Starch is an essential commodity for humans and other animals.Future demands require qualitative and quantitative improvement by crop and post-harvest engineering that calls for comprehensive actions requiring increas...Starch is an essential commodity for humans and other animals.Future demands require qualitative and quantitative improvement by crop and post-harvest engineering that calls for comprehensive actions requiring increased fundamental knowledge on starch biosynthesis,development of advanced breeding strategies,efficient farming,and well-adapted and up scalable extraction protocols for diverse starch products.Recent staggering progress in molecular breeding techniques,especially genome editing,have enabled generation of higher starch yield and special functional qualities required to support such advancement.However,this necessitates fundamental biochemical and mechanistic understanding of starch biosynthesis and the variegated starch crop germplasms,all of which are closely linked to the relationships between starch molecular structures and functionality of various starch types as directed by the different capabilities of starch crop genotypes.We here review starch biosynthesis and its genetic foundation with a focus on increasing nutritional and health-promoting value of starch especially through bioengineering of the high amylose trait.展开更多
Starch biosynthesis is a complex process that relies on the coordinated action of multiple enzymes.Resistant starch is not digested in the small intestine,thus preventing a rapid rise in the glycemic index.Starch synt...Starch biosynthesis is a complex process that relies on the coordinated action of multiple enzymes.Resistant starch is not digested in the small intestine,thus preventing a rapid rise in the glycemic index.Starch synthase 2a(SS2a)is a key enzyme in amylopectin biosynthesis that has significant effects on starch structure and properties.In this study,we identified an ss2a null mutant(M3-1413)with a single base mutation from an ethyl methane sulfonate(EMS)-mutagenized population of barley.The mutation was located at the 3'end of the first intron of the RNA splicing receptor(AG)site,and resulted in abnormal RNA splicing and two abnormal transcripts of ss2a,which caused the inactivation of the SS2a gene.The starch structure and properties were significantly altered in the mutant,with M3-1413 containing lower total starch and higher amylose and resistant starch levels.This study sheds light on the effect of barley ss2a null mutations on starch properties and will help to guide new applications of barley starch in the development of nutritious food products.展开更多
This work explores the development of biodegradable laminar composite foams for cushioning applications.The focus lies on overcoming the inherent brittleness of starch foams by incorporating various paper types as rei...This work explores the development of biodegradable laminar composite foams for cushioning applications.The focus lies on overcoming the inherent brittleness of starch foams by incorporating various paper types as rein-forcement.Tapioca starch and glutinous starch were blended in varying ratios(100:0–0:100)to optimize the base material’s properties.The morphology,density,flexural strength,and impact strength of these starch blends were evaluated.The results revealed a trade-off between impact strength and density,with increasing glutinous starch content favoring impact resistance but also leading to higher density.The optimal ratio of tapioca to glutinous starch for achieving maximumflexural strength and modulus was determined to be 60:40.Theflexural strength of the composite material at this ratio reached a peak value of 5.3±0.6 MPa,significantly surpassing theflexural strength of pure tapioca foam,which was measured to be 3.5±0.4 MPa.Building on this foundation,novel lami-nar composite foams were fabricated using the 60:40 starch blend reinforced with mulberry paper,kraft paper,and newsprint paper.To enhance the interfacial adhesion between the starch matrix and paper reinforcement,a silane coupling agent was employed at a 10 wt%loading on the paper.The incorporation of paper reinforcement into starch foams was found to enhance their mechanical properties.Specifically,flexural strength values increased from 5.3±0.6 MPa for the unreinforced starch foam to 6.8±0.6 MPa,8.1±0.9 MPa,and 7.4±0.1 MPa when reinforced with mulberry paper,kraft paper,and newsprint paper,respectively.Notably,kraft paper reinforcement led to the most enhancements inflexural strength,flexural modulus,and impact strength.This research paves the way for developing sustainable cushioning materials with competitive mechanical properties using bio-based resources like starch and paper.展开更多
With the promotion of potatoes as a staple food,the challenge lies in integrating them into traditional staple grain-based diets.As the primary component of grains,starch plays a crucial role,and its interactions with...With the promotion of potatoes as a staple food,the challenge lies in integrating them into traditional staple grain-based diets.As the primary component of grains,starch plays a crucial role,and its interactions with other heterogeneous starches could significantly influence their functional properties.Therefore,this study aimed to explore the role of substitution ratio(SR,0–100%)of potato starch(PS)in the compatibility with wheat starch(WS)from the perspective of microstructure and physicochemical characteristics using the simplex-centroid method.Results of scaning electron microscopy(SEM)revealed the network structure in SR 30%of PS possessed the smallest size and the most compact gel structure.A higher SR of PS increased the solubility and swelling power of the blended WS and improved its freeze-thaw stability.Peak viscosity and gel hardness of the blended WS exhibited an increasing trend as the SR of PS increased.With the increasing SR of PS,the blended WS exhibited an increased tanδvalue with decreased viscous and elastic moduli.There is a non-additive effect between PS and WS,which should be responsible for their difference between experimental and theoretical values.These findings could provide a new direction for understanding the compatibility interactions between different starches,and provide a theoretical guidance for the development of PS-based foods with desirable properties.展开更多
Jasmonate ZIM-domain(JAZ)proteins are key repressors of the jasmonate signaling pathway and are involved in plant stress responses.However,their roles in starch biosynthesis in cereal crops remain unclear.In this stud...Jasmonate ZIM-domain(JAZ)proteins are key repressors of the jasmonate signaling pathway and are involved in plant stress responses.However,their roles in starch biosynthesis in cereal crops remain unclear.In this study,we identified a locus associated with starch content on chromosome 5A by a genome-wide association study(GWAS).At this locus,a gene(TraesCS5A02G204900)encoding a JAZ protein(TaJAZ1)was found to be highly expressed in grains.CRISPR/Cas9-induced mutants were generated to investigate the role of TaJAZ1 in starch biosynthesis.Phenotypic characterization revealed significant alterations in starch granule size,crystallinity,and digestibility.Specifically,the two mutant lines(tajaz1-abd#1 and tajaz1-abd#2)exhibited increased total starch(12.5%and 17.6%,respectively),amylose(79.3%and 72.1%,respectively),resistant starch(88.5%and 96.8%,respectively),and grain yield per plant(103.8%and 58.8%,respectively).Furthermore,the mutation of TaJAZ1 significantly increased the expression levels of TaSBEI,TaAGPS1,TaAGPL1 and TaGBSSI,but decreased the expression levels of TaSSIIa,TaSSIIb and TaSBEIIa by binding to their promoters.Taken together,our results demonstrate that TaJAZ1 is a negative regulator of starch biosynthesis and grain yield.These findings not only provide novel insights into wheat starch biosynthesis regulation,but also contribute to potential genes for breeding wheat varieties of better quality and higher yield.展开更多
