels,along with SDO/HMI magnetograms and dopplergrams at 6173A.The plots clearly show propagating oscillations with amplitude modulation(AM)across all macropixels along selected coronal paths in nearly all AIA and HMI channels.The phase speeds of the filtered oscillations,measured via slope calculations in time-distance maps,indicate that the intensity disturbances are slow magneto-acoustic waves.These results suggest that AM likely arises from the superposition of counterpropagating waves with slightly different frequencies(beta-like phenomena)due to Doppler shifts from background plasma flow along loop paths.Validating this hypothesis could establish AM's significance in solar coronal seismology for determining background plasma flow speed,the source of long-period oscillations,and coronal plasma heating mechanisms. 展开更多
关键词 The sun sun:corona (sun:)sunspots sun:oscillations
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宫颈癌组织NSUN6、SULF1表达与临床病理特征关系及预后价值
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作者 张毅 伍紫蕊 +1 位作者 李玉佳 黄官友 《疑难病杂志》 2025年第7期843-847,854,共6页
目的 研究宫颈癌组织NOP2/Sun RNA甲基转移酶家族成员6(NSUN6)、硫酸酯酶1(SULF1)的表达及其与临床病理特征和预后的关系。方法 选取2019年6月—2021年6月贵州医科大学附属医院妇产科收治的宫颈癌患者92例的临床资料。利用实时荧光定量... 目的 研究宫颈癌组织NOP2/Sun RNA甲基转移酶家族成员6(NSUN6)、硫酸酯酶1(SULF1)的表达及其与临床病理特征和预后的关系。方法 选取2019年6月—2021年6月贵州医科大学附属医院妇产科收治的宫颈癌患者92例的临床资料。利用实时荧光定量PCR和免疫组织化学检测癌组织NSUN6、SULF1 mRNA和蛋白表达;应用R语言分析人类基因组图谱(TCGA)数据库中宫颈癌组织和正常宫颈组织中NSUN6、SULF1表达差异;K-M曲线分析NSUN6、SULF1蛋白表达对宫颈癌患者预后的影响;Cox回归模型筛选宫颈癌预后的影响因素。结果 TCGA数据库分析结果显示,宫颈癌组织中NSUN6、SULF1 mRNA表达高于正常宫颈组织(t/P=22.281/<0.001、53.721/<0.001);宫颈癌患者癌组织NSUN6、SULF1 mRNA表达均高于癌旁组织(t/P=37.619/<0.001、36.442/<0.001),宫颈癌组织NSUN6、SULF1蛋白阳性率均高于癌旁组织(χ^(2)/P=85.546/<0.001、86.891/<0.001);宫颈癌组织NSUN6、SULF1蛋白阳性率在FIGO分期ⅠB2~ⅡB期、淋巴结转移均高于ⅠA~ⅠB1期、无淋巴结转移(t/P=7.934/0.005、5.327/0.021、13.512/<0.001、4.564/0.033);NSUN6阳性与阴性、SULF1阳性与阴性3年总生存(OS)率之间比较,差异均无统计学意义(P>0.05),NSUN6阳性、SULF1阳性患者3年无进展生存(PFS)率均低于阴性患者(χ^(2)/P=8.120/0.004、36.442/<0.001);FIGO分期ⅠB2~ⅡB期、淋巴结转移、NSUN6阳性、SULF1阳性是宫颈癌患者预后不良的危险因素[HR(95%CI)=1.402(1.115~1.764)、1.339(1.094~1.639)、1.374(1.082~1.746)、1.278(1.054~1.548)]。结论 宫颈癌组织中NSUN6、SULF1 mRNA和蛋白表达均升高,两者均与不良临床病理特征相关,是评估宫颈癌预后的标志物。 展开更多
关键词 宫颈癌 NOP2/sun RNA甲基转移酶家族成员6 硫酸酯酶1 预后
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2024 US General Election and Changes in its Party Politics
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作者 Xie Tao 《Contemporary World》 2025年第1期44-48,共5页
The US 2024 general election ended with the Republican Party winning the presidential, House and Senate elections at the same time. In the presidential election, the Republican Party not only won more popular votes in... The US 2024 general election ended with the Republican Party winning the presidential, House and Senate elections at the same time. In the presidential election, the Republican Party not only won more popular votes in over 90% counties than in the 2020 general election, but also won seven highly contested swing States with greater edges. This also marks the first time since 2004 that the Republican Party has won a relative majority of popular votes in the presidential election. 展开更多
关键词 House elections US general election presidential election general election Senate elections republican party Republican Party popular votes
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The Sun in Winter
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作者 张红莲 张超 《中学生英语》 2025年第19期6-6,共1页
In winter,the weather is usually cold and everything seems a bit dull.Butthe sun in winter is special.When the sun shines brightly in the clear blue sky,it brings warm(1)to thecold world.The golden sunlight spreads he... In winter,the weather is usually cold and everything seems a bit dull.Butthe sun in winter is special.When the sun shines brightly in the clear blue sky,it brings warm(1)to thecold world.The golden sunlight spreads here and there and it makes the whitesnow shine like diamonds.Although the trees are usually bare in winter,but(2)they look beautiful with the sunlight falling on them. 展开更多
