Stutzerimonas have been extensively studied due to their remarkable metabolic and physiological diversity.However,research on its phages is currently limited.In this study,we isolated a novel double-stranded DNA(dsDNA...Stutzerimonas have been extensively studied due to their remarkable metabolic and physiological diversity.However,research on its phages is currently limited.In this study,we isolated a novel double-stranded DNA(dsDNA)phage,vB_SstM-PG1,from the marine environment that infects Stutzerimonas stutzeri G1.Its dsDNA genome is 37204 bp long with a G/C content of 64.14%and encodes 54 open reading frames.The phage possesses a tail packaging structure that is different from known Stutzerimonas stutzeri phages and exhibits structural protein characteristics similar to those of temperate phages.In addition,two genes of toxin-antitoxin system,including YdaS_antitoxin and HEPN_SAV_6107,were found in the vB_SstM-PG1 genome and play important roles in regulating host growth and metabolism.With phylogenetic tree and comparative genomic analysis,it has been determined that vB_SstM-PG1 is not closely related to any phages previously identified in the GenBank database.Instead,it has a connection with enigmatic,uncultured viruses.Specifically,the vB_SstM-PG1 virus exhibits an average nucleotide identity of over 70%with six uncultivated viruses identified in the IMG/VR v4 database.This significant finding has resulted in the identification of a novel viral genus known as Metabovirus.展开更多
Neonicotinoids(NEOs),a fourth-generation pesticide group,are extensively utilized for crop protection in agriculture.However,their excessive use can negatively impact the environment and human health.Biodegradation of...Neonicotinoids(NEOs),a fourth-generation pesticide group,are extensively utilized for crop protection in agriculture.However,their excessive use can negatively impact the environment and human health.Biodegradation offers an eco-friendly method for eliminating these insecticides.This study employed bacterial strains Stutzerimonas sp.SA1 and Pseudomonas sp.SA3,isolated from pesticide-contaminated agricultural soil,to degrade the various NEOs including acetamiprid(AC),imidacloprid(IM),thiamethoxam(TH),and clothianidin(CL).Numerous intermediate compounds were detected during biodegradation,and potential pathways were discussed.The highest reduction of chemical oxygen demand of clothianidin(87%)and acetamiprid(72%)were observed in Stutzerimonas sp.SA1 and Pseudomonas sp.SA3 were AC 72%and CL 66%respectively.A bacterial consortium effectively breakdown the NEOs through the secretion of nitrile hydratase(NH)enzyme.Fourier-transform infrared spectroscopy results confirmed the presence of functional groups alkane and carbonyl in the NEOs residue.The Gas Chromatography Mass Spectrometry data revealed that the Stutzerimonas sp.SA1 has the highest potential of biodegradation efficiency(BE)AC(89%)and IM(87%)than the other NEOs respectively.The Pseudomonas sp.SA3 shoved the higher BE in the AC(90%),IM(97%)and TH(85%)than the bacterium Stutzerimonas sp.SA1.The results suggest that these bacteria produce the degradative enzymes effectively and utilize the NEOs as a carbon source.The results suggest that these bacteria produce the NH degradative enzymes and utilize the NEOs as a carbon source.In conclusion,the strains Stutzerimonas sp.SA1 and Pseudomonas sp.SA3 were identified as potential candidate for the biomineralization and removal of NEOs contaminated environment.展开更多
基金supported by the National Natural Science Foundation of China (Nos.42188102,42120104006,41976117,42176111 and 42306111)the Fundamental Research Funds for the Central Universities (No.201812002 and Andrew McMinn)。
文摘Stutzerimonas have been extensively studied due to their remarkable metabolic and physiological diversity.However,research on its phages is currently limited.In this study,we isolated a novel double-stranded DNA(dsDNA)phage,vB_SstM-PG1,from the marine environment that infects Stutzerimonas stutzeri G1.Its dsDNA genome is 37204 bp long with a G/C content of 64.14%and encodes 54 open reading frames.The phage possesses a tail packaging structure that is different from known Stutzerimonas stutzeri phages and exhibits structural protein characteristics similar to those of temperate phages.In addition,two genes of toxin-antitoxin system,including YdaS_antitoxin and HEPN_SAV_6107,were found in the vB_SstM-PG1 genome and play important roles in regulating host growth and metabolism.With phylogenetic tree and comparative genomic analysis,it has been determined that vB_SstM-PG1 is not closely related to any phages previously identified in the GenBank database.Instead,it has a connection with enigmatic,uncultured viruses.Specifically,the vB_SstM-PG1 virus exhibits an average nucleotide identity of over 70%with six uncultivated viruses identified in the IMG/VR v4 database.This significant finding has resulted in the identification of a novel viral genus known as Metabovirus.
基金supported by the Ongoing Research Funding program(No.ORF-2025-398),King Saud University,Riyadh,Saudi Arabia.
文摘Neonicotinoids(NEOs),a fourth-generation pesticide group,are extensively utilized for crop protection in agriculture.However,their excessive use can negatively impact the environment and human health.Biodegradation offers an eco-friendly method for eliminating these insecticides.This study employed bacterial strains Stutzerimonas sp.SA1 and Pseudomonas sp.SA3,isolated from pesticide-contaminated agricultural soil,to degrade the various NEOs including acetamiprid(AC),imidacloprid(IM),thiamethoxam(TH),and clothianidin(CL).Numerous intermediate compounds were detected during biodegradation,and potential pathways were discussed.The highest reduction of chemical oxygen demand of clothianidin(87%)and acetamiprid(72%)were observed in Stutzerimonas sp.SA1 and Pseudomonas sp.SA3 were AC 72%and CL 66%respectively.A bacterial consortium effectively breakdown the NEOs through the secretion of nitrile hydratase(NH)enzyme.Fourier-transform infrared spectroscopy results confirmed the presence of functional groups alkane and carbonyl in the NEOs residue.The Gas Chromatography Mass Spectrometry data revealed that the Stutzerimonas sp.SA1 has the highest potential of biodegradation efficiency(BE)AC(89%)and IM(87%)than the other NEOs respectively.The Pseudomonas sp.SA3 shoved the higher BE in the AC(90%),IM(97%)and TH(85%)than the bacterium Stutzerimonas sp.SA1.The results suggest that these bacteria produce the degradative enzymes effectively and utilize the NEOs as a carbon source.The results suggest that these bacteria produce the NH degradative enzymes and utilize the NEOs as a carbon source.In conclusion,the strains Stutzerimonas sp.SA1 and Pseudomonas sp.SA3 were identified as potential candidate for the biomineralization and removal of NEOs contaminated environment.
