Objective:To investigate the antioxidant enicacy of a biologically active dilerpenoid compound sugiol isolated from Metasequoia glyptostroboides(M.glyptostroboides)in various antioxidant models.Methods:An abietane typ...Objective:To investigate the antioxidant enicacy of a biologically active dilerpenoid compound sugiol isolated from Metasequoia glyptostroboides(M.glyptostroboides)in various antioxidant models.Methods:An abietane type diterpenoid sugiol,isolated from ethyl acetate extract of M.glyptostroboides cones,was analyzed for its antioxidant efficacy as reducing power ability and lipid peroxidation inhibition as well as its ability to scavenge free radicals such as 1,1-diphenyl-2-picryl hydrazyl,nitric oxide,superoxide and hydroxyl radicals.Results:The sugiol showed significant and concentration-dependent antioxidant and free radical scavenging activities.Consequently,the sugiol exerted lipid peroxidation inhibitory effect by 76.3%as compared to a-tocopherol(80.13%)and butylaled hydroxyanisole(76.59%).In addition,the sugiol had significant scavenging activities of l,l-diphenyl-2-picryl hydrazyl,nitric oxide,superoxide and hydroxyl free radicals in a concentration-dependent manner by 78.83%,72.42%,72.99%and 85.04%,when compared to the standard compound ascorbic acid(81.69%,74.62%,73.00%and 73.79%)and a-tocopherol/butylated hydroxyanisole(84.09%,78.61%,74.45%and 70.02%),respectively.Conclusions:These findings justify the biological and traditional uses of M.glyptostroboides or its secondary metabolites as confirmed by its promising antioxidant efficacy.展开更多
目的 探讨以生物合成途径结合高分辨液质联用技术,为近亲缘稀少植物星毛冠盖藤自建成分数据库,实现其化学成分的快速发现。方法 以星毛冠盖藤为研究对象,根据京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)...目的 探讨以生物合成途径结合高分辨液质联用技术,为近亲缘稀少植物星毛冠盖藤自建成分数据库,实现其化学成分的快速发现。方法 以星毛冠盖藤为研究对象,根据京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)生物合成基因挖掘、BioNavi-NP生物逆合成预测及同属植物化学成分研究等手段获得星毛冠盖藤中潜在的化学成分,并自建成分数据库。将自建成分数据库与高分辨质谱数据进行比对,统计整理满足质量误差小于6×10^(-6)的化合物,对其进行裂解规律分析及二级碎片离子比对。对鉴定得到的未知化合物采用reaxys网站进行检索。结果 星毛冠盖藤转录组Unigenes次生代谢KEGG通路主要包括苯丙素生物合成、氨酰-tRNA生物合成、N-聚糖生物合成及萜类骨架生物合成等通路;从瑶药星毛冠盖藤中快速鉴定出76个成分,其中包括1个香豆素类新化合物。结论 以生物合成关联性结合LC-MS技术,能快速高效、低成本的对瑶药星毛冠盖藤中潜在的化学成分进行分析鉴定,一定程度上解决近亲缘稀少植物药的化学成分库建库难的问题,为植物药的化学成分快速发现提供一种新思路及方法。展开更多
基金supported by a grant(NRF-2011-0008199)from the Basie Science Research Program through the National Research Foundation of Korea
文摘Objective:To investigate the antioxidant enicacy of a biologically active dilerpenoid compound sugiol isolated from Metasequoia glyptostroboides(M.glyptostroboides)in various antioxidant models.Methods:An abietane type diterpenoid sugiol,isolated from ethyl acetate extract of M.glyptostroboides cones,was analyzed for its antioxidant efficacy as reducing power ability and lipid peroxidation inhibition as well as its ability to scavenge free radicals such as 1,1-diphenyl-2-picryl hydrazyl,nitric oxide,superoxide and hydroxyl radicals.Results:The sugiol showed significant and concentration-dependent antioxidant and free radical scavenging activities.Consequently,the sugiol exerted lipid peroxidation inhibitory effect by 76.3%as compared to a-tocopherol(80.13%)and butylaled hydroxyanisole(76.59%).In addition,the sugiol had significant scavenging activities of l,l-diphenyl-2-picryl hydrazyl,nitric oxide,superoxide and hydroxyl free radicals in a concentration-dependent manner by 78.83%,72.42%,72.99%and 85.04%,when compared to the standard compound ascorbic acid(81.69%,74.62%,73.00%and 73.79%)and a-tocopherol/butylated hydroxyanisole(84.09%,78.61%,74.45%and 70.02%),respectively.Conclusions:These findings justify the biological and traditional uses of M.glyptostroboides or its secondary metabolites as confirmed by its promising antioxidant efficacy.
文摘目的 探讨以生物合成途径结合高分辨液质联用技术,为近亲缘稀少植物星毛冠盖藤自建成分数据库,实现其化学成分的快速发现。方法 以星毛冠盖藤为研究对象,根据京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)生物合成基因挖掘、BioNavi-NP生物逆合成预测及同属植物化学成分研究等手段获得星毛冠盖藤中潜在的化学成分,并自建成分数据库。将自建成分数据库与高分辨质谱数据进行比对,统计整理满足质量误差小于6×10^(-6)的化合物,对其进行裂解规律分析及二级碎片离子比对。对鉴定得到的未知化合物采用reaxys网站进行检索。结果 星毛冠盖藤转录组Unigenes次生代谢KEGG通路主要包括苯丙素生物合成、氨酰-tRNA生物合成、N-聚糖生物合成及萜类骨架生物合成等通路;从瑶药星毛冠盖藤中快速鉴定出76个成分,其中包括1个香豆素类新化合物。结论 以生物合成关联性结合LC-MS技术,能快速高效、低成本的对瑶药星毛冠盖藤中潜在的化学成分进行分析鉴定,一定程度上解决近亲缘稀少植物药的化学成分库建库难的问题,为植物药的化学成分快速发现提供一种新思路及方法。