关键词 SKY weather WINTER trees SNOW sunLIGHT sun WARMTH
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子宫内膜癌中NLRC5、NSUN2表达及临床预后价值分析
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作者 郭阳 张凤格 +1 位作者 朱丹 倪琴 《疑难病杂志》 2025年第6期693-698,共6页
目的研究子宫内膜癌(EC)中核苷酸结合寡聚化结构域样受体5(NLRC5)、NOP2/Sun RNA甲基转移酶家族成员2(NSUN2)表达及其与临床病理因素的关系及预后意义。方法选取2016年2月—2019年2月北京市顺义妇幼保健院妇科诊治的EC患者136例的癌组... 目的研究子宫内膜癌(EC)中核苷酸结合寡聚化结构域样受体5(NLRC5)、NOP2/Sun RNA甲基转移酶家族成员2(NSUN2)表达及其与临床病理因素的关系及预后意义。方法选取2016年2月—2019年2月北京市顺义妇幼保健院妇科诊治的EC患者136例的癌组织及癌旁组织。分别采用免疫组织化学、qPCR检测EC癌组织和癌旁组织中NLRC5、NSUN2蛋白和mRNA表达;比较不同临床病理参数EC患者NLRC5、NSUN2 mRNA表达差异;受试者工作特征(ROC)曲线分析NLRC5、NSUN2 mRNA表达对EC患者预后的评估价值;Kaplan-Meier曲线和Cox回归分析影响EC患者预后的因素。结果与癌旁组织比较,癌组织中NLRC5 mRNA和蛋白阳性率降低,NSUN2 mRNA和蛋白阳性率升高(NLRC5:t=14.428、χ^(2)=159.835,P均<0.001;NSUN2:t=31.642、χ^(2)=143.753,P均<0.001);在FIGO分期Ⅲ期、淋巴结转移EC患者癌组织NLRC5 mRNA表达较低,NSUN2 mRNA表达较高(NLRC5:t/P=51.911/<0.001、6.777/<0.001;NSUN2:t/P=47.251/<0.001、16.033/<0.001);EC癌组织中NLRC5、NSUN2 mRNA表达对预后评估的最佳截断值分别为2.52、3.83。NLRC5 mRNA高表达组与低表达组5年总生存率分别为91.67%(22/24)、73.21%(82/112),差异有统计学意义(Log-Rankχ^(2)=3.402,P=0.012)。NSUN2 mRNA高表达组与低表达组5年总生存率分别为42.31%(11/26)、84.55%(93/110),差异有统计学意义(Log-Rankχ^(2)=18.910,P<0.001)。FIGO分期Ⅲ期、淋巴结转移、NSUN2 mRNA高是影响EC患者预后的危险因素[HR(95%CI)=1.449(1.148~1.830),1.442(1.124~1.850),1.570(1.145~2.152)],NLRC5 mRNA高是保护因素[HR(95%CI)=0.611(0.462~0.807)]。结论EC中NLRC5表达降低,NSUN2表达升高,与FIGO分期、淋巴结转移相关,是评估EC预后的可能标志物。 展开更多
关键词 子宫内膜癌 核苷酸结合寡聚化结构域样受体5 NOP2/sun RNA甲基转移酶家族成员2 预后
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Exploration and Practice of General Education Courses on Cybersecurity in Higher Education Institutions
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作者 Xuejing Zhang Yueqin Li +1 位作者 Fang Yang Jingjing Wu 《Journal of Contemporary Educational Research》 2025年第11期306-310,共5页
Strengthening cybersecurity education for college students holds significant importance in achieving the strategic goal of building China into a cyber power.This article begins by discussing the significance and neces... Strengthening cybersecurity education for college students holds significant importance in achieving the strategic goal of building China into a cyber power.This article begins by discussing the significance and necessity of implementing cybersecurity education for university students.Drawing on disciplinary characteristics and student learning analysis,it presents a comprehensive construction process and countermeasures for a general cybersecurity education course,covering aspects such as teaching content development,teaching resource creation,and pedagogical approaches.The aim is to provide reference and guidance for other universities in developing general cybersecurity education courses. 展开更多
关键词 CYBERSECURITY general education Security awareness
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A Generalized Satellite Digital Twin System for Satellite Operations
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作者 LIN Jiawei ZHANG Yujie +1 位作者 WU Xiaoming LIU Jianhua 《Aerospace China》 2025年第1期14-23,共10页
Digital twin shows broad application prospects in the aerospace field.This paper introduces a generalized satellite digital twin system in detail.With the innovative design concepts of modularization,generalization an... Digital twin shows broad application prospects in the aerospace field.This paper introduces a generalized satellite digital twin system in detail.With the innovative design concepts of modularization,generalization and modeling,on the one hand,the system has successfully achieved the reuse of software modules among different satellite models;on the other hand,it has achieved the reuse of software modules between the digital twin and the testing system,significantly improving the development efficiency of the digital twin system.The paper elaborates on the technical architecture and application fields of this digital twin system,and further prospects its future development.At the same time,through a real inorbit case,the engineering value of the digital twin system is strongly demonstrated. 展开更多
