Straighthead disease is a physiological disorder in rice and the most widespread non-fungal disease of the U.S. straighthead greatly reduces rice yield. Straighthead studies were most conducted in the artificial field...Straighthead disease is a physiological disorder in rice and the most widespread non-fungal disease of the U.S. straighthead greatly reduces rice yield. Straighthead studies were most conducted in the artificial field which was Arsenic amendment plots due to the similarity of straighthead symptoms of Arsenic damage. To evaluate the straighthead disease resistance in natural condition, 46 rice lines, including 24 inbred lines and 22 hybrid combinations which were developed from breading program, were chosen for straighthead and agronomy characters testing in a native soil where straighthead naturally induced in the past years at University of Arkansas at Pine Bluff (UAPB), Arkansas in 2011 and 2012. The straighthead of rice were rated from 0 to 9 levels, and 0 was the least straighthead disorder and 9 was the most severe straighthead disorder. Cocodrie (CCDR) was used as the check in this study. The results of 2011 showed that the yields of 8 entries were higher than the check CCDR. The straighthead scores of these 8 entries were 0 - 1 which indicated their resistance to straighthead. The rest of the entries had lower yields than check CCDR with straighthead scores 5 - 6 which indicated their moderate susceptibility to straighthead. The yields were negatively related to the straighthead scores (r = -0.9178, P < 0.01). The results of 2012 showed that the yields of 9 hybrids and 6 inbred entries were higher than the check CCDR. The straighthead scores of top 12 entries were 0 - 2 which indicated that they were resistant to straighthead and 3 entries with straighthead score of 3 which indicated they were moderately resistant to straighthead. The rest 3 hybrids and 4 inbred entries had lower yields than the check and their straighthead scores were 5 - 6 which indicated their moderately susceptible to straighthead. The yields was negatively related to the straighthead scores (r = -0.956, P < 0.01). Compared to CCDR, the yield of hybrids showed their straighthead resistance at some extent. The yields of top six entries, hybrid 33A/PB23, inbred 3053, hybrid 28s/PB22, 28s/PB24, 29s/PB24 and inbred 3222, were 10,376.4, 9991.4, 9566.7, 9334.3, 9183.9 and 9174.3 Kg/Ha, and 46.0%, 40.7%, 34.7%, 31.4%, 29.3%, and 29.5% higher than the check, respectively.展开更多
Arsenic(As)methylation in soils affects the environmental behavior of As,excessive accumulation of dimethylarsenate(DMA)in rice plants leads to straighthead disease and a serious drop in crop yield.Understanding the m...Arsenic(As)methylation in soils affects the environmental behavior of As,excessive accumulation of dimethylarsenate(DMA)in rice plants leads to straighthead disease and a serious drop in crop yield.Understanding the mobility and transformation of methylated arsenic in redox-changing paddy fields is crucial for food security.Here,soils including unarsenic contaminated(N-As),low-arsenic(L-As),medium-arsenic(M-As),and high-arsenic(H-As)soils were incubated under continuous anoxic,continuous oxic,and consecutive anoxic/oxic treatments respectively,to profile arsenic methylating process and microbial species involved in the As cycle.Under anoxic-oxic(A-O)treatment,methylated arsenic was significantly increased once oxygen was introduced into the incubation system.The methylated arsenic concentrations were up to 2-24 times higher than those in anoxic(A),oxic(O),and oxic-anoxic(O-A)treatments,under which arsenic was methylated slightly and then decreased in all four As concentration soils.In fact,the most plentiful arsenite S-adenosylmethionine methyltransferase genes(arsM)contributed to the increase in As methylation.Proteobacteria(40.8%-62.4%),Firmicutes(3.5%-15.7%),and Desulfobacterota(5.3%-13.3%)were the major microorganisms related to this process.These microbial increasedmarkedly and played more important roles after oxygen was introduced,indicating that they were potential keystone microbial groups for As methylation in the alternating anoxic(flooding)and oxic(drainage)environment.The novel findings provided newinsights into the reoxidation-driven arsenic methylation processes and the model could be used for further risk estimation in periodically flooded paddy fields.展开更多
