The Stokes–Einstein–Debye(SED) relation in TIP5P water is tested with the original formula and its variants within the temperature range 240–390 K. The results indicate that although the variants explicitly break d...The Stokes–Einstein–Debye(SED) relation in TIP5P water is tested with the original formula and its variants within the temperature range 240–390 K. The results indicate that although the variants explicitly break down, the original SED relation is almost valid. Compared with the Stokes–Einstein relation, no explicit decoupling is observed in translational and rotational motion. Variation of the effective hydrodynamic radius is critical to testing the validity of the SED relation.展开更多
The aggressive nature and high mortality rate of lung cancer underscore the imperative need for early diagnosis of the disease.Thus,aminopeptidase N(APN),a potential biomarker for lung cancer,should be thoroughly inve...The aggressive nature and high mortality rate of lung cancer underscore the imperative need for early diagnosis of the disease.Thus,aminopeptidase N(APN),a potential biomarker for lung cancer,should be thoroughly investigated in this context.This report describes the development of HA-apn,a novel near-infrared fluorescent probe,specifically engineered for the sensitive detection of endogenous APN.Characterized by its high selectivity,straightforward molecular architecture,and suitable optical properties,including a long-wavelength emission at 835 nm and a large Stokes shift of 285 nm,HA-apn had high efficacy in identifying overexpressed APN in tumor cells,which shows its potential in pinpointing malignancies.To further validate its applicability and effectiveness in facilitating the direct and enhanced visualization of pulmonary alterations,an in situ lung cancer mouse model was employed.Notably,HAapn was applied for in vivo imaging of APN activity in the lung cancer mouse model receiving the probe through aerosol inhalation,and rapid and precise diagnostic results were achieved within 30min postadministration.Overall,HA-apn can be applied as an effective,non-intrusive tool for the rapid and accurate detection of pulmonary conditions.展开更多
Red fluorescent proteins with large Stokes shift(LSS-RFPs)are advantageous for multicolor imaging applications that allow simultaneous visualizations of multiple biological events.But it is difficult to develop LSS-RF...Red fluorescent proteins with large Stokes shift(LSS-RFPs)are advantageous for multicolor imaging applications that allow simultaneous visualizations of multiple biological events.But it is difficult to develop LSS-RFPs by extending the emission wavelength of RFPs to far-red region.Here,we employed Forster resonance energy transfer(FRET)strategy to engineer the far-red fluorescent proteins with large Stokes shift.LSS-m Apple and LSS-mCherry were constructed by fusing HaloTag to m Apple and mCherry,allowing the fluorophore TMSi R to be connected to these RFPs.FRET between RFPs and TMSi R enabled them to apply the excitation of donor RFPs to emit far-red fluorescence of acceptor TMSi R.The Stokes shifts of LSS-m Apple and LSS-mCherry were 97 nm and 75 nm,respectively.The high FRET efficiency of LSS-mCherry(E_(FRET)=83.7%)can greatly reduce the fluorescence from the donor channel,which did not affect co-imaging with mCherry.In addition,LSS-mCherry also showed excellent photostability(t_(1/2)=449.3 s),enabling stable confocal fluorescence imaging for 15 min under continuous strong excitation.Furthermore,LSS-mCherry was applied for fluorescence labeling and imaging of the nucleus,mitochondria,lysosomes,and endoplasmic reticulum in living cells.Finally,we applied LSS-mCherry to perform multi-color bioimaging of 2–4 channels,and there was no obvious crosstalk between these channels.展开更多
The lattice dynamics of a high Al composition semiconductor alloy,Al_(0.86)Ga_(0.14)N,in comparison with intrinsic GaN and AlN are studied by Stokes/anti-Stokes Raman spectroscopy in the temperature range of 85–823 K...The lattice dynamics of a high Al composition semiconductor alloy,Al_(0.86)Ga_(0.14)N,in comparison with intrinsic GaN and AlN are studied by Stokes/anti-Stokes Raman spectroscopy in the temperature range of 85–823 K.The phonon anharmonic effect in Al_(0.86)Ga_(0.14)N is found to be stronger than that in GaN,revealing low thermal conductivity in the semiconductor alloy.Multi-phonon coupling behavior is analyzed by both Stokes Raman and anti-Stokes Raman spectroscopy.It is interesting to find that the anti-Stokes scattering exhibits stronger three-phonon coupling than the Stokes scattering,which may be due to the fact that the anti-stokes scattering process is generated from an excited state and the scattered photons have higher energies.The Stokes/anti-Stokes temperature correction factor β for Raman modes in Al_(0.86)Ga_(0.14)N alloy are all smaller than those of the corresponding intrinsic modes in GaN and AlN.The reasons for the difference in b can be attributed to three aspects,including the equipment setups,materials properties(the binding energy)and the coupling strength of Raman scattering and the sample.展开更多
