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高压冷冻-冷冻替代(HPF-FS)技术优化肾脏足细胞和内皮细胞糖萼超微结构的保存
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作者 聂琳 刘晓静 +4 位作者 徐进 黄晨诗 刘轶群 王素霞 李文歌 《电子显微学报》 北大核心 2026年第1期81-88,共8页
肾小球滤过屏障的足细胞与内皮细胞糖萼是维持电荷选择性与机械稳定性的关键结构,其表达异常与足细胞损伤及蛋白尿的发生密切相关。传统透射电镜制样技术因化学交联速率慢、脱水剂诱导收缩等问题,导致糖萼原位结构严重破坏。本研究采用... 肾小球滤过屏障的足细胞与内皮细胞糖萼是维持电荷选择性与机械稳定性的关键结构,其表达异常与足细胞损伤及蛋白尿的发生密切相关。传统透射电镜制样技术因化学交联速率慢、脱水剂诱导收缩等问题,导致糖萼原位结构严重破坏。本研究采用高压冷冻-冷冻替代技术(high pressure freezing-freeze substitution,HPF-FS),通过物理冷冻实现样本毫秒级玻璃态固定,并结合低温脱水,最大程度规避化学试剂的交联损伤与抽提效应。结果表明,HPF-FS技术能显著改善糖萼的形态保存效果,透射电镜下可见足突表面糖萼呈现连续、致密的绒毛状结构,肾小球内皮细胞表面糖萼呈细纤维丝状向管腔延伸,其厚度与空间排布都更接近生理状态。 展开更多
关键词 肾小球 糖萼 高压冷冻-冷冻替代 透射电子显微镜
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SWOT observation revealed internal solitary wave characteristic variations in the Lombok Strait
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作者 Xiaochen WANG Jing WANG +1 位作者 Xudong ZHANG Jiaxin LIU 《Journal of Oceanology and Limnology》 2026年第1期71-84,共14页
Internal solitary waves(ISWs)are an essential dynamic process in the ocean due to their large amplitude and long propagation distance.Traditional satellite observations provide only twodimensional observations of ocea... Internal solitary waves(ISWs)are an essential dynamic process in the ocean due to their large amplitude and long propagation distance.Traditional satellite observations provide only twodimensional observations of ocean signatures induced by ISWs.The Surface Water and Ocean Topography(SWOT)satellite has drawn significant attention due to its high resolution and threedimensional observation capabilities.SWOT can generate high-precision three-dimensional sea surface topography,capture sea surface undulations,and reveal ISW-related surface oscillations,thus offering a new perspective for studying ISWs.We collected 43 SWOT observations with clear ISW signatures in the Lombok Strait from August 2023 to June 2024.Based on collected data,the ISW imaging characteristics and distributions were analyzed,and the ISW-related sea level anomaly(SLA)data were measured by the SWOT to calculate the ISW amplitude and reveal the amplitude variations during the propagation along the wave crest.The ISW amplitudes generally range between 10 and 100 m,with most ISW amplitudes between 20 and 40 m.By analyzing two consecutive generated ISW packets,we identified the spreading effect along ISW wave crests,which manifests as ISW amplitude decrease with increase in propagation distance,and the amplitude distribution is non-uniform along the wave crest.Further analysis of the propagation paths of the maximum amplitude of ISW moving northward through the Lombok Strait revealed that these maxima are predominantly oriented in northeast direction.Finally,the relationship between the amplitude of ISW and the resulting SLA was analyzed.The Pearson correlation coefficient between these two variables is as high as 0.90,which suggests a strong positive correlation between amplitude and SLA.Furthermore,this relationship is closely related to the water depth,indicating that the three-dimensional sea surface observations provided by SWOT offer crucial observational data for the inversion of amplitudes of ISW. 展开更多
关键词 internal solitary wave(ISW) Surface Water and Ocean Topography(SWOT) AMPLITUDE Lombok strait