Dynamic changes of starch, amylose, sucrose contents and the activities of starch synthesis enzymes under shading treatments alter flowering were studied using two rice varieties IR72 （indica） and Nipponbare （japon...Dynamic changes of starch, amylose, sucrose contents and the activities of starch synthesis enzymes under shading treatments alter flowering were studied using two rice varieties IR72 （indica） and Nipponbare （japonica） as materials. Under shading treatments, the starch, amylose and sucrose contents decreased, while ADP-glucose pyrophosphorylase （ADPGPPase） activity only changed a little, soluble starch synthase activity and granule bound starch synthase activity decreased, soluble starch branching enzyme （SSBE, Q-enzyme） activity and granule bound starch branching enzyme （GBSBE, Q-enzyme） activity increased, and starch debranching enzyme （DBE, R-enzyme） activity varied with varieties. Correlation analyses showed that the changes of starch content were positively and significantly correlated with the changes of sucrose content in the weak light. Both ADPGPPase activity and SSBE activity were positively and significantly correlated with starch accumulation rate. It was implied that the decline of starch synthase activities was related to the decrease of starch content and the increase of the activity of starch branching enzyme played an important role in the decrease of the ratio of amylose to the total starch under the weak light.展开更多
To study the characteristics of tuber starchgranules,firstly,three kinds of common tuber starches were separated and characterized by gravitational field-flow fractionation(GrFFF)instrument,and the ratios of large and...To study the characteristics of tuber starchgranules,firstly,three kinds of common tuber starches were separated and characterized by gravitational field-flow fractionation(GrFFF)instrument,and the ratios of large and small starch granules A/B were calculated.Then,the liquid of peak A and peak B was collected and observed by scanning electron microscope(SEM)to verify the separation result of GrFFF.Secondly,the particle size distribution and crystal characteristics were characterized by particle size analyser,X-ray diffraction(X-ray)and fourier transform infrared spectroscopy(FT-IR).Three kinds of tuber starches all had the bimodal distribution in GrFFF.Peak A contained large particles,and peak B contained small particles.For yam starch,the ratio of starch granules A/B was much higher than 1;for sweet potato starch the ratio was approximately equal to 1;for tapioca starch,the ratio was much lower than 1.Moreover,the starch granules of sweet potato and tapioca were both A-type crystallinity,while yam starch granules were C-type crystallinity.GrFFF could be used to characterize and separate large and small granules of tuber starches and estimate the ratio of starch granules A/B.Yam starch was suitable for the development of slowly digestible starch(one kind of functional food),which could delay the occurrence of metabolic syndrome,diabetes and cardiovascular diseases.Tapioca starch was suitable for plant capsules,which were safer than gelatin capsule and could prevent the"poison capsule"incident from happening.展开更多
Background The synchronized absorption of amino acids(AAs)and glucose in the gut is crucial for effective AA utilization and protein synthesis in the body.The study investigated how the starch digestion rate and AA le...Background The synchronized absorption of amino acids(AAs)and glucose in the gut is crucial for effective AA utilization and protein synthesis in the body.The study investigated how the starch digestion rate and AA levels impact intestinal AA digestion,transport and metabolism,breast muscle protein metabolism,and growth in grower broilers.A total of 72021-day-old healthy male Arbor Acres Plus broilers were randomly assigned to 12 treatments,each with 6 replicates of 10 birds.The treatments comprised 3 different starch[corn:control,cassava:rapidly digestible starch(RDS),and pea:slowly digestible starch(SDS)]with 4 different AA levels[based on standardized ileal digestible lysine(SID Lys),0.92%,1.02%(as the standard),1.12%and 1.22%].Results An interaction between dietary starch sources and SID Lys levels significantly affected breast muscle yield(P=0.033).RDS and SDS diets,or SID Lys levels of 0.92%,1.02%,or 1.22%,significantly decreased the breast muscle yield of broilers in contrast to the corn starch diet with 1.12%SID Lys(P=0.033).The SID Lys levels of 1.12%and 1.22%markedly improved body weight(BW),body weight gain(BWG)from 22 to 42 days of age,and mRNA expression of y^(+)LAT1 and mTOR while reducing feed intake(FI)and feed/gain ratio(F/G)compared to the 0.92%SID Lys level(P<0.05).The SDS diet significantly decreased BW and BWG of broilers from 22 to 42 days of age,distal ileal starch digestibility,jejunal amylase and chymotrypsin activities,and mRNA expression of GLUT2 and y^(+)LAT1 compared to the corn starch diet(P<0.05).The RDS diet suppressed the breast muscle mass by down-regulating expression of mTOR,S6K1,and eIF4E and up-regulating expression of MuRF,CathepsinB,Atrogin-1,and M-calpain compared to the corn starch diet(P<0.05).Targeted metabolomics analysis revealed that the SDS diet significantly increased acetyl-CoA andα-ketoglutaric acid levels in the tricarboxylic acid(TCA)cycle(P<0.05)but decreased the ileal digestibility of Lys,Tyr,Leu,Asp,Ser,Gly,Pro,Arg,Ile,and Val compared to the corn starch group(P<0.05).Conclusion The SDS diet impaired broiler growth by reducing intestinal starch digestibility,which inhibited intestinal AA and glucose absorption and utilization,increased AA oxidation for energy supply,and lowered the efficiency of protein synthesis.Although the RDS diet resulted in growth performance similar to the corn starch diet,it reduced breast muscle mass by inhibiting protein synthesis and promoting degradation.展开更多
Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperat...Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation.However,whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown.In this study,we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence(DAP-Seq)approach.Electrophoretic mobility shift assay(EMSA)and dual-luciferase reporter assay(DLR)demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3,MaBAM2 and MaGWD1.Further analyses of yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and Luciferase complementation imaging(LCI)assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1,and this interaction weakened the MaERF11-mediated transcriptional repression capacity.Collectively,our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.展开更多
Transcription factors play critical roles in the regulation of gene expression during maize kernel development.The maize endosperm,a large storage organ,accounting for nearly 90%of the dry weight of mature kernels,ser...Transcription factors play critical roles in the regulation of gene expression during maize kernel development.The maize endosperm,a large storage organ,accounting for nearly 90%of the dry weight of mature kernels,serves as the primary site for starch storage.In this study,we identify an endosperm-specific EREB gene,ZmEREB167,which encodes a nucleus-localized EREB protein.Knockout of ZmEREB167 significantly increases kernel size and weight,as well as starch and protein content,compared with the wild type.In situ hybridization experiments show that ZmEREB167 is highly expressed in the BETL as well as PED regions of maize kernels.Dual-luciferase assays show that ZmEREB167 exhibits transcriptionally repressor activity in maize protoplasts.Transcriptome analysis reveals that a large number of genes are up-regulated in the Zmereb167-C1 mutant compared with the wild type,including key genetic factors such as ZmMRP-1 and ZmMN1,as well as multiple transporters involved in maize endosperm development.Integration of RNA-seq and ChIP-seq results identify 68 target genes modulated by ZmEREB167.We find that ZmEREB167 directly targets OPAQUE2,ZmNRT1.1,ZmIAA12,ZmIAA19,and ZmbZIP20,repressing their expressions.Our study demonstrates that ZmEREB167 functions as a negative regulator in maize endosperm development and affects starch accumulation and kernel size.展开更多
Petrochemical plastics are widely used for food protection and preservation;however,they exhibit poor biodegradability,resisting natural degradation through physical,chemical,or enzymatic processes.As a sustainable al...Petrochemical plastics are widely used for food protection and preservation;however,they exhibit poor biodegradability,resisting natural degradation through physical,chemical,or enzymatic processes.As a sustainable alternative to conventional plastic packaging,edible films offer effective barriers against moisture,gases,and microbial contamination while being biodegradable,biocompatible,and environmentally friendly.In this study,novel active food packaging materials(in film form)were developed by incorporating starch,carrageenan,nanocellulose(NC),Aloe vera,and hibiscus flower extract.The effects of varying the matrix composition(26.5–73.5 wt.%starch/carrageenan),NC concentration(2.77-17.07 wt.%),and particle type(fibers or crystals)on the film structure and characteristics were analyzed using various methods.Scanning electron microscopy demonstrated good homogeneity and effective dispersion of NC within the blendmatrix.An increased carrageenan content in the filmimproved wettability,moisture absorption,solubility,and water vapor permeability.The mechanical properties of the films were enhanced by NC incorporation and higher carrageenan content.The developed films also exhibited effective UV radiation barriers and biodegradability.Films with low carrageenan content(less than 33.3%)and high NC content(7%,10% crystals or 10%,15% fibers)exhibited optimal properties,including enhanced water resistance,hydrophobicity,and mechanical strength,along with reduced water vapor permeability.However,the high water solubility and moisture absorption(above 55% and 14%,respectively)indicated their unsuitability as packaging materials for food products with wet surfaces and high humidity.The results suggest that these films are well suited for use as edible food packaging for fruits and vegetables.展开更多
