[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose de...[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil,which were analyzed by 16rDNA sequence analysis.[Result]A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening,a fungus with higher filter paper enzyme activity,and a bacterium（NO.5 strain） with higher of both filter paper enzyme activity and CMC enzyme activity were obtained.The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis.[Conclusion]NO.5 strain was identified as Bacillus subtilis.展开更多
基金Supported by Natural Foundation of Education Department of Henan Province (2010B530001) Science and Technology Innovation Supporting Program Talents of University in Henan Province(2010HASTIT019)+1 种基金Key Technology Research and Demonstration of Large-scale Straw Gasification Equipment (092102310044)Key Technology Research and Demonstration of Large Cascade Straw Digestion Biogas Generating Installation (2009A180013)~~
文摘[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil,which were analyzed by 16rDNA sequence analysis.[Result]A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening,a fungus with higher filter paper enzyme activity,and a bacterium（NO.5 strain） with higher of both filter paper enzyme activity and CMC enzyme activity were obtained.The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis.[Conclusion]NO.5 strain was identified as Bacillus subtilis.