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FS-LASIK与SMILE对近视患者术后早期角膜生物力学影响的差异
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作者 包刀知漫 燕振国 《国际眼科杂志》 2026年第2期221-227,共7页
目的:应用眼反应分析仪(ORA)观察不同近视程度患者行飞秒激光辅助的准分子激光原位角膜磨镶术(FS-LASIK)或飞秒激光小切口微透镜取出术(SMILE)术前术后不同时间角膜生物力学的变化情况,探讨两种手术方式及近视程度对角膜生物力学的影响... 目的:应用眼反应分析仪(ORA)观察不同近视程度患者行飞秒激光辅助的准分子激光原位角膜磨镶术(FS-LASIK)或飞秒激光小切口微透镜取出术(SMILE)术前术后不同时间角膜生物力学的变化情况,探讨两种手术方式及近视程度对角膜生物力学的影响是否存在差异。方法:病例系列研究。选取2023年12月至2024年6月兰州华厦眼科医院接受FS-LASIK或SMILE手术的近视患者共132眼。根据术式不同分为FS-LASIK组和SMILE组,再根据等效球镜度数(SE)分为高度近视组(-10.00 D<SE≤-6.00 D)和低中度近视组(-6.00 D<SE≤-0.50 D)。比较不同分组间患者术后不同时间的裸眼视力(UCVA)、最佳矫正视力(BCVA)、SE、中央角膜厚度(CCT)、角膜补偿眼压(IOPcc)、角膜滞后量(CH)和角膜阻力因子(CRF)等参数变化情况。结果:FS-LASIK组和SMILE组角膜生物力学状态具有良好的可比性。术后3 mo,FS-LASIK组与SMILE组患者SE较术前显著升高,UCVA值、CCT、IOPcc较术前显著降低(均P<0.05),但两组间上述指标比较无差异(均P>0.05)。术后1 d,FS-LASIK组与SMILE组患者CH和CRF均显著下降(均P<0.05),与SMILE组相比,FS-LASIK下降更显著(P<0.05);术后1、3 mo,两组患者CH和CRF均较术后早期有所回升并趋于稳定,但仍低于术前(均P<0.05),且FS-LASIK组低于SMILE组(均P<0.05)。SMILE组内,高度近视组与中低度近视组相比,术后1 d,1 wk时的CH和CRF下降更显著(均P<0.05)。结论:FS-LASIK与SMILE手术术后具有较好的安全性、可预测性和有效性,但均会导致角膜生物力学降低,且FS-LASIK降低更显著。术后早期高度近视组患者角膜生物力学降低更显著,而术后3 mo,近视程度对角膜生物力学的影响无差异。 展开更多
关键词 激光 飞秒激光辅助的准分子激光原位角膜磨镶术(fs-LASIK) 飞秒激光小切口微透镜取出术(SMILE) 角膜生物力学 眼反应分析仪
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Formation and evolution of the tertiary carbonate reefs in the Madura Strait Basin of Indonesia 被引量:4
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作者 RAN Weimin LUAN Xiwu +5 位作者 LU Yintao LIU Hong YANG Jiajia ZHAO Yang HE Wenchang YAN Zhonghui 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2019年第1期47-61,共15页
Analysis of 2 D seismic data over 4 500 km in length from the Madura Strait Basin in the East Java Sea reveals seismic re?ection characteristics of reefs and associated sedimentary bodies, including asymmetrical or sy... Analysis of 2 D seismic data over 4 500 km in length from the Madura Strait Basin in the East Java Sea reveals seismic re?ection characteristics of reefs and associated sedimentary bodies, including asymmetrical or symmetrical dome re?ections, slope progradational re?ections, chaotic re?ections and discontinuous strong re?ections inside the reef, which onlap the ?ank of the reef. It is concluded that the developmental paleo-environment of most reefs is mainly conducive to shallow marine carbonate platform facies and platform margin facies, based on well core data, variations in seismic facies and strata thickness.The formation and evolution of all reefs are primarily in?uenced by the tectonic framework of the Madura Strait Basin. Platform margin reefs are principally controlled by two types of structures: one is a series of E-W trending Paleogene normal faults, and the other is an E-W trending Neogene inversion structures. In addition, wave actions, tidal currents and other ocean currents play an accelerated role in sorting, rounding and redeposition for the accumulation and evolution of reefs. Tertiary reefs in the MSB can be divided into four types: 1) an open platform coral reef of Late Oligocene to Early Miocene, 2) a platform margin coral reef controlled by normal faults in Late Oligocene to Early Miocene, 3) a platform margin Globigerina moundreef controlled by a "hidden" inversion structure in Early Pliocene, and 4) a platform margin Globigerina mound-reef controlled by thrust faults in the early Pliocene. Patterns of the formation and evolution of reefs are also suggested. 展开更多