Nitrogen fertilizer is an important agronomic measure significantly affecting crop yield and grain quality.This twoyear study aimed to explore the effects of four nitrogen levels on the morphology,lamellar and crystal...Nitrogen fertilizer is an important agronomic measure significantly affecting crop yield and grain quality.This twoyear study aimed to explore the effects of four nitrogen levels on the morphology,lamellar and crystalline structure,pasting,and rheological properties of proso millet(PM)starch and to investigate potential food applications of PM.The results showed that the starch surface became uneven,and the structure of the starch granules shifted towards greater complexity with increasing nitrogen levels.Nitrogen increased the relative crystallinity,ordered structure,and average repeat distance,leading to a stable starch structure and a higher gelatinization enthalpy.Furthermore,nitrogen significantly increased peak,breakdown,setback,and final viscosities but decreased apparent amylose content,which caused the shear resistance and storage capacity of PM starch-based foods to deteriorate during processing and transport.Rheological analysis showed that PM starch exhibited the typical characteristics of a pseudoplastic fluid.Under nitrogen treatment,PM starch gels showed high potential for application in 3D printing products due to high resistance to shear thinning,gel strength,and yield stress,presumably owing to the significantly higher G′than G′′,and the lower amylose content,which in turn promoted the formation of a stable network structure in water to immobilize more water.Our study provides a reference for applying PM starch in the food industry and for developing PM cropping-management programs to improve quality.展开更多
The impacts of drought stress on crop yield and quality are substantial. Drought priming during the early growth stage of plants has been shown to improve tolerance to drought stress during the reproductive stage, alt...The impacts of drought stress on crop yield and quality are substantial. Drought priming during the early growth stage of plants has been shown to improve tolerance to drought stress during the reproductive stage, although its effects on grain quality remain elusive. This study investigated the influence of drought priming on starch and protein levels in grains under drought stress during grain filling. Our results revealed that drought stress leads to a reduction in the contents of starch and its constituents, while simultaneously increasing glutenin macropolymers and protein fractions. Notably, drought primed plants under drought stress(PD) exhibited mitigated declines in the contents of starch and its components, leading to improvements in starch swelling power and pasting properties. In addition, PD resulted in a slight increase in the protein fractions, limiting the overall rise in total protein content compared to drought stress alone. The results of our study underscore the efficacy of drought priming as a strategy to counteract the negative effects of drought stress on grain quality, particularly by minimizing starch losses and restraining protein content elevation.展开更多
Fermented corn starch has emerged as a promising functional food due to its triad of gut biotics,prebiotic,probiotic,and postbiotic properties,which present significant potential for the management of type 2 diabetes ...Fermented corn starch has emerged as a promising functional food due to its triad of gut biotics,prebiotic,probiotic,and postbiotic properties,which present significant potential for the management of type 2 diabetes through gut microbiota modulation.During fermentation,microbial activity alters the starch matrix,enhancing the production of bioactive compounds such as resistant starch,isomalto-oligosaccharides,and resistant dextrin,which improve insulin sensitivity,reduce inflammation,and support glycemic control.Additionally,fermented corn starch harbors beneficial microbial strains including Lactiplantibacillus fermentum,Bifidobacterium breve,and Saccharomyces cerevisiae,which reinforce gut barrier integrity,stimulate incretin secretion,and suppress systemic inflammation.Postbiotic metabolites such as short-chain fatty acids,exopolysaccharides,and bacteriocins further contribute to glucose homeostasis through immune modulation and gut hormone regulation.Despite its promise,the clinical translation of fermented corn starch is limited by safety concerns(e.g.,contamination with pathogens or mycotoxins),lack of standardized fermentation protocols,and a scarcity of targeted studies.This review synthesizes current evidence on the antidiabetic potential of fermented corn starch,advocating for its integration into precision nutrition approaches and supporting further research to address safety and standardization challenges in functional food development.展开更多
[Objectives]To explore the methods for identifying pure honey.[Methods]Using 35 batches of Jiulongteng honey sourced from various production areas in Guangxi as the research subjects,this study investigated the chromo...[Objectives]To explore the methods for identifying pure honey.[Methods]Using 35 batches of Jiulongteng honey sourced from various production areas in Guangxi as the research subjects,this study investigated the chromogenic reactions of starch and dextrin,as well as the comparative study of thin-layer chromatography of oligosaccharides present in Jiulongteng honey.[Results]None of the 35 batches of Jiulongteng honey samples exhibited blue(indicating starch),green,or reddish-brown(indicating dextrin)coloration,suggesting that no adulterants such as artificially added starch,dextrin,or sugar were present in these samples.Furthermore,none of the 35 batches displayed additional spots below the corresponding positions of the control,indicating that the sugar composition was consistent with the oligosaccharide profile of natural honey.No components inconsistent with the oligosaccharide profile of natural honey were detected.Therefore,it can be concluded that the Jiulongteng honey samples in this experiment were pure and free from adulteration with starch,dextrin,or other sugar substances.[Conclusions]The method employed in this experiment is straightforward and quick to implement,effectively preventing adulterated honey from entering the market.It enhances the efficiency of quality control for Jiulongteng honey and promotes the healthy development of the Jiulongteng honey industry.展开更多
Resistant starch(RS)has attracted much researchers'attention because of its health-beneficial effects.Gut microbiota obviously shapes human health,but the effects of RS supplementation on the changes of human gut ...Resistant starch(RS)has attracted much researchers'attention because of its health-beneficial effects.Gut microbiota obviously shapes human health,but the effects of RS supplementation on the changes of human gut microbiota remain unclear.This observational meta-analysis aimed to reveal the effects of RS intake onα-diversity and composition of gut microbiota through meta-analysis.Two independent authors systematically searched articles from inception until February 2023 on four electronic databases.Twenty-four highly relevant trials were included conforming to Preferred Reporting Items for Systematic reviews and MetaAnalysis protocol,and a total of 816 individuals were included.Significant heterogeneity analyses revealed that RS intake notably decreased Shannon index(weighted mean difference(WMD):-0.11;95%confidence interval(CI):-0.21,-0.01),and stimulated the composition of health promoting bacteria genera,including Bifidobacterium(relative abundance:WMD:1.75;95%CI:0.39,3.11;bacterial populations:WMD:0.36;95%CI:0.04,0.69),Faecalibacterium(relative abundance:WMD:0.70;95%CI:0.20,1.20;fold change:effect size:0.91;95%CI:0.60,1.23),and Prevotella(relative abundance:WMD:0.35;95%CI:0.01,0.69).Taken together,the present study revealed that changes in the gut microbiota diversity and genera were correlated with RS supplementation,which may contribute to benefits in human health.展开更多