文摘Straighthead disease is a physiological disorder in rice and the most widespread non-fungal disease of the U.S. straighthead greatly reduces rice yield. Straighthead studies were most conducted in the artificial field which was Arsenic amendment plots due to the similarity of straighthead symptoms of Arsenic damage. To evaluate the straighthead disease resistance in natural condition, 46 rice lines, including 24 inbred lines and 22 hybrid combinations which were developed from breading program, were chosen for straighthead and agronomy characters testing in a native soil where straighthead naturally induced in the past years at University of Arkansas at Pine Bluff (UAPB), Arkansas in 2011 and 2012. The straighthead of rice were rated from 0 to 9 levels, and 0 was the least straighthead disorder and 9 was the most severe straighthead disorder. Cocodrie (CCDR) was used as the check in this study. The results of 2011 showed that the yields of 8 entries were higher than the check CCDR. The straighthead scores of these 8 entries were 0 - 1 which indicated their resistance to straighthead. The rest of the entries had lower yields than check CCDR with straighthead scores 5 - 6 which indicated their moderate susceptibility to straighthead. The yields were negatively related to the straighthead scores (r = -0.9178, P < 0.01). The results of 2012 showed that the yields of 9 hybrids and 6 inbred entries were higher than the check CCDR. The straighthead scores of top 12 entries were 0 - 2 which indicated that they were resistant to straighthead and 3 entries with straighthead score of 3 which indicated they were moderately resistant to straighthead. The rest 3 hybrids and 4 inbred entries had lower yields than the check and their straighthead scores were 5 - 6 which indicated their moderately susceptible to straighthead. The yields was negatively related to the straighthead scores (r = -0.956, P < 0.01). Compared to CCDR, the yield of hybrids showed their straighthead resistance at some extent. The yields of top six entries, hybrid 33A/PB23, inbred 3053, hybrid 28s/PB22, 28s/PB24, 29s/PB24 and inbred 3222, were 10,376.4, 9991.4, 9566.7, 9334.3, 9183.9 and 9174.3 Kg/Ha, and 46.0%, 40.7%, 34.7%, 31.4%, 29.3%, and 29.5% higher than the check, respectively.
基金supported by the Shandong Province Natural Science Foundation of Major Basic Research Program (No.ZR2020ZD34)the Key Projects of the National Natural Science Foundation of China (No.42230706)+3 种基金the National Natural Science Foundation of China (No.42307164)the China Postdoctoral Science Foundation (Nos.2023TQ0191 and 2023M732060)the Shandong Postdoctoral Science Foundation (No.SDBX2023041)and the Qingdao Postdoctoral Science Foundation (No.QDBSH20230202052).
文摘Arsenic(As)methylation in soils affects the environmental behavior of As,excessive accumulation of dimethylarsenate(DMA)in rice plants leads to straighthead disease and a serious drop in crop yield.Understanding the mobility and transformation of methylated arsenic in redox-changing paddy fields is crucial for food security.Here,soils including unarsenic contaminated(N-As),low-arsenic(L-As),medium-arsenic(M-As),and high-arsenic(H-As)soils were incubated under continuous anoxic,continuous oxic,and consecutive anoxic/oxic treatments respectively,to profile arsenic methylating process and microbial species involved in the As cycle.Under anoxic-oxic(A-O)treatment,methylated arsenic was significantly increased once oxygen was introduced into the incubation system.The methylated arsenic concentrations were up to 2-24 times higher than those in anoxic(A),oxic(O),and oxic-anoxic(O-A)treatments,under which arsenic was methylated slightly and then decreased in all four As concentration soils.In fact,the most plentiful arsenite S-adenosylmethionine methyltransferase genes(arsM)contributed to the increase in As methylation.Proteobacteria(40.8%-62.4%),Firmicutes(3.5%-15.7%),and Desulfobacterota(5.3%-13.3%)were the major microorganisms related to this process.These microbial increasedmarkedly and played more important roles after oxygen was introduced,indicating that they were potential keystone microbial groups for As methylation in the alternating anoxic(flooding)and oxic(drainage)environment.The novel findings provided newinsights into the reoxidation-driven arsenic methylation processes and the model could be used for further risk estimation in periodically flooded paddy fields.