Ocean waves and Stokes drift are generated by typhoons.This study investigated the characteristics of ocean waves and wave-induced Stokes drift and their effects during Typhoon Mangkhut using European Centre for Mediu...Ocean waves and Stokes drift are generated by typhoons.This study investigated the characteristics of ocean waves and wave-induced Stokes drift and their effects during Typhoon Mangkhut using European Centre for MediumRange Weather Forecasts(ECMWF)ERA5 datasets and observational data.The results revealed that the typhoon generated intense cyclones and huge typhoon waves with a maximum wind speed of 45 m/s,a minimum pressure of955 h Pa,and a maximum significant wave height of 12 m.The Stokes drift caused by typhoon waves exceeded 0.6m/s,the Stokes depth scale exceeded 18 m,and the maximum Stokes transport reached 6 m^(2)/s.The spatial distribution of 10-m wind speed,typhoon wave height,Stokes drift,Stokes depth,and Stokes transport during the typhoon was highly correlated with the typhoon track.The distribution along the typhoon track showed significant zonal asymmetry,with greater intensity on the right side of the typhoon track than on the left side.These findings provide important insights into the impact of typhoons on ocean waves and Stokes drift,thus improving our understanding of the interactions between typhoons and the ocean environment.This study also investigated the contribution of Stokes transport to the total net transport during typhoons using Ekman-Stokes Numbers as a comparative measure.The results indicated that the ratio of Stokes transport to the total net transport reached up to 50%within the typhoon radius,while it was approximately 30%outside the radius.Strong Stokes transport induced by typhoon waves led to divergence in the transport direction,which resulted in upwelling of the lower ocean as a compensation current.Thus,Stokes transport played a crucial role in the vertical mixing of the ocean during typhoons.The findings suggested that Stokes transport should be paid more attention to,particularly in high latitude ocean regions,where strong winds can amplify its effects.展开更多
The stability analysis of a finite Stokes layer is of practical importance in flow control. In the present work, the instantaneous stability of a finite Stokes layer with layer interactions is studied via a linear sta...The stability analysis of a finite Stokes layer is of practical importance in flow control. In the present work, the instantaneous stability of a finite Stokes layer with layer interactions is studied via a linear stability analysis of the frozen phases of the base flow. The oscillations of two plates can have different velocity amplitudes, initial phases, and frequencies. The effects of the Stokes-layer interactions on the stability when two plates oscillate synchronously are analyzed. The growth rates of two most unstable modes when δ < 0.12 are almost equal, and δ = δ*/h*, where δ*and h*are the Stokes-layer thickness and the half height of the channel, respectively. However, their vorticities are different. The vorticity of the most unstable mode is symmetric, while the other is asymmetric. The Stokes-layer interactions have a destabilizing effect on the most unstable mode when δ < 0.68, and have a stabilizing effect when δ > 0.68. However, the interactions always have a stabilizing effect on the other unstable mode. It is explained that one of the two unstable modes has much higher dissipation than the other one when the Stokes-layer interactions are strong. We also find that the stability of the Stokes layer is closely related to the inflectional points of the base-flow velocity profile. The effects of inconsistent velocity-amplitude, initial phase, and frequency of the oscillations on the stability are analyzed. The energy of the most unstable eigenvector is mainly distributed near the plate of higher velocity amplitude or higher oscillation frequency. The effects of the initial phase difference are complicated because the base-flow velocity is extremely sensitive to the initial phase.展开更多
As a hydrolase,chymotrypsin(CHT)is involved in many physiological activities,and its abnormal activity is closely related to diabetes,pancreatic fibrosis,chronic pancreatitis and pancreatic cancer.In this work,an inno...As a hydrolase,chymotrypsin(CHT)is involved in many physiological activities,and its abnormal activity is closely related to diabetes,pancreatic fibrosis,chronic pancreatitis and pancreatic cancer.In this work,an innovative long-wavelength emission fluorescent probe TCF-CHT was designed and synthesized for the high specificity detection of CHT,which utilized TCF-OH and a mimetic peptide substrate 4-bromobutyryl as chromogenic group and recognition group,respectively.TCF-CHT exhibited excellent selectivity and eye-catching sensitivity(8.91 ng/m L)towards CHT,“off-on”long-wavelength emission at 670 nm and large Stokes shift(140 nm).Furthermore,the successful fulfillment and perfect performance in imaging endogenous CHT in complex organisms(P815 cells,HepG2 cells,zebrafish and tumor-bearing mice)verified its potential as a powerful tool for the recognition of CHT in complicated biological environments.展开更多