关键词 reef seismic reflection characteristics FORMATION and EVOLUTION TERTIARY Madura strait Basin Indonesia
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30%呋虫胺·氯噻啉FS的研究与开发 被引量:1
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作者 张大卫 樊梅云 任新峰 《世界农药》 2025年第1期49-54,共6页
采用湿法砂磨,研制30%呋虫胺·氯噻啉种子处理悬浮剂(FS)。通过助剂筛选,获得30%呋虫胺·氯噻啉FS的优选配方。其优选配方:呋虫胺10%,氯噻啉20%,TSC-3004%,TSC-4303%,丙三醇5%,硅酸镁铝0.5%,白炭黑0.5%,黄原胶0.05%,ST42.5%,AS3... 采用湿法砂磨,研制30%呋虫胺·氯噻啉种子处理悬浮剂(FS)。通过助剂筛选,获得30%呋虫胺·氯噻啉FS的优选配方。其优选配方:呋虫胺10%,氯噻啉20%,TSC-3004%,TSC-4303%,丙三醇5%,硅酸镁铝0.5%,白炭黑0.5%,黄原胶0.05%,ST42.5%,AS3488%,卡松0.2%,SAG 15720.01%,去离子水补足。该配方各项技术指标符合产品质量标准,室内安全性试验表明该制剂对水稻种子安全性高且对生长有促进作用。 展开更多
关键词 呋虫胺 氯噻啉 fs 安全性试验
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黑曲霉FS10脱除喷浆玉米皮中玉米赤霉烯酮的发酵条件优化
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作者 吴梦影 杨阳 +4 位作者 王进瑶 叶永丽 盛利娜 纪剑 孙秀兰 《食品与生物技术学报》 北大核心 2025年第9期40-50,共11页
【目的】确定通过黑曲霉(Aspergillus niger,A.niger)FS10进行固态发酵来脱除喷浆玉米皮中玉米赤霉烯酮(zearalenone,ZEN)的最佳发酵条件。【方法】以ZEN脱除率为指标,通过单因素试验和响应面法,对发酵过程中的主要影响因素(接种体积质... 【目的】确定通过黑曲霉(Aspergillus niger,A.niger)FS10进行固态发酵来脱除喷浆玉米皮中玉米赤霉烯酮(zearalenone,ZEN)的最佳发酵条件。【方法】以ZEN脱除率为指标,通过单因素试验和响应面法,对发酵过程中的主要影响因素(接种体积质量比、发酵温度、发酵时间、料液比)进行优化,并比较了最优发酵条件下,喷浆玉米皮在固态发酵前后的主要营养物质的质量分数变化。【结果】黑曲霉FS10脱除喷浆玉米皮中ZEN的最优发酵条件为接种体积质量比120μL∶1 g、发酵温度26℃、发酵时间5.5 d、料液比1 g∶1.4 mL。在该条件下,黑曲霉菌株对喷浆玉米皮中ZEN的脱除率达(70.04±1.88)%。且相较于发酵前,发酵后的喷浆玉米皮中粗蛋白质质量分数从(19.92±0.20)%提升至(21.94±0.42)%,总氨基酸质量分数从(16.240±0.165)%提升至(18.510±0.034)%。【结论】黑曲霉FS10不仅能高效脱除喷浆玉米皮中的ZEN,还能提升喷浆玉米皮作为动物饲料的营养价值。该研究为受ZEN污染的喷浆玉米皮的合理利用和充分开发提供了理论依据。 展开更多
关键词 喷浆玉米皮 黑曲霉fs10 玉米赤霉烯酮 响应面法
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黑曲霉FS10菌株发酵对玉米胚芽粕中玉米赤霉烯酮脱除及品质影响
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作者 王进瑶 杨阳 +3 位作者 叶永丽 吴梦影 纪剑 孙秀兰 《中国粮油学报》 北大核心 2025年第6期30-37,共8页
为有效脱除玉米胚芽粕中广泛存在的玉米赤霉烯酮(zearalenone,ZEN)毒素并改善产品品质,本研究以黑曲霉菌株FS10为发酵菌脱除玉米胚芽粕中ZEN,考察了FS10孢子接种量、发酵温度、发酵时间、料水比对ZEN脱除率的影响,并分析发酵前后产品风... 为有效脱除玉米胚芽粕中广泛存在的玉米赤霉烯酮(zearalenone,ZEN)毒素并改善产品品质,本研究以黑曲霉菌株FS10为发酵菌脱除玉米胚芽粕中ZEN,考察了FS10孢子接种量、发酵温度、发酵时间、料水比对ZEN脱除率的影响,并分析发酵前后产品风味和营养成分的变化。结果表明,FS10脱除玉米胚芽粕中ZEN的最佳条件为接种质量分数15%、发酵温度30℃、发酵时间4 d、料水比1∶2 g/mL,脱除率为61.85%。固相微萃取-气相色谱-质谱法分析显示,发酵后玉米胚芽粕中的挥发性物质种类明显增加,包括具有独特香味的3-辛酮、异戊醛等物质。发酵后,玉米胚芽粕总蛋白、粗脂肪和总氨基酸质量分数分别提高了27.99%、8.02%、27.96%,粗纤维质量分数从12.21%降低到9.49%。黑曲霉FS10菌株发酵对玉米胚芽粕中的ZEN具有较高的脱除能力,且可改善胚芽粕风味与营养。 展开更多
关键词 黑曲霉fs10 玉米胚芽粕 玉米赤霉烯酮 脱毒 挥发性化合物
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E2Fs在宫颈癌中的作用及机制研究进展
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作者 马月月 温松泉 赵卫红 《临床肿瘤学杂志》 2025年第2期190-194,共5页
E2Fs是细胞周期的重要调节因子,它们通过控制参与DNA复制和细胞周期进程的众多靶基因的转录来调节细胞周期的各个阶段。E2F家族大致可分为转录激活因子(E2F1、E2F2和E2F3a)和转录抑制因子(E2F3b、E2F4、E2F5、E2F6、E2F7和E2F8)两大类,... E2Fs是细胞周期的重要调节因子,它们通过控制参与DNA复制和细胞周期进程的众多靶基因的转录来调节细胞周期的各个阶段。E2F家族大致可分为转录激活因子(E2F1、E2F2和E2F3a)和转录抑制因子(E2F3b、E2F4、E2F5、E2F6、E2F7和E2F8)两大类,不仅在调控正常细胞周期中发挥重要作用,而且在宫颈癌中也有着不容忽视的影响。E2Fs作为效应分子参与了宫颈癌的发生发展,并且在作为宫颈癌的预后生物标志物和潜在的治疗靶点方面也有着良好的前景。这篇综述总结了近些年来各个E2F家族成员在宫颈癌中的作用及其机制研究进展,以期为宫颈癌的诊治提供新的思路。 展开更多
关键词 子宫颈肿瘤 E2fs 转录因子 机制 研究进展
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SMILE与FS-LASIK术后角膜上皮重塑的临床研究
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作者 张杨婧 马立威 +3 位作者 张帆 柯春梅 王瑞夫 祖丽皮娅 《国际眼科杂志》 CAS 2025年第1期37-41,共5页
