0引言我院新生儿重症监护病房（neonatal intensive care unit,NICU）使用的是德国斯蒂芬Stephanie呼吸机,它适用于婴幼儿和体质量小于20 kg的小儿,具有高频振荡通气功能。高频通气是利用高频率的振动促进对流及气体扩散、弥散过程[1],...0引言我院新生儿重症监护病房（neonatal intensive care unit,NICU）使用的是德国斯蒂芬Stephanie呼吸机,它适用于婴幼儿和体质量小于20 kg的小儿,具有高频振荡通气功能。高频通气是利用高频率的振动促进对流及气体扩散、弥散过程[1],配备有多种适用于新生儿患者的呼吸模式。它使用的是吸气和呼气端同步控制的比例阀系统,展开更多
Dracocephalum palmatum Stephan is a medicinal plant traditionally used by nomadic people in Eastern Russia;however,research on this plant is currently limited.Recently,although studies have been conducted on the const...Dracocephalum palmatum Stephan is a medicinal plant traditionally used by nomadic people in Eastern Russia;however,research on this plant is currently limited.Recently,although studies have been conducted on the constituents of this plant and their antioxidant effects,data on its various pharmacological activities are still lacking.Thus,this study examined the anticancer potential of the dried leaves of D.palmatum S.(DpL)using human prostate cancer PC-3 cells.The antioxidant potential of DpL was evaluated by estimating the total flavonoid and total phenolic content(TFC and TPC,respectively).Additionally,we investigated the effects of the DpL ethyl acetate fraction(DpLE)on cell proliferation,intracellular reactive oxygen species(ROS)generation,apoptosis,and cell cycle arrest in this cell line.The expression levels of superoxide dismutase(SOD)-1,SOD-2,B-cell lymphoma 2(Bcl-2)and Bcl-2 associated X(Bax)ratio,phospho-protein kinase B(p-AKT),cleaved caspase-8,poly adenosine diphosphate(ADP)ribose polymerase(PARP),and cleaved-PARP were evaluated by western blotting.The results indicated that DpLE causes apoptosis and exerts intracellular ROS-independent anticancer effects on prostate cancer cells,associated with increased SOD-2,cleaved caspase-8,and cleaved-PARP expression and inhibited p-AKT signaling.Thus,DpLE may be a potential resource for the development of promising chemotherapeutic agents for prostate cancer.展开更多
本季Stephane Rol and保留了品牌独特的拖尾和精致的肩部设计,设计师说他把每条裙子都当作雕塑来制作,这一季他选择黑白纯色来突出裙装的廓形和剪裁,这些作品优美自然地就像是从写真簿直接搬上了T台.腿部独特的透视从视觉上拉长了双腿,...本季Stephane Rol and保留了品牌独特的拖尾和精致的肩部设计,设计师说他把每条裙子都当作雕塑来制作,这一季他选择黑白纯色来突出裙装的廓形和剪裁,这些作品优美自然地就像是从写真簿直接搬上了T台.腿部独特的透视从视觉上拉长了双腿,看起来高贵又性感.展开更多
Symbiotic microorganisms are essential for the physiological processes of herbivorous pests,including the pear lace bug Stephanitis nashi,which is known for causing extensive damage to garden plants and fruit trees du...Symbiotic microorganisms are essential for the physiological processes of herbivorous pests,including the pear lace bug Stephanitis nashi,which is known for causing extensive damage to garden plants and fruit trees due to its exceptional adaptability to diverse host plants.However,the specific functional effects of the microbiome on the adaptation of S.nashi to its host plants remains unclear.Here,we identified significant microbial changes in S.nashi on 2 different host plants,crabapple and cherry blossom,characterized by the differences in fungal diversity as well as bacterial and fungal community structures,with abundant correlations between bacteria or fungi.Consistent with the microbiome changes,S.nashi that fed on cherry blossom demonstrated decreased metabolites and downregulated key metabolic pathways,such as the arginine and mitogen-activated protein kinase signaling pathway,which were crucial for host plant adaptation.Furthermore,correlation analysis unveiled numerous correlations between differential microorganisms and differential metabolites,which were influenced by the interactions between bacteria or fungi.These differential bacteria,fungi,and associated metabolites may modify the key metabolic pathways in S.nashi,aiding its adaptation to different host plants.These results provide valuable insights into the alteration in microbiome and function of S.nashi adapted to different host plants,contributing to a better understanding of pest invasion and dispersal from a microbial perspective.展开更多
文摘0引言我院新生儿重症监护病房（neonatal intensive care unit,NICU）使用的是德国斯蒂芬Stephanie呼吸机,它适用于婴幼儿和体质量小于20 kg的小儿,具有高频振荡通气功能。高频通气是利用高频率的振动促进对流及气体扩散、弥散过程[1],配备有多种适用于新生儿患者的呼吸模式。它使用的是吸气和呼气端同步控制的比例阀系统,
文摘Dracocephalum palmatum Stephan is a medicinal plant traditionally used by nomadic people in Eastern Russia;however,research on this plant is currently limited.Recently,although studies have been conducted on the constituents of this plant and their antioxidant effects,data on its various pharmacological activities are still lacking.Thus,this study examined the anticancer potential of the dried leaves of D.palmatum S.(DpL)using human prostate cancer PC-3 cells.The antioxidant potential of DpL was evaluated by estimating the total flavonoid and total phenolic content(TFC and TPC,respectively).Additionally,we investigated the effects of the DpL ethyl acetate fraction(DpLE)on cell proliferation,intracellular reactive oxygen species(ROS)generation,apoptosis,and cell cycle arrest in this cell line.The expression levels of superoxide dismutase(SOD)-1,SOD-2,B-cell lymphoma 2(Bcl-2)and Bcl-2 associated X(Bax)ratio,phospho-protein kinase B(p-AKT),cleaved caspase-8,poly adenosine diphosphate(ADP)ribose polymerase(PARP),and cleaved-PARP were evaluated by western blotting.The results indicated that DpLE causes apoptosis and exerts intracellular ROS-independent anticancer effects on prostate cancer cells,associated with increased SOD-2,cleaved caspase-8,and cleaved-PARP expression and inhibited p-AKT signaling.Thus,DpLE may be a potential resource for the development of promising chemotherapeutic agents for prostate cancer.
基金supported by grants-in-aid from the National Natural Science Foundation of China(No.32301594)the Scientific Research Startup Project of Nanjing Forestry University(No.163010320 and 163010325).
文摘Symbiotic microorganisms are essential for the physiological processes of herbivorous pests,including the pear lace bug Stephanitis nashi,which is known for causing extensive damage to garden plants and fruit trees due to its exceptional adaptability to diverse host plants.However,the specific functional effects of the microbiome on the adaptation of S.nashi to its host plants remains unclear.Here,we identified significant microbial changes in S.nashi on 2 different host plants,crabapple and cherry blossom,characterized by the differences in fungal diversity as well as bacterial and fungal community structures,with abundant correlations between bacteria or fungi.Consistent with the microbiome changes,S.nashi that fed on cherry blossom demonstrated decreased metabolites and downregulated key metabolic pathways,such as the arginine and mitogen-activated protein kinase signaling pathway,which were crucial for host plant adaptation.Furthermore,correlation analysis unveiled numerous correlations between differential microorganisms and differential metabolites,which were influenced by the interactions between bacteria or fungi.These differential bacteria,fungi,and associated metabolites may modify the key metabolic pathways in S.nashi,aiding its adaptation to different host plants.These results provide valuable insights into the alteration in microbiome and function of S.nashi adapted to different host plants,contributing to a better understanding of pest invasion and dispersal from a microbial perspective.