The effect of Stokes drift production(SDP),which includes Coriolis-Stokes forcing,Langmuir circulation,and Craik-Lei-bovich vortexes,on the upper ocean during typhoon passage in the Bohai Sea(BS),China,is investigated...The effect of Stokes drift production(SDP),which includes Coriolis-Stokes forcing,Langmuir circulation,and Craik-Lei-bovich vortexes,on the upper ocean during typhoon passage in the Bohai Sea(BS),China,is investigated by using a coupled wave-current model.The role of SDP in turbulent mixing and the further dynamics during the entire typhoon period are comprehensively stud-ied.Experimental results show that SDP greatly increases turbulent mixing at all depths under typhoon conditions by up to seven times that under normal weather conditions.SDP generally strengthens sea surface cooling by more than 0.4℃,with the maximum reduction in sea surface temperature(SST)at the during-typhoon stage exceeding 2℃,which is approximately seven times larger than that under normal weather conditions.The SDP-induced decrease in current speed can exceed 0.2ms^(-1),and the change in current direction is generally opposite the wind direction.These results suggest that Stokes drift depresses the effect of strong winds on currents by intensifying turbulent mixing.Mixed layer depth(MLD)is distinctly increased by O(1)during typhoons due to SDP and can deepen by more than 5m.In addition,the continuous effects of SDP on SST,current,and MLD at the after-typhoon stage indi-cate a hysteretic response between SDP and typhoon actions.展开更多
We study the global existence and uniqueness of a strong solution to the kinetic thermomechanical Cucker-Smale(for short,TCS) model coupled with Stokes equations in the whole space.The coupled system consists of the k...We study the global existence and uniqueness of a strong solution to the kinetic thermomechanical Cucker-Smale(for short,TCS) model coupled with Stokes equations in the whole space.The coupled system consists of the kinetic TCS equation for a particle ensemble and the Stokes equations for a fluid via a drag force.In this paper,we present a complete analysis of the existence of global-in-time strong solutions to the coupled model without any smallness restrictions on the initial data.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 12104502)the Natural Science Foundation of Sichuan Province (Grant No. 2023YFG0308)the Fundamental Research Funds for the Central Universities (Grant No. 24CAFUC03057)。
文摘The Stokes–Einstein–Debye(SED) relation in TIP5P water is tested with the original formula and its variants within the temperature range 240–390 K. The results indicate that although the variants explicitly break down, the original SED relation is almost valid. Compared with the Stokes–Einstein relation, no explicit decoupling is observed in translational and rotational motion. Variation of the effective hydrodynamic radius is critical to testing the validity of the SED relation.
基金supported by the National Natural Science Foundation of China(Nos.22004046 and 22074052)the Science and Technology Developing Foundation of Jilin Province of China(Nos.20240404044ZP,20230101033JC and 20220505015ZP).
文摘The aggressive nature and high mortality rate of lung cancer underscore the imperative need for early diagnosis of the disease.Thus,aminopeptidase N(APN),a potential biomarker for lung cancer,should be thoroughly investigated in this context.This report describes the development of HA-apn,a novel near-infrared fluorescent probe,specifically engineered for the sensitive detection of endogenous APN.Characterized by its high selectivity,straightforward molecular architecture,and suitable optical properties,including a long-wavelength emission at 835 nm and a large Stokes shift of 285 nm,HA-apn had high efficacy in identifying overexpressed APN in tumor cells,which shows its potential in pinpointing malignancies.To further validate its applicability and effectiveness in facilitating the direct and enhanced visualization of pulmonary alterations,an in situ lung cancer mouse model was employed.Notably,HAapn was applied for in vivo imaging of APN activity in the lung cancer mouse model receiving the probe through aerosol inhalation,and rapid and precise diagnostic results were achieved within 30min postadministration.Overall,HA-apn can be applied as an effective,non-intrusive tool for the rapid and accurate detection of pulmonary conditions.