This is a review on resistant starch(RS),resistant dextrin(RD),and polydextrose(PDX),focusing on their similarities and differences.RS refers to the starch(or a portion of)that cannot be digested in the small intestin...This is a review on resistant starch(RS),resistant dextrin(RD),and polydextrose(PDX),focusing on their similarities and differences.RS refers to the starch(or a portion of)that cannot be digested in the small intestine,but can be partially fermented in the colon.The enzyme resistance of RS is mainly due to either its crystalline/granular structure or its interaction with other components.RD is produced by pyrodextrinization of starch,while PDX is produced by polycondensation of glucose and sorbitol.Both RD and PDX contain glycosidic linkages that are not digestible by the enzymes in the small intestine.RS is not soluble in water,whereas RD and PDX are soluble,mainly due to their molecular structures and other structural features.The major health benefits of RS,RD,and PDX are quite similar,including gut health,prebiotic effects,glycemic control,weight management,and prevention of cardiovascular disease.However,the efficacies can be different among them,for example,the degree and rate of gut fermentation.This review compares the definitions,functional properties,and health benefits of RS,RD,and PDX with the underlying mechanisms,which can be useful for their incorporation in food formulations to improve human health and wellness.展开更多
Starch retrogradation is a primary contributor to the staling of bread.The impact of the surfactant glyceryl monostearate(GMS)on the structure and retrogradation characteristics of wheat starch gels was explored durin...Starch retrogradation is a primary contributor to the staling of bread.The impact of the surfactant glyceryl monostearate(GMS)on the structure and retrogradation characteristics of wheat starch gels was explored during storage at 4℃ for 2 h and 5 d.In this research,the incorporation of GMS(0.14%,W/W,based on flour weight)significantly reduced the crumb firmness(P<0.05).Molecular dynamics simulation vividly demonstrated the formation process of the single-helical amylose-GMS complex within 150 ns during the short-term retrogradation process of cooling the gelatinized starch for 2 h.X-ray diffraction analysis revealed that GMS slightly decreased the relative crystallinity of starch from 8.9%to 7.8%during long-term retrogradation.The reduction in R1047/1022 detected by Fourier-transform infrared spectroscopy indicated that GMS could reduce the degree of retrogradation.Solid-state ^(13)C NMR analysis showed a characteristic resonance peak at 31.7 ppm for the GMSstarch complex.This study indicates that GMS holds great application potential in retarding starch retrogradation.展开更多
Retrogradation of semi-dry rice noodles severely reduced their eating quality during storage.Three commonly used modified starches(oxidized starch,acetylated starch,and hydroxypropyl starch)were applied to investigate...Retrogradation of semi-dry rice noodles severely reduced their eating quality during storage.Three commonly used modified starches(oxidized starch,acetylated starch,and hydroxypropyl starch)were applied to investigate the anti-retrogradation effects of semi-dry rice noodles during cold storage.Loss of water content,migration of water,and increase in relative crystallinity,retrogradation enthalpy,and hardness could be retarded by adding chemically modified starches,especially hydroxypropyl starches.The effect of hydroxypropyl starch addition levels(2%,4%,6%,8%,and 10%)on the properties of rice flour and the edible quality of semi-dry rice noodles was further evaluated.The water solubility index of rice flour decreased with the addition of hydroxypropyl starch,while the swelling power showed the opposite trend.The quality of semi-dry rice noodles were improved with the addition of hydroxypropyl starch.Compared to the control,semi-dried rice noodles with 8%hydroxypropyl starch possessed superior properties,i.e.lower cooking loss(decreasing from 12.89%to 6.62%),lower adhesiveness(decreasing from 5.40 to 4.31 g·s),and higher hardness(rising from 10.89 to 13.81 N).These findings demonstrated that the incorporation of hydroxypropyl starch is a promising strategy for the preparation of semi-dry rice noodles with satisfactory cooking and edible qualities as well as a long shelf life.展开更多
Postruminal intestinal inflammation and hindgut acidosis caused by increased dietary starch supply and thereby increased quantities of ruminal degradable starch(RDS)in ruminants have been widely studied.Although the r...Postruminal intestinal inflammation and hindgut acidosis caused by increased dietary starch supply and thereby increased quantities of ruminal degradable starch(RDS)in ruminants have been widely studied.Although the roles of the microbiota in mediating hindgut health that are focused on the hindgut have been widely studied,the absence of whole gastrointestinal insight may influence the depth of research.We integrated the microbiome,metabolome,and host transcriptome changes in the rumen,jejunum,ileum,and colon to investigate the contributions of foregut changes to hindgut gene expression driven by gastrointestinal microbiota and metabolite flow.Forty goats were randomly assigned to receive either a low rumen-degradation-rate starch diet(LRDS,n=20)or a high rumen-degradation-rate starch diet(HRDS,n=20).Compared with the HRDS group,the LRDS group significantly decreased the diarrheal rate.Based on the mean values of the fecal scores,6 represented goats of LRDS group(fecal scores=(4.58±0.120))and 6 represented goats of HRDS group(fecal scores=(3.53±0.343))were selected for sampling and subsequent analysis.LRDS had significantly decreased the colonic pathologic scores.Transcriptomic analysis revealed that LRDS reduced jejunal,ileal,and colonic inflammatory responses.An increase in beneficial commensals and a decreased abundance of pathogenic genera in the small intestine and hindgut were found in goats fed the LRDS diet using 16S rRNA gene sequencing.To identify microbial transmission as well as the transmission of microbial metabolites,8 genera were identified as core genera according to their calculated niche width.Metabolomics analysis revealed that a total of 554 metabolites were identified among different gastrointestinal sites.Then,metabolites were incorporated into 3 modules:metabolites increased in the current site(ICS),unchanged inflow metabolites in the current site(UICS),and metabolites decreased in the current site(DCS).The results indicated that the UICS metabolites contributed more than 10%to host gene expression in the jejunum,ileum,and colon.When we further focused on the effects of colonic UICS metabolites on the colonic immune-related differentially expressed genes(DEGs),the results indicated that 1-palmitoylglycerol and deoxycholic acid contributed 60.74 and 11.5%to the colonic immune-related DEGs,respectively.Our findings provide a preliminary framework of microbial effects that includes the microbiota and their metabolite changes,especially reduced 1-palmitoylglycerol and deoxycholic acid,in the former gastrointestinal tract that could be involved in the alleviation of colonic inflammation in goats fed LRDS diets.展开更多
文摘Starch is an essential commodity for humans and other animals.Future demands require qualitative and quantitative improvement by crop and post-harvest engineering that calls for comprehensive actions requiring increased fundamental knowledge on starch biosynthesis,development of advanced breeding strategies,efficient farming,and well-adapted and up scalable extraction protocols for diverse starch products.Recent staggering progress in molecular breeding techniques,especially genome editing,have enabled generation of higher starch yield and special functional qualities required to support such advancement.However,this necessitates fundamental biochemical and mechanistic understanding of starch biosynthesis and the variegated starch crop germplasms,all of which are closely linked to the relationships between starch molecular structures and functionality of various starch types as directed by the different capabilities of starch crop genotypes.We here review starch biosynthesis and its genetic foundation with a focus on increasing nutritional and health-promoting value of starch especially through bioengineering of the high amylose trait.