关键词 satellite operation generalIZATION digital twin
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Stress-Induced Endogenous Cannabinoid Signaling Contributes to Fear Generalization
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作者 Yanan Yue Xia Zhang Yuan Dong 《Neuroscience Bulletin》 2025年第6期1123-1126,共4页
The phenomenon of fear memory generalization can be defined as the expansion of an individual's originally specific fear responses to a similar yet genuinely harmless stimulus or situation subsequent to the occurr... The phenomenon of fear memory generalization can be defined as the expansion of an individual's originally specific fear responses to a similar yet genuinely harmless stimulus or situation subsequent to the occurrence of a traumatic event[1].Fear generalization within the normal range represents an adaptive evolutionary mechanism to facilitate prompt reactions to potential threats and to enhance the likelihood of survival. 展开更多
关键词 STRESS adaptive mechanism originally specific fear responses fear memory generalization endogenous cannabinoid signaling fear generalization adaptive evolutionary mechanism enhance likelihood survival
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StM:a benchmark for evaluating generalization in reinforcement learning
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作者 YUAN Kaizhao ZHANG Rui +5 位作者 PAN Yansong YI Qi PENG Shaohui GUO Jiaming HE Wenkai HU Xing 《High Technology Letters》 2025年第2期118-130,共13页
The challenge of enhancing the generalization capacity of reinforcement learning(RL)agents remains a formidable obstacle.Existing RL methods,despite achieving superhuman performance on certain benchmarks,often struggl... The challenge of enhancing the generalization capacity of reinforcement learning(RL)agents remains a formidable obstacle.Existing RL methods,despite achieving superhuman performance on certain benchmarks,often struggle with this aspect.A potential reason is that the benchmarks used for training and evaluation may not adequately offer a diverse set of transferable tasks.Although recent studies have developed bench-marking environments to address this shortcoming,they typically fall short in providing tasks that both ensure a solid foundation for generalization and exhibit significant variability.To overcome these limitations,this work introduces the concept that‘objects are composed of more fundamental components’in environment design,as implemented in the proposed environment called summon the magic(StM).This environment generates tasks where objects are derived from extensible and shareable basic components,facilitating strategy reuse and enhancing generalization.Furthermore,two new metrics,adaptation sensitivity range(ASR)and parameter correlation coefficient(PCC),are proposed to better capture and evaluate the generalization process of RL agents.Experimental results show that increasing the number of basic components of the object reduces the proximal policy optimization(PPO)agent’s training-testing gap by 60.9%(in episode reward),significantly alleviating overfitting.Additionally,linear variations in other environmental factors,such as the training monster set proportion and the total number of basic components,uniformly decrease the gap by at least 32.1%.These results highlight StM’s effectiveness in benchmarking and probing the generalization capabilities of RL algorithms. 展开更多
关键词 reinforcement learning(RL) generalIZATION BENCHMARK environment
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