目的:比较飞秒激光小切口角膜基质透镜取出术(SMILE)与飞秒激光辅助准分子激光原位角膜磨镶术(FS-LASIK)术后角膜上皮厚度(CET)的变化特点。方法:收集2022-12/2023-11在乌鲁木齐爱尔眼科医院接受屈光手术患者187例187眼。按手术方式分为... 目的:比较飞秒激光小切口角膜基质透镜取出术(SMILE)与飞秒激光辅助准分子激光原位角膜磨镶术(FS-LASIK)术后角膜上皮厚度(CET)的变化特点。方法:收集2022-12/2023-11在乌鲁木齐爱尔眼科医院接受屈光手术患者187例187眼。按手术方式分为SMILE组110例110眼和FS-LASIK组77例77眼。应用眼前节光学相干断层扫描技术(OCT)分别于术前和术后1 wk,1、3、6 mo测量患者的CET。结果:比较术后6 mo时角膜中央区、旁中央区、中周区角膜上皮厚度变化量(△CET),SMILE的特点为中央区增厚最明显,中周区上皮最少;FS-LASIK的特点为旁中央区增厚最明显,中周区最少。术后1 wk,1、3、6 mo时SMILE与FS-LASIK组角膜0-7 mm范围的平均ΔCET与术前等效球镜均具有相关性。结论:SMILE和FS-LASIK术后6 mo内上皮增厚程度随时间变化有相似的趋势和不同的特点,二者△CET均与术前等效球镜正相关。 展开更多
关键词 飞秒激光小切口角膜基质透镜取出术(SMILE) 飞秒激光辅助准分子激光原位角膜磨镶术(fs-LASIK) 角膜上皮重塑 光学相干断层扫描技术(OCT)
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FS-LASIK与SMILE治疗中度近视的疗效比较 被引量:2
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作者 朱丹 周丹 +1 位作者 王青夏 马晓昀 《国际眼科杂志》 2025年第10期1672-1677,共6页
目的:比较全飞秒激光小切口角膜基质内透镜取出术(SMILE)与飞秒激光辅助准分子激光原位角膜磨镶术(FS-LASIK)治疗中度近视的疗效。方法:回顾性研究。选择2022年8月至2024年10月本院收治的中度近视患者100例100眼(均取右眼数据进行研究)... 目的:比较全飞秒激光小切口角膜基质内透镜取出术(SMILE)与飞秒激光辅助准分子激光原位角膜磨镶术(FS-LASIK)治疗中度近视的疗效。方法:回顾性研究。选择2022年8月至2024年10月本院收治的中度近视患者100例100眼(均取右眼数据进行研究)。根据选择的手术方式不同分为FS-LASIK组52例给予FS-LASIK治疗,SMILE组48例给予SMILE治疗。术后随访6 mo,比较两组患者视力恢复情况、手术前后等效球镜度、角膜曲率、角膜Q值、中央角膜厚度、角膜体积、高阶像差、角膜生物力学参数以及并发症发生情况。结果:术后3、6 mo,两组患者裸眼视力(UCVA)和等效球镜度数较术前均改善(均P<0.05)。术后6 mo,两组患者角膜曲率、中央角膜厚度、角膜体积均降低,且FS-LASIK组角膜体积更低;两组Q值均较术前升高,且FS-LASIK组更高(均P<0.001);两组患者术后总高阶像差、球差、三叶草相差均升高,且FS-LASIK组更高(均P<0.001);两组患者综合半径(IR)、反向凹面半径(ICR)、形变幅度比值(DAR2)均升高,硬度参数(SP-A1)、最大压陷曲率半径(HC-Radius)、相关厚度-水平方向(ARTh)均降低(均P<0.001)。两组患者并发症发生率比较无差异(P>0.05)。结论:FS-LASIK与SMILE均有助于改善中度近视患者的视力,术后早期角膜形态变化各具特点,术后接受FS-LASIK的患者角膜Q值、高阶像差较大。 展开更多
关键词 全飞秒激光小切口角膜基质内透镜取出术(SMILE) 飞秒激光辅助准分子激光原位角膜磨镶术(fs-LASIK) 中度近视 疗效
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茅苍术AlCYP71FS9基因的克隆及表达分析
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作者 杨成 陈畅 +4 位作者 高冰彦 张百依 郑鲲鹏 余坤 龚玲 《中药材》 北大核心 2025年第5期1107-1112,共6页
目的:克隆茅苍术AlCYP71FS9基因,了解该基因在苍术品质形成的过程中可能发挥的作用。方法:根据转录组测序信息设计引物,采用聚合酶链式反应(PCR)对茅苍术AlCYP71FS9基因进行扩增及克隆,利用在线软件对AlCYP71FS9基因编码蛋白进行生物信... 目的:克隆茅苍术AlCYP71FS9基因,了解该基因在苍术品质形成的过程中可能发挥的作用。方法:根据转录组测序信息设计引物,采用聚合酶链式反应(PCR)对茅苍术AlCYP71FS9基因进行扩增及克隆,利用在线软件对AlCYP71FS9基因编码蛋白进行生物信息学预测。构建35S启动的PCNG绿色荧光蛋白瞬时表达载体,利用激光共聚焦显微镜对该基因的亚细胞定位进行实测验证。采用实时荧光定量qRT-PCR技术分析该基因在茅苍术不同组织中的相对表达量。结果:茅苍术AlCYP71FS9基因开放阅读框(ORF)的长度为1491 bp,编码497个氨基酸;相对分子质量为56.77 kDa,等电点为7.16,属于亲水性蛋白,且存在P450酶共识别序列FxxGxRxCxG、PERF结构域以及ExxR结构域,属于细胞色素P450超家族成员;AlCYP71FS9基因编码蛋白定位于细胞的内质网;该基因在茅苍术老根茎中的表达量最高,且与茅苍术的茎、叶和嫩根茎均存在显著性差异(P<0.01)。结论:该研究成功克隆到与茅苍术中萜类化合物合成相关的AlCYP71FS9基因,为今后深入开展AlCYP71FS9基因功能及其对茅苍术品质优劣的影响等研究奠定了必要的基础。 展开更多
关键词 茅苍术 AlCYP71fs9基因 生物信息学分析 亚细胞定位
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Geochemical Compositions and Transport Patterns of Surface Sediments from the Malacca Strait
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作者 AI Lina LIU Shengfa +6 位作者 ZHANG Hui CAO Peng LI Xue QI Wenjing WU Kaikai MOHAMED Che Abd Rahim SHI Xuefa 《Journal of Ocean University of China》 2025年第6期1613-1623,共11页