基金supported by the Major Program of National Agricultural Science and Technology of China(NK20220607)the Sichuan Science and Technology Program,China(2023YFH0041)。
文摘Starch biosynthesis is a complex process that relies on the coordinated action of multiple enzymes.Resistant starch is not digested in the small intestine,thus preventing a rapid rise in the glycemic index.Starch synthase 2a(SS2a)is a key enzyme in amylopectin biosynthesis that has significant effects on starch structure and properties.In this study,we identified an ss2a null mutant(M3-1413)with a single base mutation from an ethyl methane sulfonate(EMS)-mutagenized population of barley.The mutation was located at the 3'end of the first intron of the RNA splicing receptor(AG)site,and resulted in abnormal RNA splicing and two abnormal transcripts of ss2a,which caused the inactivation of the SS2a gene.The starch structure and properties were significantly altered in the mutant,with M3-1413 containing lower total starch and higher amylose and resistant starch levels.This study sheds light on the effect of barley ss2a null mutations on starch properties and will help to guide new applications of barley starch in the development of nutritious food products.
基金funded by the Thailand Science Research and Innovation(TSRI)under Fundamental Fund 2023(Project:Advanced Materials and Manufacturing for Applications in New S-Curve Industries).
文摘This work explores the development of biodegradable laminar composite foams for cushioning applications.The focus lies on overcoming the inherent brittleness of starch foams by incorporating various paper types as rein-forcement.Tapioca starch and glutinous starch were blended in varying ratios(100:0–0:100)to optimize the base material’s properties.The morphology,density,flexural strength,and impact strength of these starch blends were evaluated.The results revealed a trade-off between impact strength and density,with increasing glutinous starch content favoring impact resistance but also leading to higher density.The optimal ratio of tapioca to glutinous starch for achieving maximumflexural strength and modulus was determined to be 60:40.Theflexural strength of the composite material at this ratio reached a peak value of 5.3±0.6 MPa,significantly surpassing theflexural strength of pure tapioca foam,which was measured to be 3.5±0.4 MPa.Building on this foundation,novel lami-nar composite foams were fabricated using the 60:40 starch blend reinforced with mulberry paper,kraft paper,and newsprint paper.To enhance the interfacial adhesion between the starch matrix and paper reinforcement,a silane coupling agent was employed at a 10 wt%loading on the paper.The incorporation of paper reinforcement into starch foams was found to enhance their mechanical properties.Specifically,flexural strength values increased from 5.3±0.6 MPa for the unreinforced starch foam to 6.8±0.6 MPa,8.1±0.9 MPa,and 7.4±0.1 MPa when reinforced with mulberry paper,kraft paper,and newsprint paper,respectively.Notably,kraft paper reinforcement led to the most enhancements inflexural strength,flexural modulus,and impact strength.This research paves the way for developing sustainable cushioning materials with competitive mechanical properties using bio-based resources like starch and paper.
基金Industrial innovation team of Chuzhou CityFengyang County“Fengxiang”talent team+3 种基金Huainan City Science and Technology Plan Project(2023076)Innovation and Entrepreneurship Training Program for College Students(202410879085)the Natural Science Research Projects of Anhui Universities(811904)the Excellent Research and Innovation Team of Anhui Province Universities in 2024(2024AH010007)。
文摘With the promotion of potatoes as a staple food,the challenge lies in integrating them into traditional staple grain-based diets.As the primary component of grains,starch plays a crucial role,and its interactions with other heterogeneous starches could significantly influence their functional properties.Therefore,this study aimed to explore the role of substitution ratio(SR,0–100%)of potato starch(PS)in the compatibility with wheat starch(WS)from the perspective of microstructure and physicochemical characteristics using the simplex-centroid method.Results of scaning electron microscopy(SEM)revealed the network structure in SR 30%of PS possessed the smallest size and the most compact gel structure.A higher SR of PS increased the solubility and swelling power of the blended WS and improved its freeze-thaw stability.Peak viscosity and gel hardness of the blended WS exhibited an increasing trend as the SR of PS increased.With the increasing SR of PS,the blended WS exhibited an increased tanδvalue with decreased viscous and elastic moduli.There is a non-additive effect between PS and WS,which should be responsible for their difference between experimental and theoretical values.These findings could provide a new direction for understanding the compatibility interactions between different starches,and provide a theoretical guidance for the development of PS-based foods with desirable properties.
基金financially supported by the projects of National Key Research and Development Program of China(2021YFF1000203)the National Natural Science Foundation of China(32171924)+1 种基金the Support Plan for Scientific and Technological Innovation Talents in Colleges and Universities of Henan Province(23HASTIT038)the Graduate Joint Training Base Project of Henan Province(YJS2024JD18).
文摘Jasmonate ZIM-domain(JAZ)proteins are key repressors of the jasmonate signaling pathway and are involved in plant stress responses.However,their roles in starch biosynthesis in cereal crops remain unclear.In this study,we identified a locus associated with starch content on chromosome 5A by a genome-wide association study(GWAS).At this locus,a gene(TraesCS5A02G204900)encoding a JAZ protein(TaJAZ1)was found to be highly expressed in grains.CRISPR/Cas9-induced mutants were generated to investigate the role of TaJAZ1 in starch biosynthesis.Phenotypic characterization revealed significant alterations in starch granule size,crystallinity,and digestibility.Specifically,the two mutant lines(tajaz1-abd#1 and tajaz1-abd#2)exhibited increased total starch(12.5%and 17.6%,respectively),amylose(79.3%and 72.1%,respectively),resistant starch(88.5%and 96.8%,respectively),and grain yield per plant(103.8%and 58.8%,respectively).Furthermore,the mutation of TaJAZ1 significantly increased the expression levels of TaSBEI,TaAGPS1,TaAGPL1 and TaGBSSI,but decreased the expression levels of TaSSIIa,TaSSIIb and TaSBEIIa by binding to their promoters.Taken together,our results demonstrate that TaJAZ1 is a negative regulator of starch biosynthesis and grain yield.These findings not only provide novel insights into wheat starch biosynthesis regulation,but also contribute to potential genes for breeding wheat varieties of better quality and higher yield.
文摘Dynamic changes of starch, amylose, sucrose contents and the activities of starch synthesis enzymes under shading treatments alter flowering were studied using two rice varieties IR72 （indica） and Nipponbare （japonica） as materials. Under shading treatments, the starch, amylose and sucrose contents decreased, while ADP-glucose pyrophosphorylase （ADPGPPase） activity only changed a little, soluble starch synthase activity and granule bound starch synthase activity decreased, soluble starch branching enzyme （SSBE, Q-enzyme） activity and granule bound starch branching enzyme （GBSBE, Q-enzyme） activity increased, and starch debranching enzyme （DBE, R-enzyme） activity varied with varieties. Correlation analyses showed that the changes of starch content were positively and significantly correlated with the changes of sucrose content in the weak light. Both ADPGPPase activity and SSBE activity were positively and significantly correlated with starch accumulation rate. It was implied that the decline of starch synthase activities was related to the decrease of starch content and the increase of the activity of starch branching enzyme played an important role in the decrease of the ratio of amylose to the total starch under the weak light.