基金supported by the National Natural Science Foundation of China(Nos.22225806,22078314,22278394,22378385)Dalian Institute of Chemical Physics(Nos.DICPI202142,DICPI202436)。
文摘Red fluorescent proteins with large Stokes shift(LSS-RFPs)are advantageous for multicolor imaging applications that allow simultaneous visualizations of multiple biological events.But it is difficult to develop LSS-RFPs by extending the emission wavelength of RFPs to far-red region.Here,we employed Forster resonance energy transfer(FRET)strategy to engineer the far-red fluorescent proteins with large Stokes shift.LSS-m Apple and LSS-mCherry were constructed by fusing HaloTag to m Apple and mCherry,allowing the fluorophore TMSi R to be connected to these RFPs.FRET between RFPs and TMSi R enabled them to apply the excitation of donor RFPs to emit far-red fluorescence of acceptor TMSi R.The Stokes shifts of LSS-m Apple and LSS-mCherry were 97 nm and 75 nm,respectively.The high FRET efficiency of LSS-mCherry(E_(FRET)=83.7%)can greatly reduce the fluorescence from the donor channel,which did not affect co-imaging with mCherry.In addition,LSS-mCherry also showed excellent photostability(t_(1/2)=449.3 s),enabling stable confocal fluorescence imaging for 15 min under continuous strong excitation.Furthermore,LSS-mCherry was applied for fluorescence labeling and imaging of the nucleus,mitochondria,lysosomes,and endoplasmic reticulum in living cells.Finally,we applied LSS-mCherry to perform multi-color bioimaging of 2–4 channels,and there was no obvious crosstalk between these channels.
基金supported by the National Natural Science Foundation of China(Grant No.12374013)the Fundamental Research Funds for the Central Universities(Grant No.2020SCUNL107).
文摘The lattice dynamics of a high Al composition semiconductor alloy,Al_(0.86)Ga_(0.14)N,in comparison with intrinsic GaN and AlN are studied by Stokes/anti-Stokes Raman spectroscopy in the temperature range of 85–823 K.The phonon anharmonic effect in Al_(0.86)Ga_(0.14)N is found to be stronger than that in GaN,revealing low thermal conductivity in the semiconductor alloy.Multi-phonon coupling behavior is analyzed by both Stokes Raman and anti-Stokes Raman spectroscopy.It is interesting to find that the anti-Stokes scattering exhibits stronger three-phonon coupling than the Stokes scattering,which may be due to the fact that the anti-stokes scattering process is generated from an excited state and the scattered photons have higher energies.The Stokes/anti-Stokes temperature correction factor β for Raman modes in Al_(0.86)Ga_(0.14)N alloy are all smaller than those of the corresponding intrinsic modes in GaN and AlN.The reasons for the difference in b can be attributed to three aspects,including the equipment setups,materials properties(the binding energy)and the coupling strength of Raman scattering and the sample.
基金financially supported by the National Key Research and Development Program of China(Grant No.2021YFB2601100)the National Natural Science Foundation of China(Grant No.52171246)+4 种基金The Belt and Road Special Foundation of the State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering(Grant No.2019491911)the Open Research Foundation of the State Key Laboratory of Coastal and Offshore Engineering,Dalian University of Technology(Grant No.LP2005)the Science and Technology Innovation Program of Hunan Province(Grant No.2023RC3136)the Natural Science Foundation of Hunan Province(Grant No.2022JJ20041)Educational Science Foundation of Hunan Province(Grant No.23A0265)。
文摘Ocean waves and Stokes drift are generated by typhoons.This study investigated the characteristics of ocean waves and wave-induced Stokes drift and their effects during Typhoon Mangkhut using European Centre for MediumRange Weather Forecasts(ECMWF)ERA5 datasets and observational data.The results revealed that the typhoon generated intense cyclones and huge typhoon waves with a maximum wind speed of 45 m/s,a minimum pressure of955 h Pa,and a maximum significant wave height of 12 m.The Stokes drift caused by typhoon waves exceeded 0.6m/s,the Stokes depth scale exceeded 18 m,and the maximum Stokes transport reached 6 m^(2)/s.The spatial distribution of 10-m wind speed,typhoon wave height,Stokes drift,Stokes depth,and Stokes transport during the typhoon was highly correlated with the typhoon track.The distribution along the typhoon track showed significant zonal asymmetry,with greater intensity on the right side of the typhoon track than on the left side.These findings provide important insights into the impact of typhoons on ocean waves and Stokes drift,thus improving our understanding of the interactions between typhoons and the ocean environment.This study also investigated the contribution of Stokes transport to the total net transport during typhoons using Ekman-Stokes Numbers as a comparative measure.The results indicated that the ratio of Stokes transport to the total net transport reached up to 50%within the typhoon radius,while it was approximately 30%outside the radius.Strong Stokes transport induced by typhoon waves led to divergence in the transport direction,which resulted in upwelling of the lower ocean as a compensation current.Thus,Stokes transport played a crucial role in the vertical mixing of the ocean during typhoons.The findings suggested that Stokes transport should be paid more attention to,particularly in high latitude ocean regions,where strong winds can amplify its effects.