The Malacca Strait(MS)is a vital conduit for the exchange of water and sediment between the Indian Ocean and the Pacific Ocean,serving as a critical‘gateway'for sediment transport.Here,we present the geochemical ... The Malacca Strait(MS)is a vital conduit for the exchange of water and sediment between the Indian Ocean and the Pacific Ocean,serving as a critical‘gateway'for sediment transport.Here,we present the geochemical characteristics of surface sediments in the MS to elucidate the relationship between terrestrial material inputs and modern oceanic dynamic transport processes in the strait.The results reveal that the MS can be divided into three distinct geochemical provinces.ProvinceⅠ,located in the central region of the strait,is characterized by residual deposits.The preservation of these residual deposits can be attributed to the restricted sediment supply and the relatively weaker modern sedimentary hydrodynamic conditions.ProvinceⅡ,situated to the north of ProvinceⅠ,exhibits provenance differences between its southern and northern regions.The northern region is primarily supplied by sediments originating from the eastern shelf of the Andaman Sea,whereas sediments derived from Sumatra and the Sunda Shelf are predominantly deposited in the southern part of ProvinceⅡ.ProvinceⅢextends along the western coast of the Malaysian Peninsula,with sediments primarily sourced from the Malaysian Peninsula and the Sunda Shelf,while contributions from Sumatra and the eastern shelf of the Andaman Sea are negligible.River sediments from the Malaysian Peninsula and Sumatra are transported northwestward along their respective coasts by prevailing currents,which also facilitate the transportation of Sunda Shelf sediments within the strait,while sediments from the eastern shelf of the Andaman Sea are delivered to the MS via southward coastal currents during the southwest monsoon period.The southward currents and well-developed eddies potentially impede the northward transport of sediments from the Sunda Shelf and restrict the distribution of Andaman Sea sediments within the strait.This study substantially enhances the understanding of source-to-sink processes in the Indo-Pacific region. 展开更多
关键词 surface sediment geochemical composition PROVENANCE SEDIMENTATION Malacca strait
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FS2 encodes an ARID-HMG transcription factor that regulates fruit spine density in cucumber
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作者 Hui Du Yue Chen +8 位作者 Liangrong Xiong Juan Liu Keyan Zhang Ming Pan Haifan Wen Huanle He Run Cai Junsong Pan Gang Wang 《Journal of Integrative Agriculture》 2025年第3期1080-1091,共12页
Fruit spine density is an important commercial trait for cucumber(Cucumis sativus L.).Most North China-type cucumbers that are grown over large areas have a dense-spine phenotype,which directly affects the appearance ... Fruit spine density is an important commercial trait for cucumber(Cucumis sativus L.).Most North China-type cucumbers that are grown over large areas have a dense-spine phenotype,which directly affects the appearance quality,storage,and transportation of the fruits.Here,we isolated a novel few spines mutant(fs2)from the wild-type(WT)inbred line WD1,a North China-type cucumber with high density fruit spines,by an ethyl methanesulfonate(EMS)mutagenesis treatment.Genetic analysis revealed that the phenotype of fs2 is controlled by a single recessive nuclear gene.We fine-mapped the fs2 locus using F_(2) and BC_(1) populations(1,802 and 420 individuals,respectively),which showed