文摘To study the characteristics of tuber starchgranules,firstly,three kinds of common tuber starches were separated and characterized by gravitational field-flow fractionation(GrFFF)instrument,and the ratios of large and small starch granules A/B were calculated.Then,the liquid of peak A and peak B was collected and observed by scanning electron microscope(SEM)to verify the separation result of GrFFF.Secondly,the particle size distribution and crystal characteristics were characterized by particle size analyser,X-ray diffraction(X-ray)and fourier transform infrared spectroscopy(FT-IR).Three kinds of tuber starches all had the bimodal distribution in GrFFF.Peak A contained large particles,and peak B contained small particles.For yam starch,the ratio of starch granules A/B was much higher than 1;for sweet potato starch the ratio was approximately equal to 1;for tapioca starch,the ratio was much lower than 1.Moreover,the starch granules of sweet potato and tapioca were both A-type crystallinity,while yam starch granules were C-type crystallinity.GrFFF could be used to characterize and separate large and small granules of tuber starches and estimate the ratio of starch granules A/B.Yam starch was suitable for the development of slowly digestible starch(one kind of functional food),which could delay the occurrence of metabolic syndrome,diabetes and cardiovascular diseases.Tapioca starch was suitable for plant capsules,which were safer than gelatin capsule and could prevent the"poison capsule"incident from happening.
基金supported by the National Key R&D Program of China(2021YFD1300404)。
文摘Background The synchronized absorption of amino acids(AAs)and glucose in the gut is crucial for effective AA utilization and protein synthesis in the body.The study investigated how the starch digestion rate and AA levels impact intestinal AA digestion,transport and metabolism,breast muscle protein metabolism,and growth in grower broilers.A total of 72021-day-old healthy male Arbor Acres Plus broilers were randomly assigned to 12 treatments,each with 6 replicates of 10 birds.The treatments comprised 3 different starch[corn:control,cassava:rapidly digestible starch(RDS),and pea:slowly digestible starch(SDS)]with 4 different AA levels[based on standardized ileal digestible lysine(SID Lys),0.92%,1.02%(as the standard),1.12%and 1.22%].Results An interaction between dietary starch sources and SID Lys levels significantly affected breast muscle yield(P=0.033).RDS and SDS diets,or SID Lys levels of 0.92%,1.02%,or 1.22%,significantly decreased the breast muscle yield of broilers in contrast to the corn starch diet with 1.12%SID Lys(P=0.033).The SID Lys levels of 1.12%and 1.22%markedly improved body weight(BW),body weight gain(BWG)from 22 to 42 days of age,and mRNA expression of y^(+)LAT1 and mTOR while reducing feed intake(FI)and feed/gain ratio(F/G)compared to the 0.92%SID Lys level(P<0.05).The SDS diet significantly decreased BW and BWG of broilers from 22 to 42 days of age,distal ileal starch digestibility,jejunal amylase and chymotrypsin activities,and mRNA expression of GLUT2 and y^(+)LAT1 compared to the corn starch diet(P<0.05).The RDS diet suppressed the breast muscle mass by down-regulating expression of mTOR,S6K1,and eIF4E and up-regulating expression of MuRF,CathepsinB,Atrogin-1,and M-calpain compared to the corn starch diet(P<0.05).Targeted metabolomics analysis revealed that the SDS diet significantly increased acetyl-CoA andα-ketoglutaric acid levels in the tricarboxylic acid(TCA)cycle(P<0.05)but decreased the ileal digestibility of Lys,Tyr,Leu,Asp,Ser,Gly,Pro,Arg,Ile,and Val compared to the corn starch group(P<0.05).Conclusion The SDS diet impaired broiler growth by reducing intestinal starch digestibility,which inhibited intestinal AA and glucose absorption and utilization,increased AA oxidation for energy supply,and lowered the efficiency of protein synthesis.Although the RDS diet resulted in growth performance similar to the corn starch diet,it reduced breast muscle mass by inhibiting protein synthesis and promoting degradation.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.31830071,32202561)the earmarked fund for CARS(Grant No.CARS-31)。
文摘Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation,epigenetic factor,and post-translational modification.Previously,we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation.However,whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown.In this study,we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence(DAP-Seq)approach.Electrophoretic mobility shift assay(EMSA)and dual-luciferase reporter assay(DLR)demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3,MaBAM2 and MaGWD1.Further analyses of yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and Luciferase complementation imaging(LCI)assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1,and this interaction weakened the MaERF11-mediated transcriptional repression capacity.Collectively,our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.
基金supported by STI 2030-Major Project(2023ZD04069)National Key Research and Development Program of China(2023YFD1202900)+3 种基金The National Science Fund for Distinguished Young Scholars(32425041)The“Breakthrough”Science and Technology Project of Tongliao(TL2024TW001)Science and Technology Demonstration Project of Shandong Province(2024SFGC0402)Pinduoduo-China Agricultural University Research Fund(PC2023A01004).
文摘Transcription factors play critical roles in the regulation of gene expression during maize kernel development.The maize endosperm,a large storage organ,accounting for nearly 90%of the dry weight of mature kernels,serves as the primary site for starch storage.In this study,we identify an endosperm-specific EREB gene,ZmEREB167,which encodes a nucleus-localized EREB protein.Knockout of ZmEREB167 significantly increases kernel size and weight,as well as starch and protein content,compared with the wild type.In situ hybridization experiments show that ZmEREB167 is highly expressed in the BETL as well as PED regions of maize kernels.Dual-luciferase assays show that ZmEREB167 exhibits transcriptionally repressor activity in maize protoplasts.Transcriptome analysis reveals that a large number of genes are up-regulated in the Zmereb167-C1 mutant compared with the wild type,including key genetic factors such as ZmMRP-1 and ZmMN1,as well as multiple transporters involved in maize endosperm development.Integration of RNA-seq and ChIP-seq results identify 68 target genes modulated by ZmEREB167.We find that ZmEREB167 directly targets OPAQUE2,ZmNRT1.1,ZmIAA12,ZmIAA19,and ZmbZIP20,repressing their expressions.Our study demonstrates that ZmEREB167 functions as a negative regulator in maize endosperm development and affects starch accumulation and kernel size.
基金funded by the Russian Federation represented by the Ministry of Science and Higher Education,Russia,grant number 075-15-2022-1231 on 18.10.2022National Research Foundation(NRF),South Africa,grant number 150508Brazilian National Council for Scientific and Technological Development(CNPq),Brazil,grant number 440057/2022-1.