基金Project supported by the National Natural Science Foundation of China (No. 11402211)。
文摘The stability analysis of a finite Stokes layer is of practical importance in flow control. In the present work, the instantaneous stability of a finite Stokes layer with layer interactions is studied via a linear stability analysis of the frozen phases of the base flow. The oscillations of two plates can have different velocity amplitudes, initial phases, and frequencies. The effects of the Stokes-layer interactions on the stability when two plates oscillate synchronously are analyzed. The growth rates of two most unstable modes when δ < 0.12 are almost equal, and δ = δ*/h*, where δ*and h*are the Stokes-layer thickness and the half height of the channel, respectively. However, their vorticities are different. The vorticity of the most unstable mode is symmetric, while the other is asymmetric. The Stokes-layer interactions have a destabilizing effect on the most unstable mode when δ < 0.68, and have a stabilizing effect when δ > 0.68. However, the interactions always have a stabilizing effect on the other unstable mode. It is explained that one of the two unstable modes has much higher dissipation than the other one when the Stokes-layer interactions are strong. We also find that the stability of the Stokes layer is closely related to the inflectional points of the base-flow velocity profile. The effects of inconsistent velocity-amplitude, initial phase, and frequency of the oscillations on the stability are analyzed. The energy of the most unstable eigenvector is mainly distributed near the plate of higher velocity amplitude or higher oscillation frequency. The effects of the initial phase difference are complicated because the base-flow velocity is extremely sensitive to the initial phase.
基金financial support provided by National Natural Science Foundation of China(Nos.21775005,41430641 and 41140032)the Start-Up Fund of Qingdao University of Science and Technology(No.12030430010883)。
文摘As a hydrolase,chymotrypsin(CHT)is involved in many physiological activities,and its abnormal activity is closely related to diabetes,pancreatic fibrosis,chronic pancreatitis and pancreatic cancer.In this work,an innovative long-wavelength emission fluorescent probe TCF-CHT was designed and synthesized for the high specificity detection of CHT,which utilized TCF-OH and a mimetic peptide substrate 4-bromobutyryl as chromogenic group and recognition group,respectively.TCF-CHT exhibited excellent selectivity and eye-catching sensitivity(8.91 ng/m L)towards CHT,“off-on”long-wavelength emission at 670 nm and large Stokes shift(140 nm).Furthermore,the successful fulfillment and perfect performance in imaging endogenous CHT in complex organisms(P815 cells,HepG2 cells,zebrafish and tumor-bearing mice)verified its potential as a powerful tool for the recognition of CHT in complicated biological environments.
基金supported by the National Natural Science Foundation of China(No.42176020)the National Key Research and Development Program(No.2022 YFC3105002).
文摘The effect of Stokes drift production(SDP),which includes Coriolis-Stokes forcing,Langmuir circulation,and Craik-Lei-bovich vortexes,on the upper ocean during typhoon passage in the Bohai Sea(BS),China,is investigated by using a coupled wave-current model.The role of SDP in turbulent mixing and the further dynamics during the entire typhoon period are comprehensively stud-ied.Experimental results show that SDP greatly increases turbulent mixing at all depths under typhoon conditions by up to seven times that under normal weather conditions.SDP generally strengthens sea surface cooling by more than 0.4℃,with the maximum reduction in sea surface temperature(SST)at the during-typhoon stage exceeding 2℃,which is approximately seven times larger than that under normal weather conditions.The SDP-induced decrease in current speed can exceed 0.2ms^(-1),and the change in current direction is generally opposite the wind direction.These results suggest that Stokes drift depresses the effect of strong winds on currents by intensifying turbulent mixing.Mixed layer depth(MLD)is distinctly increased by O(1)during typhoons due to SDP and can deepen by more than 5m.In addition,the continuous effects of SDP on SST,current,and MLD at the after-typhoon stage indi-cate a hysteretic response between SDP and typhoon actions.
基金supported by the National Natural Science Foundation of China (12001033)。
文摘We study the global existence and uniqueness of a strong solution to the kinetic thermomechanical Cucker-Smale(for short,TCS) model coupled with Stokes equations in the whole space.The coupled system consists of the kinetic TCS equation for a particle ensemble and the Stokes equations for a fluid via a drag force.In this paper,we present a complete analysis of the existence of global-in-time strong solutions to the coupled model without any smallness restrictions on the initial data.