that the candidate gene of FS2(Csa4G652850)encodes an ARID-HMG transcription factor containing an AT-rich interaction domain(ARID)and a high mobility group box domain(HMG).One SNP(C to T)and one InDel(a 40-bp deletion)in the coding region of FS2 result in amino acid variation and premature translation termination in the fs2 mutant,respectively.FS2 was found to be highly expressed in the apical buds and young ovaries.In addition,experiments suggest that FS2 participates in the regulation of fruit spine initiation by activating the expression of the Tril gene in cucumber.This work provides not only an important reference for understanding the molecular mechanisms of fruit spine development but also an important resource for fruit appearance quality breeding in cucumber. 展开更多
关键词 CUCUMBER few spines fs2 TRICHOME ARID-HMG transcription factor
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The role of Luzon Strait transport in shallow meridional overturning circulation of South China Sea
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作者 Kun Jiang Yu Wang +1 位作者 Yan Sun Jian Lan 《Acta Oceanologica Sinica》 2025年第1期28-35,共8页
The impacts of the Luzon Strait transport on shallow meridional overturning circulation(SMOC)in the South China Sea(SCS)have been pointed out by previous studies,but the issue whether the Luzon Strait transport domina... The impacts of the Luzon Strait transport on shallow meridional overturning circulation(SMOC)in the South China Sea(SCS)have been pointed out by previous studies,but the issue whether the Luzon Strait transport dominates the SMOC formation still remains open.The Helmholtz decomposition is applied based on the ocean general circulation model for the earth simulator products to address this issue.Results show that the motion caused by the Luzon Strait transport is characterized as an obvious southward flow between 13°N and 20°N.After this motion being removed,the clockwise winter SMOC and the anticlockwise summer SMOC can still exist significantly.The SMOC existence and its seasonal variation are also reproduced in the numerical simulation with the Luzon Strait closed.Both results of the Helmholtz decomposition and numerical experiment suggest that the SMOC formation and its seasonal variation are not dominated by the Luzon Strait transport.The SCS monsoon is the primary driving factor for the SMOC,which is related to the physical processes within the SCS. 展开更多
关键词 South China Sea Luzon strait transport shallow meridional overturning circulation Helmholtz decomposition
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Proportion and variability of South China Sea water joining the Indonesian Throughflow in the Makassar Strait
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作者 Shuaichen Xin Mingting Li +1 位作者 Jinchi Xie Dongxiao Wang 《Acta Oceanologica Sinica》 2025年第6期1-14,共14页