文摘Petrochemical plastics are widely used for food protection and preservation;however,they exhibit poor biodegradability,resisting natural degradation through physical,chemical,or enzymatic processes.As a sustainable alternative to conventional plastic packaging,edible films offer effective barriers against moisture,gases,and microbial contamination while being biodegradable,biocompatible,and environmentally friendly.In this study,novel active food packaging materials(in film form)were developed by incorporating starch,carrageenan,nanocellulose(NC),Aloe vera,and hibiscus flower extract.The effects of varying the matrix composition(26.5–73.5 wt.%starch/carrageenan),NC concentration(2.77-17.07 wt.%),and particle type(fibers or crystals)on the film structure and characteristics were analyzed using various methods.Scanning electron microscopy demonstrated good homogeneity and effective dispersion of NC within the blendmatrix.An increased carrageenan content in the filmimproved wettability,moisture absorption,solubility,and water vapor permeability.The mechanical properties of the films were enhanced by NC incorporation and higher carrageenan content.The developed films also exhibited effective UV radiation barriers and biodegradability.Films with low carrageenan content(less than 33.3%)and high NC content(7%,10% crystals or 10%,15% fibers)exhibited optimal properties,including enhanced water resistance,hydrophobicity,and mechanical strength,along with reduced water vapor permeability.However,the high water solubility and moisture absorption(above 55% and 14%,respectively)indicated their unsuitability as packaging materials for food products with wet surfaces and high humidity.The results suggest that these films are well suited for use as edible food packaging for fruits and vegetables.
基金supported by the Postdoctoral Fellowship Program of China Postdoctoral Science Foudation(CPSF)(GZC20241394)the Shaanxi Province Natural Science Basic Research Program-Youth Project,China(2025JCYBQN271)+2 种基金the National Natural Science Foundation of China(31371529)the Shaanxi Province“Two Chains”Integrated Crop Breeding Key Project,China(2021LLRH07)the Minor Grain Crops Research and Development System of Shaanxi Province,China(NYKJ-2021YL(XN)40)。
文摘Nitrogen fertilizer is an important agronomic measure significantly affecting crop yield and grain quality.This twoyear study aimed to explore the effects of four nitrogen levels on the morphology,lamellar and crystalline structure,pasting,and rheological properties of proso millet(PM)starch and to investigate potential food applications of PM.The results showed that the starch surface became uneven,and the structure of the starch granules shifted towards greater complexity with increasing nitrogen levels.Nitrogen increased the relative crystallinity,ordered structure,and average repeat distance,leading to a stable starch structure and a higher gelatinization enthalpy.Furthermore,nitrogen significantly increased peak,breakdown,setback,and final viscosities but decreased apparent amylose content,which caused the shear resistance and storage capacity of PM starch-based foods to deteriorate during processing and transport.Rheological analysis showed that PM starch exhibited the typical characteristics of a pseudoplastic fluid.Under nitrogen treatment,PM starch gels showed high potential for application in 3D printing products due to high resistance to shear thinning,gel strength,and yield stress,presumably owing to the significantly higher G′than G′′,and the lower amylose content,which in turn promoted the formation of a stable network structure in water to immobilize more water.Our study provides a reference for applying PM starch in the food industry and for developing PM cropping-management programs to improve quality.
基金supported by the projects of the National Key Research and Development Program of China (2023YFD2300202)the National Natural Science Foundation of China (32272213 and 31771693)+1 种基金the China Agriculture Research System (CARS-03)the Jiangsu Collaborative Innovation Center for Modern Crop Production, China (JCIC-MCP)。
文摘The impacts of drought stress on crop yield and quality are substantial. Drought priming during the early growth stage of plants has been shown to improve tolerance to drought stress during the reproductive stage, although its effects on grain quality remain elusive. This study investigated the influence of drought priming on starch and protein levels in grains under drought stress during grain filling. Our results revealed that drought stress leads to a reduction in the contents of starch and its constituents, while simultaneously increasing glutenin macropolymers and protein fractions. Notably, drought primed plants under drought stress(PD) exhibited mitigated declines in the contents of starch and its components, leading to improvements in starch swelling power and pasting properties. In addition, PD resulted in a slight increase in the protein fractions, limiting the overall rise in total protein content compared to drought stress alone. The results of our study underscore the efficacy of drought priming as a strategy to counteract the negative effects of drought stress on grain quality, particularly by minimizing starch losses and restraining protein content elevation.
基金Supported by the Research Office,University of KwaZulu-Natal,Durbanan Incentive Grant from the National Research Foundation,Pretoria,South Africa,No.145943.
文摘Fermented corn starch has emerged as a promising functional food due to its triad of gut biotics,prebiotic,probiotic,and postbiotic properties,which present significant potential for the management of type 2 diabetes through gut microbiota modulation.During fermentation,microbial activity alters the starch matrix,enhancing the production of bioactive compounds such as resistant starch,isomalto-oligosaccharides,and resistant dextrin,which improve insulin sensitivity,reduce inflammation,and support glycemic control.Additionally,fermented corn starch harbors beneficial microbial strains including Lactiplantibacillus fermentum,Bifidobacterium breve,and Saccharomyces cerevisiae,which reinforce gut barrier integrity,stimulate incretin secretion,and suppress systemic inflammation.Postbiotic metabolites such as short-chain fatty acids,exopolysaccharides,and bacteriocins further contribute to glucose homeostasis through immune modulation and gut hormone regulation.Despite its promise,the clinical translation of fermented corn starch is limited by safety concerns(e.g.,contamination with pathogens or mycotoxins),lack of standardized fermentation protocols,and a scarcity of targeted studies.This review synthesizes current evidence on the antidiabetic potential of fermented corn starch,advocating for its integration into precision nutrition approaches and supporting further research to address safety and standardization challenges in functional food development.
基金Supported by Enhancement Project of Basic Scientific Research Ability of Young and Middle-aged Teachers in Guangxi Universities(2020KY07040)School-level Scientific Research Project of Guangxi University of Chinese Medicine(2024QN022)Self-financed Scientific Research Project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(GXZYL20240818).
文摘[Objectives]To explore the methods for identifying pure honey.[Methods]Using 35 batches of Jiulongteng honey sourced from various production areas in Guangxi as the research subjects,this study investigated the chromogenic reactions of starch and dextrin,as well as the comparative study of thin-layer chromatography of oligosaccharides present in Jiulongteng honey.[Results]None of the 35 batches of Jiulongteng honey samples exhibited blue(indicating starch),green,or reddish-brown(indicating dextrin)coloration,suggesting that no adulterants such as artificially added starch,dextrin,or sugar were present in these samples.Furthermore,none of the 35 batches displayed additional spots below the corresponding positions of the control,indicating that the sugar composition was consistent with the oligosaccharide profile of natural honey.No components inconsistent with the oligosaccharide profile of natural honey were detected.Therefore,it can be concluded that the Jiulongteng honey samples in this experiment were pure and free from adulteration with starch,dextrin,or other sugar substances.[Conclusions]The method employed in this experiment is straightforward and quick to implement,effectively preventing adulterated honey from entering the market.It enhances the efficiency of quality control for Jiulongteng honey and promotes the healthy development of the Jiulongteng honey industry.
