Staphylococcus lentus(S.lentus)is a coagulase negative gram positive cocci recognized as opportunistic pathogens and rarely forming biofilm;it has many virulence factors,but recently caused nosocomial and community in...Staphylococcus lentus(S.lentus)is a coagulase negative gram positive cocci recognized as opportunistic pathogens and rarely forming biofilm;it has many virulence factors,but recently caused nosocomial and community infections.Biofilm formation of Staphylococcus lentus may be associated with the ability to resist antibiotics which leads to increase in mortality rate due to the difficulty in eradicate infections.To evaluate the biofilm forming capacity of Staphylococcus lentus and its susceptibility to antibiotics,phenotypic and genotypic assays were used.Among 28 biofilm bacteria,Staphylococcus lentus was isolated and identified from urine catheterized patients who were hospitalized in different departments of four Iraqi hospitals(Al-Diwaniyah Teaching,Al-Hilla Teaching,Al Qassim and Al Hashimiyah Hospitals).Staphylococcus lentus was examined for detection of biofilm formation by detecting icaA gene,the intercellular adhesion gene which expressed adhesion factor to form biofilm in staphylococci by using polymerase chain reaction(PCR)method and tested for antimicrobial susceptibility by disc diffusion method and VITEK2 system according to guidelines of the Clinical&Laboratory Standards Institute(CLSI).Three isolates of Staphylococcus lentus revealed the ability to form biofilm phenotypically which contained icaA gene with 100%antibiotics resistance to penicillin,carbenicillin,gentamicin,tobramycin,oxacillin,vancomycin,clindamycin,ciprofloxacin,and 0%antibiotics resistance to azithromycin.icaA genes are present in Staphylococcus lentus and responsible for biofilm formation which is considered as the indicator;biofilm formation is a strong cause of multidrug resistance in bacteria.展开更多
目的研究炎症因子IL-6缺失是否影响鼻咽部菌群组成。方法提取鼻咽部灌洗液DNA并进行扩增子测序检测IL-6敲除前后鼻咽部菌群,分析菌群α多样性,并使用Wilcoxon秩和检验分析差异菌群。通过PICRUSt对菌群OTU丰富度表进行标准化,然后进行菌...目的研究炎症因子IL-6缺失是否影响鼻咽部菌群组成。方法提取鼻咽部灌洗液DNA并进行扩增子测序检测IL-6敲除前后鼻咽部菌群,分析菌群α多样性,并使用Wilcoxon秩和检验分析差异菌群。通过PICRUSt对菌群OTU丰富度表进行标准化,然后进行菌群关联的KEGG分析。结果IL-6基因敲除后鼻咽部菌群丰富度和多样性变化不明显,但菌群组成发生明显变化。丰富度前15个属中9个属明显变化,有6个属明显下降,3个属明显上升。其中,Streptococcus和Bergeyella丰富度明显升高,Staphylococcus、Rothia和Corynebacterium1丰富度明显降低。Real time PCR检测种水平发现IL-6敲除后Staphylococcus lentus丰富度明显降低。菌群KEGG分析发现,IL-6敲除后,炎性功能相关的脂肪细胞因子信号通路表达下调、细胞凋亡通路表达上调,另外,部分抗病毒活性通路表达上调。结论炎性因子IL-6与鼻咽部菌群组成存在明显相关性,菌群KEGG分析发现IL-6敲除后多个炎性功能相关通路表达受到影响。展开更多
文摘Staphylococcus lentus(S.lentus)is a coagulase negative gram positive cocci recognized as opportunistic pathogens and rarely forming biofilm;it has many virulence factors,but recently caused nosocomial and community infections.Biofilm formation of Staphylococcus lentus may be associated with the ability to resist antibiotics which leads to increase in mortality rate due to the difficulty in eradicate infections.To evaluate the biofilm forming capacity of Staphylococcus lentus and its susceptibility to antibiotics,phenotypic and genotypic assays were used.Among 28 biofilm bacteria,Staphylococcus lentus was isolated and identified from urine catheterized patients who were hospitalized in different departments of four Iraqi hospitals(Al-Diwaniyah Teaching,Al-Hilla Teaching,Al Qassim and Al Hashimiyah Hospitals).Staphylococcus lentus was examined for detection of biofilm formation by detecting icaA gene,the intercellular adhesion gene which expressed adhesion factor to form biofilm in staphylococci by using polymerase chain reaction(PCR)method and tested for antimicrobial susceptibility by disc diffusion method and VITEK2 system according to guidelines of the Clinical&Laboratory Standards Institute(CLSI).Three isolates of Staphylococcus lentus revealed the ability to form biofilm phenotypically which contained icaA gene with 100%antibiotics resistance to penicillin,carbenicillin,gentamicin,tobramycin,oxacillin,vancomycin,clindamycin,ciprofloxacin,and 0%antibiotics resistance to azithromycin.icaA genes are present in Staphylococcus lentus and responsible for biofilm formation which is considered as the indicator;biofilm formation is a strong cause of multidrug resistance in bacteria.
文摘目的研究炎症因子IL-6缺失是否影响鼻咽部菌群组成。方法提取鼻咽部灌洗液DNA并进行扩增子测序检测IL-6敲除前后鼻咽部菌群,分析菌群α多样性,并使用Wilcoxon秩和检验分析差异菌群。通过PICRUSt对菌群OTU丰富度表进行标准化,然后进行菌群关联的KEGG分析。结果IL-6基因敲除后鼻咽部菌群丰富度和多样性变化不明显,但菌群组成发生明显变化。丰富度前15个属中9个属明显变化,有6个属明显下降,3个属明显上升。其中,Streptococcus和Bergeyella丰富度明显升高,Staphylococcus、Rothia和Corynebacterium1丰富度明显降低。Real time PCR检测种水平发现IL-6敲除后Staphylococcus lentus丰富度明显降低。菌群KEGG分析发现,IL-6敲除后,炎性功能相关的脂肪细胞因子信号通路表达下调、细胞凋亡通路表达上调,另外,部分抗病毒活性通路表达上调。结论炎性因子IL-6与鼻咽部菌群组成存在明显相关性,菌群KEGG分析发现IL-6敲除后多个炎性功能相关通路表达受到影响。