The Indonesian Throughflow(ITF),mainly through the Makassar Strait,transports amounts of water and salt from the tropical Pacific Ocean to the Indian Ocean,playing a crucial role in modulating heat and energy budget b... The Indonesian Throughflow(ITF),mainly through the Makassar Strait,transports amounts of water and salt from the tropical Pacific Ocean to the Indian Ocean,playing a crucial role in modulating heat and energy budget between two oceans.The South China Sea Throughflow(SCSTF)significantly contributes to the net transport of the ITF via Karimata Strait and Mindoro-Sibutu Passage.However,the specific proportion and variability of South China Sea(SCS)water joining the ITF are still unclear.Based on high-resolution reanalysis data and a Lagrangian particle tracking method-Connectivity Modelling System(CMS),we quantified the proportion and variability of SCS water joining the ITF in the Makassar Strait.The results show that about 16.41%of the particles released in the Makassar Strait could be back-tracked from the SCS and 42.45%from the western Pacific Ocean.The particles through Mindoro Strait and Karimata Strait are about 10.55%and 3.39%,respectively.About 14.56%and 15.42%particles are trapped in the Sulu and Sulawesi seas.The proportion of SCS water shows significant interannual variability,which is highly related to El Niño-Southern Oscillation(ENSO)events.The correlation coefficient between interannual change of SCS water volume proportion and the Niño 3.4 index is 0.75,with an increase of about 24%during El Niño years and a decrease of about−22%during La Niña years.The proportion also varies with the depth of particles released,showing two peaks at surface and subsurface depths of 5 m and 110 m,respectively. 展开更多
关键词 Indonesian Throughflow(ITF) South China Sea Throughflow(SCSTF) Makassar strait
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Response of sea-surface partial pressure of CO_(2)to ENSO events over the Taiwan Strait
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作者 Keyu Hu Qiong Wu +4 位作者 Tianqi Xiong Bo Li Peng Bai Zhenxin Ruan Chengcheng Yu 《Acta Oceanologica Sinica》 2025年第6期42-53,共12页
El Niño-Southern Oscillation(ENSO)affects the changes in ocean physical elements in Taiwan Strait(TWS)primarily by regulating the strength of the East Asian Winter Monsoon(EAWM)and the intrusion of the Kuroshio.A... El Niño-Southern Oscillation(ENSO)affects the changes in ocean physical elements in Taiwan Strait(TWS)primarily by regulating the strength of the East Asian Winter Monsoon(EAWM)and the intrusion of the Kuroshio.Additionally,the fluctuating impact between nutrient-poor seawater with high dissolved inorganic carbon(DIC)that infiltrates owing to the Kuroshio during El Niño phases and nutrient-rich seawater with low DIC from the South China Sea(SCS)carried by the EAWM during La Niña phases determines the nutrient content in TWS,thereby sculpting appropriate or unsuitable biochemical environment.In this study,based on high-resolution sea-surface partial pressure of carbon dioxide(pCO_(2))data,we investigate the relationship between pCO_(2)level of TWS and ENSO events in winter.The physical mechanisms affecting the anomalous distribution of pCO_(2)level during ENSO are also explored.Stepwise regression was employed to identify the optimal influencing factors for modeling pCO_(2).Results indicate a significant positive correlation between Niño3.4 index and pCO_(2),which is significantly influenced by factors such as sea-surface temperature(SST),chlorophyll-a(Chl a),and DIC.These are related to the anomalously strong Kuroshio intrusion and weaker EAWM during El Niño years.It brings a large amount of high SST water with low nutrient concentration and high DIC,which is detrimental to CO_(2)dissolution and phytoplankton growth over the TWS,leading to an increase in pCO_(2).Conversely,pCO_(2)level is significantly low under the influence of SCS seawater during La Niña years.Based on the characterization of the pCO_(2)level response to ENSO,the carbon balance at TWS can be explored. 展开更多