基金financially supported by the National Key R&D Program of China(2022YFF1100600,2022YFF1100605)the Science and Technology Support Program(Modern Agriculture)of Jiangsu Province(BE2022323)the National Natural Science Foundation of China(32302011)。
文摘Resistant starch(RS)has attracted much researchers'attention because of its health-beneficial effects.Gut microbiota obviously shapes human health,but the effects of RS supplementation on the changes of human gut microbiota remain unclear.This observational meta-analysis aimed to reveal the effects of RS intake onα-diversity and composition of gut microbiota through meta-analysis.Two independent authors systematically searched articles from inception until February 2023 on four electronic databases.Twenty-four highly relevant trials were included conforming to Preferred Reporting Items for Systematic reviews and MetaAnalysis protocol,and a total of 816 individuals were included.Significant heterogeneity analyses revealed that RS intake notably decreased Shannon index(weighted mean difference(WMD):-0.11;95%confidence interval(CI):-0.21,-0.01),and stimulated the composition of health promoting bacteria genera,including Bifidobacterium(relative abundance:WMD:1.75;95%CI:0.39,3.11;bacterial populations:WMD:0.36;95%CI:0.04,0.69),Faecalibacterium(relative abundance:WMD:0.70;95%CI:0.20,1.20;fold change:effect size:0.91;95%CI:0.60,1.23),and Prevotella(relative abundance:WMD:0.35;95%CI:0.01,0.69).Taken together,the present study revealed that changes in the gut microbiota diversity and genera were correlated with RS supplementation,which may contribute to benefits in human health.
文摘This is a review on resistant starch(RS),resistant dextrin(RD),and polydextrose(PDX),focusing on their similarities and differences.RS refers to the starch(or a portion of)that cannot be digested in the small intestine,but can be partially fermented in the colon.The enzyme resistance of RS is mainly due to either its crystalline/granular structure or its interaction with other components.RD is produced by pyrodextrinization of starch,while PDX is produced by polycondensation of glucose and sorbitol.Both RD and PDX contain glycosidic linkages that are not digestible by the enzymes in the small intestine.RS is not soluble in water,whereas RD and PDX are soluble,mainly due to their molecular structures and other structural features.The major health benefits of RS,RD,and PDX are quite similar,including gut health,prebiotic effects,glycemic control,weight management,and prevention of cardiovascular disease.However,the efficacies can be different among them,for example,the degree and rate of gut fermentation.This review compares the definitions,functional properties,and health benefits of RS,RD,and PDX with the underlying mechanisms,which can be useful for their incorporation in food formulations to improve human health and wellness.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(LQ23C200003)National Natural Science Foundation of China(32202076)Natural Science Foundation of Shandong Province(Project No.ZR2023QC083).
文摘Starch retrogradation is a primary contributor to the staling of bread.The impact of the surfactant glyceryl monostearate(GMS)on the structure and retrogradation characteristics of wheat starch gels was explored during storage at 4℃ for 2 h and 5 d.In this research,the incorporation of GMS(0.14%,W/W,based on flour weight)significantly reduced the crumb firmness(P<0.05).Molecular dynamics simulation vividly demonstrated the formation process of the single-helical amylose-GMS complex within 150 ns during the short-term retrogradation process of cooling the gelatinized starch for 2 h.X-ray diffraction analysis revealed that GMS slightly decreased the relative crystallinity of starch from 8.9%to 7.8%during long-term retrogradation.The reduction in R1047/1022 detected by Fourier-transform infrared spectroscopy indicated that GMS could reduce the degree of retrogradation.Solid-state ^(13)C NMR analysis showed a characteristic resonance peak at 31.7 ppm for the GMSstarch complex.This study indicates that GMS holds great application potential in retarding starch retrogradation.
基金funded by the fund of national natural science foundation of China(32101996,32160530)the training plan for academic and technical leaders of major disciplines in Jiangxi province(20232BCJ23024)+1 种基金the key research and development program of Jiangxi province(20232BBF60023)“Shuangqian Project”of Jiangxi Province(jxsq2023201042)。
文摘Retrogradation of semi-dry rice noodles severely reduced their eating quality during storage.Three commonly used modified starches(oxidized starch,acetylated starch,and hydroxypropyl starch)were applied to investigate the anti-retrogradation effects of semi-dry rice noodles during cold storage.Loss of water content,migration of water,and increase in relative crystallinity,retrogradation enthalpy,and hardness could be retarded by adding chemically modified starches,especially hydroxypropyl starches.The effect of hydroxypropyl starch addition levels(2%,4%,6%,8%,and 10%)on the properties of rice flour and the edible quality of semi-dry rice noodles was further evaluated.The water solubility index of rice flour decreased with the addition of hydroxypropyl starch,while the swelling power showed the opposite trend.The quality of semi-dry rice noodles were improved with the addition of hydroxypropyl starch.Compared to the control,semi-dried rice noodles with 8%hydroxypropyl starch possessed superior properties,i.e.lower cooking loss(decreasing from 12.89%to 6.62%),lower adhesiveness(decreasing from 5.40 to 4.31 g·s),and higher hardness(rising from 10.89 to 13.81 N).These findings demonstrated that the incorporation of hydroxypropyl starch is a promising strategy for the preparation of semi-dry rice noodles with satisfactory cooking and edible qualities as well as a long shelf life.
基金supported by the National Natural Science Foundation of China(32072761,31902184 and 32102570)the Shaanxi Provincial Science and Technology Association Young Talents Lifting Program Project,China(20220203)。
文摘Postruminal intestinal inflammation and hindgut acidosis caused by increased dietary starch supply and thereby increased quantities of ruminal degradable starch(RDS)in ruminants have been widely studied.Although the roles of the microbiota in mediating hindgut health that are focused on the hindgut have been widely studied,the absence of whole gastrointestinal insight may influence the depth of research.We integrated the microbiome,metabolome,and host transcriptome changes in the rumen,jejunum,ileum,and colon to investigate the contributions of foregut changes to hindgut gene expression driven by gastrointestinal microbiota and metabolite flow.Forty goats were randomly assigned to receive either a low rumen-degradation-rate starch diet(LRDS,n=20)or a high rumen-degradation-rate starch diet(HRDS,n=20).Compared with the HRDS group,the LRDS group significantly decreased the diarrheal rate.Based on the mean values of the fecal scores,6 represented goats of LRDS group(fecal scores=(4.58±0.120))and 6 represented goats of HRDS group(fecal scores=(3.53±0.343))were selected for sampling and subsequent analysis.LRDS had significantly decreased the colonic pathologic scores.Transcriptomic analysis revealed that LRDS reduced jejunal,ileal,and colonic inflammatory responses.An increase in beneficial commensals and a decreased abundance of pathogenic genera in the small intestine and hindgut were found in goats fed the LRDS diet using 16S rRNA gene sequencing.To identify microbial transmission as well as the transmission of microbial metabolites,8 genera were identified as core genera according to their calculated niche width.Metabolomics analysis revealed that a total of 554 metabolites were identified among different gastrointestinal sites.Then,metabolites were incorporated into 3 modules:metabolites increased in the current site(ICS),unchanged inflow metabolites in the current site(UICS),and metabolites decreased in the current site(DCS).The results indicated that the UICS metabolites contributed more than 10%to host gene expression in the jejunum,ileum,and colon.When we further focused on the effects of colonic UICS metabolites on the colonic immune-related differentially expressed genes(DEGs),the results indicated that 1-palmitoylglycerol and deoxycholic acid contributed 60.74 and 11.5%to the colonic immune-related DEGs,respectively.Our findings provide a preliminary framework of microbial effects that includes the microbiota and their metabolite changes,especially reduced 1-palmitoylglycerol and deoxycholic acid,in the former gastrointestinal tract that could be involved in the alleviation of colonic inflammation in goats fed LRDS diets.
