Starch-lipid inclusion complexes were used to regulate starch digestibility by changing the binding affinity of digestive enzymes such as α-amylase and α-glucosidase.However,when above a critical aggregation concent...Starch-lipid inclusion complexes were used to regulate starch digestibility by changing the binding affinity of digestive enzymes such as α-amylase and α-glucosidase.However,when above a critical aggregation concentration(CAC)of starch and lipid molecules,they tended to self-assemble into double helices or normal micelles respectively,rather than interacting with each other to form starch-lipid complexes.Thus,how to enhance the complexation efficiency under a dense system is the key approach for the improvement of starch bioaccessibility and nutritional function.Herein,based on the dimethyl sulfoxide(DMSO)reverse solvent method,the effect of water/DMSO ratios and starch chain structures on the interaction and assembly behaviors between starch and lecithin molecules in high concentrations were systematically investigated.The results revealed that water/DMSO in a ratio of 1.5:1 promoted the interactions between cavities of starch helices and long alkyl chains of lecithin molecules,with higher level of complexed lecithin content,relative crystallinity(RC),enthalpy(ΔH)(Tp>100℃),ordered-aggregation structures and resistant starch(RS)content.In this kind of solvent,starch samples with higher content of amylose and longer chains(degree of polymerization(DP)of B2 chains and B3+chains)is advantageous to the formation of VII-type inclusion starch-lecithin complexes,with a rise in RS from 14.17%to 43.64%.It provided an effective method to modulate starch digestibility under the dense system of starch and lecithin via modulating the water/DMSO ratios and starch fine structures and lay the foundation for the large-scale preparation and application of starch-lecithin complexes.展开更多
基金funded by the National Natural Science Foundation of China(NSFC)-Guangdong Joint Foundation Key Project(U22A20539)the National Key R&D Program of China(2021YFD2100101-03).
文摘Starch-lipid inclusion complexes were used to regulate starch digestibility by changing the binding affinity of digestive enzymes such as α-amylase and α-glucosidase.However,when above a critical aggregation concentration(CAC)of starch and lipid molecules,they tended to self-assemble into double helices or normal micelles respectively,rather than interacting with each other to form starch-lipid complexes.Thus,how to enhance the complexation efficiency under a dense system is the key approach for the improvement of starch bioaccessibility and nutritional function.Herein,based on the dimethyl sulfoxide(DMSO)reverse solvent method,the effect of water/DMSO ratios and starch chain structures on the interaction and assembly behaviors between starch and lecithin molecules in high concentrations were systematically investigated.The results revealed that water/DMSO in a ratio of 1.5:1 promoted the interactions between cavities of starch helices and long alkyl chains of lecithin molecules,with higher level of complexed lecithin content,relative crystallinity(RC),enthalpy(ΔH)(Tp>100℃),ordered-aggregation structures and resistant starch(RS)content.In this kind of solvent,starch samples with higher content of amylose and longer chains(degree of polymerization(DP)of B2 chains and B3+chains)is advantageous to the formation of VII-type inclusion starch-lecithin complexes,with a rise in RS from 14.17%to 43.64%.It provided an effective method to modulate starch digestibility under the dense system of starch and lecithin via modulating the water/DMSO ratios and starch fine structures and lay the foundation for the large-scale preparation and application of starch-lecithin complexes.