关键词 sea-surface partial pressure of CO_(2) El Niño-Southern Oscillation Taiwan strait East Asian Winter Monsoon KUROSHIO
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SMILE与FS-LASIK术后24小时内视力恢复与自觉症状的比较
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作者 李莹 赵点 周春阳 《中医眼耳鼻喉杂志》 2025年第1期43-47,共5页
目的比较飞秒激光小切口角膜基质透镜取出术(SMILE)和飞秒激光制瓣准分子激光原位角膜磨镶术(FS-LASIK)术后24h内裸眼视力与自觉症状,探讨两种手术方式术后极早期恢复的差异。方法根据纳排标准,纳入2022年11月01日到2022年12月31日于成... 目的比较飞秒激光小切口角膜基质透镜取出术(SMILE)和飞秒激光制瓣准分子激光原位角膜磨镶术(FS-LASIK)术后24h内裸眼视力与自觉症状,探讨两种手术方式术后极早期恢复的差异。方法根据纳排标准,纳入2022年11月01日到2022年12月31日于成都中医大银海眼科医院屈光手术科行SMILE或FS-LASIK手术矫正屈光不正的患者89例160眼,根据手术方式将其分为SMILE组(57例101眼)、FS-LASIK组(32例59眼)。分别比较两组患者术后1 h、2 h、4 h、24h时裸眼视力与自觉症状评分。采用SPSS 26.0软件对所收集的数据进行分析处理。结果术后1 h到术后24 h,两组患者裸眼视力稳步提升,且在术后1 h、2 h、4 h时FS-LASIK组裸眼视力恢复优于SMILE组(P<0.05),术后24 h时两组患者裸眼视力无明显差异;比较两组患者自觉症状积分,术后2 h、4 h、24 h时FS-LASIK组患者术眼自觉症状总积分明显高于SMILE组(P<0.05);术后24 h内所有时间点FS-LASIK组疼痛评分更高;两组患者术后24 h内各时间点术眼干涩感评分无统计学差异;两组患者术后2 h、4 h时术眼畏光及流泪评分有统计学差异(P<0.05),FS-LASIK组术眼畏光、流泪症状均更为明显。结论术后4 h之内,行FS-LASIK患者UCVA恢复优于行SMILE者,术后24 h时两组UCVA未见明显差异;但术后24 h内SMILE组总体自觉症状较FS-LASIK更轻。 展开更多
关键词 飞秒激光制瓣准分子激光原位角膜磨镶术 飞秒激光小切口角膜基质透镜取出术 早期视力 自觉症状
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互信息与遗传算法融合的多维分类特征选择算法
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作者 李二超 张宝新 +2 位作者 贾彬彬 包寅寅 杨宏强 《计算机工程与应用》 北大核心 2026年第5期162-177,共16页
多维分类(multi-dimensional classification,MDC)模型在处理高维特征时面临计算效率与泛化性能的挑战。特征选择通过筛选有效特征子集,可同时降低维度并提升分类器性能。已有的MDC研究主要集中于显式地建模类空间之间的依赖关系,而面向... 多维分类(multi-dimensional classification,MDC)模型在处理高维特征时面临计算效率与泛化性能的挑战。特征选择通过筛选有效特征子集,可同时降低维度并提升分类器性能。已有的MDC研究主要集中于显式地建模类空间之间的依赖关系,而面向MDC的特征选择方法仍需深入探索。针对MDC数据的特点,设计了一种互信息与遗传算法融合的多维分类特征选择算法MIGA(multi-dimensional classification feature selection algorithm based on fusion of mutual information and genetic algorithm)。该算法设计基于类空间综合相关性的种群初始化策略,以增加种群的多样性并加速收敛;提出自适应变异策略,依据特征综合得分动态调整变异概率以平衡全局探索与局部开发能力;融合MDC三项指标构建负加权和形式的适应度函数以适配GA优化框架。在10个MDC数据集上的实验结果表明:相较于特征映射降维方法(PCA、MDS)、监督式MDC降维方法SDeM(supervised dimensionality reduction for MDC)以及专用于MDC的过滤式特征选择算法MIFS(mutual information feature selection),MIGA所获特征子集显著提升了多维分类模型的泛化性能。 展开更多
关键词 机器学习 多维分类(MDC) 特征选择(fs) 遗传算法(GA) 互信息
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FS-C型固化土碳化特性及环境适应性研究
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作者 贾沼霖 赵启翔 +1 位作者 贺帅奇 张效旭 《海河水利》 2025年第10期85-93,共9页
针对FS-C型固化剂(脱硫石膏∶硅粉∶水泥=2∶1∶1)固化工程渣土碳化性能及环境适应性展开研究,通过自制碳化装置,模拟不同施工工况,设置成型状态碳化和松散状态碳化2种方式,根据不同碳化时间,研究碳化对FS-C型固化土无侧限抗压强度、微... 针对FS-C型固化剂(脱硫石膏∶硅粉∶水泥=2∶1∶1)固化工程渣土碳化性能及环境适应性展开研究,通过自制碳化装置,模拟不同施工工况,设置成型状态碳化和松散状态碳化2种方式,根据不同碳化时间,研究碳化对FS-C型固化土无侧限抗压强度、微观结构的影响。研究表明,成型碳化3~6 h对FS-C型固化土中后期强度提升有促进作用,松散状态碳化对FS-C型固化土强度发展具有抑制作用。在此基础上,对FS-C型固化土水稳性、抗离子侵蚀性、抗冻性试验展开研究,根据水稳定性系数、无侧限抗压强度指标研究其适应的环境,研究表明FS-C型固化土碳化后,在纯水和盐水养护下强度下降明显,而在冻融循环作用下强度表现较为优异。施工建议:FS-C型固化土适合在施工成型后进行碳化,且不适用在沿海和多雨地区,适用于寒冷地区。本研究可为固化土的碳化和工程实际应用提供理论基础。 展开更多
关键词 fs-C型固化土 碳化时间 碳化压力 无侧限抗压强度 水稳性 冻融循环
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